• 에너지공학
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• 제25권1호
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• pp.43-47
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• 2016

한수원 중앙연구원은 2007년부터 정부과제의 하나로 전기출력이 1500MWe급인 GEN III+ 원전 APR+를 개발해 왔다. APR1400 보다 안전성이 진전된 노형을 개발하기 위해 국내외에서 건설되거나 설계중인 ALWR의 개선된 설계특성을 조사하였다. 국내외의 원전건설 사업에 적합한 APR+ 표준설계를 개발하기 위해 신개념설계특성과 후쿠시마 원전사고 경험을 설계에 반영하였다. APR+의 안전성향상 표준설계 단계(2013.1 ~ 2015.12)에서 한 번의 경제성평가를 수행하였다. 설계 안전성향상 기술개발 단계에의 경제성 평가 결과 APR+ N-th호기는 국내석 탄화력 1000MWe급 대비 39.2% 경쟁력 우위인 것으로 평가되었다. 또한 APR+원전은 해외 원전 선진국 ALWR에 비해 동등 이상 수준의 경쟁력을 확보하는 것으로 평가되었다.

• Journal of Microbiology and Biotechnology
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• 제32권6호
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• pp.800-807
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• 2022

Four aprE genes encoding alkaline serine proteases from B. subtilis strains were used as template genes for family gene shuffling. Shuffled genes obtained by DNase I digestion followed by consecutive primerless and regular PCR reactions were ligated with pHY300PLK, an E. coli-Bacillus shuttle vector. The ligation mixture was introduced into B. subtilis WB600 and one transformant (FSM4) showed higher fibrinolytic activity. DNA sequencing confirmed that the shuffled gene (aprEFSM4) consisted of DNA mostly originated from either aprEJS2 or aprE176 in addition to some DNA from either aprE3-5 or aprESJ4. Mature AprEFSM4 (275 amino acids) was different from mature AprEJS2 in 4 amino acids and mature AprE176 in 2 amino acids. aprEFSM4 was overexpressed in E. coli BL21 (DE3) by using pET26b(+) and recombinant AprEFSM4 was purified. The optimal temperature and pH of AprEFSM4 were similar to those of parental enzymes. However, AprEFM4 showed better thermostability and fibrinogen hydrolytic activity than the parental enzymes. The results indicated that DNA shuffling could be used to improve fibrinolytic enzymes from Bacillus sp. for industrial applications.

• 에너지공학
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• 제23권4호
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• pp.236-239
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• 2014

한수원 중앙연구원은 2007년부터 정부과제의 하나로 전기출력이 1500MWe급인 GEN.III+ 원전 APR+를 개발하고 있다. APR1400에 비해 보다 개선된 안전성과 경제성을 갖는 원전을 개발하기 위해 국내 건설 중인 원전과 해외에서 개발 또는 건설 중인 ALWR(Advanced Light Water Reactor)의 설계내용 및 후쿠시마 원전사고로부터 도출된 개선사항을 반영하여 한국 실정에도 맞고, 해외 수출형 원전에도 부합되는 원전을 설계하고 있다. APR+의 경쟁력을 확인하기 위해 APR+ 표준설계개발 단계에서 3회의 경제성 평가를 수행하였다. 표준설계개발 단계에의 3차(최종) 경제성 평가 결과 APR+ N-th호기는 국내석탄화력 1000MWe급 대비 약 23% 경쟁력 우위인 것으로 평가되었다.

• Journal of Microbiology and Biotechnology
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• 제24권7호
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• pp.969-978
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• 2014

The aprE2 gene with its prosequence from Bacillus subtilis CH3-5 was overexpressed in Escherichia coli BL21(DE3) by using plasmid pET26b(+). After IPTG induction, active and mature AprE2 was produced when cells were grown at $20^{\circ}C$, whereas inactive and insoluble enzyme was produced in a large amount when cells were grown at $37^{\circ}C$. The insoluble fraction was resuspended with 6 M guanidine-HCl and dialyzed against 2 M Tris-HCl (pH 7.0) or 0.5 M sodium acetate (pH 7.0) buffer. Then active AprE2 was regenerated and purified by a Ni-NTA column. Purified AprE2 from the soluble fraction had a specific activity of $1,069.4{\pm}42.4U/mg$ protein, higher than that from the renatured insoluble fraction. However, more active AprE2 was obtained by renaturation of the insoluble fraction. AprE2 was most stable at pH 7 and $40^{\circ}C$, respectively. The fibrinolytic activity of AprE2 was inhibited by PMSF, but not by EDTA and metal ions. AprE2 degraded $A{\alpha}$ and $B{\beta}$ chains of fibrinogen quickly, but not the ${\gamma}$-chain. AprE2 exhibited the highest specificity for N-succinyl-Ala-Ala-Pro-Phe-pNA. The $K_m$ and $k_{cat}/K_m$ of AprE2 was 0.56 mM and $3.10{\times}10^4S^{-1}M^{-1}$, respectively.

• 한국안전학회지
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• 제31권6호
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• pp.129-134
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• 2016

Standard design of APR+(advanced power reactor plus) was certified at 2014 by Korea regulatory body. Based on the experience gained from OPR1000 and APR1400, the APR1400 was being developed as a 1,500MWe class reactor using Korean technologies for design code, reactor coolant pump, and man-machine interface system. APR+ has been basically designed to have the seismic design basis of safe shutdown earthquake (SSE) 0.3g, a 4-train safety concept based on N+2 design philosophy, and a passive auxiliary feedwater system (PAFS). Also, safety issues on the Fukushima-type accidents have been extensively reviewed and applied to enhance APR+ safety. APR+ provides higher reliability and safety against tsunami and earthquake. The purpose of this paper is to implement probabilistic safety assessment considering these design features and to analyze sensitivity of core damage frequency for large loss of coolant accident of APR+.

• 원자력산업
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• 제30권3호
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• pp.72-78
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• 2010

KEPCO는 APR1000 원전을 개발 중에 있다. APR1000은 2루프 1000MWe급의 가압경수로로, 국내에 운영중이고 건설중인 검증된 OPR1000 설계를 기반으로 하고 있다. APR1000은 원전 수요자의 요구에 응하기 위해 안전성, 신뢰성 및 경제성을 설계에 고려하여 개발 중에 있다. APR1000의 대표적인 설계 특성으로는 60년 수명, 0.3g 내진 설계, MMICs, 저온 덮개 원자로(Cold Head Reactor), 안전 주입 탱크 내의 피동형 유량 조절 장치 등이 있다. 본고에서는 APR1000의 설계 요건과 안전 관련 설게 특징을 소개하고자 한다.

• 생명과학회지
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• 제23권1호
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• pp.15-23
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• 2013

Bacillus amyloliquefaciens CH51은 분자량 27 kDa 크기의 subtilisin 타입의 혈전용해능을 지니는 단백질분해효소인 AprE51을 생산하였다. 이전연구에서 더 우수한 혈전용해 활성을 갖는 AprE51-6이 세포외 돌연변이법으로 생산되었으며, 본 연구에서는 이 개선된 효소인 AprE51-6의 열안정성을 증진시킬 목적으로 B. subtilis subtilisin E의 아미노산과의 상동성 분석을 통하여 두 아미노산인 Gly-166과 Asn-218이 치환되었다. 그 결과 G166R과 N218S 돌연변이체는 혈전용해능을 보이는 용해능 배지에서 원 효소보다 각각 1.8배와 4.5배 높은 혈전용해능을 보였다. 정제된 두 돌연변이효소인 AprE51-7과 AprE51-8는 원효소인 AprE51-6에 비하여 1.9 그리고 2.5배 높은 $k_{cat}$값을 나타내었고, 2.1과 1.9배 낮은 기질친화력을 나타내는 $K_m$값을 보여주었다. 특히 AprE51-8는 나토키나아제에 비하여 알칼리 pH 영역에서 높은활성을 유지하였고, $60^{\circ}C$에서 더 우수한 열안정성을 보여주었다. 열안정성의 정도를 나타내는 척도인 반감기 값에서도 AprE51-7과 AprE51-8는 $50^{\circ}C$에서 21.5분과 27.3분으로 기존의 AprE51보다 2배 그리고 2.6배 더 긴 반감기를 보였다.

• Journal of Acupuncture Research
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• 제23권3호
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• pp.191-206
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• 2006

Objective & Methods : The purpose of this study is to observe the effects of Angelicae Pubescentis Radix herbal-acupuncture solution(APR-HAS) at Joksamni(ST36) on collagen IT induced arthritis in DBA/1J mice. The author performed several experimental items to analyze several cytokines and immune cells related with RA. Results : 1. In the APR-HA group, the incidence of arthritis and arthritis index were significantly decreased. 2. In APR-HA group, the levels of IL-6, $INF-{\gamma}$, $TNF-{\alpha}$, IgG, IgM, $IL-{\beta}$ and Anti-collagen II in serum of the CIA mouse were significantly decreased. 3. In APR-HA group, the level of $IFN-{\gamma}$, IL-4 in the CIA mouse spleen cell culture were significantly decreased. 4. In histology, the cartilage destruction and synovial cell proliferation were decreased in the APR-HA group, and the collagen fiber expressions in the APR-HA group were similar with that of the Normal group. 5. In the APR-HA group, CD3e+/CD19+ and CD4+/CD8+ were similarly maintained as Normal group in the CIA mouse lymph nodes, 6. In the APR-HA group, CD3e+/CD69+ was significantly decreased in the CIA mouse joint. 7. In the APR-HA group, CD11a+/CD19+ and CD11b+/Gr-l+ were significantly decreased in the CIA mouse lymph nodes 8. In the APR-HA group, CD4+/CD25+ was decreased in the CIA mouse spleen cell. 9. In the APR-HA group, CD4+/CD25+ was similarly maintained as Normal group in the CIA mouse lymph nodes.

• Nuclear Engineering and Technology
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• 제41권8호
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• pp.995-1004
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• 2009

The Advanced Power Reactor 1400 (APR1400) is an evolutionary advanced light water reactor (ALWR) based on the Optimized Power Reactor 1000 (OPR1000), which is in operation in Korea. The APR1400 incorporates a variety of engineering improvements and operational experience to enhance safety, economics, and reliability. The advanced design features and improvements of the APR1400 design include a pilot operated safety relief valve (POSRV), a four-train safety injection system with direct vessel injection (DVI), a fluidic device (FD) in the safety injection tank, an in-containment refueling water storage tank (IRWST), an external reactor vessel cooling system, and an integrated head assembly (IHA). Development of the APR1400 started in 1992 and continued for ten years. The APR1400 design received design certification from the Korean nuclear regulatory body in May of2002. Currently, two construction projects for the APR1400 are in progress in Korea.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2014년도 추계학술대회 논문집
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• pp.399-401
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• 2014

The comprehensive vibration assessment program(CVAP) of APR+ nuclear power plant(NPP) is classified as non-prototype, category II with Palo Verde NPP as valid prototype. In this paper, CVAP for first plant of APR+ NPP is proposed. The Control Element Assembly(CEA) shroud of APR+ NPP is different from that of Palo Verde NPP. And the Core Support Barrel(CSB) outer diameter and the flow rate of normal operation of APR+ NPP are larger than those of Palo Verde NPP. Vibration and stress analysis program should be conducted to establish test acceptance criteria. Limited vibration measurement program should be implemented to establish the margin of safety, demonstrate the satisfaction of test acceptance criteria and confirm the similar vibratory response between the APR+ and Palo Verde NPP. Because of the change of normal operation condition, the nominal differences between APR+ and Palo Verde NPP in the structural and hydraulic analysis are studied to determine the measurement locations.