• Journal of Microbiology and Biotechnology
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• v.17 no.2
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• pp.342-347
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• 2007

Bacteriophage ${\Phi}FC1$ integrase (MJ1) was previously shown to perform a site-specific recombination between a phage attachment site (attP) and a host attachment site (attB) in its host, Enterococcus faecalis, and also in a non-host bacterium, Escherichia coli. Here, we investigated biochemical features of MJ1 integrase. First, MJ1 integrase could perform in vitro recombination between attP and attB in the absence of additional factors. Second, MJ1 integrase interacted with att sites. Electrophoretic mobility shift assays and DNase I footprinting revealed that MJ1 integrase could efficiently bind to all the att sites and that MJ1 integrase recognized relatively short sequences (${\sim}50bp$) containing an overlapping region within attB and attP. These results demonstrate that MJ1 integrase indeed catalyzes an integrative recombination between attP and attB, the mechanism of which might be simple and unidirectional, as found in serine integrases.

• Microbiology and Biotechnology Letters
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• v.39 no.4
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• pp.337-344
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• 2011

Integrase MJ1 from the bacteriophage ${\Phi}FC1$ carries out recombination between two DNA sequences (the phage attachment site, attP and the bacterial attachment site, attB) in NIH3T3 mouse cells. In this study, the integration vector containing attP, attB and the integrase gene MJ, was constructed. The integration mediated by integrase MJ1 in Escherichia coli led to excision of LacZ. Therefore, the frequency of integration was measured by the counting of the white colony, which is detectable on X-Gal plates. The extrachromosomal integration in NIH3T3 mouse cells was monitored by the expression of the green fluorescent protein (GFP) as a reporter. To demonstrate integration mediated integrase MJ1 in NIH3T3 cells, vectors containing attP and attB were co-transfected into NIH3T3 cells. The integration was confirmed by fluorescent microscopy. The expression of GFP was induced in NIH3T3 cells expressing MJ1 without accessory factors. By contrast, the excision mediated by the MJ1 between attR and attL had no effect on the expression of GFP. These results suggest that integrase MJ1 may enable a variety of genomic modifications for research and therapeutic purposes in higher living cells.

• Korean Journal of Microbiology
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• v.41 no.2
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• pp.140-145
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• 2005

Streptomyces natalensis ATCC27448 produces natamycin, a commercially important macrolide antifungal antibiotic. For molecular genetic study of S. natalensis, we have developed a system for introducing DNA into S. natalensis via conjugal transfer from Escherichia coli. An effective transformation procedure for S. natalensis was established based on transconjugation from E, coli ET12567/pUZ8002 using a ${\Phi}C31$-derived integration vector, pSET152, containing oriT and attP fragments. The high frequency was obtained on MS medium containing 10 mM $MgCl_2$ using $6.25\times10^8$ of E.coli donor cells without heat treatment of spores. In addition, southern blot analysis of exconjugants and the sequence of plasmids containing DNA flanking the insertion sites from the chromosome revealed that S. natalensis contains a single ${\Phi}C31$ attB site and at least a secondary or pseudo attB site. Similar to the case of various Streptomyces species, a single ${\Phi}C31$ attB site of S. natalensis is present within an ORF encoding a pirin-homolog, but a pseudo-attB site is present within a distinct site (GenBank accession no. $YP\_117731$) and also its sequence deviates from the consensus sequences of attB sequence.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1996.06c
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• pp.219-227
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• 1996

Since the introduction of animal traction technologies(ATT) in many Sub Saharan Africa (SSA) countries, attempts to propagate its widespread use in the continent have suffered several setbacks. Many factors are responsible of this. However, developments in the African economies are believed to be a prominent factor. This study provides empirical evidence of the impact of economic growth on the performance of ATT-in Sub-Saharan Africa(SSa). The analysis uses 1971-1990 time-series data on ATT from botswana. The performance of ATT is measured on the basis of changes year covered under this study. The models used, are a regression model and a trend projection model. Although the regression model is representing a simplified view of the relationship between ATT and economic growth , it takes account of the influence of annual amounts of rainfall. It is concluded that economic growth has had a negative impact on the performance of ATT in Botwana. As the country's Gross Domestic Pro uct (GDP) steadily increased over the period of the analysis, the number of households using ATT declined at a rate of 2.5% per year. The impact of the GDP on ATT was directly associated with increases in the use of tractor, food imports and beef exports. The results have serious policy implications for agricultural development in many African countries that are not capable of sustaining their economic growth.

• Journal of Microbiology and Biotechnology
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• v.5 no.3
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• pp.132-137
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• 1995

The lambda integrase (lnt) is believed to bind to several arm and core sites of attP DNA in order to facilitate intasome formation. We have done systematic mutagenic analysis on all 5 arm sites and found that P1 is absolutely required for integration while P2 is not. We also found that all 3 P' arm sites(P'1, P'2, and P'3) are required for efficient integrative recombination. P'1, which is an important binding site for excision, also seems to be crucial for integration when preincubation of attP DNA with Int and IHF is performed before recombination. Preincubation assay revealed that preincubation with Int and IHF improved the efficiency of recombination of wild type attP DNA and demolished recombinations of P'1 mutant attP DNAs.

• Proceedings of the Korean Information Science Society Conference
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• 2002.10d
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• pp.676-678
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• 2002

질의 응답 시스템(Question Answering: QA)에서 정답 유형 부류(Answer Type Taxonomy: ATT)란 사용자 질문 분석을 위한 미 부류 체계를 의미하는 것으로, ATT의 크기가 클수록 시스템의 성능은 높아진다. ATT를 확장하기 위해서는, 개체(Named Entity)에 의미 범주를 결정하는 개체 분류기(Named Entity Tagger의 분류 체계가 세분되어야 하는데, 기존의 개체 분류기는 한문서 내에서 그 개체의 분류를 시도하기 때문에, 분류를 위한 문맥 정보의 양이 부족하여, 정확하고 상세한 분류를 기대하기 힘들다. 본 논문에서는 동일 개체에 대한 문맥 정보를 수집하기 위해, 그 개체가 나타나는 다른 문서들을 검색하는 개체 피드백 Named Enti쇼 Feedback)이라는 기법을 사용한다. 개체가 상세히 분류됨에 따라 ATT도 확장될 수 있었으며, 이렇게 확장된 ATT상에서의 정답 추출은 baseline보다 약 7%정도의 성능 향상을 보여, 개체 피드백의 효과를 확인할 수 있었다.

• Journal of Information Technology Services
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• v.17 no.3
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• pp.77-100
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• 2018

As the importance of R&D has recently become more important, the budget for national R&D projects has continued to grow. Along with, there is a growing awareness to use and manage R&D funds transparently. This study examines the relationship among attitude toward the behavior (ATT), subjective norm (SN), perceived behavioral control (PBC) and behavioral Intention (BI) deriving from TPB (Theory of planned Behavior). To empirically prove the hypothesis, the statistical analysis were conducted based on the response from the 2,021 RCMS users, using SPSS 21.0 and AMOS 21.0. The research model variable consists of ATT, SN, PBC, BI, person type, RCMS characteristics. The results of study can be summarized as follows. First, ATT, SN, PBC have the positive effect on BI. Second, According to the type of person (Director, R&D supporter), there is no difference in ATT on BI but there is a difference in SN (or PBC) on BI. Third, The type of person have moderating effects in the relationship between ATT and BI. Finally, All RCMS characteristics have moderating effects in the relationship between ATT and BI. Some RCMS characteristics have moderating effects in the relationship between PBC and BI.

• Journal of Aquaculture
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• v.10 no.2
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• pp.143-151
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• 1997

A six week feeding trial was conducted to determine the amount of fish meal analog (FMA) that can be replacing fish meal protein (FM) in Korean Rockfish. Seven experimental diets were formulated on isonitrogenous 52% crude protein and isocaloric basic 16.8KJ/g diet. Also, foreign commercial fish meal analog (CFMA) and attractants (ATT) were tested in this experiment. Percentage of the graded level of replacement of FM by FMA/CFMA on the basis of crude protein were as following : Diet 1, 100%FM ; Diet by 2, 60%FM : 40% CFMA ; Diet 3, 60%FM : 40$ CFMA+ATT ; Diet 4, 80%FM : 20%FMA ; Diet 5, 80%FM : 20% FMA+ATT ; Diet 6, 60%FM : 40%FMA+ATT ; Diet 7, 40%FM : 60% FMA+ATT. The FMA was made by mixing six animal protein source such as th blood meal, squid liver powder, meat and bond meal, leather meal, feather meal, poultry by-product and 3 essential amino acids (Met, Lys, Ile). Weight gain, feed efficiency, specific growth rate and protein efficiency ratio of fish fed diets 4, 5 and 6 were not significantly different (P>0.05) from those of fish fed the control (100% FM), while those of fish fed diets 2 and 3 were significantly lower (P<0.05) than those of fish fed the diet 6. There was no significant ATT effects in this study (P>0.05). Significant differences were found in hepatosomatic index, hemoglobin and condition factor. Therefore, these, results indicated that FMA can be used up to 40% as a substitute of fish meal protein in Korean Rockfish diets.

• Journal of the Korean Society of Radiology
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• v.82 no.3
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• pp.626-637
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• 2021

Purpose To explore cerebrovascular reservoir (CVR) and arterial transit time (ATT) changes using acetazolamide-challenged multi-phase arterial spin labeling (MP-ASL) perfusion-weighted MRI in chronic cerebrovascular steno-occlusive disease. Materials and Methods This retrospective study enrolled patients with chronic steno-occlusion who underwent acetazolamide-challenged MP-ASL between June 2019 and October 2020. Cerebral blood flow, CVR, basal ATT, and ATT changes associated with severe stenosis, total occlusion, and chronic infarction lesions were compared. Results There were 32 patients (5 with bilateral steno-occlusion) in our study sample. The CVR was significantly reduced during total occlusion compared with severe stenosis (26.2% ± 28.8% vs. 41.4% ± 34.1%, respectively, p = 0.004). The ATT changes were not significantly different (p = 0.717). The CVR was marginally lower in patients with chronic infarction (29.6% ± 39.1% vs. 38.9% ± 28.7%, respectively, p = 0.076). However, the ATT was less shortened in patients with chronic infarction (-54 ± 135 vs. -117 ± 128 ms, respectively, p = 0.013). Conclusion Acetazolamide-challenged MP-ASL provides an MRI-based CVR evaluation tool for chronic steno-occlusive disease.

• Journal of Life Science
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• v.20 no.1
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• pp.97-102
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• 2010

Streptomyces platensis YK-2, newly isolated from forest soil, produces transglutaminase (TGase), which catalyses an acyl transfer reaction between the primary grade amine and protein or $\gamma$-carboxyamide group of peptide bound glutamine residues. For a molecular genetic study of S. platensis, an effective transformation method was established by using a conjugal transfer of DNA from Escherichia coli to spores of actinomycetes. The highest transconjugation frequency of S. platensis was obtained on an MS medium containing 50 mM $MgCl_2$, using $5{\times}10^7\;E$. coli as a DNA donor and $1{\times}10^8$ spores without heat treatment as a host. We also identified that S. platensis contains a single attB site within an ORF encoding a pirin-homolog, and that its attB site sequence shows high homology to that of S. logisporoflavus. In addition, it was confirmed by phenotypic analyses of exconjugants that the introduction of heterologous DNA into the attB site of the S. platensis chromosome does not affect its morphological differentiation and TGase production.