• 미생물학회지
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• 제27권4호
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• pp.323-333
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• 1989

Transposon Tn5의 단백질 합성을 E. coli 내에서 증폭 합성시키기 위하여 Bacteriophage의 Pt 촉진유전자가 Tn5의 두 개 module인 IS50L 과 IS50R을 전사시킬 수 있도록 plasmid를 재구성하였다. P1 촉진유전자로부터의 전사를 탈억제시켰을 경우, IS50R으로부터 합성되는 두 개의 단백질은 모두 그 세포내 축적량이 SDS-polyacrylamid gel에서 확인 될 정도로 증폭합성되었으나 IS501L의 두 개 단백질들은 동일 gel 상에서 확인되지 않았다. Minicell system에서 합성양상과 각 단백질들의 안정성을 조사한 결과, IS50R 단백질은 모두 안정하게 유지되었으나 IS501, 단백질은 모두 불안정하여 생분해 되어 진다는 사실을 밝혔다. 이러한 Is50L 단백질의 불안정성은 IS50L이 transposition에 있어서 불활성을 나타내는 원인이라 추정된다. 또한 IS50L고 IS50R의 단백질은 모두 동일한 open reading frame에 의하여 합성되어짐을 tryptic peptide 양상을 통하여 알 수 있었다.

• Journal of Information Processing Systems
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• 제8권3호
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• pp.459-470
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• 2012

Recently, high-throughput technologies such as the two-hybrid system, protein chip, Mass Spectrometry, and the phage display have furnished a lot of data on protein-protein interactions (PPIs), but the data has not been accurate so far and the quantity has also been limited. In this respect, computational techniques for the prediction and validation of PPIs have been developed. However, existing computational methods do not take into account the fact that a PPI is actually originated from the interactions of domains that each protein contains. So, in this work, the information on domain modules of individual proteins has been employed in order to find out the protein interaction relationship. The system developed here, WASPI (Web-based Assistant System for Protein-protein interaction Inference), has been implemented to provide many functional insights into the protein interactions and their domains. To achieve those objectives, several preprocessing steps have been taken. First, the domain module information of interacting proteins was extracted by taking advantage of the InterPro database, which includes protein families, domains, and functional sites. The InterProScan program was used in this preprocess. Second, the homology comparison with the GO (Gene Ontology) and COG (Clusters of Orthologous Groups) with an E-value of $10^{-5}$, $10^{-3}$ respectively, was employed to obtain the information on the function and annotation of each interacting protein of a secondary PPI database in the WASPI. The BLAST program was utilized for the homology comparison.

• Steel and Composite Structures
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• 제30권2호
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• pp.185-199
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• 2019

Modular construction is an emerging technology to accommodate the increasing restrictions in terms of construction period, energy efficiency and environmental impacts, since each structural module is prefabricated offsite beforehand and assembled onsite using industrialized techniques. However, some innate structural drawbacks of this innovative method are also distinct, such as connection tying inaccessibility, column instability and system robustness. This study aims to explore the theoretical and numerical stability analysis of a tenon-connected square hollow section (SHS) steel column to address the tying and stability issue in modular construction. Due to the excellent performance of composite structures in fire resistance and buckling prevention, concrete-filled steel tube (CFST) columns are also taken into account in the analysis to evaluate the feasibility of adopting composite sections in modular buildings. Characteristic equations with three variables, i.e., the length ratio, the bending stiffness ratio and the rotational stiffness ratio, are generated from the fourth-order governing differential equations. The rotational stiffness ratio is recognized as the most significant factor, with interval analysis conducted for its mechanical significance and domain. Numerical analysis using ABAQUS is conducted for validation of characteristic equations. Recommendations and instructions in predicting the buckling performance of both SHS and CFST columns are then proposed.

• Geomechanics and Engineering
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• 제24권3호
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• pp.267-280
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• 2021

The research presented in this paper deals with dynamic stability analysis of the graphene nanoplatelets (GPLs) reinforced composite spinning disk. The presented small-scaled structure is simulated as a disk covered by viscoelastic substrate which is two-parametric. The centrifugal and Coriolis impacts due to the spinning are taken into account. The stresses and strains would be obtained using the first-order-shear-deformable-theory (FSDT). For Poisson ratio, as well as various amounts of mass densities, the mixture rule is employed, while a modified Halpin-Tsai model is inserted for achieving the elasticity module. The structure's boundary conditions (BCs) are obtained employing GPLs reinforced composite (GPLRC) spinning disk's governing equations applying principle of Hamilton which is based on minimum energy and ultimately have been solved employing numerical approach called generalized-differential quadrature-method (GDQM). Spinning disk's dynamic properties with different boundary conditions (BCs) are explained due to the curves drawn by Matlab software. Also, the simply-supported boundary conditions is applied to edges 𝜃=𝜋/2, and 𝜃=3𝜋/2, while, cantilever, respectively, is analyzed in R=R_i, and R₀. The final results reveal that the GPLs' weight fraction, viscoelastic substrate, various GPLs' pattern, and rotational velocity have a dramatic influence on the amplitude, and vibration behavior of a GPLRC rotating cantilevered disk. As an applicable result in related industries, the spinning velocity impact on the frequency is more effective in the higher radius ratio's amounts.

• Advances in Energy Research
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• 제8권1호
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• pp.21-39
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• 2022

In this study, the first law of thermodynamics was used to establish a one-dimensional (1-D) thermal model for parabolic trough receiver (PTR) taking into account the pressure drop and kinetic energy loss effects of the heat transfer fluid (HTF) flowing inside the absorber tube. The validation of the thermal model with data from the SEGS-LS2 solar collector-test showed a good agreement, which is consistent with the previously established models for the conventional straight and smooth (CSS) receiver where the effects of pressure drop and kinetic energy loss were neglected. Based on the developed model and code, a comparative study of the newly designed parabolic trough S-curved receiver versus the CSS receiver was conducted and solar unit's performances were analyzed. Without any supplementary devices, the S-curved receiver enhances the performance of the parabolic trough module, with a maximum of 0.16% compared to CSS receiver with the same sizes and mass flow rates. Thermal losses were reduced by 7% due to the decrease in the temperature of the outer surface of the receiver tube. In addition, it has been shown that from a mass flow rate of 9.5 kg/s the heat losses of the S-curved receiver remain unchanged despite the improvement in the heat transfer rate.

• 풍력에너지저널
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• 제2권2호
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• pp.30-37
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• 2011

A floating wind turbine dynamic simulation program, WindHydro, is newly developed taking into account wind inflow and incident wave. WindHydro consists of 5 modules, HDFloat for hydrodynamics, HDProp for hydrodynamic property calculation, HDMoor for mooring dynamics, AeroDyn for aerodynamics, DAFUL for multi-body dynamics with nonlinear elasticity, and interface program that connects each calculation module. A turbulent wind and regular wave load case is simulated for the 5-MW OC3-Hywind with a spar bouy platform and catenary mooring lines. The results are compared with the results of the FAST(developed by NREL). As a result, the overall system responses from WindHydro and FAST agree well although some differences in the generator responses are observed.

• The Plant Pathology Journal
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• 제40권3호
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• pp.290-298
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• 2024

K-Maryblyt has been developed for the effective control of secondary fire blight infections on blossoms and the elimination of primary inoculum sources from cankers and newly emerged shoots early in the season for both apple and pear trees. This model facilitates the precise determination of the blossom infection timing and identification of primary inoculum sources, akin to Maryblyt, predicting flower infections and the appearance of symptoms on various plant parts, including cankers, blossoms, and shoots. Nevertheless, K-Maryblyt has undergone significant improvements: Integration of Phenology Models for both apple and pear trees, Adoption of observed or predicted hourly temperatures for Epiphytic Infection Potential (EIP) calculation, incorporation of adjusted equations resulting in reduced mean error with 10.08 degree-hours (DH) for apple and 9.28 DH for pear, introduction of a relative humidity variable for pear EIP calculation, and adaptation of modified degree-day calculation methods for expected symptoms. Since the transition to a model-based control policy in 2022, the system has disseminated 158,440 messages related to blossom control and symptom prediction to farmers and professional managers in its inaugural year. Furthermore, the system has been refined to include control messages that account for the mechanism of action of pesticides distributed to farmers in specific counties, considering flower opening conditions and weather suitability for spraying. Operating as a pivotal module within the Fire Blight Forecasting Information System (FBcastS), K-Maryblyt plays a crucial role in providing essential fire blight information to farmers, professional managers, and policymakers.

• Journal of Positioning, Navigation, and Timing
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• 제13권2호
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• pp.199-206
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• 2024

The GEO-KOMPSAT-2A (GK2A) satellite, which was launched in December 2018, carries weather observation payloads and uses the image navigation and registration system to calibrate the observation images. The calibration system requires accurate orbit prediction data and depends on the accuracy of the orbit determination accuracy. In order to find a possible way to improve the current orbit determination accuracy of the GK2A flight dynamic subsystem module, orbit determination software was developed to independently evaluate the orbit determination accuracy. A comprehensive satellite dynamic model is applied for a batch-type least squares filter. When determining the orbit, thrust firing during station-keeping maneuvers and wheel-off loading maneuvers is taken into account. One month of GK2A ranging data were processed to estimate the satellite position on a daily basis. The orbit determination error was evaluated by comparing estimates during overlapping estimation intervals.

• 한국해안·해양공학회논문집
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• 제31권6호
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• pp.434-449
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• 2019

파형의 왜도, 저류 외에도 구속 모드의 외중력파, 경계층 streaming이 반영된 개선된 횡단표사 모듈이 제시되었으며, 개선된 모듈에서는 단위 관측기간 내에서 출현하기 마련인 개별 파랑도 고려된다. 이어 불규칙한 개별 파랑이 표사이송에 미치는 영향을 확인하기 위해 단조해안에서의 비선형 천수과정과 해변변형을 수치모의 하였다. 모의결과 최근 적용범위가 쇄파역으로 확대된 주파수 영역 Boussinesq Eq.은 쇄파역에서 흔히 관측되는 치근 모양의 파형, 구속 모드의 외중력파, 경계층 streaming의 모의가 가능한 것으로 판단된다. 또한 연 최대 고파랑이라는 해양환경을 등가 비선형 규칙파[Cnoidal wave]로 해석하는 경우 최대 횡단표사 이송률은 불규칙 파랑에서 관측되는 이송률의 세 배에 달할 정도로 지나치게 과다하게 모의되었으며, 이는 외빈과 원빈의 과다한 침식으로 이어졌다. 또한 연안 표사 이송과 관련된 free parameter K를 최적화하기 위해 맹방해빈의 2017.4.26부터 2018.4.20까지의 해안선 변화를 수치모의 하였으며, 최적화 과정에는 실측된 해안선 위치를 활용하였다. 모의 결과 맹방 표사계의 경우 최적화된 K는 0.17로 보이며, 이 경우 10월 말에 연이어 내습한 최대 고파랑에 의해 침식된 해안이 동절기와 춘절기의 너울에 의해 점진적으로 복원되는 대순환 과정을 거쳐 해안선이 맹방해안 남단과 북단에서는 18 m, 맹방 해안 중앙부에서는 2.4 m 내외로 전진하는 관측결과에 상당히 근접한 수치모의가 가능한 것을 확인하였다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제12권5호
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• pp.19-32
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• 2007

우리나라에서 대표적으로 사용되어 온 지하수 함양량 산정방법은 지하수 감수곡선에 의한 기저유출분리법, 유역내의 집중형 개념모형에 의한 물수지 분석, 그리고 지하수위 변동곡선법으로 대별된다. 지하수 함양량은 기후조건, 토지이용 그리고 수문지질학적 불균질성에 따르는 시공간적인 변동성을 나타내므로 전술한 방법들은 이같은 특성을 다루기에는 많은 제약을 가진다. 이 같은 단점을 극복하기 위해 본 연구에서는 통합 지표수-지하수 모형인 SWAT-MODFLOW로부터 얻어진 물수지 성분을 기초로 한 새로운 함양량 추정방법을 제시하고자 한다. 하천변에 위치한 지하수위는 하천흐름과 유사한 동적변화를 나타내는 반면 보다 상류부에 위치한 지하수위는 강수에 대하여 일정한 지체현상을 나타낸다. 이와 같은 지하수위 변동의 특징은 함양의 물리적 특성과 관련되므로 이 같은 현상을 설명하기 위해서는 토양층을 통과한 물이 대수층 함양에 도달하는데 걸리는 시간적 지체를 설명하는 것이 필요하다. SWAT 모형에서는 주어진 날에 대해 물이 토양층을 통과하여 대수층으로 함양되는 과정을 설명하기 위해 지수형태의 감쇠가중함수를 가진 단일 저수지 저류 모듈을 사용한다. 그런데 이 모듈은 지체시간이 긴 경우나 추정된 함양의 시계열이 지하수위 시계열과 잘 맞지 않는 제한 사항을 가지므로, 본 연구에서는 비포화대를 통과하는 시간 지체를 보다 현실적으로 반영할 수 있는 다단 저수지 저류 추적 모듈을 개발하여 SWAT모형에 탑재하였으며, 이 모듈내의 시간지체와 관련된 매개변수는 관측지하수위와 모의 함양량의 상관관계를 검토함으로써 최적화가 이루어지도록 하였다. 본 연구방법의 최종 단계는 모의 지하수위와 관측 지하수위, 그리고 관측 유역유출량과 모의 유출량을 검증함으로써 종결된다. 새롭게 제안된 방법을 우리나라 미호천 유역에 적용하여 지하수 함양량의 시공간적 분포를 추정하였는데 제시된 방법이 유역 모형의 효율성과 지하수위 변동법의 정확성이라는 장점을 모두 가진 방법이어서 추정된 일 함양량은 수리지질학적 불균질성, 기후조건, 토지이용과 토양층과 대수층의 거동까지 반영한 매우 개선된 값으로 판단된다.