• Journal of Korean Society of Forest Science
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• v.81 no.4
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• pp.310-319
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• 1992

Vegetational data obtained from 24 quadrats of Mt. Paekdu area were analysed by applying two multivariate methods : two-way indicator species analysis(TWIMSPAN) for classification and detrended canonical correspondence analysis(DCCA) for ordination. DCCA technique allowed to extract the ordination axes that could be related to 9 environmental factors. The dominant tree species distributed in the region between 1,700-1,950m from sea level were Betula ermanii, Larix olgensis, Abies nephrolepis, and Picea jezoensis in descending order. The dominant tree species distributed in the region between 1.300-1.500m were Abies nephrolepis, Pinus koraiensis, Larix olgensis, Betula mandshurica, and Picea jezoensis in descending order. The available phosphorus content in forest soil ranged from 7 to 1.153ppm. The distribution of the major communities showed a close relationship with elevation gradient. From high to low elevation, the communities of Alnus fruticosa var. mandshurica, Betula ermanii, Larix olgensis, Abies nephrolepis, Picea jezoensis, Pinus koraiensis, and Quercus mongolica were found in descending order. At the communities where Betula ermanii was dominant, Betula ermanii forest was distributed in the high elevation area, Betula ermanii-Larix olgensis forest in the good nutrition of organic matter and $K^+$, and Abies nephrolepis forest in the good nutrition of $Mg^{{+}{+}}$ and $Ca^{{+}{+}}$. The most dominant factor influencing community distribution was elevation.

• Mycobiology
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• v.50 no.5
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• pp.317-325
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• 2022

Endophytic fungi occupy various ecological niches, which reinforces their diversity. As few studies have investigated the endophytic fungi of alpine conifers, we focused on four species of alpine conifers in this study-Abies nephrolepis, Pinus pumila, Taxus cuspidata var. nana, and Thuja koraiensis-and examined them for endophytic fungi. A total of 108 endophytic fungi were isolated. There were four taxa in A. nephrolepis, 12 in P. pumila, 18 in T. cuspidata var. nana, and 17 in T. koraiensis; these were divided into five classes: Agaricomycetes (3.2%), Dothideomycetes (29.0%), Leotiomycetes (15.0%), Sordariomycetes (41.9%), and Orbiliomycetes (1.6%). The most prevalent fungi were Sydowia polyspora (22.7%) and Xylariaceae sp. (22.7%) in P. pumila, Phomopsis juglandina (16.1%) in T. cuspidate var. nana, and Thuja-endophytes sp. 1 (70.0%) in T. koraiensis. However, there was no dominant species growing in A. nephrolepis. Some host plants were analyzed using next-generation sequencing. We obtained 4618 reads for A. nephrolepis and 2268 reads for T. koraiensis. At the genus level, the top three endophytic fungi were Ophiostomataceae_uc (64.6%), Nectriaceae_uc (15.5%), and unclassified organism (18.0%) in A. nephrolepis and Nectriaceae_uc (41.9%), Ophiostomataceae_uc (41.8%), and Magnaporthaceae_uc (9.2%) in T. koraiensis. Our results show that there are different communities of endophytic fungi among different host plants, even if the host plants are in the same region. Such ecological niches are important in terms of the ecological restoration of alpine conifers.

• Journal of Life Science
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• v.34 no.3
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• pp.160-169
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• 2024

In this study, Abies nephrolepis MAX. was divided into A. nephrolepis MAX. stem (AS) extract and A. nephrolepis MAX. leaf (AL) extract. Their anti-inflammatory abilities and applicability as cosmetic materials were determined. Tests of the cell survival rate measured using RAW 264.7 cells and extracts of AS and AL showed 97.8% and 95.6% cell viability at a 500 ㎍/ml concentration. To determine anti-inflammatory activity, we examined the inhibitory effects on the production of LPS-induced NO in RAW 264.7 cells by Griess assay. The results showed that the AS and AL extracts presented a concentration-dependent inhibition of NO production. The protein expression inhibitory effects of AS and AL extracts were measured by western blot at 25, 50, and 100 ㎍/ml concentrations. β-actin was used as a positive control. The results of western blot of extracts from AS showed that the expression inhibition rate of the iNOS protein was decreased by 50.1% at the 100 ㎍/ml concentration. Additionally, the results of western blot of AL extracts showed that the expression inhibition rate of COX-2 and iNOS protein was decreased by 66% and 8.2% at the 100 ㎍/ml concentration. The mRNA inhibitory effect was measured by RT-PCR at 25, 50, and 100 ㎍/ml concentrations. GAPDH was used as a positive control. Consequently, the iNOS mRNA expression effect by RT-PCR of AS extract demonstrated by RT-PCR decreased by 27.9% at the 100 ㎍/ml concentration, and the iNOS and IL-6 mRNA expression effect of AL extract measured by RT-PCR decreased by 48.6% and 48.7% at the 100 ㎍/ml concentration.

• Korean Journal of Environment and Ecology
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• v.36 no.4
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• pp.413-421
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• 2022

This study was conducted to identify the vegetation structure of the Abies nephrolepis forests by analyzing species composition in Mt. Doota and Mt. Cheongok in the Gangwon region with the phytosociological method. In order to perform a field investigation, 18 survey areas with an area of about 400 m2 were set up for the stands where A. nephrolepis grow wild. Communities were classified through TWINSPAN and visualized using DCA (Detrended Correspondence Analysis). Species diversity analysis, importance value analysis, and DBH class analysis were employed to identify the characteristics of each community. As a result, the A. nephrolepis forests in Mt. Doota and Mt. Cheongok have been classified into 4 communities; GroupI, II, III and IV. GroupIis Quercus mongolica-A. nephrolepis competitive forest dominated by Q. mongolica and A. nephrolepis in the order. Group II is A. nephrolepis-Betula ermanii competitive forest dominated by A. nephrolepis, Sorbus commixta, and B. ermanii in the order. Group III is dominated by A. nephrolepis, but is expected to have competition between A. nephrolepis and Q. mongolica in the future due to the mass occurrence of Q. mongolica saplings. A. nephrolepis, S. commixta, and Q. mongolica are the dominant species in Group IV, and A. nephrolepis is the major dominant species in all layers. The species diversity of the A. nephrolepis forests in Mt. Doota and Mt. Cheongok is lower than other A. nephrolepis forests in Mt. Seorak, Maruguem, and Mt. Odae. It is necessary to establish conservation measures and to continuously monitor the A. nephrolepis forests in Mt. Doota and Mt. Cheongokis due to the harsh environment and the competition that may occur between A. nephrolepis and deciduous trees such as Q. mongolica in the future.

• Korean Journal of Agricultural Science
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• v.20 no.2
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• pp.145-152
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• 1993

Vegetational data obtained from 15 quadrats of Mt. Daeam area were analysed by applying two multivariate methods : two-way indicator species analysis(TWINSPAN) for classification and detrended canonical correspondence analysis(DCCA) for ordination. The dominant tree species were Quercus mongolica, Acer pseudo-sieboldianum, Betula costata, and Acer mono in descending order. The pH ranges were 5.0 to 5.5 in the 15 sites near bog and 5.3 to 5.4 in the 3 sites within bog. The forest communities in Mt. Daeam were classified into Quercus mongolica-Acer mono, Quercus mongolica-Prunus sargentii, Quercus mongolica-Abies nephrolepis-Acer tschonoskii var, rubripes, and Quercus mongolica-Abies nephrolepis-Betula costata according to the TWINSPAN. The relationships between the distribution of dominant species of forest vegetation and soil in the forest of Mt. Daeam were investigated by analyzing soil nutrition gradient. Quercus mongolica-Prunus sargentii forests were distributed in the good sites in nutrition levels of total nitrogen, cation exchange capacity, $Mg^{+{+}}$ and $Ca^{+{+}}$ but Quercus mongolica-Abies nephrolepis-Betula costata forests in the poor sites in the nutrition levels.

• Journal of Korean Society of Forest Science
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• v.100 no.4
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• pp.577-584
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• 2011

To estimate level of genetic variation and genetic differentiation among populations of 3 populations in Abies koreana and 5 populations in Abies nephrolepis, 5 nSSR markers were analyzed. Except 1 locus where too many alleles were observed excessively, population genetic parameters were recalculated with 4 loci. Mean expected heterozygosities ($H_e$) were 0.292 in A. koreana and 0.220 in A. nephrolepis, respectively. In both species, positive fixation coefficient was estimated (F=0.065 for A. koreana and F=0.095 for A. nephrolepis), which suggests that there is an excess of homozygotes relative to Hardy-Weinberg expectations within populations. Relatively high degree of population differentiation was observed in A. koreana ($F_{ST}=0.063$). compared to that of A. nephrolepis ($F_{ST}=0.039$). From 3-level Hierarchical estimation of F-staticstics, only 4.9% of the genetic variation was allocated between species ($F_{PT}$), which suggested that most of genetic variation was shared between two species. On the basis of results from analysis of genetic relationships among populations, 2 populations of A. koreana (Mt. Halla and Mt. Deogyu) were genetically distinct from the populations of A. nephrolepis but a population of Mt. Jiri was allocated within a group of populations of A. nephrolepis. Populations of both species seemed to have undergone genetic drift due to gradual decrease in population size induced by global warming after the last glacier, which resulted in increase of homozygotes by inbreeding. It could be also postulated that these species might be diverged recently and It is likely that the two species have not fully speciated yet.

• Journal of Environmental Impact Assessment
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• v.15 no.5
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• pp.323-337
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• 2006

This work discussed the species composition, phylogeny, spatio-temporal distribution, ecology and natural history of North Korean Pinaceae or pine tree family, which seems to be important to maintain nature and ecosystem in the Korean Peninsula. Out of five genera and sixteen species of Pinaceae of the Korean Peninsula, North Korea contains four genera and eleven species of Pinaceae, including Pinus densilflora, P. koraiensis, P. pumila, Picea jezoensis, P. koraiensis, P. koraiensis var. koraiensis, P. pungsanensis, Larix gmelini, L. gmelinii var. olgensis, Abies holophylla and A. nephrolepis. In terms of phylogeny Pinus is closely related to Picea, and followed by Larix. Abies is close to Tsuga which only occur at Ullung Island. Distributional pattern of North Korean Pinaceae can be classified into four types; three species of nation-wide montane type i.e., Pinus densilflora, P. koraiensis and Abies holophylla, four species of central and northern subalpine type, i.e., Pinus pumila, Picea koraiensis, Larix gmelini and Abies nephrolepis, one nation-wide subalpine type, Picea jezoensis, and three species disjunctive to north type, i.e., Picea koraiensis var. koraiensis, P. pungsanensis, and Larix gmelinii var. olgensis. Pinaceae species occurring on the alpine and subalpine belts of North Korea, such as Pinus koraiensis, P. pumila, Picea jezoensis, P. koraiensis, P. koraiensis var. koraiensis, P. pungsanensis, Larix gmelini, L. gmelinii var. olgensis and A. nephrolepis are considered as the glacial descendant from the boreal region. Those species might have migrated from the north during the Pleistocene glacial epochs in search of favourable condition, and since the Holocene period they survived on the hostile alpine and subalpine environments, in which they are more competitive than warmth-tolerant temperate vegetation. Certain species, such as Picea pungsanensis, is segregated on the isolated mountains since the Pleistocene period, and forced to adapt to local environment, and eventually became an endemic species of North Korea. Recent rapid global warming trend especially in northern high mountains of North Korea could cause an unfavourable environment for the survival of cold-tolerant Pinaceae of the alpine and subalpine belts. Pinus densiflora, which is occurring on the montane belt might faced with difficulties due to both the deforestation and the outbreak of insect-borne disease, such as Bursaphelenchus xylophilus.

• Korean Journal of Environment and Ecology
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• v.35 no.1
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• pp.37-47
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• 2021

This study carried out the plant sociological survey of Abies nephrolepis forest in Mt. Seorak, which is in danger of deterioration due to the accelerated climate change. We examined seventy quadrats obtained from the survey and used the TWINSPAN technique to classify communities. We then performed the DCA method for the sequence analysis and analyzed the characteristics of each community. A. nephrolepis forest of Mt. Seorak is composed of four communities (A. nephrolepis-Lonicera caerulea var. edulis community, A. nephrolepis-Acer komarovii community, A. nephrolepis-Ac. pseudosieboldianum community, and A. nephrolepis-Betula costata community). Each community showed a different distribution according to location because different microenvironments are formed depending on location such as altitude and slope direction, resulting in different species composition. Each community showed differences in environmental characteristics such as altitude, rock ratio, soil characteristics, and litter layer thickness. As a result, there were significant differences between communities in the number of species and individuals, coverage, tree size, and species diversity, as well as differences in species composition. The A. nephrolepis-L. caerulea var. edulis and A. nephrolepis-Ac. komarovii communities were located in high altitude with high rock ratios and had little development of tree layer. On the other hand, the A. nephrolepis-Ac. pseudosieboldianum and A. nephrolepis-B. costata communities were relatively in low altitude with high soil ratio and had the development of tree layer with high species diversity.

• The Korean Journal of Ecology
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• v.22 no.3
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• pp.119-129
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• 1999

The bryophyte communities on the forest floor of the mixed coniferous and deciduous broad-leaf forest and the evergreen coniferous forest of cool temperate and subalpine zones in Mts. Taebaek, Odae and Sulak of Tabaek Range were investigated from. the numerical and phytosociological viewpoints. As a result, four bryophyte communities were recognized there, considering the species composition of vascular vegetation; A. Thuja koraiensis-Abies nephrolepis/Taxus caespitosa-Pinus pumila/Hylocomium splendens-Pleurozium schreberi community, B: Sasamorpha borealis-Abies nephrolepis/Plagiomnium cuspidatum- Aulacomnium heterostichum community, C. Abies holophylla-Abies nephrolepis/Thuidium recognitum-Trachycystis immarginata cristatus community, D. Meehania urticifolia-Abies nephrolepis/Hylocomiopsis ovicarpa-Taxiphyllum aomoriense community. Among these, the community A was distributed in higher altitudes and characterized by the species group of the holarctic elements, e.g. Hylocomium splendens, Pleurozium schreberi, Dicranum majus, etc. limited in subarctic; subalpine and arctic; alpine zones and was confirmed as synusies of the Thujo-Abietetum nephrolepidis Song 1991 and Taxo-Pinetum pumilae Song 1985 belonging the Vaccinio-Piceetea Br.-Bl. 1939. In contrast the communities B, C and D were distributed in the mixed coniferous and deciduous broad-leaf forest of lower altitudes and characterized by the elements of the cool temperate; montane zone. They were confirmed as synusies belonging to the Corylo-Quercetum mongolicae Song 1988. By the result of the numerical syntaxonomical analysis such as cluster analysis, interspecific association, PCA, the communities were largely divided into the two types. The two types coincided with the community units or the diagnostic species of the bryophyte communities of the subalpine zone and the montane zone, respectively. Also the analysis revealed that the division of the above two forest zones are justifiable. Up to date many investigator have described the coniferous forest distibuted above 1000m in Taebaek mountains as the forest vegetation of subalpine zone. However, from the results of the present study, the boundary of the mixed forest and the true subalpine coniferous forest was elucidated to be ca. 1,500 m phytosociologically. Species richness was the highest in the community A, and the other four communities showed a similar species richness.

• Korean Journal of Environment and Ecology
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• v.5 no.1
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• pp.32-41
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• 1991

A survey of subalpine zone (altitude is 1,360-1,410m) in Mt. Chiri, was conducted using 25 sample plots of 500$m^2$ size. The classification by TWINSPAN and DCA ordination were applied to the study area in order to classify them into several groups based on woody plants and environmental variables. By TWINSPAN techniques, the plant community were divided into five groups by importance value of Abies nephrolepis. The dividing groups are Fraxinus rhynchophylla - Magnolia sieboldii community, Quercus mongolica - Rhododendron schlippenbachii community, Q. mongolica - F. rhynchophylla - Acer pseudo-sieboldianum community, Q. mongolica- A. nephrolepis- Rh. schlippenbachii community, and A. nephrolepis- Pinus densiflora - Rh. schlippenbachii community. The successional trends of tree species by both techniques seem to be from Q. mongolica to A. nephrolepis in the canopy layer. It was no difference between the stand scores of DCA and environmental variables.