• Journal of Acupuncture Research
• /
• 제19권6호
• /
• pp.49-60
• /
• 2002

Objective : The aim of this experiment is to investigate the effect of acupuncture and moxibustion at SP6(Sanyinjiao) and BL28(Pangguanshu) on uranyl acetate-induced acute renal failure in rats. Methods : To induce experimental acute renal failure, uranyl acetate was intraperitoneally injected to rats by 5mg/kg. 3 days later acupuncture and moxibustion treatment was done at SP6(Sanyinjiao) and BL28(Pangguanshu) bilaterally of the rats. And then 4 days later, we measured rats' body weight, kidney weight, serum BUN, creatinine and serum eletrolyte levels(Na, K and Cl). Results : In the moxibustion group, hypertrophy index of kidney and serum BUN level were decreased marginally significantly, and serum creatinine, K levels were decreased significantly. But in the acupuncture group, there was no significance at this experiment. Conclusions : SP6(Sanyinjiao) and BL28(Pangguanshu) moxibustion can decrease serum BUN, creatinine, and serum eletrolyte levels on uranyl acetate-induced acute renal failure in rats. And the study will be done continuously about non-effect in the acupuncture group at this experiment.

• 대한한의학방제학회지
• /
• 제29권1호
• /
• pp.33-44
• /
• 2021

Renal ischemia-reperfusion injury(IRI), an important cause of acute renal failure (ARF), cause increased renal tubular injury. Jesaeng-sinkihwan (JSH) was recorded in a traditional Chines medical book named "Bangyakhappyeon (方藥合編)". JSH has been used for treatment of diabetes and glomerulonephritis with patients. Here we investigate the effects of Jesaeng-sinkihwan (JSH) in a mouse model of ischemic acute kidney injury. The animals model were divided into four groups at the age of 8 weeks; sham group: C57BL6 male mice (n=9), I/R group: C57BL6 male mice with I/R surgery (n=9), JSH Low group: C57BL6 male mice with surgery + JSH 100 mg/kg/day (n=9) and JSH High group: C57BL6 male mice with surgery + JSH 300 mg/kg/day (n=9). Ischemia was induced by clamping the both renal arteries during 25 min, and reperfusion was followed. Mouse were orally given with JSH (100 and 300 mg/kg/day during 3 days after surgery. Treatment with JSH significantly ameliorates creatinine clearance(Ccr), Creatinine (Cr) and blood urea nitrogen(BUN) in obtained plasma. . Treatment with JSH reduced kidney inflammation markers such as Neutrophil Gelatinase Associated Lipocalin (NGAL) and kidney injury molecule-1 (KIM-1). JSH also reduced the periodic acid schiff (PAS) staining intensity and picro sirius red staining intensity in kidney of I/R group. These findings suggest that JSH ameliorates tubular injury including renal dysfunction in I/R induced ARF mouse.

• Biomolecules & Therapeutics
• /
• 제25권1호
• /
• pp.26-43
• /
• 2017

Endocytosis is a process by which cells absorb extracellular materials via the inward budding of vesicles formed from the plasma membrane. Receptor-mediated endocytosis is a highly selective process where receptors with specific binding sites for extracellular molecules internalize via vesicles. G protein-coupled receptors (GPCRs) are the largest single family of plasma-membrane receptors with more than 1000 family members. But the molecular mechanisms involved in the regulation of GPCRs are believed to be highly conserved. For example, receptor phosphorylation in collaboration with ${\beta}$-arrestins plays major roles in desensitization and endocytosis of most GPCRs. Nevertheless, a number of subsequent studies showed that GPCR regulation, such as that by endocytosis, occurs through various pathways with a multitude of cellular components and processes. This review focused on i) functional interactions between homologous and heterologous pathways, ii) methodologies applied for determining receptor endocytosis, iii) experimental tools to determine specific endocytic routes, iv) roles of small guanosine triphosphate-binding proteins in GPCR endocytosis, and v) role of post-translational modification of the receptors in endocytosis.

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2004년도 학술발표회
• /
• pp.1162-1166
• /
• 2004

본 연구에서는 미국의 Environmental Modeling Research Laboratory(EMRL)에서 개발한 지리정보시스템과 수문유출모형이 접목된 WMS(Watershed Modeling System) Ver.6.1 모형을 HEC-1모형과 연계하여 한강수계 안성천의 제1지류인 한천의 유출 해석을 실시하였다. 이를 위해 유역내의 지형특성인자 추출 및 하천망 구성(WMS), 재현기간별 지속시간별 확률강우량 산정(FARD2002), Huff분포법을 이용한 시간분포, ARF(Area Reduction Factor)적용, HEC-1내의 SCS단위도법, Snyder 단위도법, Clark의 유역추적법에 포함된 각각의 매개변수의 최적화를 시도하여 분석하고, 설계 홍수량 산정시 이용될 수 있는 지침 마련을 목적으로 하였다.

• 한국방재학회 논문집
• /
• 제10권2호
• /
• pp.143-149
• /
• 2010

하천기본계획은 10년마다 재수립되고 있다. 경기도 지역의 중 소하천 중 최근 3년간 하천기본계획이 재수립 된 62개 중 소하천에 대하여 유역의 특성을 조사하고, 과거와 현재의 계획홍수량 및 계획강우량을 비교 분석하였다. 계획강우량이 감소한 유역 중에는 계획홍수량이 증가한 유역이 존재하였고, 이를 홍수량 재산정시의 유역자료 및 산정방법의 변화가 영향을 주었을 것으로 판단하고, 건건천 등 6개 유역을 대상으로 유역자료 및 산정방법의 변화에 따른 홍수량의 변화를 분석하였다. 분석결과, 계획홍수량의 증가에 영향을 미치는 인자는 임계지속시간과 임의시간의 적용 그리고 유출곡선지수(CN)증가로 나타났고, 감소에 영향을 미치는 인자는 강우 시간분포형 중 Huff의 4분위법 적용과 면적감소계수(ARF)의 적용이었다. 계획홍수량 산정 시 임계지속시간 적용은 홍수량을 평균 60% 증가시키는 것으로 나타났고, 강우의 시간분포형을 Mononobe방법에서 Huff의 4분위법으로 하였을 때 평균 62%정도 홍수량을 감소시키는 것으로 분석되어 임계지속시간 적용과 강우 분포형의 변화가 홍수량 변화에 가장 큰 영향을 미치는 것으로 나타났다. 그러나 영향인자들에 의한 홍수량 변화 경향이 유역에 따라 서로 다르게 분석되어 영향인자들과 홍수량 변화 사이에는 상관성이 확인되지 않았다. 이는 유역별로 지형, 기상, 수문수리학적 특성치가 상이하기 때문으로, 결국 홍수량 변화는 이러한 인자들의 복합적 작용에 의해 변화하게 되므로 특정 하천지점에서 계획홍수량을 채택할 경우 보다 합리적인 방법들로 재 산정된 홍수량을 채택하는 것이 바람직하다고 판단된다.

• 한국미생물·생명공학회지
• /
• 제23권4호
• /
• pp.446-453
• /
• 1995

$\alpha $-Arabinofuranosidase was produced by E. coli HB101 haboring the recombinant plasmid pKMG11 which contained the arfI gene of Bacillus stearothermophilus. The maximum production of the enzyme was observed when E. coli HB101 cells were grown at 37$\circ$C for 20 hours in the medium containing 0.5% arabinose, 1.0% tryptone, 0.5% yeast extract, and 1% NaCl. The $\ALPHA $-arabinofuranosidase produced was purified to homogeneity using a combination of 20-50% ammonium sulfate precipitation, DEAE-Sepharose CL-6B ion exchange column chromatography and Sepharose 6B-100 gel filtration. The purified enzyme was most active at 55$\circ$C and pH 6.5. The K$_{m}$ and V$_{max}$ values of the enzyme on $\rho $-nitrophenyl-$\alpha $-arabinofuranoside was determined to be 2.99 mM and 0.43 $\mu $mole/min (319.74 $\mu $mole/min/mg), respectively. The pI value was 4.5. The molecular weight of the native protein was estimated to be 289 kDa. The SDS-polyacrylamide gel clectrophoresis analysis suggested that the functional protein was a trimer of the 108 kDa identical subunits. The N-terminal amino acid sequence of the a-arabinofuranosidase was identified as X-Ser-Thr-Ala-Pro-Arg( \ulcorner )-Ala-Thr-Met-Val-Ile-Asp-X-Ala-Phe.

• Korean Journal of Acupuncture
• /
• 제20권2호
• /
• pp.55-66
• /
• 2003

Objectives : The aim of this experiment is to investigate the effects of moxibustion on SP6, Sp9 and BL28 in uranyl acetate-induced acute renal failure in rats. Methods : To induce experimental acute renal failure, uranyl acetate was intraperitoneally injected to rats by 5mg/kg. 3 days later moxibustion treatment was done at SP6, Sp9 and BL28 bilaterally of the rats. And then 4 days later, we measured rats' body weight, kidney weight, serum BUN, creatinine and serum electrolytic levels(Na, K and Cl). Results : In the SP6 group, serum BUN were significantly decreased (p<0.01). In the BL28 group, serum Na level were significantly decreased (p<0.05). In the $SP6{\cdot}SP9$ group, serum creatinine level were significantly increased but serum Na level, serum Cl level were significantly decreased(p<0.05). In the $SP6{\cdot}BL28$ group, hypertrophy index of kidney and serum creatinine level were significantly decreased(p<0.01) and serum BUN were marginally significantly decreased (0.1

• 한국임상수의학회지
• /
• 제30권1호
• /
• pp.57-60
• /
• 2013

개에서 포도중독에 의한 신부전 치료결과에 대한 임상적 분석을 위하여 205-2008년 기간 중 강원대학교 수의과대학 동물병원에 내원한 환자의 의료기록부를 분석하였다. 총 11두 (암컷 8두) 중 4두는 완전히 회복하였고, 3두는 폐사, 4두는 안락사로 처리되었다. 1두를 제외한 모든 환자는 포도를 섭취하였으나 정확한 섭취량은 알 수 없었다. 환자의 평균 연령은 5.3세 (범위 0.2-11.3세), 체중은 4.1 kg (범위 1.4-13 kg)였으며, 평균 입원기간은 7.1일 (범위 2-22일)로 나타났다. 모든 환자에서 구토와 식욕부진 증상을 보였으며, 일부 환자는 설사 (4두), 핍뇨 (5두), 무뇨 (4두) 소견을 보였다. 모든 환자에서 혈청 phosphorous, creatinine, BUN 농도가 증가된 소견을 보였으며, 고칼슘혈증 2두, 저칼슘혈증 2두, 나머지 7두는 정상소견이었다. 혈청 농도가 증가한 항목은 amylase 8두, ALP 7두, ALT 5두였으며, 혈액 가스 분석에서 8두는 대사성산증을 보였다. 경미하거나 중등도의 빈혈 소견이 5두에서 관찰되었으며, 회복한 환자와 비교할 때 폐사축의 경우 혈소판과 림프구 수가 상대적으로 낮았다.

• 대한한방내과학회지
• /
• 제32권2호
• /
• pp.278-287
• /
• 2011

Objectives : The object of this study was to observe the nephroprotective effects of Sojongchobisunki-tang (SCST), which has traditionally been used as Korean medicine for treating various renal diseases, on cisplatin-induced rat acute renal failure. Methods : Three different dosages of SCST were orally administered once a day for 23 days before cisplatin treatment (5 mg/kg, single intraperitoneally administered) and 5 days after cisplatin treatment (once a day for 28 days). 6 groups, of 8 rats per group were used in the present study after 7 days of acclimatization. Changes of the body weight, kidney weight, serum BUN and creatinine levels were observed, as well as changes of the kidney MDA and GSH contents. The results were compared with captopril 100 mg/kg of which the effects on cisplatin-induced acute renal failures are already confirmed. Results : Acute renal failure induced by cisplatin were induced by oxidative stress and related lipid peroxidation in the present study. However, these acute renal failures and inhibition of antioxidant effects induced by cisplatin were dose-dependently reduced by treatment at all three different dosages of SCST extracts. Conclusions : This study suggests that SCST extracts showed favorable effects on the cisplatin-induced rat ARF.

• 조명전기설비학회논문지
• /
• 제15권6호
• /
• pp.8-14
• /
• 2001

메탈핼라이드램프는 고효율, 고연색성이며 집광능력이 좋다. 그러나 메탈핼라이드 램프의 단점은 아크튜브내에서 음향공명현상이 발생되는 것으로 알려져 있다. 이러한 음향공명은 점등상태의 불안정과 기체의 심한 파동을 일으킨다. 심한 파동과 불안정한 점등으로 아크가 소멸되는 부위는 전압이 상승되고, 방전관내벽에 근접된 아크는 국부의 과열로 튜브를 금가게한다. 이 연구의 목적은 펄스스타트 메탈핼라이드램프 MH200[W]의 아크튜브내에서 음향공명을 감소시키는 것이다. 음향공명현상을 감소시키기 위하여 전자식안정기가 84.6[kHz]에서 94.2[kHz] 대역내에 있는 89[kHz]의 단독주파수인 정현고주파를 램프에 공급하기 위하여 설계되었다. 실험결과 고효율 펄스스타트 MH200[W] 램프의 방전관내에 음향공명 현상이 없었다.