Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of Hygienic Ability for the Selection of Disease Resistant Honey Bee (Apis mellifera) Lines

질병저항성 꿀벌 계통 선발을 위한 청소능력 특성 평가

  • Kim, Hye-Kyung (Department of Industrial Entomology, Korea National College of Agriculture and Fisheries) ;
  • Park, Chang Gyu (Department of Industrial Entomology, Korea National College of Agriculture and Fisheries) ;
  • Han, Gug-In (Industrial Entomology Research Institute)
  • 김혜경 (한국농수산대학 산업곤충학과) ;
  • 박창규 (한국농수산대학 산업곤충학과) ;
  • 한국인 (충청남도농업기술원 산업곤충연구소)
  • Received : 2021.06.25
  • Accepted : 2021.07.17
  • Published : 2021.07.31
Download PDF
⟨ Previous Next ⟩

Abstract

Hygienic behavior of Honey bees, Apis mellifera, was evaluated by uncapping and removing ability of dead broods from the nest. Hygienic behavior is originated from quantitative traits, which are expected to express key roles in colony defense against mite parasites and bacterial and fungal diseases. It is regarded as one of important characteristics of honey bee's resistance to parasites and pathogens. In this study, five inbreed and two hybrid lines of A. mellifera, the former five inbreed lines, which have been reared for over eight years at the National Academy of Agricultural Science in Korea, and the latter two hybrid lines, which have been bred by crossing between the inbreed lines, were investigated on their hygienic behavior by a pin-killed brood assay at 12hrs and 24hrs after treatment. The results indicated that after 12hrs one inbred line was proved to be hygienic (removal rate of dead brood >90%), three inbred and two hybrid lines showed intermediate behavior, and one inbred line belonged to non-hygienic (removal rate of dead brood <70%). However, after 24hrs, only one line was considered to be intermediate as removal rate was below 90%, thus all except this line had shown hygienic behavior.

화분매개곤충으로 중요한 산업적 가치를 지닌 꿀벌은 전 세계적으로 병해충에 의한 개체수 감소 현상이 심각한 문제로 제기 되고 있다. 본 논문에서는 이를 타개하기 위하여 질병저항성 꿀벌 계통을 선발하고자 하였으며, 이를 위해 꿀벌 육성 계통별 청소력(hygienic behavior) 평가를 수행하였다. 이는 질병 저항성 계통 선발에 관한 연구로본 연구에서는 5종의 꿀벌 계통과 2종의 교배종 계통에 대한 청소력 평가를 Pin killed brood assay 법에 의해 수행하였다. 그 결과 처리 24 시간 후 죽은 번데기를 제거하거나 개봉하는 능력이 60% 이상으로 청소능력이 낮은 계통(non-hygienic)은 확인되지 않았으며, 반대로 죽은 번데기를 제거하거나 개봉하는 능력이 90%이상인 hygienic 계통은 C, D, E, F, ♀CX♂D 그리고 ♀FX♂D 계통인 것으로 드러났으며, 그 외A 계통은 보통(intermediate)의 청소능력을 가진 것으로 확인 되었다. 이를 통해 본 연구에서는 육성 계통 대부분이 높은 질병저항성 특성을 지니는 것으로 평가되고 있으며, 이러한 결과는 지속적인 질병저항성 꿀벌 계통 선발에 활용할 수 있을 것으로 기대되고 있다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 공동연구사업사업 PJ01576304과 PJ01504206 과제의 지원에 의해 수행된 결과입니다.

References

  1. Boecking O., and M. Spivak 1999, Behavioral defenses of honey bees against Varroa jacobsoni Oud. Apidologie 30:141-158. doi:10.1051/apido:19990205
  2. Boutin S., M. Alburaki, P.L. Mercier, P. Giovenazzo, and N. Derome 2015, Differential gene expression between hygienic and non-hygienic honeybee (Apis mellifera L.) hives. BMC Genomics 16:500. doi:10.1186/s12864-015-1714-y
  3. Dahle B. 2010, The role of Varroa destructor for honey bee colony losses in Norway. Journal of Apicultural Research 49:124-125. doi:10.3896/IBRA.1.49.1.26
  4. Danka R.G., J.W. Harris, and J.D. Villa 2011, Expression of Varroa sensitive hygiene (VSH) in commercial VSH honey bees (Hymenoptera: Apidae). J Econ Entomol 104:745-749. doi:10.1603/EC10401
  5. Genersch E. 2010, Honey bee pathology: current threats to honey bees and beekeeping. Applied Microbiology and Biotechnology 87:87-97. doi:10.1007/s00253-010-2573-8
  6. Gerdts J., R.L. Dewar, M.S. Finstorm, T. Edwards, and M. Angove 2018, Hygienic behavior selection via freeze-killed honey bee brood no associated with chalkbrood resistance in eastern Australia. Plos One 13:e0203969. doi:10.1371/journal.pone.0203969
  7. Gramacho K., and M. Spivak 2003, Differences in olfactory sensitivity and behavioral responses among honey bees bred for hygienic behavior. Behav Ecol Sociobiol 54:472-479. doi:10.1007/s00265-003-0643-y
  8. Higes M., A. Meana, C. Bartolome, C. Botias, and R. Martin-Hernandez 2013, Nosema ceranae (Microsporidia), a controversial 21st century honey bee pathogen. Environmental Microbiology Reports 5:17-29. doi:10.1111/1758-2229.12024
  9. Invernizzi C., F. Rivas, and L. Betticci 2011, Resistance to chalkbrood disease in Apis melifera L. (Hymenoptera: Apidae) colonies with different hygienic behavior. Neotropical Entomol 40:28-34. https://doi.org/10.1590/S1519-566X2011000100004
  10. Jensen A.B., K.A. Palmer, J.J. Boomsma, and B.V. Pedersen 2005, Varying degrees of Apis mellifera ligustica introgression in protected populations of the black honeybee, Apis mellifera mellifera, in northwest Europe. Mol Ecol 14:93-106. doi: 10.1111/j.1365-294X.2004.02399.x
  11. Jeremy T.K., P. Alana, G. Paul, P. Laurence, G.P. Simon, M.R. Stuart, R. Pierre, O.S. liver, R.C. Sheila, L.R. Leif, L.W. David, F.G. Lawrence, S.S. Derek, and P. Alberto 2015, Climate change impacts on bumblebees converge across continents. Science 349:177-180. doi:10.1126/science.aaa7031
  12. Jung C. 2008, Economic Value of Honeybee Pollination on Major Fruit and Vegetable Crops in Korea. J Apiculture 23:147-152.
  13. Kim H.K., M.Y. Lee, M.L. Lee, Y.S. Choi, and K.H. Byeon 2015, Hygienic behavior test of six inbred lines in Apis mellifera through Freeze-killed brood method. J Apiculture 30:187-190. https://doi.org/10.17519/apiculture.2015.09.30.3.187
  14. Kim Y., J. Kim, Y. Oh, S. Lee, S. Song, E. Joung, S. Lee, S. Lee, and B. Moon 2016, Prevalence of honey bee (Apis mellifera) disease in Daejeon. Korean J Vet Serv 39:253-258. https://doi.org/10.7853/KJVS.2016.39.4.253
  15. Lapidge K.L., B.P. Oldroyd, and M. Spivak 2002, Seven suggestive quantitative trait loci influence hygienic behavior of honey bees. Naturwissenschaften 89:565-568. doi:10.1007/s00114-002-0371-6
  16. Oxley P.R., and B.P. Oldroyd 2010, The genetic architecture of honeybee breeding. Advances in Insect Physiology 39: 83-118. doi:10.1111/j.1365-294X.2004.02399.x|
  17. Palacio M.A., E.M. Rodriguez, L. Goncalves, E. Bedascarrasbure, and M. Spivak 2010, Hygienic behaviors of honey bees in response to brood experimentally pin killed or infected with Ascosphaera apis. Apidologie 41:602-612. doi:10.1051/apido/2010022
  18. Rothenbuhler W.C. 1964, Behavior genetics of nest cleaning in honey bees. IV. Responses of F1 and backcross generations to disease-killed brood. Am Zool 4:111-123. https://doi.org/10.1093/icb/4.2.111
  19. Spivak M. 1996, Honey bee hygienic behavior and defense against Varroa jacobsoni. Apidologie 27:245-260. doi:10.1051/apido:19960407
  20. Spivak M., and D.L. Downey 1998, Field assays for hygienic behavior in disease resistance in honey bees (Hymenoptera; Apidae). J Eco Entomol 91:64-70. https://doi.org/10.1093/jee/91.1.64
  21. Spivak M., and G.S. Reuter 2001, Resistance to American foulbrood disease by honey bee colonies Apis mellifera bred for hygienic behavior. Apidologie 32:555-565. doi:10.1051/apido:2001103
  22. Tsuruda J.M., J.W. Harris, L. Bourgeois, R.G. Danka, and G.J. Hunt 2012, High-resolution linkage analyses to identify genes that influence Varroa sensitive hygiene behavior in honey bees. Plos One 7:e48276. doi:10.1371/journal.pone.0048276
  23. Wilson-Rich N., S.T. Dres, and P.T. Starks 2008, The ontogeny of immunity: development of innate immune strength in the honey bee (Apis mellifera). J Insect Physiol 54:1392-9. doi: 10.1016/j.jinsphys.2008.07.016
  24. Han J.H. 2015, A Study on Management of Apiary and Main Factors for Developing the Beekeeping Industry in Korea. Journal of Apiculture 30:127-133. https://doi.org/10.17519/apiculture.2015.06.30.2.127