• Science of Emotion and Sensibility
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• v.22 no.4
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• pp.57-64
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• 2019

Anger is the most common emotional trigger causing relapses in individuals with alcohol use disorders (AUDs). The present study intended to investigate the autonomic nervous system (ANS) responses induced by anger in individuals with AUDs. The participants in this study included twelve individuals with AUDs and 14 non-frequent drinkers. Anger was induced in the participants via a 120-second film clip. Before the presentation of this audio-visual stimulus, the ANS responses of the participants were measured for 60 seconds to ascertain their resting state. Subsequently, the participants' ANS responses were measured again for 120 seconds when they were in an emotional state during the presentation of the clip. After the ANS measurements were taken, participants were asked to rate the type of emotion they had experienced as they viewed the film and to report its intensity. The results indicated that the levels of anger experienced by the AUD group were not significantly different from the emotion registered by the control group. However, the ANS responses induced in AUD participants when they were in an emotional state showed blunted skin conductance levels (SCL) and skin conductance responses (SCR) compared to the control group participants. Individuals with AUDs evinced lower emotional arousal than the participants of the control group. These results can help clinicians understand the psychological and physiological responses of individuals with AUDs to anger in order to design effective interventions that would reduce chances of anger and relapse.

• Science of Emotion and Sensibility
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• v.10 no.2
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• pp.263-271
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• 2007

A review of recent studies indicates that positive emotions help buffer stress. In particular, humor is what only human can appreciate. Humor plays an important role in many facets of human life including psychological, social, and somatic functioning. This study is to identify children's ANS responses by humor or joy using audio-visual film clips. 49 male and female children (12-13 years) participated in this study. The participants were briefed on the experiment was its procedure. During the experiment, electrodes were attached to participant and middle fingers of left hands. Physiological responses(EDA, SKT, PPG and ECG) were measured for 30s both in the resting state and experiment state during which emotion provoking stimulus was presented for 2 min. Also, self-report on emotions was followed for 1 min. Results from the self-report showed that both humor and joy were evoked effectively among 89.3% of children. It explains that emotion-eliciting stimuli were effective and appropriate. ANS responses in both emotion were significant in three physiological variables (i.e., SCL, NSCR, LF). There was a significant difference between humor emotion and joy emotion, and both emotions were distinguishable by a specific ANS response pattern.

• Science of Emotion and Sensibility
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• v.10 no.3
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• pp.471-480
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• 2007

Negative emotions play an important function as to human's existence. In this research, we employed the audio-visual film clips to induce negative emotions and examined the classified responses in the autonomic nervous system(ANS) due to each negative emotion.30 adults(22.6 years $old{\pm}1.24$, 15 males and 15 females) took part in this experiment. Through the preliminary experiment, 2 minutes film's stimuli were selected as the emotion-induced stimuli. During the period when participants were viewing and listening to the selected movie, EDA and ECG were examined as soon as one stimulus was displayed, participants were tested by completing the psychological appraisals of their experienced emotion due to each emotional stimulus. With regard to the result of analyzing the psychological responses, each negative emotion appropriately and effectively induced its target emotion. While concerning the result of analyzing ANS responses, each negative emotion induced its respective activation in ANS. What is more, compared with other types of negative emotional stimuli, the scaring stimulus induced higher activation of the sympathetic nervour system(SNS) as to the indexes in EDh and ECG. This research made segmentation of ANS responses to each negative emotion, which has its significance.

• The Korean Journal of Pain
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• v.35 no.3
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• pp.327-335
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• 2022

Background: The pathophysiology of fibromyalgia (FM) involves many mechanisms including central nervous system sensitization theory, autonomic nervous system (ANS) dysfunction, and recently small fiber neuropathy. While the small fiber neuropathy itself can cause ANS dysfunction and neuropathic pain (NP), it is still unknown whether ANS problems have an association with severity of disease and NP in patients with FM. The aim of this study was to evaluate ANS dysfunction in FM patients and to explore possible associations of ANS dysfunction with disease severity and NP. Methods: Twenty-nine FM patients and 20 healthy controls were included in this cross-sectional study. Participants were tested using sympathetic skin responses (SSR) and R-R interval variation analyses for sympathetic and parasympathetic ANS dysfunction, respectively. Disease severity and somatic symptoms of patients with FM were evaluated using the ACR-2010 scales and Fibromyalgia Impact Questionnaire, and NP symptoms were evaluated using the Pain Detect Questionnaire and Douleur Neuropathique questionnaire. Results: FM patients were found to have ANS dysfunction characterized by increased sympathetic response and decreased parasympathetic response. SSR amplitudes were found to be correlated with a more severe disease. Although nonsignificant, NP severity tended to be associated with a decrease in sympathetic and parasympathetic activities. Conclusions: ANS dysfunction may play a role in the pathophysiology of FM. The trend of decreased ANS functions in FM patients exhibiting NP contradicts the notion that FM is a sympathetically maintained NP and may be explained with small fiber involvement.

• Molecules and Cells
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• v.26 no.6
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• pp.536-547
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• 2008

Anthocyanidin synthase (ANS, leucoanthocyanidin oxygenase), a 2-oxoglutarate iron-dependent oxygenase, catalyzed the penultimate step in the biosynthesis of the anthocyanin class of flavonoids, from the colorless leucoanthocyanidins to the colored anthocyanidins. The full-length cDNA and genomic DNA sequences of ANS gene (designated as GbANS) were isolated from Ginkgo biloba for the first time. The full-length cDNA of GbANS contained a 1062-bp open reading frame (ORF) encoding a 354-amino-acid protein. The genomic DNA analysis showed that GbANS gene had three exons and two introns. The deduced GbANS protein showed high identities to other plant ANSs. The conserved amino acids (H-X-D) ligating ferrous iron and residues (R-X-S) participating in 2-oxoglutarate binding were found in GbANS at the similar positions like other ANSs. Southern blot analysis indicated that GbANS belonged to a multi-gene family. The expression analysis by real-time PCR showed that GbANS expressed in a tissue-specific manner in G. biloba. GbANS was also found to be up-regulated by all of the six tested abiotic stresses, UV-B, abscisic acid, sucrose, salicylic acid, cold and ethylene, consistent with the promoter region analysis of GbANS. The recombinant protein was successfully expressed in E. coli strain with pET-28a vector. The in vitro enzyme activity assay by HPLC indicated that recombinant GbANS protein could catalyze the formation the cyanidin from leucocyanidin and conversion of dihydroquercetin to quercetin, suggesting GbANS is a bifunctional enzyme within the anthocyanidin and flavonol biosynthetic pathway.

• Science of Emotion and Sensibility
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• v.10 no.3
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• pp.463-470
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• 2007

There have been many studies on patients who suffer from anxiety disorders. However, there is been not enough attention on the difference in the level of between the two populations with and without anxiety disorders. This study was performed to investigate the difference in the ANS responses induced by fear in children. Experimental procedures were as follow: All subjects were in upper grade levels in elementary school. ANX(anxiety) scales of PIC(Personality Inventory for Children) were used to measure fear anxiety. Audio-visual clips were used as stimulus to provoke fear emotion. Baseline of physiological signals, ECG, PPG, EDA, and SKT, were measured for 30 seconds before the fear stimulus. Physiological signals were then recorded for 2 minutes while fear is evoked. Psychological and physiological responses were analyzed. All the children reacted to the fear stimulus with high intensity of fear. Physiological responses showed that SKT, SCR, NSCR, HR, RSA, RESP, HF were increased, while R-R was significantly decreased, respectively, during the period of fear induction. Analysis of the level of anxiety and the physiological responses produced by the experience of fear revealed a statistically significant positive correlation in SKT, HR, and RSA. In other words, the higher the level of anxiety, the higher the levels of SKT, HR, and RSA when children experienced fear in conclusion, it is confirmed through this research that physiological responses to fear is associated with the level of anxiety each individual.

• Journal of Korean Academy of Nursing
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• v.48 no.1
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• pp.1-11
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• 2018

Purpose: The purpose of this study was to examine the effects of aromatherapy on stress responses, autonomic nervous system (ANS) activity, and blood pressure in patients hospitalized to receive coronary angiography (CAG). Methods: A non-equivalent control group with a pretest-posttest design was used. The subjects were patients admitted to the day angiography room to receive CAG at E University Hospital (34 in the experimental group and 30 in the control group). The experimental group treatment was inhalation of the aroma oil blended with lavender, ylang-ylang, and neroli at a ratio of 4:2:1 twice before and after CAG. The measurements of stress index, ANS activity, and blood pressure were performed 5 times as follows: at admission, at pre-CAG after treatment I, at post-CAG, 2 hours after treatment II, and 4 hours after treatment II. The data were analyzed using the Mann-Whitney U Test and repeated-measures analysis of variance. Results: Significant interactions in the high frequency of ANS (F=5.58, p=.005) were observed between group and time. Stress index (z=2.14, p=.016), systolic blood pressure (z=4.14, p<.005), and diastolic blood pressure (z=3.28, p=.001) were significantly different between the experimental and control groups after 4 hours of treatment II. Conclusion: The findings showed that aromatherapy was not effective before CAG, but was effective after CAG. Therefore, aromatherapy can be used as a nursing intervention for patients receiving CAG.

• Proceedings of the Korean Society for Emotion and Sensibility Conference
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• 2001.11a
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• pp.104-111
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• 2001

정서와 생리반응 (자율신경계 반응) 간의 관계에 관하여 성인을 대상으로 최근가지 많은 연구가 행해져 왔다. 본 연구에서는 동일한 실험참여자를 대상으로 일정 기간 동안 여러 회의 반복실험을 통해 정서(기쁨, 슬픔, 분노, 공포, 혐오)에 따른 자율신경계의 반응의 일관성과 정서별 자율신경계 반응 패턴을 규명하고자 하였다. 본 실험에 앞서 저서를 유발하기 우한 도구인 정서유발자극세트와 정서에 대한 심리반응을 평가하기 위한 정서평가척도가 제작되었다. 정서유발자극세트는 2-4분 정도의 각 정서 장면이 포함된 총 5개의 동영상 장면들이다. 예비실험을 통해 70％ 이상의 적합성 및 효과성을 가진 4개의 세트를 추출하여 본 실험에 사용하였다. 본 실험은 남녀 대학생 12명을 대상으로 4회 반복해서 실시되었다. 실험참여자들은 각 정서 장면을 시청 후, 유발된 정서에 대한 심리적인 평가를 하였다. 측정한 자율신경계 생리반응 변수는 ECG, PPG, EDA, SKT이었다. 연구 결과, 심리반응에서 정서유발자극세트는 75％ 이상의 적합성 및 효과성을 보였다. 생리반응(ECG, EDA) 분석 결과, 정서에 따른 자율신경계 반응은 회기별로 일관적이었으며, 각 정서별로 특정적인 생리반응 패턴을 가지는 것으로 나타났다.

• Proceedings of the Korean Society for Emotion and Sensibility Conference
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• 1999.11a
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• pp.216-221
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• 1999

A mental task combined with noise background is an effective model of laboratory stress for study of psychophysiology of the autonomic nervous system (ANS). The intensity of the background noise significantly affects both a subjective evaluation of experienced stress level during test and the physiological responses associated with mental load in noisy environments. Providing tests of similar difficulties we manipulated the background noise intensity as a main factor influencing a psychophysiological outcome and the analyzed reactivity along withe the noise intensity dimension. The goal of this study was to identify the patterns of ANS responses and the relevant subjective stress scores during performance of word recognition tasks on the background of white noise (WN) of the different intensities (55, 70 and 85 dB). Subjects were 27 college students (19-24 years old). BIOPAC, Grass Neurodata System and AcqKnowlwdge 3.5 software were used to record ECG, PPG, SCL, skin temperature, and respiration. Experimental manipulations were effective in producing subjective and physiological responses usually associated with stress. The results suggested that the following potential autonomic mechanisms might be involved in the mediation of the observed physiological responses: A sympathetic activation with parasympathetic withdrawal during mild 55 and 70dB noise (featured by similar profiles) and simultaneous activation of sympathetic and parasympathetic systems during intense 85dB WN. The parasympathetic activation in this case might be a compensatory effect directed to prevent sympathetic domination and to maintain optimal arousal state for the successful performance on mental stress task. It should be mentioned that obtained results partially support Gellhorn's (1960; 1970) "tuning phenomenon" as a possible mechanism underlying stress response.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.22 no.52
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• pp.203-210
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• 1999

We report on some of the preliminary results of the physiological responses resulting from vehicular speed changes. Healthy human subjects (n=5) were studied for the experiments. We measured the physiological responses of the subjects such as Heart Rate Variability (HRV), Galvanic Skin Response (GSR), and skin temperature for day and night vehicular speed change experiments, respectively. Before and after the tasks, we carried out a self-report for acquiring correlation with experiment results. Mean heart rate variability (HRV) and amplitude of GSR and skin temperature were calculated for 3 minutes duration in each state. The analysis of the physiological measures of ANS activity revealed that vehicle speed change-based affective state evoked arousal response pattern featured by HR acceleration, decrease of skin temperature, and increase of GSR amplitude. The obtained results show that despite some differences observed between each state, overall physiological responses show that the activity of the sympathetic nervous system increases as a result of the increase of speed.