• Journal of Plant Biology
• /
• 제37권2호
• /
• pp.151-158
• /
• 1994

Aleurone layers of normal and vp1 mutant maize kernels were extracted and centrifuged at 100,000g to yield a cytosol fraction. Binding of [3H]ABA cis, trans (＋)ABA to a soluble macromolecular components present in the cytosol was demonstrated by Sephadex chromatography and non-denaturing PAGE. The binding component was of high molecular weight and seems to be an aggregate of proteins. A rapid DEAE-cellulose filter method for assaying bound [3H]ABA to a soluble protein was adapted. Binding assays were performed with cytosol that had been preheated or incubated with several enzymes, indicating that heat and protease treatments disrupted the binding. This suggested that binding occurred to proteins. Some properties of the ABA binding proteins were described. The [3H]ABA binding were reduced dramatically when unlabeled ABA was added as a competitor, suggesting a specific binding of [3H]ABA. Gel filtration profiles and autoradiogram of [3H]ABA binding showed no difference in the binding components of Vp1 and vp1/vp1 mutant cytosol, indicating that Vp1 protein is not a sole ABA binding protein.

• Korean Journal of Environmental Biology
• /
• 제23권1호
• /
• pp.47-51
• /
• 2005

Three-weeks old Commelina communis was transferred to Hoagland solution (Control, 100 μM Cd/sup 2+/, 100 μM Cd/sup 2+/ + 100 μM ABA, 100 μM Cd/sup 2+/+50 mM KCl) and grown for a week in the solution and then a number of physiological activities were investigated. In cases of Cd/sup 2+/ and Cd/sup 2+/ + ABA treatments, the growth of the plants was inhibited to 71 % and 81 %, respectively, when compared with the control, but there were no significant difference of plant growth between Cd/sup 2+/ and Cd/sup 2+/ + KCl- treated plants. In the treatments of Cd/sup 2+/, Cd/sup 2+/ + ABA and Cd/sup 2+/ + KCl total chlorophyll contents were reduced to 32%, 41% and 29%, respectively. In chlorophyll fluorescence experiments, Fv/Fm ratios were also reduced to 14∼20% and about 23%, respectively, according to the light intensity by Cd/sup 2+/ and Cd/sup 2+/+ABA-treated plants. Water stresses were increased by the treatment of Cd/sup 2+/, Cd/sup 2+/ + ABA and Cd/sup 2+/ + KCI. In Cd/sup 2+/ accumulation experiments Cd/sup 2+/ transport into the plant by ABA was not affected, but the accumulation of Cd/sup 2+/ into the roots was elevated to 13% when compared with the control. Cd/sup 2+/ transport into the root was markedly inhibited to 60% by KCl. Therefore, it could be concluded that ABA did not reduce the toxicities of Cd/sup 2+/, but enhanced Cd/sup 2+/ - induced toxicities and KCl showed no effect on Cd/sup 2+/ - induced toxicities.

• Analytical Science and Technology
• /
• 제14권5호
• /
• pp.458-463
• /
• 2001

The concentration of free PAs in the cells was decreased gradually from $500{\mu}mole$ to $290{\mu}mole$ for PUT, from $153{\mu}mole$ to $79{\mu}mole$ for SPD, from $69{\mu}mole$ to $20{\mu}mole$ for SPM during the ABA treatment within 1 hour, and was slightly changed with the increasing or decreasing between $290{\mu}mole{\sim}220{\mu}mole$ in the constant content level of PUT during the continuously ABA treatment of 2 days in the young cotyledons. The concentration of free PUT was gradually decreased from $160{\mu}mole$ to $9{\mu}mole$ during the ABA treatment within 1 hour like as cotyledons, and decreased from $9{\mu}mole$ to $5{\mu}mole$ during the continuously ABA treatment 2 days in the young hypocotyls. PUT and other PA were existed $5{\mu}mole$ during the continuously ABA treatment of 2 days in the hypocotyls. It showed that during the ABA treatment was decreased all PA concentration in the cells and PAs were not concerned directly to stress, but might regulated physiological change against stress.

• Journal of Plant Biotechnology
• /
• 제45권4호
• /
• pp.306-314
• /
• 2018

Korean ginseng (Panax ginseng) has long been cultivated as an important economic medicinal plant. Owing to the seasonal and long-term agricultural cultivation methods of Korean ginseng, they are always vulnerable to various environmental stress conditions. ABSCISIC ACID (ABA) is an essential plant hormone associated with seed development and diverse abiotic stress responses including drought, cold and salinity stress. By modulating ABA responses, plants can regulate their immune responses and growth patterns to increase their ability to tolerate stress. With recent advances in genome sequencing technology, we first reported the functional features of genes related to canonical ABA signaling pathway in P. ginseng genome. Based on the protein sequences and functional genomic analysis of Arabidopsis thaliana, the ABA related genes were successfully identified. Our functional genomic characterizations clearly showed that the ABA signaling related genes consisting the ABA receptor proteins (PgPYLs), kinase family (PgSnRKs) and transcription factors (PgABFs, PgABI3s and PgABI5s) were evolutionary conserved in the P. ginseng genome. We confirmed that overexpressing ABA related genes of P. ginseng completely restored the ABA responses and stress tolerance in ABA defective Arabidopsis mutants. Finally, tissue and age specific spatio-temporal expression patterns of the identified ABA-related genes in P. ginseng tissues were also classified using various available RNA sequencing data. This study provides ABA signal transduction related genes and their functional genomic information related to the growth and development of Korean ginseng. Additionally, the results of this study could be useful in the breeding or artificial selection of ginseng which is resistant to various stresses.

• Applied Biological Chemistry
• /
• 제21권2호
• /
• pp.84-102
• /
• 1978

Barley embryoless half seeds were incubated in medium containing $10{\mu}M$ GA. Time course activity changes of ${\alpha}-amylase$ were studied in extract and medium seperately by the addition of $0.1{\mu}M,\;5{\mu}M,\;and\;10{\mu}M$ ABA in midcourse incubation of 10 hours after GA treatment. MAK profiles of nucleic acids in embryoless half seeds were compared either with $10{\mu}M$ GA treatment or concomitant treatment with $10{\mu}M$ GA and $10{\mu}M$ ABA after 10 hours incubation, Time course changes of weight increase, chlorophyll, protein and RNA consent in addition to RNase activity were studied in the presence of $10{\mu}M$ GA or $10{\mu}M$ ABA in barley coleoptile sections. After 20 hours incubation in the presence of plant hormones, MAK profiles of nucleic acids and reactive distribution of polysome and monosome were investigated. The above results were summarized as follows. 1) The production of ${\alpha}-amylase$ by treatment with GA alone increased at a linear rate in the incubation period and the active secretion of ${\alpha}-amylase$ began from 18 hours incubation in embryoless half seeds. 2) On the contrary to the partial inhibition by addition of $0.1{\mu}M$ ABA, the production of ${\alpha}-amylase$ was completely inhibited by both $5{\mu}M$ and $10{\mu}M$ ABA within 4 hours. Regardless of concentration of GA, the addition of $5{\mu}M$ ABA in midcourse completely inhibited the production of ${\alpha}-amylase$ 3) ABA treatment gave no effect on the secretion of ${\alpha}-amylase$. 4) There were no differences in RNA fractions between GA treatment and concomitant treatment with GA and ABA in the barlye embryoless half seeds. 5) While GA treatment increased the r-RNA fraction, ABA treatment decreased it and increased the s-RNA fraction in the coleoptile sections. 6) GA treatment increased RNA-DNA fraction best ABA treatment decreased it in the coleoptile sections. 7) While GA treatment suppressed RNase activity, ABA treatment increased it in the coleoptile sections. 8) GA treatment gave no great effect on the total RNA but ABA treatment remarkably diminished it in the coleoptile sections. 9) While GA treatment increased the growth and chlorophyll content, ABA treatment decreased them in the coleoptile sections. 10) GA treatment increased the protein synthesis and polysome formation but ABA treatment decreased them in the coleoptile sections. 11) The inhibition effect of ABA on polysome formation seemed to be resulted from the inhibition of r-RNA synthesis by ABA.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• 제63권1호
• /
• pp.25-34
• /
• 2018

This study was conducted to investigate the germination and proteome profile characteristics of wheat seeds treated under various concentrations of abscisic acid (ABA). After-ripening, the seeds of three wheat cultivars (Baegjoong, Keumkang, and Uri) showing different levels of dormancy were used. Germination index and germination rate of the cultivars was higher than 0.95% and 98%, respectively, and these were not significantly different under 0, 10, 30, and $50{\mu}M$ ABA at 7 d after germination. However, the growth of the shoot and radicle was significantly inhibited at 10, 30, and $50{\mu}M$ ABA compared to that at $0{\mu}M$ ABA. Mean ABA content of the embryos of seeds germinated at 0 and $50{\mu}M$ ABA for 7 d was 0.8 and $269.0ngmg^{-1}DW$, respectively. Proteins extracted from embryos germinated for 4 d were analyzed by two-dimensional gel electrophoresis, and proteins showing a difference of 1.5-fold or greater in their spot volume relative to that of $0{\mu}M$ ABA were identified. The expression of four protein spots increased at $50{\mu}M$ ABA and two protein spots were detected only at $50{\mu}M$ ABA; these six proteins were all identified as globulin types. Conversely, the expression of three protein spots decreased at $50{\mu}M$ ABA and were identified as cytosolic glutamine sysnthetase, isocitrate dehydrogenase, and S-adenosylmethionine synthetase 2. In conclusion, ABA did not inhibit the germination rate regardless of pre-harvest sprouting characteristics of the cultivars. However, the growth of the shoot and radicle was significantly inhibited by ABA, most likely through the down regulation of glutamine, methyl group donor, and polyamines biosynthesis, among others, while accompanied by globulin accumulation in the embryos.

• Analytical Science and Technology
• /
• 제12권5호
• /
• pp.441-446
• /
• 1999

The changes of amino acids in the cotyledons of spring - and winter radishes were analysed during the abscisic acid (ABA). ABA plus cycloheximide (CH) and ABA plus polyamine (PA) treatment. The total contents of amino acids were increased in spring radishes, and decreased in winter radishes by ABA. The contents of hydrophilic amino acids, proline, glycine, serine and cysteine in spring radishes (expecially proline), and of cysteine, leucine and phenylalanine in winter radishes (expecially cysteine and leucine)were increased, CH treatment resulted in the accumulation of amino acids by the inhibition of new synthesized protein, which synthesized against ABA induced dehydration both of radishes. On the contrary, the contents of amino acids were decreased in spring radishes, and increased in winter radishes during PA treatment. These results indicated that the accumulated hydrophilic amino acids and new synthesized proteins induced the adaptation of dehydration against stress and the role of ABA in accelerating of stress adaptation was mediated by polyamines.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• 제47권3호
• /
• pp.174-185
• /
• 2002

This study was carried out to investigate the effects or abscisic acid (ABA), three concentrations, on growth, activities of antioxidant-related enzymes and grain yield of two rice plants (Oryza sativa L. cv. Hwamyungbyeo and cv. Namchunbyeo) exposed to ozone (0.150 ppm) for 6 hours a day for 30 days. The leaf chlorop0hyll contents, plant height, and tillering numbers were not shown significant difference. But ABA 10$^{-5}$ M treatment affects to growth slightly. In all concentrations of ABA, superoxide dismutase (SOB) activities were increased at tillering stage of Namchunbyeo which was exposed to ozone. It is considered that the optimum concentration of ABA is 10$^{-5}$ M for minimizing loss of grain yield loss.

• Journal of Life Science
• /
• 제22권11호
• /
• pp.1515-1522
• /
• 2012

The phytohormone abscisic acid (ABA) plays an important role in the adaptive response of plants to abiotic stresses. ABA also regulates many important processes, including seed dormancy, germination, inhibition of cell division, and stomatal closure. OsABF2 (Oryza sativa ABRE binding factor2) is one of the bZIP type transcription factors, which are involved in abiotic stress response and ABA signaling in rice. Expression of OsABF2 is induced by ABA and various stress treatments. Findings show that survival rates of OsABF2 over-expressing Arabidopsis lines were increased under drought, salt, and heat stress conditions. The germination ratio of OsABF2 over-expressing Arabidopsis lines was decreased in the presence of ABA. Results indicate that OsABF2 over-expressing Arabidopsis lines have enhanced abiotic stress tolerance and have increased ABA sensitivity.

• Korean Journal of Plant Resources
• /
• 제30권5호
• /
• pp.571-577
• /
• 2017

Abscisic acid (ABA) is an important phytohormone involved in abiotic stress tolerance in plants. The group A bZIP transcription factors play important roles in the ABA signaling pathway in Arabidopsis but little is known about their functions in rice. In our current study, we have isolated and characterized a group A bZIP transcription factor in rice, OsABF3 (Oryza sativa ABA responsive element binding factor 3). We examined the expression patterns of OsABF3 in various tissues and time course analysis after abiotic stress treatments such as drought, salinity, cold, oxidative stress, and ABA in rice. Subcellular localization analysis in maize protoplasts using a GFP fusion vector further indicated that OsABF3 is a nuclear protein. Moreover, in a yeast one-hybrid experiment, OsABF3 was shown to bind to ABA responsive elements (ABREs) and its N-terminal region found to be necessary to transactivate a downstream reporter. A homozygous T-DNA insertional mutant of OsABF3 is more sensitive to salinity, drought, and oxidative stress compared with wild type plants ＆ OsABF3OX plants. In addition, this Osabf3 mutant showed a significantly decreased sensitivity to high levels of ABA at germination and post-germination. Collectively, our present results indicate that OsABF3 functions as a transcriptional regulator that modulates the expression of abiotic stress-responsive genes through an ABA-dependent pathway.