• The Korean Journal of Pesticide Science
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• v.12 no.4
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• pp.382-390
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• 2008

This study was performed to evaluate the acute toxicity and residual toxicity of the 69 kinds of agrochemicals (41 insecticides, 18 fungicides, and 10 acaricides) against honeybee, Apis mellifera and bumblebee, Bombus terrestris. According to the IOBC standard, the toxicity showed below 30% was classified as non-toxic. Among 41 insecticides, five insecticides (acetamiprid, chlorfenapyr, thiacloprid, milbemectin, and buprofezin+amitraz) against the honeybee; eight insecticides (methomyl, thiodicarb, acetamiprid, chlorfenapyr, thiacloprid, abamectin, spino sad, buprofezin+amitraz) against the bumblebee did not show any toxic effect. Therefore, it thought to being safe. Other 18 fungicides and 10 acaricides were safe against the honeybee and bumblebee. In residual toxicity against the honeybee, eight insecticides (dichlorvos, methomyl, imidachlorprid, emamectin benzoate, spinosad, cartap hydrochloride, chlorfenapyr, and endosulfan) among 41 insecticides tested were safe at three days after treatment; however, sixteen insecticides (dimethoate, fenitrothion, fenthion, methidathion, phenthoate, pyraclofos, fenpropathrin, clothianidin, dinotefuran, thiamethoxam, abamectin, acetamiprid+ethofenprox, acetamiprid+indoxacarb, bifenthrin+imidacloprid, ethofenprox+phenthoate, imidacloprid+methiocarb) still remain high toxicity at eleven days after treatment. Against the bumblebee, residual toxicity showed as safe in seven insecticides (dimethoate, methidation, a-cypermethion, ethofenprox, indoxcarb, chlorpyrifos+a-cypennethrin, esfenvalerate+fenitrochion) at three days after treatment; however, eight insecticides (fenitrothion, pyraclofos, clothianidin, fipronil, acetamiprid+ethofenprox, chlorpyrifos+bifenthrin, ethofenprox+phenthoate, imidacloprid+methiocarb) still showed high toxicity at seven days after treatment. From above results, it will be useful information to select insecticides being safe and effective against the honeybee and bumblebee.

• Journal of Food Hygiene and Safety
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• v.32 no.3
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• pp.228-233
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• 2017

The aim of the current study was to examine genotoxicological safety of purified bee venom (Apis mellifera L.) The bacterial reverse mutation in Salmonella typhimurium (TA100, TA1535, TA98, and TA1537) and Escherichia coli (WP2 uvrA) were evaluated with purified bee venom at concentrations of 0, 1.5, 5, 15, 50, 150, and $500{\mu}g/plate$. Purified bee venom was negative in Ames test with both in the presence and absence of rat liver microsomal enzyme. According to these results, we concluded that purified bee venom did not cause bacterial reverse mutation. The safety of the purified bee venom at practical doses needs to be further evaluated in in vivo genotoxicity assays.

• Korean Journal of Veterinary Research
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• v.16 no.1
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• pp.11-25
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• 1976

Studies on pathogenicities and developmental stages of Nosema apis (Zander, 1909) were carried out through artificial infection to Nosema free honey bees with various levels of spores isolated from local honey bee colony. The results obtained were summarized as follows: 1. The clinical symptoms were observed as dysentery, enteritis of mid-gut (enlargement and decoloration), crawling posture and shortening of the longevity of worker bees in the rearing honey bee colony inoculated with the spores. 2. Number of spores harvested from laboratory rearing honey bees were progresively increased to 4 weeks after inoculation. The regression equations and coefficients of correlations to various spore levels were as follows in each treatment colony. Colony 1. ($$1,000{\times}10^4spores/ml$$) $$y_{c1}=471{\times}10^{4}x+454{\times}10^4(r=0.65^*$$) Colony 2. ($$500{\times}10^4spores/ml$$) $$y_{c2}=340{\times}10^{4}x+207.8{\times}10^4(r=0.99^{**}$$) Colony 3. ($$100{\times}10^4spores/ml$$) $$y_{c3}=150{\times}10^{4}x+84.2{\times}10^4(r=0.99^{**}$$) Colony 4. ($$10{\times}10^4spores/ml$$) $$y_{c4}=13.8{\times}10^{4}x+13{\times}10^4(r=0.98^{**}$$) 3. Average longevity of worker bees artificially infected with Nosema apis was shortened as 21.7~43.8% compare to the control. (p<.05, p<.01) 4. The spores which were isolated from honey bee colony infected with Nosema disease were ovoid or spherical form, and measured, as a rule, from $4.7{\mu}m$ to $6.1{\mu}m$ (mean $5.3{\mu}m$) in length and from $2.4{\mu}m$ to $3.2{\mu}m$ (mean $2.9{\mu}m$) in width. 5. In the mid-gut of honey bees, the spore was progresively germinated and became trophozoite stage. The trophozoites were grown to meronts and their binary fission were begun. The divided two sporoblasts were developed to the spores which had elastic membrane. The new spores were shed in excreta of honey bees 10~15 day after inoculation at $25{\pm}2$ centigrade. 6. The ultrastructure of spore membrane consisted of three layers, such as, outer, middle and inner layer. The sporoplasm consisting lamellar structure occupied only anterior part of the spore and was often extended to posterior direction where definite vacuoles and a polar filament was able to detect.

• Applied Biological Chemistry
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• v.31 no.3
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• pp.241-248
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• 1988

In order to determine the approptiate usage of insecticides to honeybee(Apis mellifera L.), median effective dose to seven insecticides were studied. $LC_(50)$ value of DDT was the highest as being 58 ppm, and that of EPN was the lowest as being 1.61ppm. Various detoxifying enzymes from the midget cf adult worker bee, including microsomal oxidases, glutathione Stransferases, esterases, and DDT-dehydrochlorinase were assayed. Effects of various insecticides on microsomal enzyme activities were as follows: Aldrin epoxidase activity was inhibited by malathione and permethrin treatment. N-demethylase activity was induced by diazinon and EPN treatment and O-demethlase activity was induced by diazinon treatment. Of the glutathione S-transferases, aryltransferase(DCNB conjugation) activity was significantly induced by diazinon, and moderately induced by permethrin. Of the esterases, ${\alpha}-NA$ esterase activity was moderately inhibited by malatjione and permethrin. Acetylcholinesterase activity was not affected by the sublethal exposure of honeybee to the insecticides. Sublethal exposure of honeybee to the insecticides had no effect on DDT-dehydrochlorinase activity, except carbaryl and permethrin were significantly induced.

• Journal of KIISE:Computing Practices and Letters
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• v.9 no.1
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• pp.13-24
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• 2003

MPEG-4 is a standard for multimedia applications and provides a set of technologies to satisfy the needs of authors, service providers and end users alike. In this paper, we suggest a Real-time Multiple Circular Buffer (M4RM Buffer) model, which is suitable for streaming these MPEG-4 contents efficiently. M4RM buffer generates each structure of the buffer, which matches well with each object composing an MPEG-4 content, according to the transferred information, and manipulates multiple read/write operations only by its reference. It divides the decoder buffer and the composition buffer, which are described in the standard, by the unit of frame allocated to minimize the range of access. This buffer unit of a frame is allocated according to the object description. Also, it processes the objects synchronization within the buffer and provides APIs for an efficient buffer management to process the real-time user events. Based on the performance evaluation, we show that M4RM buffer model decreases the waiting time in a buffer frame, and so allows the real-time streaming of an MPEG-4 content using the smaller size of the memory block than IM1-2D and Window Media Player.

• The Korean Journal of Pesticide Science
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• v.12 no.2
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• pp.192-196
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• 2008

This study examined the acaricidal activity of a new compound, K16776 against honeybee mite, Varroa destructor which is ecto-parasite of Apis mellifera. Acaricidal activity was performed using six acaricides, two Chinese commercial acaricides and one newly synthesized K16776 against V. destructor in the small container and in the bee hive. K16776 and amitraz exhibited 100% acaricidal activity against V. destructor without insecticidal toxicity to A. mellifera in the small container. The other acaricide was not activity. Applied to the bee hives, K16776 showed acaricidal activity as 98.7% ($250{\times}$) and 88.6% ($500{\times}$) and amitraz showed as 100% ($500{\times}$) and 90% ($1,000{\times}$), respectively. Made in China, Cao Suan Sha Man Pian and Wangs showed acaricidal activity as 56.9% and 66.7%, respectively. The result indicates that K16776 can be potentially useful control agent against honeybee mite, V. destructor.

• Journal of KIISE:Computing Practices and Letters
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• v.16 no.9
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• pp.918-926
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• 2010

A widget is a small application running by the users' favorite services, so they are provided with web contents without explicitly visiting the web site. Although widgets can be easily implemented with Open APIs, only a few web sites provide them because of refactoring the structures of web resource to supply Open APIs to the widget developers. This paper presents an Open API Proxy Server System for widget services. The system consists of two components: an Open API Source Code Generator and an Open API Proxy Server. The Open API Source Code Generator provides a Graphical User Interface (GUI) for users to generate the Open APIs of user's choice and sends the Open API source code generation request to the Open API Proxy Server. The Open API Proxy Server using the HTML Table Processing Library receives the HTML web page from web site and extracts useful information from the target HTML table. The proxy server converts the extracted data into the corresponding XML document which becomes available through the Open API. We verify the operation of the proposed system through experiments with the HTML tables in the example web sites.

• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.41-47
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• 2012

This study was examined the characteristics on the pollinating activities of Apis mellifera, Bombus ignitus and B. terrestris as pollinators on the watermelon flowers(Citrullus vulgaris Schrad), and the effects of the pollination by pollinators compared to the artificial pollination in the watermelon green houses in Nonsan City on summer season in 2005. As a result, B. ignitus and B. terrestris could not be used for summer season, because the bumblebees were not pollinated effectively indeed even two days after hives were released at watermelon green houses during the summer cultivating period. It could not be controlled a poor watermelon house conditions; high temperature and low humidity on summer season which were the bad environment for pollinating activities of bumblebees. But this study was showed that A. mellifera could be used comparatively good pollinator instead of artificial pollination in spite of the bad environment for bee within the watermelon green houses on summer season.

• Journal of Bio-Environment Control
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• v.30 no.3
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• pp.206-211
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• 2021

Hygienic behavior of Honey bees, Apis mellifera, was evaluated by uncapping and removing ability of dead broods from the nest. Hygienic behavior is originated from quantitative traits, which are expected to express key roles in colony defense against mite parasites and bacterial and fungal diseases. It is regarded as one of important characteristics of honey bee's resistance to parasites and pathogens. In this study, five inbreed and two hybrid lines of A. mellifera, the former five inbreed lines, which have been reared for over eight years at the National Academy of Agricultural Science in Korea, and the latter two hybrid lines, which have been bred by crossing between the inbreed lines, were investigated on their hygienic behavior by a pin-killed brood assay at 12hrs and 24hrs after treatment. The results indicated that after 12hrs one inbred line was proved to be hygienic (removal rate of dead brood >90%), three inbred and two hybrid lines showed intermediate behavior, and one inbred line belonged to non-hygienic (removal rate of dead brood <70%). However, after 24hrs, only one line was considered to be intermediate as removal rate was below 90%, thus all except this line had shown hygienic behavior.

• Journal of Apiculture
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• v.32 no.3
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• pp.261-268
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• 2017

We evaluated the nutritional composition including proximate, amino acid, vitamin, minerals, and the antioxidant activity of buckwheat (Fagopyrum esculentum) pollen grains collected by Apis mellifera bees, to be used as a species of forage plant with Quercus acutissima (acorn) and Actinidia arguta pollen grains. The content of crude protein and fat were found 14.43% and 5.67%, respectively. Eighteen amino acids from buckwheat pollen were found, including 8 essential amino acids. The predominant amino acids were glutamic acid, aspartic acid and lysine, accounting for about 42.7% of total free amino acids. The concentration of vitamin C was the highest value of 13.7 mg/100g, followed by $B_3$ (niacin) and $B_1$ among the detected vitamins. The predominant minerals were potassium (1197.95mg/100g), followed by phosphorus (962.77mg/100g) and magnesium (535.42mg/100g), whereas copper, zinc and manganese were detected as minor elements. Antioxidant activity and total phenolic content accounted for 8.1% at $500{\mu}g/ml$ extract and $2.25{\mu}g/mg$, respectively.