• Genes and Genomics
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• v.40 no.12
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• pp.1287-1300
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• 2018

Hydrogen sulfide ($H_2S$), a small bioactive gas, has been proved functioning in plant growth and development as well as alleviation of abiotic stresses, which including promoting seed germination, accelerating embryonic root growth, regulating flower senescence, inducing stomatal closure, and defending drought, heat, heavy metals and osmotic stresses etc. However, the molecular functioning mechanism of $H_2S$ was still unclear. The primary objective of this research was to analyze the transcriptional differences and functional genes involved in the $H_2S$ responses. In details, 4-week-old plantlets in tissue culture of grapevine (Vitis vinifera L.) cultivar 'Zuoyouhong' were sprayed with 0.1 mM NaHS for 12 h, and then transcriptome sequencing and qRT-PCR analysis were used to study the transcriptional differences and functional genes involved in the $H_2S$ responses. Our results indicated that 650 genes were differentially expressed after $H_2S$ treatment, in which 224 genes were up-regulated and 426 genes were down-regulated. The GO enrichment analysis and KEGG enrichment analysis results indicated that the up-regulated genes after $H_2S$ treatment focused on carbon metabolism, biosynthesis of amino acids, and glycolysis/gluconeogenesis, and the down-regulated genes were mainly in metabolic pathways, biosynthesis of secondary metabolites, and plant hormone signal transduction. Analyzing the transcription factor coding genes in details, it was indicated that 10 AP2/EREBPs, 5 NACs, 3 WRKYs, 3 MYBs, and 2 bHLHs etc. transcription factor coding genes were up-regulated, while 4 MYBs, 3 OFPs, 3 bHLHs, 2 AP2/EREBPs, 2 HBs etc. transcription factor coding genes were down-regulated. Taken together, $H_2S$ increased the productions in secondary metabolites and a variety of defensive compounds to improve plant development and abiotic resistance, and extend fruits postharvest shelf life by regulating the expression of AP2/EREBPs, WRKYs, MYBs, CABs, GRIP22, FERRITINs, TPSs, UGTs, and GHs etc.

• Journal of information and communication convergence engineering
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• v.8 no.4
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• pp.399-404
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• 2010

We propose a seamless IPTV transport technology of mobile device. This technology uses multiple interfaces of wireless communication on mobile device. This proposal for transportation of mobile IPTV contents is more mobile and adaptable than other wireless technologies that are currently being used. Algorithm of proposed technology is as follow. When the signal strength of the connected wireless network interface of mobile device is getting lower than specified level, another wireless network interface is connected to continue downloading the IPTV contents in advance. Another connection is maintained until the signal strength of the first connected interface is stable or the interface is connected to another base station (or AP) that have good signal strength. For more seamless services, we consider classifying the packets of mobile IPTV and using dynamic content quality select techniques. Dynamic content quality selection is based on notifying transfer rate to the content delivery network (CDN) server. The proposed technology is expected to use efficiently with both mobile IPTV and the transportation of mobile P2P/P4P.

• The KIPS Transactions:PartD
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• v.19D no.3
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• pp.237-246
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• 2012

Although the development of Global Positioning System (GPS) are more and more mature, its accuracy is just acceptable for outdoor positioning, not positioning for the indoor of building and the underpass. For the positioning application area for the indoor of building and the underpass, GPS even cannot achieve that accuracy because of the construction materials while the requirement for accurate positioning in the indoor of building and the underpass, because a space, a person is necessary, may be very small space with several square meters in the indoor of building and the underpass. The Received Signal Strength (RSS) based localization is becoming a good choice especially for the indoor of building and the underpass scenarios where the WiFi signals of IEEE 802.11, Wireless LAN, are available in almost every indoor of building and the underpass. The fundamental requirement of such localization system is to estimate location from Access Point (AP) to mobile device using RSS at a specific location. The Multi-path fading effects in this process make RSS to fluctuate unpredictably, causing uncertainty in localization. To deal with this problem, the combination for the method of Neural Networks and Push-Pull Estimation is applied so that the carried along the devices can learn and make the decision of position using mobile device where it is in the indoor of building and the underpass.

• Journal of Life Science
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• v.33 no.2
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• pp.176-182
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• 2023

The stems of Dendrobium moniliforme are used in traditional Oriental medicine as a Yin tonic to nourish the stomach, promote the production of body fluid, and reduce fever. This study investigated the effects of the aqueous extract of D. moniliforme stems (DME) on mast cell degranulation and the expression of tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), and histamine-synthesizing enzyme histidine decarboxylase (HDC). We used rat mast cell line RBL-2H3 cells and stimulated them with PMA plus calcium ionophore (PMACI). Pretreatment with DME significantly inhibited PMACI-induced β-hexosaminidase release and the expression of TNF-α, IL-4, and HDC. Furthermore, DME suppressed PMACI-induced nuclear translocation of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and activator protein 1 (AP-1). In addition, HDC expression was inhibited by SP600125 (JNK inhibitor), PD98059 (ERK inhibitor), and SB203580 (p38 kinase inhibitor). Finally, the phosphorylation of p38 kinase, extracellular signal-regulated kinase 1/2 (ERK1/2), and c-Jun N-terminal kinase (JNK) was inhibited by pretreatment with DME. These results suggest that DME has inhibitory effects against degranulation, cytokine (TNF-α and IL-4) and HDC expression, and that HDC expression is mediated by MAPK signaling. These findings suggest that DME may have therapeutic potential in the treatment of hypersensitive and inflammatory diseases.

• BMB Reports
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• v.53 no.4
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• pp.218-222
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• 2020

Excessive and hyperactive osteoclast activity causes bone diseases such as osteoporosis and periodontitis. Thus, the regulation of osteoclast differentiation has clinical implications. We recently reported that dehydrocostus lactone (DL) inhibits osteoclast differentiation by regulating a nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), but the underlying mechanism remains to be elucidated. Here we demonstrated that DL inhibits NFATc1 by regulating nuclear factor-κB (NF-κB), activator protein-1 (AP-1), and nuclear factor-erythroid 2-related factor 2 (Nrf2). DL attenuated IκBα phosphorylation and p65 nuclear translocation as well as decreased the expression of NF-κB target genes and c-Fos. It also inhibited c-Jun N-terminal kinase (JNK) but not p38 or extracellular signal-regulated kinase. The reporter assay revealed that DL inhibits NF-κB and AP-1 activation. In addition, DL reduced reactive oxygen species either by scavenging them or by activating Nrf2. The DL inhibition of NFATc1 expression and osteoclast differentiation was less effective in Nrf2-deficient cells. Collectively, these results suggest that DL regulates NFATc1 by inhibiting NF-κB and AP-1 via down-regulation of IκB kinase and JNK as well as by activating Nrf2, and thereby attenuates osteoclast differentiation.

• Journal of Life Science
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• v.33 no.1
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• pp.34-42
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• 2023

In a signal transduction network, from the perception of stress signals to stress-responsive gene ex- pression, binding of various transcription factors to cis-acting elements in stress-responsive promoters coordinate the adaptation of plants to abiotic stresses. Among the AP2/ERF transcription factor family genes, group VII ERF genes, such as RAP2.12, RAP2.2, RAP2.3, AtERF73/HRE1, and AtERF71/ HRE2, are known to be involved in the response to hypoxia stress in Arabidopsis. In this study, we dissected the HRE1 promoter to identify hypoxia-responsive region(s). The 1,000 bp upstream promoter region of HRE1 showed increased promoter activity in Arabidopsis protoplasts and transgenic plants under hypoxia conditions. Analysis of the promoter deletion series of HRE1, including 1,000 bp, 800 bp, 600 bp, 400 bp, 200 bp, 100 bp, and 50 bp upstream promoter regions, using firefly luciferase and GUS as reporter genes indicated that the -200 to -100 region of the HRE1 promoter is responsible for the transcriptional activation of HRE1 in response to hypoxia. In addition, we identified two putative hypoxia-responsive cis-acting elements, the ERF-binding site and DOF-binding site, in the -200 to -100 region of the HRE1 promoter, suggesting that the expression of HRE1 might be regulated via the ERF transcription factor(s) and/or DOF transcription factor(s). Collectively, our results suggest that HRE1 contains hypoxia-responsive cis-acting elements in the -200 to -100 region of its promoter.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.671-674
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• 2005

Atmospheric correction over the ocean part is more important than that over the land because the signal from the ocean is very small about one tenth of that reflected from land. In this study, the Spectral Shape Matching Method (SSMM) developed by Ahn and Shanmugam (2004) is evaluated using Landsat imagery acquired over the highly turbid Saemangeum Coastal Area. The result of SSMM is compared with COST model developed by Chavez (1991 and 1997). In principle, SSMM is simple and easy to implement on any satellite imagery, relying on both field and image properties. To assess the potential use of these methods, several field campaigns were conducted in the Saemangeum coastal area corresponding with Landsat-7 satellite's overpass on 29 May 2005. In-situ data collected from the coastal waters of Saemangeum using optical instruments (ASD field spectroradiometer) consists of ChI, Ap, SS, aooM, F(d). In order to perform SSMM, we use the in-situ water-leaving radiance spectra from clear oceanic waters to estimate the the path radiance from total signal recorded at the top of the atmosphere (TOA), due to the reason that the shape of clear water-leaving radiance spectra is nearly stable than turbid water-leaving radiance spectra. The retrieved water-leaving radiance after subtraction of path signal from TOA signal in this way is compared with that estimated by COST model. The result shows that SSMM enabled retrieval of water-leaving radiance spectra that are consistent with in-situ data obtained from Saemangeum coastal waters. The COST model yielded significantly high errors in these areas.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.10
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• pp.2177-2184
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• 2012

A different and effective method for indoor positioning system is needed and increased it's importance compare to the outdoor GPS based method. The FingerPrint positioning method is known as a superior method in indoor positioning system that maintains signal strength patterns for RPs(Reference Points) in database and compare the DB with the measured real-time signals on the mobile device. FingerPrint positioning method is necessary to establish an accurate database, but errors can occur by several factors. In this paper, we analyze the signal patterns of each terminal in accordance with connection state of access point and trace that the error in accordance with connection state of access point can be an important error in FingerPrint DB configuration through an experimental case study.

• Natural Product Sciences
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• v.15 no.3
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• pp.156-161
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• 2009

Rubus coreanus, commonly known as 'red raspberry' is used as a traditional oriental medicine in Korea for the management of diseases such as impotence, spermatorrhea and athsma, and for allergies, in combination with other herbal preparations, in many centuries. We undertook a comparison of the hepatoprotective effect of ethanol extracts of the unripe (UREx) and ripe (RREx) R. coreanus extract against acetaminophen (AAP) induced hepatotoxicity in rats. UREx reduced the elevated alanine aminotransferase (ALP), aspartate aminotransferase (AST), total bilirubin (TB), alkaline phosphatase (AP), lipid peroxide and nitric oxide content which had been increased by AAP administration. UREx also increased the cellular glutathione (GSH) content and induced the glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px) content which had been decreased by AAP. RREx did not exhibit strong hepatoprotective effect or antioxidant activity under the same conditions. The experimental results show that the degree of the ripening of R. coreanus affects the hepatoprotective activity in the AAP-intoxicated rats. These findings of a protective mechanism are supportive evidence for the utility of unripened R. coreanus in traditional medicine for liver ailments.

• Journal of Chest Surgery
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• v.29 no.10
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• pp.1170-1172
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• 1996

Chest wall hamartoma Is a very rare disease. The female infant was suffered from frequent upper respiratory infection. The chest AP revealed destruction of the ribs and widening of the intercostal space Chest CT demonstrated well-defined solid and cystic extrapleural mass. Chest M Rl revealed high signal and low signal intensities In the mass. In December, 1995, she underwent excision of the mass with partial resection of the ribs and ch st wall reconstruction with thick Cortex patch. The chest wall hamartoma was confirmed with histopathological examination. The postop course was smooth and uneventful.