• The Plant Pathology Journal
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• v.19 no.3
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• pp.159-161
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• 2003

The coat protein (CP) gene of Apple mosaic virus (ApMV), a member of the genus Ilarvirus, was selected for the design of virus-specific primers for amplification and molecular detection of the virus in cultivated apple. A combined assay of reverse transcription and polymerase chain reaction (RT-PCR) was performed with a single pair of ApMV-specific primers and crude nucleic acid extracts from virus-infected apple for rapid detection of the virus. The PCR product was verified by restriction mapping analysis and by sequence determination. The lowest concentration of template viral RNA required for detection was 100 fg. This indicates that the RT-PCR for detection of the virus is a 10$^3$times more sensitive, reproducible and time-saving method than the enzyme-linked immunosorbent assay. The specificity of the primers was verified using other unrelated viral RNAs. No PCR product was observed when Cucumber mosaic virus (Cucumovirus) or a crude extract of healthy apple was used as a template in RT-PCR with the same primers. The PCR product (669 bp) of the CP gene of the virus was cloned into the plasmid vector and result-ant recombinant (pAPCP1) was selected for molecule of apple transformation to breed virus-resistant transgenic apple plants as the next step. This method can be useful for early stage screening of in vitro plantlet and genetic resources of resistant cultivar of apple plants.

• Journal of Internet Computing and Services
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• v.9 no.2
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• pp.43-50
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• 2008

In IEEE 802.11b wireless LAN, stations keep synchronization by receiving beacons being periodically sent by the AP (access point) when they run in low power mode. Beacon transmission is delayed if wireless channel is busy at a scheduled TBTT (target beacon transmission time), and stations must be awake until they receive the delayed beacon. In this paper, we propose a scheme that stations get their transmission delayed if they have little time for successful data transmission by the next TBTT. Beacon transmission without delay is guaranteed at every TBTT with this method so that the awake time of low power stations to receive beacon can be reduced. The proposed method is simulated and its characteristics ore described with the analysis of the results. The measured results in terms of beacon delay show some enhancement in energy consumption.

• Journal of the Korean earth science society
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• v.39 no.5
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• pp.473-482
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• 2018

During the period of 2002 to 2017, the Gravity Recovery And Climate Experiment (GRACE) had observed time-varying gravity changes with unprecedented accuracy. The GRACE science data centers provide the monthly gravity solutions after removing the sub-monthly mass fluctuation using geophysical models. However, model misfit makes the solutions to be contaminated by aliasing errors, which exhibits peculiar north-south stripes. Two conventional filters are used to reduce the errors, but signals with similar spatial patterns to the errors are also removed during the filtering procedure. This would be particularly problematic for estimating the ice mass changes in Western Antarctic Ice Sheet (WAIS) and Antarctic Peninsula (AP) due to their similar spatial pattern to the elongated north-south direction. In this study, we introduce an alternative filter to remove aliasing errors using the Empirical Orthogonal Functions (EOF) analysis. EOF can decompose data into different modes, and thus is useful to separate signals from noise. Therefore, the aliasing errors are effectively suppressed through EOF method. In particular, the month-to-month mass changes in WAIS and AP, which have been significantly contaminated by aliasing errors, can be recovered using EOF method.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.10a
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• pp.47-49
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• 2022

IPS is an indoor positioning system that replaces GPS, which is less accurate due to walls and roofs inside buildings. Radio signals such as Wi-Fi are used instead of satellite signals, and complex methods are being used to increase accuracy. In this paper, we present a more efficient positioning method than before by applying high-level signals to high-accuracy fingerprint techniques. A radio map is configured with high-level signals collected by utilizing Wi-Fi APs, which are more than in the past. The suitability of use was confirmed by analyzing the pattern represented by the configured radio map for each location.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.20 no.9
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• pp.1704-1709
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• 2016

In this paper, we propose efficient uplink data transmission method in ultra dense wireless networks as in intra-aircraft, where large-scale APs and wireless sensors are deployed. In the ultra dense wireless networks, a performance degradation is inevitable due to the inter-AP interference. However, the performance degradation can be avoided if a scheduling algorithm can estimate the amount of interference caused by each wireless sensor and reflects it. SGIR (Signal-to-Generating Interference Ratio) based scheduling algorithms is a typical example. Unfortunately, the scheduling algorithms based on the interference caused by wireless sensors necessarily yield large scale exchange of information through backhaul which connects APs. Therefore, we, in this paper, propose a novel scheme which can dramatically reduce the amount of information which are exchanged through backhaul connection. Monte-Carlo simulation results show that the proposed scheme can reduce the amount of backhaul traffic by 27% without loss of data transmission rate.

• International Journal of Highway Engineering
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• v.7 no.1 s.23
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• pp.49-61
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• 2005

The dynamic modulus of hot mix asphalt can be determined according to the different combinations of testing temperature and loading frequency. The superposition rule is adapted to get the master curve of dynamic modulus for each hot mix asphalt. There are couple of different methods to get the shift factor which is a key for making the master curve. In this paper, Arrehnius, 2002 AASHTO, and experimental method was employed to get the master curve. Evaluation of dynamic modulus for 25mm base course of hot mix asphalt with granite aggregate and two asphalt binders(AP-3 and AP-5) was carried out. Superpave Level 1 Mix Design with gyratory compactor was adopted to determine the optimum asphalt binder content(OAC) and the measured ranges of OAC were between 4.1% and 4.4%. UTM was used for laboratory test. The dynamic modulus and phase angle were determined by testing on UTM, with 5 different testing temperature(-10, 5, 20, 40, & $55^{\circ}C$) and 5 different loading frequencies(0.05, 0.1, 1, 10, 25 Hz). Using the measured dynamic modulus and phase angle, the input parameters of Sigmoidal function equation to represent the master curve were determined and these will be adopted in FEM analysis for asphalt pavements. The shift factor and activation energy for determination of master curve were calculated.

• Applied Biological Chemistry
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• v.39 no.2
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• pp.147-152
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• 1996

To screen inhibitors on complement system from natural resources, micro-screening method was established by using hemolytic complement assay. Complement fixation reaction was carried out in the microplate system. For standard hemolysis (50% hemolysis) of the classical pathway (CP), hemolysin and complement serum were diluted to $1/75{\sim}1/100\;and\;1/80{\sim}1/120$, respectively, when sheep erythrocytes were $5.0{\times}10^8\;cells/ml$. In case of the alternative pathway (AP), complement serum was diluted to 1/5 and EGTA and $Mg^{2+}$ were added 4 mM, $4{\sim}8\;mM$, respectively, when rabbit erythrocytes were $4.0{\times}10^8\;cells/ml$. Dimethyl sulfoxide was used for the assay of non-aquous soluble compounds or extracts and its final concentration was not more than 1%. Three phenylpropanoids showed anticomplementary activities in proportion to the concentration for both pathways and rosmarinic acid exihibited the highest inhibitory activities: $5.4{\pm}3.6%(0.063\;mM){\sim}95.8{\pm}0.2%(0.5\;mM)\;and\;35.1{\pm}0.9%(0.063\;mM){\sim}95.6{\pm}1.1%(1\;mM)$ on the CP and the AP, respectively.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.10a
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• pp.436-438
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• 2015

In this paper, a P2P Standard Group Formation (P2P-SGF) method in Wi-Fi Direct is presented to provide direct communication services between Internet-of-Things (IoT) gateways. Generally, network traffic congestion and speed reduction problems are caused when transmitting mass data as well as measured data from many sensors between IoT gateways via an Access Point (AP). For solving these problems, it is required to implement Wi-Fi Direct efficiently transmitting mass data between IoT gateways without passing through the AP. The implemented P2P-SGF method basically performs Discovery, GO (Group Owner) Negotiation, WPS (Wi-Fi Protected Setup) Provisioning, and Address Configuration. Especially, it supports the DLNA (Digital Living Network Alliance) service function for sharing mass data between IoT gateways. Some experimental results verify that it can successfully share large video files.

• Journal of Broadcast Engineering
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• v.27 no.3
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• pp.391-401
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• 2022

As deep learning-based object detection and recognition research have been developed recently, the scope of application to industry and real life is expanding. But deep learning-based systems in the construction system are still much less studied. Calculating materials in the construction system is still manual, so it is a reality that transactions of wrong volumn calculation are generated due to a lot of time required and difficulty in accurate accumulation. A fast and accurate automatic drawing recognition system is required to solve this problem. Therefore, we propose an AI-based automatic drawing recognition accumulation system that detects and recognizes steel materials in construction drawings. To accurately detect steel materials in construction drawings, we propose data augmentation techniques and spatial attention modules for improving small object detection performance based on YOLOv4. The detected steel material area is recognized by text, and the number of steel materials is integrated based on the predicted characters. Experimental results show that the proposed method increases the accuracy and precision by 1.8% and 16%, respectively, compared with the conventional YOLOv4. As for the proposed method, Precision performance was 0.938. The recall was 1. Average Precision AP_0.5 was 99.4% and AP_0.5:0.95 was 67%. Accuracy for character recognition obtained 99.9.% by configuring and learning a suitable dataset that contains fonts used in construction drawings compared to the 75.6% using the existing dataset. The average time required per image was 0.013 seconds in the detection, 0.65 seconds in character recognition, and 0.16 seconds in the accumulation, resulting in 0.84 seconds.

• Journal of The Korean Association For Science Education
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• v.42 no.2
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• pp.289-309
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• 2022

In recent years, astronomy has been snowballing: including Higgs particle discovery, black hole imaging, extraterrestrial exploration, and deep space observation. Students are also largely interested in astronomy. The purpose of this study is to discover what needs to be improved in the current astronomy curriculum in light of recent scientists' researches and discoveries. We collected keywords from all papers published from 2011 to 2020 in four selected journals-ApJ, ApJL, A&A, and MNRAS- by R package to examine research trends. The curriculum contents were extracted by synthesizing the in-service teachers' coding results in the 2015 revised curriculum document of six subjects (Science, Integrated Science, Earth Science I, Earth Science II, Physics II, Convergence Science). The research results are as follows: first, keywords that appear steadily in astronomy are 'galaxies: formation, galaxy: active, star: formation, accretion, method: numerical.' Second, astronomy curriculum includes all areas except the 'High Energy Astrophysical Phenomena' area within the common science curriculum learned by all students. Third, it is necessary to review the placement of content elements by subject and grade and to consider introducing new concepts based on astronomy research keywords. This is an exploratory study to compare curriculum and the field of scientific research that forms the basis of the subject. We expect to provide implications for a future revision of the astronomy curriculum as a primary ground investigation.