• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1687-1689
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• 2008

In the detection of pathogenic microorganisms ATP-bioluminescence reaction is a fascinating method. ATP(adenosine triphosphate) is an energy source of all kinds of living organism and ATP-bioluminescence reaction uses this ATP. However, ATP exists not only in the cells but also outside the cells. Therefore ATP-bioluminescence reaction only with intracellular ATP is very important in pathogenic microorganism detection. Because of that reason we developed a microfluidic channel containing Dielectrophoretic zone which capture microorganisms and eliminating and washing extracellular ATP with ATP-degarading enzymes, adenosine phosphate deaminase and apyrase. Microorganisms are captured by pDEP force at the DEP electrode zone and only extracellular ATPs are washed and eliminated outside the zone.

• BioChip Journal
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• v.12 no.4
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• pp.287-293
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• 2018

Bacillus cereus can cause blood infections (i.e., sepsis). Its early detection is very important for treating patients. However, an antibody with high binding affinity to B. cereus is not currently available. Bacteriophage cell wall-binding domain (CBD) has strong and specific binding affinity to B. cereus. Here, we report the improvement in the sensitivity of an ATP bioluminescence assay for B. cereus detection using CBD-conjugated magnetic nanoparticles (CBD-MNPs). The assay was able to detect as few as 10 colony forming units (CFU) per mL and $10^3CFU\;per\;mL$ in buffer and blood. CBD-MNPs did not show any cross-reactivity with other microorganisms. These results demonstrate the feasibility of the ATP assay for the detection of B. cereus.

• Journal of Microbiology and Biotechnology
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• v.33 no.11
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• pp.1506-1512
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• 2023

Quantitative analysis of adenosine triphosphate (ATP) has been widely used as a diagnostic tool in the food and medical industries. Particularly, the pathogenesis of a few diseases including inflammatory bowel disease (IBD) is closely related to high ATP concentrations. A bioluminescent D-luciferin/luciferase system, which includes a luciferase (FLuc) from the firefly Photinus pyralis as a key component, is the most commonly used method for the detection and quantification of ATP. Here, instead of isolating FLuc produced in recombinant Escherichia coli, we aimed to develop a whole-cell biocatalyst system that does not require extraction and purification of FLuc. To this end, the gene coding for FLuc was introduced into the genome of probiotic Saccharomyces boulardii using the CRISPR/Cas9-based genome editing system. The linear relationship (r² = 0.9561) between ATP levels and bioluminescence generated from the engineered S. boulardii expressing FLuc was observed in vitro. To explore the feasibility of using the engineered S. boulardii expressing FLuc as a whole-cell biosensor to detect inflammation biomarker (i.e., ATP) in the gut, a colitis mouse model was established using dextran sodium sulfate as a colitogenic compound. Our findings demonstrated that the whole-cell biosensor can detect elevated ATP levels during gut inflammation in mice. Therefore, the simple and powerful method developed herein could be applied for non-invasive IBD diagnosis.

• Animal Bioscience
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• v.36 no.10
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• pp.1604-1611
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• 2023

Objective: The aim of this study was to investigate the protective effect of wheat phytase as a structural decomposer of inflammatory nucleotides, extracellular adenosine triphosphate (ATP), and uridine diphosphate (UDP) on HT-29 cells. Methods: Phosphatase activities of wheat phytase against ATP and UDP was investigated in the presence or absence of inhibitors such as L-phenylalanine and L-homoarginine using a Pi Color Lock gold phosphate detection kit. Viability of HT-29 cells exposed to intact- or dephosphorylated-nucleotides was analyzed with an EZ-CYTOX kit. Secretion levels of pro-inflammatory cytokines (IL-6 and IL-8) in HT-29 cells exposed to substrate treated with or without wheat phytase were measured with enzyme-linked immunosorbent assay kits. Activation of caspase-3 in HT-29 cells treated with intact ATP or dephosphorylated-ATP was investigated using a colorimetric assay kit. Results: Wheat phytase dephosphorylated both nucleotides, ATP and UDP, in a dose-dependent manner. Regardless of the presence or absence of enzyme inhibitors (L-phenylalanine and L-homoarginine), wheat phytase dephosphorylated UDP. Only L-phenylalanine inhibited the dephosphorylation of ATP by wheat phytase. However, the level of inhibition was less than 10%. Wheat phytase significantly enhanced the viability of HT-29 cells against ATP- and UDP-induced cytotoxicity. Interleukin (IL)-8 released from HT-29 cells with nucleotides dephosphorylated by wheat phytase was higher than that released from HT-29 cells with intact nucleotides. Moreover, the release of IL-6 was strongly induced from HT-29 cells with UDP dephosphorylated by wheat phytase. HT-29 cells with ATP degraded by wheat phytase showed significantly (13%) lower activity of caspase-3 than HT-29 cells with intact ATP. Conclusion: Wheat phytase can be a candidate for veterinary medicine to prevent cell death in animals. In this context, wheat phytase beyond its nutritional aspects might be a novel and promising tool for promoting growth and function of intestinal epithelial cells under luminal ATP and UDP surge in the gut.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.57 no.2
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• pp.102-108
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• 2008

AC generator plays an important role of power system. The large AC generator fault may lead to large impacts or perturbations in power system. And then the protection of a generator has very important role in maintaining stability in a power system. In present, the DFT(discrete Fourier transform) based RDR(ratio differential relay) had been widely applied to a internal fault of a generator stator winding. But DFT has a serious drawback. In the course of transforming a target signal to frequency domain, time information is lost. DWT uses a time-scale region. This paper proposes an advanced fault detection algorithm using DWT(discrete Wavelet transform) to enhance the drawback of conventional DFT based relaying. To evaluate the performance of the proposed relaying, we used the test data which were sampled with 720 [Hz] per cycle and obtained from ATP(alternative transient program) simulation. And we made a comparative study of conventional DFT based RDR and the proposed relaying.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.57 no.4
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• pp.377-382
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• 2008

For stator winding protection of AC generator, KCL(Kirchhoff's Current Law) is widely applied. Actually a CRDR(Current Ratio Differential Relay) based on DFT(Discrete Fourier Transform) has been used for protecting generator. It has been pointed out that defects can occur during the process of transforming a time domain signal into a frequency domain one which can lead to loss of time domain information. Wavelets techniques are proposed for the analysis of power system transients. This paper introduces an algorithm to choose a suitable Mother Wave1et for generator stator fault detection. For optimal selection, we analyzed db(Daubechies), sym(Symlets), and coif(Coiflects) of Mother Wavelet. And we compared with performance of the choice algorithm using detail coefficients energy and RMS(root mean square) error. It can be improved the reliability of the conventional DFT based CRDR. The feasibility and effectiveness of the proposed scheme is proved with simulation using collected data obtained from ATP (Alternative Transient Program) package.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.4
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• pp.288-292
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• 2006

Through statistically designed experiments, lysis agents were optimized to effectively disrupt bacterial cells in a microfluidic device. Most surfactants caused the efficient lysis of Gram-positive microbes, but not of Gram-negative bacteria. A Plackett-Burman design was used to select the components that increase the efficiency of the lysis of the Gram-negative bacteria Escherichia coli. Using this experimental design, both lysozyme and benzalkonium chloride were shown to significantly increase the cell lysis efficiency, and ATP was extracted in proportion to the lysis efficiency. Benzalkonium chloride affected the cell membrane physically, while lysozyme destroyed the cell wall, and the amount of ATP extracted increased through the synergistic interaction of these two components. The two-factor response-surface design method was used to determine the optimum concentrations of lysozyme and benzalkonium chloride, which were found to be 202 and 99 ppm, respectively. The lysis effect was further verified by microscopic observations in the microchannels. These results indicate that Gram-negative cells can be lysed efficiently in a microfluidic device, thereby allowing the rapid detection of bacterial cells using a bioluminescence-based assay of the released ATP.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.58 no.2
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• pp.136-141
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• 2009

An AC generator is an important components in producing a electric power and so it requires highly reliable protection relays to minimize the possibility of demage occurring under fault conditions. Conventionally, a DFT based RDR has been used for protecting the generator stator winding. However, when DFTs based on Fourier analysis are used, it has been pointed out that defects can occur during the process of transforming a time domain signal into a frequency domain one which can lead to loss of time domain information. This paper proposes the internal fault detection and fault type discrimination for the stator winding by applying the detailed coefficients by Daubechies Wavelet Transform to overcome the defects in the DFT process. For the case studies reported in the paper, a model system was established for the simulations utilizing the ATP, and this verified the effectiveness of the proposed technique through various off-line tests carried out on the collected data. The propose method is shown to be able to rapidly identify internal fault and did not operate a miss-operation for all the external fault tested.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.9
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• pp.4171-4176
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• 2012

The conventional block systems for railway signaling currently in operation in Korea have not been electrified or integrated and therefore there have been difficulties in terms of construction and maintenance. Two independent systems have been installed for ABS and LEU of ATP system (for speeded-up lines), although they deal with the same signaling information at the same location in order to control the trains. In these conventional ABS and LEU, a number of duplicate modules are installed in each device including lamp detection units and power supply units and it results increased manufacturing costs and maintenance efforts. This paper deals with the prototype development of integrated electronic block system with CPU-based digital control methods in order to overcome the limitations of the conventional ABS. The suggested system is the integration of the conventional ABS with LEU of ATP and it is also applicable for the non-ATP sections as well.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10433-10438
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• 2015

Background: Acute lymphoblastic leukemia (ALL) is the most common cancer diagnosed in children and represents approximately 25% of cancer diagnoses among those younger than 15 years of age. Aim and Objectives: This study investigated substitutions in the ATP synthase subunit 6 gene of mitochondrial DNA (mtDNA) as a potential diagnostic biomarker for early detection and diagnosis of acute lymphoblastic leukemia. Based on mtDNA from 23 subjects diagnosed with acute lymphoblastic leukemia, approximately 465 bp of the ATP synthase subunit 6 gene were amplified and sequenced. Results: The sequencing revealed thirty-one mutations at 14 locations in ATP synthase subunit 6 of mtDNA in the ALL subjects. All were identified as single nucleotide polymorphisms (SNPs) with a homoplasmic pattern. The mutations were distributed between males and females. Novel haplotypes were identified in this investigation: haplotype (G) was recorded in 34% in diagnosed subjects; the second haplotype was (C) with frequency of 13% in ALL subjects. Neither of these were observed in control samples. Conclusions: These haplotypes were identified for the first time in acute lymphoblastic leukemia patients. Five mutations able to change amino acid synthesis for the ATP synthase subunit 6 were associated with acute lymphoblastic leukemia. This investigation could be used to provide an overview of incidence frequency of acute lyphoblastic leukemia (ALL) in Saudi patients based on molecular events.