Changes of Biogenic Amine Level during Storage and Development of New Quality Index on Silkworm Pupa

식용 번데기의 저장 중 Biogenic Amine 함량 변화와 품질지표 개발

  • 조태용 (식품의약품안전청 위해기준팀) ;
  • 한규홍 (식품의약품안전청 식중독예방관리팀) ;
  • 강병선 (영동대학교 와인발효식품학과) ;
  • 김선봉 (부경대학교 식품공학과)
  • Published : 2007.12.31

Abstract

A study on the evolution of acid value, peroxide value and biogenic amines in silkworm pupa during 7 days at different temperature of storage (-18, 25 and $35^{\circ}C$) was performed. Seven biogenic amines (putrescine, cadaverine, histamine, tyramine, spermidine, spermine and 2-phenylethylamine) were determined. Acid value in Silkworm pupa increased both during normal temperature $(25^{\circ}C)$ and room temperature $(35^{\circ}C)$ and thebiogenic amine (histamine, tyramine) content generally increased in $25^{\circ}C\;and\;35^{\circ}C$ with storage time. Significant differences were found (P<0.05) in the levels of tyramine and histamine among Silkworm pupa. The relationship of storage time and acid value of Silkworm pupa were resolved a simple linear equation, and histamine and tyramine could be predicted using this equation. Quality indices related to the contents of the major biogenic amines were calculated and they correlated well with physicochemical characteristics qualities such as acid value.

본 연구는 식용 번데기를 냉동$(-18^{\circ}C)$, 상온$(25^{\circ}C)$ 및 실온$(35^{\circ}C)$에 저장하면서, 식용 번데기의 기준규격인 산가와 과산화물가의 경시변화를 살펴보고, biogenic amine의 함량과의 상관관계를 살펴봄으로써 식용 번데기의 새로운 품질지표로 biogenic amine을 검토하였다. 번데기 원료에서는 7개의 biogenic amine이 검출되었으며, 그 중 putrescine이 $243.0{\pm}5.4mg/kg$으로 가장 높게 나타났다. 저장기간에 따른 산가 및 과산화물가의 변화에 있어서는 냉동$(-18^{\circ}C)$에서는 저장 7일 동안 변화를 보이지 않았고, 상온$(25^{\circ}C)$에서는 2일, 실온$(35^{\circ}C)$에서는 2일 이전에 번데기 산가의 기준규격치인 5.0을 초과하였다. 또한, 상온$(25^{\circ}C)$에서 저장하는 동안 biogenic amine는 histamine과 tyramine이 유의적으로 증가하는 것으로 나타났고, 이를 바탕으로 산가와 histamine, tyramine의 상관관계를 살펴본 결과 저장기간과 산가는 simple linear식으로, 산가와 histamine, tyramine의 변화는 2차 simple linear관계식으로 제시되었다. 결과적으로 식용 번데기에 있어 산가 이외에 biogenic amine인 histamine과 tyramine이 유의적인 상관관계를 가짐으로써 새로운 품질지표로 사용이 가능하였다.

Keywords

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