• Journal of Embryo Transfer
• /
• v.29 no.3
• /
• pp.221-228
• /
• 2014

Despite that porcine spermatogonial stem cells (pSSCs) have been regarded as a practical tool for preserving eternally genetic backgrounds derived from pigs with high performance in the economic traits or phenotypes of specific human diseases, there were no reports about precise definition of niche conditions promoting proliferation and maintenance of pSSCs. Accordingly, we tried to determine niche conditions supporting proliferation and maintenance of undifferentiated pSSCs for short-term. For these, undifferentiated pSSCs were progressively cultured in different composition of culture medium, seeding density of pSSCs, type of feeder cells and concentration of growth factors, and then total number of and alkaline phosphatase (AP) activity of pSSCs were investigated at post-6 day culture. As the results, the culture of $4{\times}10^5$ pSSCs on mitotically in activated $2{\times}10^5$ STO cells in the mouse embryonic stem cell culture medium (mESCCM) supplemented with 30 ng/ml glial cell line-derived neurotrophic factor (GDNF) was identified as the best niche condition supporting effectively the short-term maintenance of undifferentiated pSSCs. Moreover, the optimized short-term culture system will be a basis for developing long-term culture system of pSSCs in the following researches.

• Journal of Biosystems Engineering
• /
• v.20 no.1
• /
• pp.95-103
• /
• 1995

A series of study has been conducted to develop a multi-purpose fruit grader using a black ＆ white image processing system equipped with a 550 nm interference filter. A device and high performance algorithms were developed for sizing and color grading of Fuji apple in the previous study. In this study an emphasis was put on finding correlations between weights of several kinds of fruits and their area fractions(AF), and on compensating the blurring effect upon sizing and color grading by conveying speed of fruit. Also, the effect of orientation and direction of fruit on conveyor during image forming was analyzed to identify any difficulty (or utilizing an automatic fruit feeder. The results are summarized as follows. 1. The correlation coefficients(r) between the weights of fruits and their image sizes were 0.984~0.996 for apples, 0.983~0.990 for peachs, 0.995 for tomato, 0.986 for sweet persimmon and 0.970~0.993 for pears. 2. It was possible to grade fruits by color with the area weighted mean gray values(AWMGV) based on the mean gray valves of direct image and the compensated values of reflected image of a fruit, and also possible to sort fruits by size with AF. Accuracies in sizing and color grading ranged over 81.0% ~95.0% and 82.0% ~89.7% respectively as compared with results from sizing by electronic weight scale and grading by expert. 3. The blurring effect on the sizing and color grading depending on conveying speed was identified and regression equations were derived. 4. It was found that errors in sizing and coloring grading due to the change in direction and orientation of Fuji apple on the conveyor were not significant as far as the stem end of apple keeping upward.

• Reproductive and Developmental Biology
• /
• v.37 no.3
• /
• pp.143-148
• /
• 2013

Spermatogenesis is initiated from spermatogonial stem cells (SSCs) that has an ability of self-renewal and unipotency to generate differentiating germ cells. The objective of this study is to develop the simple method for derivation of SSCs using non-sorting of both spermatogonia and feeder cells. Simply uncapsulated mouse testes were treated with enzymes followed by surgical mincing, and single cells were cultured in stempro-$34^{TM}$ cell culture media at $37^{\circ}C$. After 5 days of culture, aciniform of SSC colony was observed, and showed a strong alkaline phosphatase activity. Molecular characterization of mouse SSCs showed that most of the mouse SSC markers such as integrin ${\alpha}6$ and ${\beta}1$, CD9 and Stra8. In addition, pluripotency embryonic stem cell (ESC) marker Oct4 were expressed, however Sox2 expression was lowered. Interestingly, expression of SSC markers such as Vasa, Dazl and PLZF were stronger than mouse ESC (mESC). This data suggest that generated mouse SSCs (mSSCs) in this study has at least similar biomarkers expression to mESC and mSSCs derived from other study. Immunocytochemistry using whole mSSC colony also confirmed that mSSCs generated from this study expressed SSC specific biomarkers such as c-kit, Thy1, Vasa and Dazl. In conclusion, mSSCs from 5 days old mouse testes were successfully established without sorting of spermatogonia, and this cells expressed both mESC and SSC specific biomarkers. This simple derivation method for mSSCs may facilitate the study of spermatogenesis.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.40 no.12
• /
• pp.2461-2468
• /
• 2015

Sinuous antenna is utilized in a number of application like direction-finding system that require ultra wideband, but because of high input impedance of the antenna, it is difficult to design low-profile antenna and balun. In this study, we used Babinet's principle and proposed linear 2-arm slot sinuous antenna that is divided sinuous 6 cell with equal spacing between the most largest radius $R_1=31.83mm$ and the smallest radius $R_p=1.7mm$ instead of exponential 2-arm strip sinuous antenna for 2-18[GHz] frequency band to obtain low input impedance, and we have studied -10dB bandwidth of antenna when has been fed by $80{\Omega}$ instead of $188{\Omega}$ at feeder point. As a result, we have obtained return loss below than -10dB for about 4-18[GHz] frequency range in the case of slot antenna, but at all frequency band, return loss was above -10dB in the case of strip antenna.

• Journal of Korean Society of Transportation
• /
• v.26 no.5
• /
• pp.227-235
• /
• 2008

This study analyzed the efficiency of a trunk with branches and a trunk with feeders route system comparing the user cost and operator cost of those route systems. Basically, a trunk with branches is more efficient than a trunk with feeders in the passengers' perspective if the transit system of a trunk and feeders are the same. In the case that the trunk system is hierarchically higher than the feeder system, a trunk with feeders has the competitive edge over a trunk with branches if the saving of travel cost induced by using the trunk line is larger than the increasing waiting and transfer cost. This result is consistent with the previous research by Park et al.(2007c), which analyzed the efficiency of hierarchical structure in a grid transit network. If the travel demand of each origins and destinations is low, increasing headway according to the travel demand can increase passenger load or operating efficiency in a trunk with feeders route system. In this case, a trunk with feeders route system is more prevalent as the operating cost, route length, and number of destinations increases, while it is less prevalent as the transfer cost and passengers' time value of the waiting time increases. In cases that central business district, which is located in the middle of a trunk line, generates travel demand, a trunk with feeders is more efficient than a trunk with branches with the increasing travel demand from and to the CBD. Therefore it can be concluded that to have a dual operating system between CBD and suburban is more efficient than one type transit system. The efficiency of that system can be increased through an efficient transfer system that can reduce the transfer cost.

• Journal of the Korean Society for Railway
• /
• v.19 no.3
• /
• pp.314-323
• /
• 2016

Overhead line voltage of DC railway traction substations has rising or falling characteristics depending on the acceleration and regenerative braking of the subway train loads. The suppression of this irregular fluctuation of the line voltage gives rise to improved energy efficiency of both the railway substation and the trains. This paper presents parameter estimation schemes using the RC circuit model for an overhead line voltage at a 1500V DC electric railway traction substation. A linear artificial neural network with a back-propagation learning algorithm was trained using the measurement data for an overhead line voltage and four feeder currents. The least square estimation method was configured to implement batch processing of these measurement data. These estimation results have been presented and performance analysis has been achieved through raw data simulation.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.9 no.6
• /
• pp.1599-1606
• /
• 2008

Recently, new dispersed generation systems such as photovoltaic, wind power, fuel cell etc. are energetically interconnected and operated in the distribution systems, as one of the national projects for alternative energy with the provision against oil crisis. The technical guidelines on the interconnection of dispersed generation systems have been established and conducted positively. However, protection devices (Re-closer) in primary feeder of distribution system interconnected with photovoltaic generation may cause problems with mis-operation, and then many customers could have problems like an interruption. So, this paper presents the optimal method to minimize the impact of interruption, using both the symmetric method and MATLAB/SIMULINK. And, also this paper shows the effectiveness of proposed method by simulating at the real distribution systems.

• Korean Chemical Engineering Research
• /
• v.44 no.6
• /
• pp.631-635
• /
• 2006

To investigate the characteristics of substitute natural gas (SNG) production from direct coal methanation, the continuous lab-scale entrained flow hydrogasifier (I.D. : 0.025 m, Height : 1.0 m) was used in this experiment. The hydrogasification system consisted of high pressure gas handling system, high pressure coal feeder, entrained flow hydrogasifier, and unreacted char separator. The experiment was performed at the various conditions of reaction temperature ($600{\sim}800^{\circ}C$), $H_2$/coal ratio (0.2~0.4), and coal feed rate (0.8~2.5 g/min). Although it was shown that carbon conversion was different trends with coals from the methanation results for 6 sample coals, the carbon conversion increased with increasing reaction temperature. And it increased with increasing H2/coal ratio, whereas the concentration of CH4 decreased. Also. the carbon conversion increased with the carbon content of coal sample and had a maximum value at volatile matter content of 35 wt％.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.31 no.3B
• /
• pp.205-209
• /
• 2011

The low carrying capacity caused by the deposition in a sewer line is one of the main reason of the urban flood. Therefore, an efficient maintenance and management of the storm water drainage system is very important to prevent urban flood. In this research, the sediment transport characteristics through a pressure pipeline were examined with laboratory experiments. Bed-forms in a pipeline, sediment rates, roughness due to sediments were examined. Experimental system consists of flow circulation system with a pump and a sediment feeder at the upstream of the pipeline. Sediments were supplied into a 60 mm-diameter and 8 m-long pipe. Maximum flow rate is $30m^3/hr$, and the sediment feeding rate range is 5 g/s~19 g/s. Governing parameters and estimation equation for sediment transport rate were developed. The mean velocity (U), coefficient of viscosity (${\mu}$), unit width bed load ($q_b$), mean diameter of particle ($d_{50}$), unit weight of sediment in water (${\gamma}^{\prime}_s$) were adopted as the most influencing factors of sediment transport patterns. The prediction equation for sediment transport rate were developed with two dimensionless terms. These two dimensionless terms showed a linear relationship with high correlation coefficient.

• Korean Journal of Food Science and Technology
• /
• v.49 no.2
• /
• pp.162-167
• /
• 2017

A Katsuobushi smoking machine was developed and evaluated to determine its benzo(a)pyrene reducing effect. The machine was equipped with two heaters for smoking and chamber heating. The smoke-generating system was equipped with a cadmium sulfide (CdS) smoke sensor, an on/off controller, and a rotating feeder with a smoke inlet. Raw bonito was steamed and then smoked under three smoke levels. After smoking at $45^{\circ}C$ for 108 h, the benzo(a)pyrene concentrations were 5.87, 7.83, and $11.41{\mu}g/kg$ at the low, middle, and high smoke levels, respectively. The benzo(a)pyrene concentrations after low-level smoking at 45, 65, and $85^{\circ}C$ for 108 h were 5.87, 4.82, and $3.27{\mu}g/kg$, respectively. Accordingly, the optimal conditions for benzo(a)pyrene reduction were a lower smoke level and higher smoking temperature. These optimal smoking conditions can be implemented with the newly developed machine, but is not possible using a conventional Katsuobushi smoking machine.