• Korean Journal of Acupuncture
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• v.34 no.4
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• pp.265-270
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• 2017

Objectives : The present study aimed to examine the analgesic effect of electroacupuncture(EA) applied to the brook point of the Liver meridian in the rat model of inflammatory pain and to investigate involvement of endogenouse opioid in the EA-induced analgesia. Methods : Knee arthritis was induced by injection of $125{\mu}l$ of complete Freund's adjuvant into the knee joint cavity unilaterally. To examine the level of pain, weight bearing forces(WBFs) of affected limb was measured. EA treatment was given at the LR2, LI4 or non-acupoint on the contralateral limb with frequency of 2 Hz and intensity of 2 mA under gaseous anesthesia. Results : After induction of arthritis, rats subsequently showed a reduced stepping force of the affected hindlimb due to a painful knee. EA applied to the LR2 point on the contralateral hind limb produced a significant improvement of stepping force of the hind limb lasting for at least 2 h, and this effect was equivalent to that obtained by 5 mg/kg of oral indomethacin. The effect of EA was specific to the acupoint since it could not be mimicked by EA applied to the LI4 point or the arbitrary non-acupoint. The analgesic effect was blocked by pretreatment with naltrexone(10 mg/kg, i.p.). Conclusions : These results suggest that acupuncture applied to LR2, brook point of Liver meridian suppresses inflammatory pain in a rat model of knee arthritis and this effect seems to be mediated by endogenous opioid systems.

• The Journal of Internal Korean Medicine
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• v.10 no.1
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• pp.39-52
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• 1989

The present investigation was undertaken to confirm of clinical effects of Sagnating. So, experimental studies were done, especially, to ascertain whether Sagantang had pharmacological effect of analgesia, antipyresis, diuresis and decreasing blood pressure and so on. The results of the studies were obtained as follows: 1. The analgesic effects of Sagantang were obtained in mice. 2. Sagantang prolonged the duration of hypnosis which is induced thiopental-Na in mice, but it was not effective in rotor rod method. 3. Antipyretic effect of Sagantang was known on the typhoid vaccine induced fever in rats. 4. Sagantang inhibited automatic movement on the isolated ileum of mice, and antiacetylcholine effect and antibarium chloride effect of Sangantang were known on that. 5. Sangantang decreased blood pressure due to vasodilatation in anesthetized mice. 6. The effect of diuresis were noted in mice. According to the above results, clinical effects of Sagantang on oriental medical references were similar to the actual experimental results.

• Biomolecules & Therapeutics
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• v.2 no.4
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• pp.347-360
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• 1994

The general and some pharmacological actions of DWP 301 were investigated in animals and the following results were obtained. In central nervous system, DWP 301 had no effects on the pentobarbital induced anaesthesia, rotarod test, traction test, analgesic action, anticonvulsant action in mice and body temperature in rat. But DWP 301 showed a little decrease of locomotor activity at a dose of 3,000 mg/kg. From these results, DWP 301 was considered to have little pharmacological effect on the central nervous system. Furthermore, DWP 301 had no influences on the normal blood pressure and heart rate. DWP 301 showed no effect on the isolated guinea pig ileum, trachea, right atrium, and nonpregnant rat uterus. But, in the isolated guinea pig vas deference, DWP 301 had showed inhibitory effect on the contractions produced by norepinephrine. DWP 301 showed rise of gastric juice pH and decrease of urine volume. Also, DWP 301 had no effect on the gastrointestinal motility and blood aggregation. From these results, it is concluded that the general pharmacological effect of DWP 301 are similar to or weaker than M and AGA.

• Archives of Pharmacal Research
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• v.20 no.5
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• pp.414-419
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• 1997

Gastrointestinal irritation is the most frequent adverse effect in patients chronically taking nonsteroidal antiinflammatory drugs (NSAIDs) for the treatment of arthritic conditions. Gastroprotective effect of DA-9601, a new antiulcer agent from Artemisiae Herba extract, against NSAID was evaluated in a rat model of arthritis that is similar in many aspects to human rheumatoid arthritis. Daily oral dosing of naproxen (30 mg/kg), one of the most commonly used NSAID, induced apparent gastric lesions as well as a significant decrease in mucosal prostagiandin $E_2;(PGE_2)$ and prostagiandin F_${1{\alpha}}$$(PGF_{1{\alpha}})$ levels. Coadministration of DA-9601 prevents naproxen-induced mucosal injury and depletion of prostaglandins, in a dose-related manner. DA-9601 did not alter the antiinflammatory or analgesic effect of naproxen. The present results suggest that DA-9601 may be useful as a mucoprotectant against NSAIDs in clinical practice.

• Korean Journal of Veterinary Research
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• v.28 no.1
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• pp.37-47
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• 1988

Xylazine hydrochloride is a widely used analgesic, sedative and muscle relaxant agent in veterinary clinic. Yohimbine hydrochloride and 4-aminopyridine are known as antagonists of xylazine hydrochloride. This paper was investigated to know that the effect of xylazine hydrochloride, yohimbine hydrochloride and 4-aminopyridine, and that whether or not antagonism of yohimbine hydrochloride and 4-aminopyridine to xylazine hydrochloride-induced effect on gizzard motility in chicken. The results were as follows. 1. After xylazine hydrochloride administration, the gizzard motility in chicken was instantly inhibited in relaxation state, and this state was prolonged in proportion to increase of dose. 2. After yohimbine hydrochloride administration, the gizzard motility in chicken showed increase of contractile frequency. 3. After 4-aminopyridine administration, the gizzard motility in chicken was gradually recovered next to decrease of contractile amplitude and frequency. 4. After the combination of yohimbine hydrochloride and 4-aminopyridine administration, the gizzard motility in chicken showed increase of amplitude and radical increase of frequency. 5. After xylazine hydrochloride administration, the relaxation time was shortened by yohimbine hydrochloride, 4-aminopyridine and the combination of yohimbine hydrochloride and 4-aminopyridine. In conclusion, the gizzard motility in chicken was inhibited by xylazine hydrochloride, and this effect was antagonized by the combination of yohimbine hydrochloride and 4-aminopyridine.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.1 no.1
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• pp.15-24
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• 1988

To study the effect of Sopoungwhalwheol-tang and Sopoungwhalwheol-tang with cheon chung on the "Tong poung" "yeouk Geul poung" inoriental medicine (Rheumatoid arthrits) measure cure effect on the Analgesic, Anti-inflammatory anti-phlogistic and concentration of albumin's Test. The results are Summarized as follows, 1. Sopoungwhalwheol-tang liquid reduced significantly the pain by acetic acid in accordance with the increase of double dosages, and Sopoungwhalwheol-tang with cheon chung (which was similar with Aspring Group) was more marked effective. 2. Sopoungwhalwheol-tang with cheon chung reduced the edema by carragenine and at 60 minutes, two groups all reduced the edema and at 180 minutes, Sopoungwhalwheol-tang was recognized some effectiveness and Sopoungwhalwheol-tang with cheon chung was recognized excellent effectiveness. 3. Sopoungwhalwheol-tang with cheon chung has more continued Antiphlogistic effect against the fever by yeast which was fallen after 60 minutes of prescription of medicine than Sopoungwhalwheol-tang. 4. Sopoungwhalwheol-tang with cheon chung was more effective against the concentration of albumin by $1\%$ bovine serum albumin than Sopoungwhalwheol-tang. 5. According to the above experiments. Sopoungwhalwheol-tang with cheon chung has greater anti-inflammatory, antiphlogistic Analgestic effect than Sopoungwhalwheol-tang.

• Biomolecules & Therapeutics
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• v.5 no.2
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• pp.150-157
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• 1997

Capsaicin cream has been used to attenuate the pain associated with diabetic neuropathy, rheum-atoid arthritis, osteoarthritis and postherpetic neuralgia. But its common side effect, local irritation, limits the use of it and there is still a need for a new analgesic devoid of this side effect. This study was conducted to compare the local irritant effect of DA-5018, a new capsaicin derivative, with that of capsaicin in various animal models and human beings. Capsaicin, applied topically to the mouse ear, produced dose-dependent increase of ear volume and the frequency of ear scratching behavior in mice. Neither ear volume nor scratching behavior was affected by DA-5018. In eye wiping test of rat, DA-5018 was 10 times less irritant than capsaicin. Capsaicin administered intradermally into the rat paw elicited paw lick/lift response with a potency which was three times that of DA-5018. Zostrix-HP (0.075% capsaicin cream), but not DA-50180.3% cream, increased ear volume of rat and induced thermal hyperalgesia in normal and carrageenan inflamed paws. Six day-treatment of Zostrix-HP failed to develop tolerance against this thermal hyperalgesia. In human beings, Zostrix-HP produced burning sensation and itching in more than 90% of volunteers involved and its maximum irritant effect was significantly higher than that of DA-5018 cream. These results suggest that local irritation and burning sensation produced by DA-5018 is much less than capsaicin.

• Korean Journal of Plant Resources
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• v.23 no.6
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• pp.513-518
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• 2010

In the present study, the antinociceptive profiles of Dianthus chinensis L extract were examined in ICR mice. Dianthus chinensis L extract administered orally (200 mg/kg) showed an antinociceptive effect as measured by the tail-flick and hot-plate tests. In addition, Dianthus chinensis L extract attenuated the writhing numbers in the acetic acid-induced writhing test. Furthermore, the cumulative nociceptive response time for intrathecal (i.t.) injection of substance P ($0.7\;{\mu}g$) was diminished by Dianthus chinensis L extract. Intraperitoneal (i.p.) pretreatment with yohimbine ($\alpha_2$-adrenergic receptor antagonist) attenuated antinociceptive effect induced by Dianthus chinensis L extract in the writhing test. However, naloxone (opioid receptor antagonist) or methysergide (5-HT serotonergic receptor antagonist) did not affect antinociception induced by Dianthus chinensis L extract in the writhing test. Our results suggest that Dianthus chinensis L extract shows an antinociceptive property in various pain models. Furthermore, this antinociceptive effect of Dianthus chinensis L extract may be mediated by $\alpha_2$-adrenergic receptor, but not opioidergic and serotonergic receptors.

• The Journal of Korean Physical Therapy
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• v.15 no.4
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• pp.299-311
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• 2003

The purpose of this study is to investigate and analysis effect of TENS with immunohistochemistry methode through changes of substance P in spinal using arthritis model after inducing inflammation. The changes of substance P induced at that time are compared with control which is not induced arthritis by means of counting. The effect of TENS (4Hz, $200{\mu}$, 20minutes) is also tested by observing changes of substance P in spinal dorsal horn after application on knee joint of rats which is arthritis model induced by kaolin and carrageenan. The results of this study were as follows: 1. Substance P immunoreactive positive neurons are increased in dorsal horn after inducting arthritis. 2. In arthritis group, Substance P immunoreactive positive neurons are progressively increased from the first to the third days. 3. Substance P immunoreactive positive neurons after applicating TENS on arthritis group are more decreased than only arthritis-induced group. 4. Substance P immunoreactive positive neurons were significantly decreased on the second days resulting from TENS application from the first to the third days. Therefore, TENS application is decrease Substance P immunoreactive positive neurons in spinal dorsal horn of rats induced arthritis. This decrease is considered as analgesic effect of TENS.

• Journal of Veterinary Clinics
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• v.15 no.2
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• pp.394-398
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• 1998

This study was performed to assess clinical signs, sedative effect and clinicschemical profile of a mixture of fentanyl-azapemne-xylazine(Fentazine$^{(R)}$) in formed elk Twelve male elk(Cervus canadensis) were immobilized with Fentazine, and blood samples were taken of femoral venous blood. Samples were analyzed in the conditions of 10- and 30-minute after administration of the drug. Heart rates, respiratory rates, and body temperatures were in normal ranges during Fentazine anesthesia. After iqiection of Fentazine, most of elk were recumbency and did not respond to needle prick In young adult(3.5.4.5 years old) elk a high dose(>3.0 ml/head) of Fentazine does not result in more sedation, but it does prolong the duration of sedative effect. Fentazine induced sufficient analgesia far velvet antler removal and hoof trimming in elk Salivation, urination, intermittent apnea and mild bloat were observed in elk Globulin, alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, calcium, magnesium and phosphorus values were in normal ranges for at least 30 minutes after Fentazine administration. Total protein, albumins cortisol and prothrombin values were slightly increased during sedation(p<0.05). It was concluded that Fentazine is effective analgesic drug being useful for velvet antler removal and hoof trimming.