• 아시안잔디학회지
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• 제6권2호
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• pp.73-81
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• 1992

잔디(Zoysia japonica)의 1차 생산성과 광조건과 상호작용을 평가하고 생리생태학적인 특성을 규명하기 위하여 차광을 조절한 상태에서 인공군락의 물질생산과 성장해석에 미치는 영향에 대하여 분석하였다. 그 결과의 요약은 다음과 같이 하였다. 1. RGR은 각 시험구 공히 이식후 20일경까지의 생장과정에서 높게 나타나고 이후 생장이 진행됨에 따라 낮아지는 경향을 보였다. 차광율이 높고 광조사 시간이 짧을수록 이식후 초기생장부터 현저히 낮은 치를 보였다. 2. NAR은 이식후 초기생장과정에서 높은치를 보였고 이후 생장이 진행됨에 따라 낮은 치를 나타내었으며 차광율이 높고 광조사 시간이 짧을수록 현저히 낮은 치를 보였다. 3. LAR은 Control구와 30% 차광구에서 이식후 초기생장과정에서 증가율이 높게 나타났으나 50% 및 70% 차광에서는 이식후 40일 이후부터 높게 나타나는 경향을 보였다. LAR은 RGR에 대하여 역의 상관관계를 나타내었다. 4. CGR은 각 공히 이식후 50일까지는 생장이 낮아지는 경향을 보였다. LAI와는 정의 상관을 나타내었고 최적엽면적 지수는 Control구와 30%, 50% 및 70% 차광구에서 각각 2.10, 1.75, 1.25, 0.90으로 나타났다.

• Journal of Periodontal and Implant Science
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• 제33권1호
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• pp.91-102
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• 2003

Several effective treatment methods and materials have been developed for the treatment of furcation involvement. Currently, the combination of guided tissue regeneration (GTR) and bone grafts is the most commonly prescribed method of treating furcation involved defects. But because these cases often present with poor accessibility, placement of the membrane may be difficult and consequently, clinically impractical. In this study, the alveolar bone healing patterns of adult beagle dogs presenting with alveolar bone destruction treated by one of two methods - treatment using solely bone allografts (BBP(R)), or treatment using bone allografts (BBP(R)) stabilized by a fibrin adhesive - were comp ared. The effects of the fibrin adhesive on the initial stabilization of the newly formed bone, subsequent regeneration of bone, and the feasibility of the clinical application of the fibrin adhesive were analyzed. The results of the study were as follows: 1. Clinical signs of inflammation at the 4-8 week interval were not observed: but signs of mild inflammation were histologically observed at the 4-week interval. 2. Allografts stabilized by fibrin adhesive showed good bone formation, whereas defects treated with only the allograft material showed incomplete alveolar bone regeneration. 3. Allografts stabilized by fibrin adhesive showed a decrease in the amount old bone with a concurrent increase in the formation of new lamellar bone four weeks post-op, whereas defects treated with only the allograft material showed no new lamellar bone formation at the same interval. 4. In detects treated with only the allograft material, the defective area was filled with connective tissue 8-weeks post-op, whereas fibrin adhesive stabilized allografts showed viable connections between the original bone and the newly formed bone, in addition to neovascularization 8-weeks post-op. The results of this study show that concurrent use of fibrin adhesive materials can stabilize the allograft material and aid in new bone formation Although the stability of fibrin adhesives fall short of the results achievable by GTR membranes, in cases presenting with poor accessibility that contraindicate the use of membranes, fibrin adhesive materials provide a viable and effective alternative to graft stabilization and new bone formation.

• 한국가축번식학회지
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• 제4권1호
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• pp.47-74
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• 1980

The present experiments were carried out to elucidate the effects of hypophysectomy and subsequent administration of sex hormone on thyroid, adrenal gland, gonads and blood plasma components in the rat. The jresults obtained were summarized as follows: The weight of the thyroid gland of both male and female hypophysectiomzed rats decreased markedly from 7 days up to 56 days after the hypophysectomy as compared to the control group. The administration of sex hormone (6 mg of testosterone propionate to male and 6 mg of hexestrol to female) to the hypophysectomized rat gave on effect on the change in the weight of the thyroid gland. The hopophysectomy decreased the uptake of radioactive iodine in the thyroid gland in both male and female rats with time. Subsequent administration of the sex hormone caused no effect. With regard to the histological changes of the thyroid gland, the hypophysectomy caused significant changes in the gland showing a remarkable degeneration. The function of the gland seemed to disa, pp.ar almost completely on 56th day after the hypophysectomy. Upon the administration of sex hormone after the hypophysectomy, however. the epithelia of the follicle which has changed to flat from has partly returned to its functional cubicfrom and nuclei recovered as nearly as normal. These recovery were more remarkable in the female than in the male. The hypophysectomy kept causing a significant decrease in the weight of the adrenal gland in male and female rats during the period of observation (up to 56 days) as in the case of thyroid gland. The administration of sex hormone has on effect in this respect either. The hypophysectomy also caused a marked morphological change in the gland: zona fasciculata and zona reticularis were dicreased in size quichly after the hypophysectomy. The administraton of the sex hormone to the hypophysectomized rat resulted in clear distinction among the three layers of the adrenal cortex which otherwise very diffused. In the male, this phenomenum was more remarkable than in the female and the pattern of the cell arrangements and the thickening of each layer became similar to those of normal rats. The gonads of both sexes have also kept decreasing in the weight and degenerated in morpohology after the hypophysectomy. However, the degenerate follicle became enlarged after the administration of hexestrol in the female. Furthermore, the vacuoles found in interstitial cells of hypophysectomized rat disa, pp.ared after the administration of testosterone in the male and the formation of spermatocytes seemed to be recovered. Hypophysectomy also caused a gradual increase in the contents of total protein, non-protein nitrogen, total lipid, cholesterol and calcium in the blood plasma with time. The concentrations of sodium, potassium and chloride in the blood did not change after the hypophysectomy. Sex hormone caused practically no change in above tendency.

• 한국가축번식학회지
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• 제21권2호
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• pp.139-146
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• 1997

소와 돼지의 난관 상피세포가 마우스 초기배의 발달 에 미치는 영향과 체외배양에 있어 최적의 배양조건을 알아보기 위하여 ICR 계통의 마우스에 PMSG 7.5 IU와 hCG 7.5 IU를 각각 복강주사하고 자연교미하여 48시간 경과 후에 난관에서 2-세포 초기배를 D-PBS로 관류하여 회수하였다. 회수된 배아는 소와 돼지의 난관 상피세포와 공배양하여 그 효사를 배반포 발달율과 핵의 수를 조사하였다. 또한 생체내와 실험 관내의 발육상태를 비교하기 위하여 hCG접종후 120 시간동안 생체내에서 발육시킨 신선 배반포배를 자궁 에서 채취하여 그 핵수를 계산하였다. 마우스 초기배는 TCM 199, Ham's F-10, Medicult IVF 배양액에서 소 난관 상피세포 또는 돼지 난관 상피세포와 공배양할 경우 91-97%의 높은 배반포 발달율을 보였으며 난관 상피세포간의 차이는 나타 나지 않았다. 각 배양조건에 따라 배양된 배반포의 핵수는 체내에서 자란 배반포에 비해 체외에서 배양한 배반포에서 유의적으로 적었다. 체외배양중 핵수는 공배양하지 않은 TCM 199, Ham's F-10, Medicult IVF medium 에서 각각 68.1${\pm}$6.00, 67.3${\pm}$4.49, 66.4${\pm}$5.64개로 나타났으며 BOEC와 공배양하였을 경우에는 94.3${\pm}$8.61, 92.5${\pm}$7.60, 92.1${\pm}$6.107B, POEC와 공배양하였을 때는 93.3${\pm}$5.80, 92.9${\pm}$6.53, 92.3${\pm}$7.35개로 체내에서 배양된 배반포의 경우의 107.2${\pm}$7.43개보다 적었다. 이상의 결과로 난관 상피세포인 BOEC와 POEC는 마우스 초기배야와의 체외공배양시 배아의 발달과 분화에 이로운 영향을 주어 발달율과 부화율를 향상시키나 핵수 증가에서는 체내조건보다 미홉한 것으로 사료된다.

• 한국가축번식학회지
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• 제21권2호
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• pp.147-156
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• 1997

Ultrasound-guided follicular aspiration was performed in Holstein heifers once weekly with or without pretreatment of single or multiple decreasing doses using a total of 400 mg FSH. Oocytes were aspirated with a 6.5 MHz convex-array ultrasound trasducer designed for intravaginal use. All the visible follicles larger than 4 mm in diameter were punctured with a 17 gauge, 55 cm needle at each aspiration session and the follicular fluids containing oocytes were obtained by vacuum suction. The results obtained were as follows: As a preliminary experiment, the recovery rates of folicular oocytes by ultrasound-guided aspiration from the isolated ovaries of Korean native cows were compared between suction methods using manual syringe or vacuum pump. The recovery rate of oocytes using vacuum pump (80.7%) was significantly (P<0.05) higher than that using manual syringe (47.1%). The follicles were counted by their size in diameter with ultrasound image, and recovery rates and grades of follicular oocytes collected by ultrasound-guided aspiration were investigated in Holstein heifers pretreated with or without FSH. A group of heifiers were injected with multiple decreasing doses (twice a day for 3 days) of a total of 400 mg FSH. The other 2 groups were injected with a single dose of 400 mg FSH mixed with 25% PVP. Ultrasound observation of follicle population and/or ultrasound-guided transvaginal oocyte aspiration were performed 12 hrs following the last FSH injection in the multiple dose group, and 48 or 60 hrs after FSH injection in the single dose groups. Most of the visible follicles had small size of less than 3 mm in diameter in unstimulated heifers (71.0%), but medium size in all the heifers treated with FSH. (70.5 to 92.8%). The number of OPU follicles per session (4.6$\pm$1.9) were much less, compared to the vilsible follicle counts (9.7$\pm$2.2), in the nustimulated heifers due to the small dominant follicles. Among 4 goups of heifers the most visible as well as OPU follicles were observed in the heifers at 60 hrs following treatment of a single dose of 400 mg FSH (21.2$\pm$2.3 and 21.0$\pm$2.0), and the differences in both the follicle counts between the groups was found significant (P<0.05) The rates of oocyte recovery from the follicles by ultrasound-guilded aspiration were varied 46.3 to 75.0% in the heifers unstimulated and treated with a single dose of 400 mg FSH, but the group difference was not significant. The number of recovered oocytes per session a, pp.ared to be highest at aspiration at 60 hrs following single FSH (10.6$\pm$2.2) than at aspiration at 48 hrs after single FSH (7.8$\pm$2.7) or in the unstimulated heifers (3.4$\pm$3.0). The proportion of grade I and II oocytes to all oocytes collected was varied 31.8 to 64.0% between the groups. However, there was found no significant difference in both the number of oocytes recovered per session and the percentage and the percentage of grade I and II oocytes. From the above results it was concluded that the more oocytes of superior quality might be recovered economically by ultrasound-guided aspiration at 60 hrs following the pretreatment of a single dose of 400 mg FSH and by suction using a vacuum pump system of about negative pressure of 75 to 85 mmHg.

• 한국잡초학회지
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• 제9권3호
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• pp.201-213
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• 1989

제주도(濟州道)의 농경지(農耕地)와 농경지(農耕地) 주변(周邊)에 발생(發生)하는 하계잡초(夏季雜草)를 조사(調査)하여 얻어진 결과(結果)를 요약(要約)해 보면 다음과 같다. 1. 조사(調査)된 총(總) 잡초종(雜草種) 수(數)는 54과(科) 233여종(餘種)이었으며 국화과(菊花科)가 41종(種)으로 가장 많고 벼과(科) 28종(種), 꿀풀과(科)14종(種), 콩과(科) 13종(種), 마디풀과科) 12종순(種順)으로 많이 발생(發生)하였다. 2. 전작지(田作地)에서는 39과(科) 126종(種)이 조사(調査)되었으며, 여우구슬, 제주진득찰 등(等)의 군락(群落)과 바랭, 주깨풀 등(等)의 군락(群落)이 형성(形成)되어 있었고 쇠비름, 바랭이, 방동사니 순(順)으로 우점(優占)하고 있었다. 3. 전작지(田作地)에서는 20과(科)38종(種)이 조사(調査)되었으며 미나리, 쇠털골의 군락(群落)이 형성(形成)되어 있었고, 물달개비, 방동사니, 여뀌 순(順)으로 우점(優占)하고 있었다. 4. 과수원(果樹園)에서는 36과(科) 108종(種)이 조사(調査)되었으며 쇠별꽃, 제비꽃 등(等)의 군락(群落)과 쥐꼬리망초, 외풀 등(等)의 군락(群落)이 형성(形成)뇌어 있었고, 우점도(優占度)는 쇠비름, 바랭이, 석류풀 순(順)으로 높았다. 5. 농경지(農耕地) 주변(周邊)에서는 50과(科) 214종(種)의 잡초(雜草)가 조사(調査)되었으며 새콩, 왕고들빼기 등(等)의 군락(群落)과 사위질빵, 갯쭉부쟁이 등(等)의 군락(群落)이 형성(形成)되어 있었고, 바랭이, 쑥, 쇠비름 순(順)로 우점도(優占度)가 높았다.