• 제목/요약/키워드: AUR1

검색결과 22건 처리시간 0.021초

Isolation of the Inositol Phosphoceramide Synthase Gene (AUR1) from Stress-Tolerant Yeast Pichia kudriavzevii

  • Yoo, Boung-Hyuk;Kim, Myoung-Dong
    • Journal of Microbiology and Biotechnology
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    • 제25권11호
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    • pp.1902-1907
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    • 2015
  • This study is the first report of the entire nucleotide sequence of an inositol phosphoceramide synthase gene from the stress-tolerant yeast Pichia kudriavzevii (PkAUR1). Sequence analysis revealed an open reading frame that spans 1,443 bp and encodes a 480-amino-acid-residue protein with the highest sequence similarity (41.7%) to Aur1 from Spathaspora passalidarum. A phenotypic assay with transformed S. cerevisiae and P. kudriavzevii indicated that two amino acid residues, Phe166 and Gly249, play crucial roles in the resistance to aureobasidin A, which is consistent with previous reports for other fungal Aur1s. The GenBank Accession No. for PkAUR1 is KP729614.

UNVEILING COMPLEX OUTFLOW STRUCTURE OF UY Aur

  • PYO, TAE-SOO;HAYASHI, MASAHIKO;BECK, TRACY;DAVIS, CHRISTOPHER J.;TAKAMI, MICHIHIRO
    • 천문학논총
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    • 제30권2호
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    • pp.109-112
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    • 2015
  • We present [$Fe\;{\small{II}}$] ${\lambda}1.257{\mu}m$ spectra toward the interacting binary UY Aur with 0".14 angular resolution, obtained with the Near infrared Integral Field Spectrograph (NIFS) combined with the adaptive optics system Altair of the GEMINI observatory. In the [$Fe\;{\small{II}}$] emission, UY Aur A (primary) is brighter than UY Aur B (secondary). The blueshifted and redshifted emission between the primary and secondary show a complicated structure. The radial velocities of the [$Fe\;{\small{II}}$] emission features are similar for UY Aur A and B: ${\sim}-100km\;s^{-1}$ and ${\sim}+130km\;s^{-1}$ for the blueshifted and redshifted components, respectively. Considering the morphologies of the [$Fe\;{\small{II}}$] emissions and bipolar outflow context, we concluded that UY Aur A drives fast and widely opening outflows with an opening angle of ${\sim}90^{\circ}$ while UY Aur B has micro collimated jets.

근접촉 식쌍성 ZZ Aur의 측광학적 연구 (A PHOTOMETRIC STUDY OF NEAR-CONTACT BINARY ZZ Aur)

  • 오규동;강영운;김호일;이우백
    • Journal of Astronomy and Space Sciences
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    • 제19권1호
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    • pp.31-38
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    • 2002
  • 2000년 2월부터 2001년 2월까지 소백산 천문대 61cm 망원경을 사용하여 CCD측광관측에 의한 ZZ Aur.(B, V & R)의 새로운 광도곡선을 얻었다. 이와 함께 새로운 광도곡선으로 부터 주극심시각 3점과 부극심시각 7점을 얻었다. 새로운 광도곡선을 WD model에 적용하여 이 별의 궤도 요소를 구하였다

Peculiar Features in the Emission Lines of Symbiotic Stars AG Draconis and UV Aurigae

  • Kim, Soo Hyun;Yoon, Tae Seog;Oh, Hyung-il
    • 천문학회보
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    • 제43권2호
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    • pp.59.3-59.3
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    • 2018
  • 공생별 AG Dra와 UV Aur에 대해 지난 10여년간 보현산천문대 1.8m망원경과 고분산 에셀 분광기 BOES(BOao Echelle Spectrograph)로 분광관측을 수행해 왔다. 최근 2017년 11월 - 2018년 6월 관측에서, AG Dra의 Fe II 방출선과 UV Aur $H{\alpha}$ 방출선이 예년과 다른 변화 모습을 보이고 있음을 찾아내었다. 이에 대한 변화 원인을 공전주기, 밝기 변화 또는 다른 이유와 연관지어 설명하고자 한다.

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NEAR-IR PHOTOMETRIC AND OPTICAL SPECTROSCOPIC STUDY OF THE FU ORIONIS OBJECT V582 AURIGAE

  • OH, HYUNG-IL;YOONY, TAE SEOG;SUNG, HYUN-IL
    • 천문학논총
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    • 제30권2호
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    • pp.269-270
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    • 2015
  • We carried out near-IR photometric and optical spectroscopic observations of V582 Aur, which is a FU Orionis type object, to investigate any periodic and/or aperiodic variations. We obtained light curves on the scale of a night and a year, in J, H and Ks bands with KASINICS (KASI Near Infrared Camera System) attached to the BOAO (Bohyun-san Optical Astronomy Observatory) 1.8-m reflector in Youngcheon, South Korea and examined photometric variations on the two time scales. So far we have not found any periodic brightness variations on the scale of a night. On the other hand, we have found that there seems to be a periodic brightness variation with a period of approximately 45 days. In addition, high-resolution optical spectroscopic observations of V582 Aur were performed from February 2013 to May 2014 with the high-resolution echelle spectrograph BOES attached to the BOAO 1.8-m reflector. We analyzed several spectral lines to understand the physical state of V582 Aur. The P Cyg profiles are clearly shown in the $H{\alpha}$ line and Na I D line.

Characterization of Xanthomonas axonopodis pv. glycines plasmids

  • Park, Sejung;Kim, Jung-Gun;Ingyu Hwang
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.135.2-136
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    • 2003
  • To characterize plasmids in Xanthomonu axonopodis pv. glycines, we isolated plasmids pAG1 from the strain AG1 and pXAG81 and PXAG82 from the strain Bra, respectively, and sequenced three plasmids. The size of plasmids, pAG1, pXAG81, and pXAG82 was 15,149-base pairs (bp), 26,727-bp, and 1,496-bp, respectively Fifteen and twenty six possible open reading frames (ORFs) were present in pAG1 and pXAG81, respectively. Only one ORF homologous to a rep gene of Xylella fastidiosa was present in pXAG82. pAG1 contained genes homologous to avrBs3, tnpA, tnpR, repA, htrA, three parA genes, M.XmaI, R.XmaI, and six hypothetical proteins. pXAG81 contained genes homologous to avrBs3, tnpA, tnpR, repA, htrA, two parA genes, pemI, pemK, mobA, mobB, mobC, mobD, mobE, trwB, traF, traH, ISxac2, and eleven hypothetical proteins. Based on DNA sequence analysis, we presume that pXAG81 is a conjugal plasmid. Interestingly, we found 0.5-kb truncated avirulence gene similar to aurXacE3 on the right border of avrBs3 homolgs of pAG1 and pXAG81. Two hundred twenty five isolates were analyzed to find aurBS3 or tra gene homologs by Southern hybridization. The numbers of avrBs3 homolog varied from 3 in AG1 to 8 in AG166. Two hundred seventeen isolates appeared to can conjugative plasmids (pXAG81 type), and thirty eight isolates appeared to carry non-conjugative plamids (pAGl type). This indicated that aurBs3 gene homologs might be spread by conjugation in X. axonopodis pv. glycines.

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Weakly Complementary Cycles in 3-Connected Multipartite Tournaments

  • Volkmann, Lutz;Winzen, Stefan
    • Kyungpook Mathematical Journal
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    • 제48권2호
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    • pp.287-302
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    • 2008
  • The vertex set of a digraph D is denoted by V (D). A c-partite tournament is an orientation of a complete c-partite graph. A digraph D is called cycle complementary if there exist two vertex disjoint cycles $C_1$ and $C_2$ such that V(D) = $V(C_1)\;{\cup}\;V(C_2)$, and a multipartite tournament D is called weakly cycle complementary if there exist two vertex disjoint cycles $C_1$ and $C_2$ such that $V(C_1)\;{\cup}\;V(C_2)$ contains vertices of all partite sets of D. The problem of complementary cycles in 2-connected tournaments was completely solved by Reid [4] in 1985 and Z. Song [5] in 1993. They proved that every 2-connected tournament T on at least 8 vertices has complementary cycles of length t and ${\mid}V(T)\mid$ - t for all $3\;{\leq}\;t\;{\leq}\;{\mid}V(T)\mid/2$. Recently, Volkmann [8] proved that each regular multipartite tournament D of order ${\mid}V(D)\mid\;\geq\;8$ is cycle complementary. In this article, we analyze multipartite tournaments that are weakly cycle complementary. Especially, we will characterize all 3-connected c-partite tournaments with $c\;\geq\;3$ that are weakly cycle complementary.

공주대학교 천문대의 경 ${\cdot}$ 위도 결정 (Determination of Longitude and Latitude of Kongju National University Observatory)

  • 김희수
    • 한국지구과학회지
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    • 제21권4호
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    • pp.389-397
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    • 2000
  • 본 연구에서는 경위의 TM-1A와 GPS(모델명: 4000SSI, GPS 45 Garmin corporation)를 활용하여 공주대학교 천문대의 위치를 결정하였다. 경위의를 이용한 관측에서는 2개의 별(${\alpha}$ Aur, ${\alpha}$ Boo)을 선정하여 남중시각과 남중고도를 측정한 다음위도와 경도를 얻어내었다. 또 GPS를 이용한 관측에서는 6개의 GPS 위성에서 보낸 자료를 수신하여 위도와 경도를 측정하였다. 공주대학교 천문대의 위치는 경도 127$^{\circ}$8'33'.16 , 위도 36$^{\circ}$ 28'14'.20로 결정되었다.

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Photoelectric Observation of the Long-Period Eclipsing Binaries at Yonsei University Observatory

  • Nha, Il-Seong;Lee, Yong-Sam;Chun, Yong-Woo;Kim, Ho-Il;Kim, Young-Soo
    • Journal of Astronomy and Space Sciences
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    • 제3권1호
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    • pp.1-13
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    • 1986
  • A long-term project(ten-year ; 1982-92) for the photoeletric observation in the UBV passbands of selected eclipsing binaries with P$\geq$10 days has initiated at Yonsei University Observatory using 40-cm and 61-cm reflectors. the instrumentatio used and the observation techniques and the reduction procedures applied to this investiagation are described. Out of 39 program stars, successful results have been obtained during our first two years for the 11 stars listed below, with the orbital period in days in the parenthesis, and the incomplete light curves of these stars are presented. SX Cas (36.57) AQ Cas (11.72) UU Cnc (96.71) RY Gem (9.30) V373 Cas (13.42) NY Cep (15.28) RX Cas (32.32) V396 Cas (15.28) ZZ Cnc (25.60) Zet Aur (982.2) Eps Aur (9885.) For the rest of the stars, the observations made in the first two years are not sufficient to attempt any meaning light curve construction ; some of the data are too fragmented and others show large scatter.

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산업용 Saccharomyces cerevisiae에서 Aspergillus awamori Glucoamylase 유전자의 발현 (Expression of Aspergillus awamori Glucoamylase Gene in an Industrial Strain of Saccharomyces cerevisiae)

  • 강동명;이수아;전영현;진종언;이황희;배석
    • 미생물학회지
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    • 제41권2호
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    • pp.146-151
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    • 2005
  • 전분 이용이 가능한 산업용 Saccharomyces cerevisiae균주를 개발하기 위해 alcohol dehydrogenase 유전자 프로모터(ADClp)의 조절하에 발현되는 Aspergillus awamori glucoamylase cDNA 유전자(GA1)를 산업용 S. cerevisiae의 염색체에 도입하였다. 산업적 이용에 적합한 효모균주를 얻기 위해 세균 ampicillin 저항성 유전자가 제거되고 GA1 유전자와 선별 표지유전자로 S. cerevisiae aureobasidin A 저항성 유전자(AUR1-C)와 재조합 부위로 Tyretrotransposon $\delta$-서열이 포함된 integrative cassette를제조하였다. 이 $\delta-integrative$ cassette로 형질전환된 산업용 S. cerevisiae는 배지상에 glucoamylase를 생산 분비하였고 전환을 유일한 탄소원으로 하여 생장하였다. 형질전환체를 비선택배지에서 배양했을 매 삽입된 GA1유전자가 100세대까지 안정되게 유지되었다.