Korean Journal of Animal Reproduction (한국가축번식학회지)
- Volume 21 Issue 2
- /
- Pages.147-156
- /
- 1997
- /
- 1226-5284(pISSN)
Effect of FSH Pretreatment on Follicle Development and Oocyte Recovery by Ultrasound-guided Aspiration in Holstein Heifers
젖소의 초음파 유도 채란율에 대한 FSH 전처리 효과의 비교
- Park, Chung-Saeng (Dept.of Animal Science,Gyeongsang National University) ;
- Jo, Seong-Geun (Dept.of Animal Science,Gyeongsang National University) ;
- Lee, Jeong-Gyu ;
- Gang, Tae-Yeong ;
- Park, Seong-Jae ;
- Gong, Il-Geun ;
- Choe, Min-Cheol
- 박충생 (경상대학교 축산학과) ;
- 조성근 (경상대학교 축산학과) ;
- 이정규 (경상대학교 수의학과) ;
- 강태영 (농촌진흥청 축산기술연구소 남원지소) ;
- 박성재 (경상대학교 축산진흥연구소) ;
- 공일근 (경상대학교 수의학과) ;
- 최민철
- Published : 1997.06.01
Abstract
Ultrasound-guided follicular aspiration was performed in Holstein heifers once weekly with or without pretreatment of single or multiple decreasing doses using a total of 400 mg FSH. Oocytes were aspirated with a 6.5 MHz convex-array ultrasound trasducer designed for intravaginal use. All the visible follicles larger than 4 mm in diameter were punctured with a 17 gauge, 55 cm needle at each aspiration session and the follicular fluids containing oocytes were obtained by vacuum suction. The results obtained were as follows: As a preliminary experiment, the recovery rates of folicular oocytes by ultrasound-guided aspiration from the isolated ovaries of Korean native cows were compared between suction methods using manual syringe or vacuum pump. The recovery rate of oocytes using vacuum pump (80.7%) was significantly (P<0.05) higher than that using manual syringe (47.1%). The follicles were counted by their size in diameter with ultrasound image, and recovery rates and grades of follicular oocytes collected by ultrasound-guided aspiration were investigated in Holstein heifers pretreated with or without FSH. A group of heifiers were injected with multiple decreasing doses (twice a day for 3 days) of a total of 400 mg FSH. The other 2 groups were injected with a single dose of 400 mg FSH mixed with 25% PVP. Ultrasound observation of follicle population and/or ultrasound-guided transvaginal oocyte aspiration were performed 12 hrs following the last FSH injection in the multiple dose group, and 48 or 60 hrs after FSH injection in the single dose groups. Most of the visible follicles had small size of less than 3 mm in diameter in unstimulated heifers (71.0%), but medium size in all the heifers treated with FSH. (70.5 to 92.8%). The number of OPU follicles per session (4.6