• Journal of Aquaculture
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• v.4 no.1
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• pp.31-66
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• 1991

This paper deals with the reproductive ecology e.g., number of the pre-spawning moults, morphological characteristics of the pre-spawning moult the common moult, daily ration druing a molting cycle mating behavior, structures of spermatozoa and spermatophore, structure of vas deferens, mechanisms of the oviposition and brooding into the egg-chambers, a suitable time for the artificial mating and fertilization, time sequence of the oviposition and brooding into egg-chambers from the copulation, responses to temperature and chlorinity on the egg development and hatching, effect of temperatures on duration of egg development, physical mechanism of the egg hatching, to make an attempt for the artificial spawning and brooding to establish a suitable system of the artificial seedling-production for the aquaculture. 1. Females molted commonly $8{\~}10$ times at an interval of $17{\~}18$ days at $28^{\circ}C,\;3.26\~4.35\%_{\circ}$ while the prespawning moltings were $4{\~}5$ times at an interval of $13{\~}14$ days. The suitable state for artificial copulation was within 14 hours elapsed from the prespawning moltings (most suitable state was within 8 hours). Males discharged a gelatinous spermatophore and placed it on the females sternum during copulation. Oviposition was seen $6{\~}17$ hours after copulation. External fertilization was considered to take place at oviposition. Fertilized eggs held in egg-chambers forming between pleopods were about $5000{\~}6000$ in females those sizes about 6.5 cm in body length. 2. Eggs immediately after oviposition were elliptic shape, measuring $0.58{\times}0.48$ mm up to hatching. Their sizes increased with egg development and finally reached $0.85{\times}0.54$ mm up to hatching. The relationship between the long axis of the egg(Y in U) and days elapsed(X) was expressed as Y= 5.60194 + 0.007358X. The eggs performed superficial cleavage and their cleavage furrows became visible at the 4-daughter-nucleus stage. The eggs showed normal development up to hatching at water temperature range of $22{\~}30^{\circ}C$ (optimum temperature : $26{\~}28^{\circ}C$) and at chlorinity range of $0.00\~6.64\%_{\circ}$ (optimun chlorinity : $2.21{\%}_{\circ}$). The relationship between incubation period (Y in days) and water temperature(X in $^{\circ}C$) could be expressed as Y= 50.803-1.3555X. The eggs hatched $12{\~}13$ days after oviposition at $28.0{\~}28.6^{\circ}C$ 3. The pre-spawning moltings were appreciably different in the morphologic structure from those of common moltings. Breeding setae and dresses were formed on the thoracic regions, abdominal epimerae and the bases of the first to fourth pleopods in order to prepare and support oviposition, transfering and supporting eggs in egg-chambers up to hatching. These supplementary breeding organs were observed only at reproductive seasons.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.7 no.1
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• pp.1-7
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• 2004

Purpose: Alvarado scoring system was evaluated regarding its usefulness for the early diagnosis of acute appendicitis in adult and in reduction of the incidence of negative appendicectomies. To evaluate the accuracy of diagnosing appendicitis using the Alvarado score in children. Methods: Prospectively, we surveyed 122 patients (male 67, female 55) suffering from abdominal pain, who had visited to the emergency department of Chosun University Hospital from June 2002 to May 2003. The Alvarado score has been computed from the white blood cell count, neutrophil count, body temperature, resistance in the right lower quadrant, length of symptoms, nausea and vomiting. Each patient was evaluated by a pediatric resident and then by a general surgeon independently. Results: Out of 170 total children who visited to the emergency department due to abdominal pain, 122 patients were associated with appendicitis. A total of 122 patients (67 male and 55 female) were visited to the emergency room with suspected appendicitis. From 105 operated patients, 92 (87.6%) were diagnosed acute appendicitis and erronous diagnostic rate was 12.4%, pathologically. Mean alvarado score of appendicitis group was $5.40{\pm}1.24$ whereas those of non-appendicitis group was $3.73{\pm}1.82$ (p<0.05). From 6 Alvarado score high sensitivity (86.4%) and high specificity (80.0%) were observed. Sensitivity of ultrasonography or computed tomography was 92.5%. Conclusion: We found that Alvarado score system is a noninvasive, safe diagnostic method, which is simple, reliable and repeatable. Alvarado score is useful system for a first, rapid and economic evaluation for the appendicitis in children.

• Korean journal of applied entomology
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• v.52 no.2
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• pp.93-100
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• 2013

In this study, the distribution of Cantao ocellatus in Korea occurred at Jindo, Tongyoung, Gwangju and Taean including Jejudo in 2012 and was coincidentally in accordance with the distribution of Mallotus japonicus as a host plant. The adult emerges in M. japonicus occur from late June to early July and can be observed until the end of October. The investigation of the female and male was measured, developmental characteristics, host plant and oviposition preference under the conditions of temperature $25^{\circ}C({\pm}2)$, humidity 65%(${\pm}2$), day length 16L:8D, by indoor breeding. For females and males respectively, average body lengths were 26.20 mm and 23.88 mm, body widths 11.35 mm and 10.57 mm, head widths 3.84 mm and 3.64 mm, probosics lengths 7.90 mm and 7.27 mm, antennal lengths 9.87 mm and 9.69 mm, anterior leg lengths 12.50 mm and 12.27 mm, intermediate leg lengths 14.61 mm and 13.12 mm, posterior leg lengths 16.90 mm and 16.53 mm, and fresh weights 0.46 g and 0.31 g. It was seen that two kinds of C. ocellatus had prickles at the end of the pronotum which had developed in the fifth instars. The female is distinguished from the male by the reproductive organ and the spotted pattern on the abdominal segment. The preference of drinks was fruit, leafstalk, midrib, and branch in Mallotus japonicus (Thunb.) Muell. Arg., Mallotus japonicus 'Variegatus', Ricinus communis L., Lonicera japonicus var. repens (Siebold) Rehder, Citrus sinensis (L.) Osbeck and Zelkova serrata (Thunb.) Makino.

• Journal of Acupuncture Research
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• v.23 no.4
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• pp.1-14
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• 2006

Objectives : We implemented the Moxa-Extract Moxibustion Method in order to improve the conventional moxibustion therapy. This method is aimed to eliminate burning wounds and smoke, which are the defects of conventional moxibustion therapy. And we performed to verify the efficiency by comparing the Moxa-Extract Moxibustion Method with the conventional Indirect Moxibustion Method. We measured the body heat and the lasting time of blood circulation improveent using thermography. We implemented the Moxa-Extract Moxibustion Method in order to improve the conventional moxibustion therapy. This method is aimed to eliminate burning wounds and smoke, which are the defects of conventional moxibustion therapy. And we performed to verify the efficiency by comparing the Moxa-Extract Moxibustion Method with the conventional Indirect Moxibustion Method. We measured the body heat and the lasting time of blood circulation improvement using thermography. Methods : The moxibustion therapy has two kinds of effects: The formers are pharmacological effects of the Moxa's vasodilators and antioxidants. The latters are thermal effects which cause improvement of the blood circulation. To remove the demerits without omission of above therapeutic effects, we extracted the vasodilators and antioxidant compounds from the Moxa-CH2Cl2 fraction Moxa-EtOA and composed the moxibustion kit with (Ba0.8 Sr0.2)0.996 Y0.004 TiO2 + 0.5WT SiO2% positive Temperature Coefficients Thermistor. The experimental demonstrations have been made by the stimulating the spot which is CV4(Kwan-Won), CV8(Shin-Guel), CV12(Jung-Wan) acupuncture points of the conception vessel meridian(CV). And stimulating time was one hour. We divided the subjects into 5 groups such as no stimulation group, conventional Indirect Moxibustion group, only Moxa-Extract stimulation group, only heat stimulation group, and Moxa-Extract Moxibustion group. In the different cases, we have measured the body heat in pre-stimulation, just after stimulation, 2 hours after, and 4 hours after. Results : he body heats of the group who were stimulated by the Moxa-Extract Moxibustion method were increased by over the $2^{\circ}C$. And the body heats of the group who were stimulated by the Indirect Moxibustion Method were increased by average the $1^{\circ}C$. We have evaluated that the Moxa-Extract Moxibustion Method is improvement on the Indirect Moxibustion Method by the increasing rate is 200% and increasing time is 150% with the body heat of the abdominal region. Conclusion : We have implemented the Moxa-Extract Moxibustion Method and evaluated the efficiency of the Moxa-Extract Moxibustion Method comparing with the Indirect Moxibustion Method.

• Journal of Life Science
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• v.28 no.9
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• pp.1118-1126
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• 2018

In this study, investigated the general characteristics and diagnostic methods types of streptococcosis among various fish disease pathogens that caused a lot of economic damaged to aquaculture fish based on the previous research paper. Streptococcosis infection of fish is considered a reemerging disease affecting a variety of wild and cultured fish throughout the world. Calssifiacation of Gram positive cocci based on DNA-DNA hybridization coupled with 16S sequencing has shown that at least five different species are considered of significance as fish pathogens: Lactococcus garvieae, L. piscium, Streptococcus iniae, S. agalactiae, S. paruberis, Vagococcus salmoninarum. Symptoms of infection with streptococcosis disease such as body color change, eyeball abnormality, gill discoloration, bleeding, abdominal distension, swelling of the kidney and spleen. In addition, it usually occurs from June to October when the water temperature rise a lot of fish death. Currently, 16S rRNA, 16S-23S rRNA intergenic spacer region (ISR), Random Amplified polymorphic DNA (RAPD), Ribotyion (RT), Loop-mediated isothermal amplification (LAMP) are among the methods for diagnosing streptococcosis. Among them, the LAMP method, which is high applicable to the aquaculture farm has attracted the spotlight, but due to problems such as confirmation of results. This seems to minimize the economic loss of streptococcosis which complements the problem so that it can be easily used from the diagnosis to the results confirmation.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.5
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• pp.509-519
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• 2013

Through the study on judgment of Body form and settle Energy flow(立形定氣) before diagnose the patients, the results are as follows. The observation of the body form is to determine prosperity and deficiency of each internal organ. It is necessary to distinguish Body form loss(形脫) and Body form fullness(形充). Fat man(肥人), Thin man(瘦人), Creamy man(膏人), Muscular man(肉人), Small Fat man(脂人) are discriminated by fat distribution, fat content, and muscle mass. The observation of the body form means the observation of structure disorder, color change, develop part at body, head and face. The observation of the body form that is to determine prosperity and deficiency of each internal organ is from the limited knowledge of the anatomy. The observation of face color is considered by blood perfusion, blood oxygenation and accumulation of carotinoid, bilirubin and change of melanin in the facial skin. The prosperity and the deficiency of energy flow is considered by symptom combined with growth (<40 years) and aging (>40 years). The prosperity of energy flow includes the anger, anxious emotion and the deficiency of energy flow includes the fear, depressive emotion. The breathing type is expiratory exhalation like asthma patients in the prosperity of energy flow. The deficiency of energy flow is weakness to overcome the disease. The prosperity and the deficiency of energy flow are considered by body metabolic ratios (Basal metabolic Rate: BMR, Resting metabolic rate: RMR, Physical activity ratios: PASs). Development of subcutaneous fat is good in the person of prosperous energy flow. The person of prosperous energy flow is hard to overcome to heat weather than cold weather. The person of deficiency of energy flow has tendencies of low blood pressure, insufficiency of blood flow in the peripheral and being shocked. The person of deficiency of energy flow has tendencies of chronic fatigue syndrome or automatic nerve disorder. If the patient who has deficiency of energy flow has severe weight loss should be checked for the presence of disease. The observation of small and large of bone is to check the development and disorder of bone growth and aging. The observation of thickness and weakness of muscle is to check the development of muscle, particularly biceps, gastrocnemius, and rectus abdominal muscle. The observation of thickness and weakness of skin is to check the ability of regulating body temperature by sweating.

• Korean Journal of Veterinary Research
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• v.47 no.3
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• pp.291-298
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• 2007

Olive flounder, Paralichthys olivaceus is one of the most important cultured fish in Korea, its farming has been negatively impacted by viral, bacterial and parasitic diseases. Streptococcal infection was considered as a serious problem because of significant economic losses in olive flounder farm industry. The development and evaluation of vaccine for protection against infection by this agent were required. We evaluated the safety and efficacy of ${\beta}$-hemolytic Streptococcus (S.) iniae vaccine on olive flounder Three hundreds of flounders (weight $119.8{\pm}20.7g$, body length $22.6{\pm}1.4cm$) were reared in 0.5 tons aquaria in land-marine tank system. Seawater was provided from the sea of Inchon in Korea, and water temperature was set to $22^{\circ}C$ and $25^{\circ}C$ in the vaccination and challenge test, respectively. We used the formalin-inactivated ${\beta}$-hemolytic S. iniae (F2K) vaccine (M VAC INIAE; Kyoritsu seiyaku, Japan) originated in Japan. The vaccine was intraperitoneally administered to fish. Both of vaccinated group and control group were challenged with intraperitoneally injection by virulent S. iniae SI-36 isolates with $1.0{\times}10^7CFU/fish$ at 3 weeks after vaccination. Difference on mortality of control and vaccinated group (90.0 and 15.0%, 76.5 and 8.0% respectively) at two trials were found significant (p<0.05), and relative percent survival were 83.4% and 89.5%, respectively. The dead fishes were showed dark pigmentation of skin, abdominal extension, hemorrhagic ascites, and liver necrosis, and isolated the S. iniae strain from ascites, liver and kidney. We confirmed the safety and efficacy of ${\beta}$-hemolytic S. iniae vaccine by determinations of the optimal management condition and artificial challenge test in olive flounder.

• Korean Journal of Food Science and Technology
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• v.18 no.6
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• pp.485-491
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• 1986

Gulbi were made by salting fresh Yellow corvenia (Pseudosciaena manchurica) in three ways; the dry salting method with bay-salt, the dry salting method with purified salt or the abdominal brine injection method with purified salt. Half of the sample was dried by controlling temperature and relative humidity and the other part was dried under the natural condition. The moisture content of the samples were decreased more rapidly by the controlled system than by the natural condition. The lipid content and the iodine values of the muscle and skin of the Gulbi were decreased slowly with laps of drying period. The peroxide values of the sample were increased to its peak after 10 days of drying, and were decreased rapidly thereafter. Both acid values and the thiobarbituric acid values were increased. The deterioration of lipids during Gulbi processing was not notable depending on the salting method, but the natural drying condition affected more severely in their deterioration.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.22 no.3
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• pp.129-137
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• 1989

Sand fish, Arctoscopus japonicus (Steindachner) is distributed in the coastal waters of East Sea of Korea, Japan, and Alaska. On December 1, 1987, matured adult of sand fish were collected from the shore of Ok-kye, Myongju-gun, Kangwon-do, Korea. The authors carried out artificial insemination on boat. The fertilized eggs were incubated and the larvae were reared in laborato교. The eggs of this species were demersal and adhesive, and their diameter were varied within $3.1\~3.4$mm (mean 3.3 mm, n= 10). They have a number of small oil globules. The spawned eggs in nature were formed the egg mass which were measured ca. 4 m in dia-meter. The hatching took place in 65 days after fertilization at the water temperature of $8.7\~12.3^{\circ}C$. The newly hatched larvae were $8.5\~10.2$ mm in total length with 11 (abdominal) +40 (caudal) = 51 myomeres. 24 days after hatching, the larva attained 19.4 mm in total length, at this time the larvae absorbed the yolk completely, and become postlarvae. 32 days after hatching, the larva attained 23.4 mm in total length, and become juvenile. 56 days after hatching, the juvenile reached 29.9 mm in total length and had adult form. 5 spines of preopercle bone were formed at 24.4 mm in total length. At ca. 15 mm in total length, the form of the pectoral fin was transformed into the adult form.

• Journal of Aquaculture
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• v.11 no.1
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• pp.49-58
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• 1998

The spawning behavior and early life history of the Korean aucha perch, Coreoperca herzi were studied. The eggs were spawned in a one-layer mass, hanging from the underside of a largy stone and guarded by one male. According to the considerable size difference os eggs in the individual ovaries, it was concluded that they spawns several times within a spawning season of May~June. The ripe eggs were demersal and adhesive, and their diameter were 2.60~3.04mm with several oil globule of 0.10~0.64mm. Hatching in the tank with $19.0{\pm}1.0^{\circ}C.$ in men water temperature started from the 380 hours after fertilization. Newly-hatched larvae were measured 6.45~7.34mm in total length (TL, mean : 6.85mm), and mouth and anus were open. Melanophores were present on the head, around the yolk, and on the dorsal part of the abdominal region. 3 days old larvae were measured 7.00~7.40mm in TL (mean : 7.25mm), and they fed Daphnia sp. and Brachionus plicatilis actively. 6 days old larvae transformed to postlarval stage and they were 7.94~10.10mm in TL (mean : 9.00mm). As yolk sac were nearly absorbed, the caudal notochord flex at 45. Aggregate numbers of all fin rays were completed at over 16.90~19.80mm in TL (30 days after hatching), at which time the larvae reached the juvenile stage. In fifty days old larvae(24.60~27.10mm in TL) were similar in body form, lateral line and color to adult.