• Biomedical Science Letters
• /
• v.25 no.2
• /
• pp.190-195
• /
• 2019

Hemoglobin (Hb) concentrations and hematocrit (Hct) values can be changed by factors such as erythrocyte production, destruction, and bleeding. In addition, variants in the protein expression involved in the amount of red blood cells that determine Hb metabolism or Hct value can increase susceptibility to complex blood diseases. Previous studies have reported significant single nucleotide polymorphisms (SNPs) by applying a genome-wide association study (GWAS) on Hb levels and Hct values in European population. In this study, we confirmed whether the significant SNPs are replicated in Koreans. In previous studies, 26 and 18 SNPs with a significant correlation Hb and Hct were identified in Korean genotype data, and 21 and 12 SNPs were selected, respectively. The SNPs of PRKCE (rs10495928), TMPRSS6 (rs2235321, rs5756505, rs855791) were significantly associated with Hb (P<0.05). In the association analysis of Hct, the SNPs of HBS1L (rs6920211, rs9389268, rs9483788), PRKCE (rs4953318), SCGN (rs9348689) and TMPRSS6 (rs2413450) genes showed a significant correlation (P<0.05). Replicated SNPs and not replicated SNPs showed the difference of genetic distance calculated by Fst. The replicated SNPs with a significant correlation showed similar allele frequencies, whereas the not replicated SNPs showed a large difference in allele frequency. All replicated SNPs with significant correlations had Fst values less than 0.05, indicating that the genetic distance between the groups was close. On the other hand, the not replicated SNPs showed that the Fst value was 0.05 or more and the genetic distance was relatively large.

• Journal of Animal Science and Technology
• /
• v.60 no.12
• /
• pp.31.1-31.10
• /
• 2018

Background: Research on loci influencing milk production traits of dairy cattle is one of the main topics of investigation in livestock. Many genomic regions and polymorphisms associated with dairy production have been reported worldwide. In this context, the purpose of this study was to identify candidate loci associated with milk yield in Argentinean dairy cattle. A database of candidate genes and single nucleotide polymorphisms (SNPs) for milk production and composition was developed. Thirty-nine SNPs belonging to 22 candidate genes were genotyped on 1643 animals (Holstein and Holstein x Jersey). The genotypes obtained were subjected to association studies considering the whole population and discriminating the population by Holstein breed percentage. Phenotypic data consisted of milk production values recorded during the first lactation of 1156 Holstein and 462 Holstein ${\times}$ Jersey cows from 18 dairy farms located in the central dairy area of Argentina. From these records, 305-day cumulative milk production values were predicted. Results: Eight SNPs (rs43375517, rs29004488, rs132812135, rs137651874, rs109191047, rs135164815, rs43706485, and rs41255693), located on six Bos taurus autosomes (BTA4, BTA6, BTA19, BTA20, BTA22, and BTA26), showed suggestive associations with 305-day cumulative milk production (under Benjamini-Hochberg procedure with a false discovery rate of 0.1). Two of those SNPs (rs43375517 and rs135164815) were significantly associated with milk production (Bonferroni adjusted p-values < 0.05) when considering the Holstein population. Conclusions: The results obtained are consistent with previously reported associations in other Holstein populations. Furthermore, the SNPs found to influence bovine milk production in this study may be used as possible candidate SNPs for marker-assisted selection programs in Argentinean dairy cattle.

• Journal of the Korean Operations Research and Management Science Society
• /
• v.24 no.3
• /
• pp.39-47
• /
• 1999

It has been concluded that the performance of class-based storage policy is better than the performance of random storage policy in the literature. However, the rack shortage problem assigned to the 1st class items makes the decision hard to apply the class-based storage policy in practice. In this paper, a new common zone concept is introduced between two classes to resolve the problem with class-based storage policy. The common zone is the area to accept items from both classes. An AS/RS model is developed for computer simulation study and the effect of common area sizes with various AS/RS operation conditions is analyzed.

• Proceedings of the Korean Operations and Management Science Society Conference
• /
• 2000.04a
• /
• pp.253-256
• /
• 2000

In general, Automated Storage/Retrieval Systems (AS/RS ) have racks of equal sized cells to utilize the concept of unit-load. Most of the techniques for the performance estimation of a unit-load AS/RS are a static model or computer simulation. Especially, their models were developed under assumption that the Storage/Retrieval (S/R) machine performs only single command (SC) or dual command (DC). In reality, defending on the operating policy and the status of the system at a particular time, the S/B machine performs a SC or a DC, or becomes .: idle. In order to resolve these weak points, we propose a stochastic model for the performance estimation of unit-load AS/RS by using a single-server queueing model. Expected numbers of waiting storage and retrieval commands are found

• Proceedings of the Korean Operations and Management Science Society Conference
• /
• 1995.04a
• /
• pp.956-957
• /
• 1995

대부분의 AS/RS 운영정책 연구는 등급별 저장이 임의저장보다 우수한 수행도를 나타낸다고 결론짓고 있다. 그러나 실제 현장에서는 임의 저장이나 이와 유사한 형태의 근거리 저장이 주로 사용되고 있는 실정이다. 이와 같이 AS/RS 운영에 관련된 연구결과들이 현실에서 적용되지 못하고 있는 데는 여러 가지 이유가 있겠지만 본 연구에서는 빈번하게 바뀌고 있는 생산계획에 그 원인을 두었다. 신제품의 투입으로 기존 제품의 생산량에 변화가 일어나는 경우가 빈번한 생산현장을 감안하여 GPSS로 AS/RS의 모형을 구축하고 그 운용상태를 파악해 볼 수 있도록 임의저장, 2등급저장, 3등급저장 등의 경우를 시험.분석하였다. 각 경우별로 적정 허용 변동율과 최대 허용 변동율, 변동에 대한 반응도 등이 분석되었다.

• Journal of Korean Society of Industrial and Systems Engineering
• /
• v.27 no.3
• /
• pp.26-30
• /
• 2004

We consider an AS/RS(Automated Storage/ Retrieval System) which performs both of single and dual commands. The service times of single and dual commands are assumed to be differently distributed. Steady-state probabilities at the service completion epochs are calculated. Then, we transform the arrival rates of storage and retrieval commands into those of single and dual commands. Using this, we propose a non-preemptive queueing model with priority for the performance analysis of an AS/RS, and obtain approximated means of queue lengths and waiting times.

• IE interfaces
• /
• v.3 no.2
• /
• pp.39-51
• /
• 1990

One of the most important and powerful tools available for design and/or study of the operation of complex systems and processes is simulation. Since automated material handling systems like AS/RS are often quite complex, a network-based simulation model is developed to analyze an automobile part supplier's automated storage and retrieval system(AS/RS). The network simulation model is implemented in the SLAM Ⅱ on a VAX 8800 computer. Performance of the AS/RS was tested for 3 dispatching rules, 3 work load levels, 2 storage policies, 3 levels of stacker crane break-down, and 2 conveyor system layouts. Results indicate that the AS/RS performance is primarily affected by the dispatching rule and work load level.

• Journal of Korean Society of Industrial and Systems Engineering
• /
• v.21 no.46
• /
• pp.137-146
• /
• 1998

Most studies related to AS/RS are about operation policies or system designs. However, the studies on operation policy could not deal with various products using the single unit load system. In this paper, two different sized units are introduced and six different AS/RS operation policies are developed to process the different sized items. Computer simulation is used to evaluate AS/RS performances on various operation policies and to find the most effective operation policy for each case. The simulation model is developed using GPSS/PC and operation policies include COL-FIFO-SP, SIL-FIFO-SP, COL-RN-SP, COL-FIFO-LP, SIL-FIFO-LP and COL-RN-LP. The policies are evaluated based on the criteria of crane utilization, rack utilization, crane moving time, throughput and waiting time.

• IE interfaces
• /
• v.12 no.2
• /
• pp.237-246
• /
• 1999

The Automated Storage/Retrieval System(AS/RS) is very expensive to be built. But there are few tools available which can be used to design the storage systems optimally considering overall system building costs and constraints. In this paper, we propose a design support system for optimal design of AS/RS which includes different sized cells using expert systems and mathematical models considering customer's demands. The optimization models are to be used to minimize the total cost including building, equipment and operation costs. Expert system technique is utilized for using the experiences of skilled designers. By using the proposed system, AS/RS can be designed accurately, economically and efficiently.

• Korean Journal of Microbiology
• /
• v.47 no.1
• /
• pp.14-21
• /
• 2011

Riboflavin synthase catalyzes the formation of one molecule of each riboflavin and 5-amino-6-ribitylamino-2,4-pyrimidinedione by the transfer of a 4-carbon moiety between two molecules of the substrates, 6,7-dimetyl-8-ribityllumazine. The most remarkable feature is the sequence similarity between the N-terminal half (1-97) and the C-terminal half domain (99-213). To investigate the structure and fluorescent characteristics of the N-terminal half of riboflavin synthase (N-RS) in Escherichia coli, more than 10 mutant genes coding for the mutated N-terminal domain of riboflavin synthase were generated by polymerase chain reaction. The genes coding for the proteins were inserted into pQE vector designed for easy purification of protein by 6X-His tagging system, expressed, and the proteins were purified. Almost all mutated N-terminal domain of riboflavin synthases bind to 6,7-dimethyl-8-ribityllumazine and riboflavin as fluorescent ligands. However, N-RS C47D and N-RS ET66,67DQ mutant proteins show colorless, indicating that fluorescent ligands were dissociated during purification. In addition, most mutated proteins show low fluorescent intensity comparing to N-RS wild type, whereas N-RS C48S posses stronger fluorescent intensity than that of wild type protein. Based on this result, N-RS C48S can be used as the tool for high throughput screening system for searching for the compound with inhibitory effect for the riboflavin synthase.