• Korean Journal of Food Science and Technology
• /
• v.17 no.6
• /
• pp.495-499
• /
• 1985

To develope a process by which liquid foods can be stored in the liquid state at the frozen storage temperature, suitable cryoprotectants were selected. Orange juice, chosen as an example of liquid foods, was stored with combined cryoprotectants at $-15^{\circ}C$, and quality changes of orange juice during storage were evaluated. Among 7 cryoprotectants tested, NaCl solution had lower initial freezing point than others, and initial freezing points of glucose, fructose, glycerol, propylene glycol and citric acid were close to each other. Considering flavor quality of orange juice, cryoprotectants suitable for reducing freezing point of orange juice were glucose, fructose, glycerol, and citric acid. Combined cryoprotectants for reducing freezing point of 3 and 4 folds concentrated orange juice to $-15^{\circ}C$ consisted of 10% glucose, 8% frutose, 4.6% glycerol and 3% citric acid, and 5.5% glucose, 4.5% fructose, 4.6% glycerol, and 3% cirtric acid, respectively. When destruction of ascorbic acid, sedimentation volume and sensory flavor score of orange juice stored with combined cryoprotectants at $-15^{\circ}C$ and the control stored at $-18^{\circ}C$ were compared, there were no significant differences. These results indicated that liquid foods with suitable combined cryoprotectants could be stored at $-15^{\circ}C$ or below in the liquid state without adverse effect on quality of the stored products.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.24 no.4
• /
• pp.341-346
• /
• 2004

To determine lethal temperature of birdsfoot trefoil(BFT) and italian ryegrass(IRG) at heat-stressed conditions, seedlings grown in a small pots fur 4 weeks were subjected to different temperature regimes of heat treatment. No apparent damage was observed BFT and IRG were treated at 45, 50 or $60^{\circ}C$ for 1 h. And also heat treatments at 60, 65 and $70^{\circ}C$ for 1 h, both of them were withered and showed damage symptom on their leaves but it was not lethal conditions for the whole plants. By contrast, most of plants were prominently withered within one day after heat treatment at $80^{\circ}C/60min$. When BFT was exposed to $80^{\circ}C/60$ min, they were died within 6 days but there was found that new shoots were regenerated from the plants that had been treated at $80^{\circ}C$ within 55 min. IRC was also died within 2 days that exposed to $80^{\circ}C/20$ min but there was found that new shoots were regenerated from the plants that had been treated at $80^{\circ}C$ within 15 min. These results indicate that heat killing temperatures of BFT and IRG plants are $80^{\circ}C/60$ min an $80^{\circ}C/20$ min respectively. Simple viability assay system established in this study will be useful for selection and characterization of heat-tolerant transgenic BFT and IRG plants.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.25 no.4
• /
• pp.251-258
• /
• 2005

This study was conducted to examine the effect of application of pig manure slurry(PMS) for rye cultivation in Paddy-land on rice productivity The field experiments were carried out from 1999 to 2002 on well-grown rice in Paddy-land at Kimje, Chunbuk province in Korea. PMS was manufactured by the fermentation for 6 months in natural condition. Amount of PMS was adjusted equal to total nitrogen value relative to its value of commercial chemical fertilizers. The amount of PMS were spread evenly over the soil surface two times; first in the autumn (before sowing, end of November) and second in the spring(regrowth of rye, middle of March). The field plots were consisted of four kinds (PMS $100\%$ treatment, PMS $200\%$ treatment PMS $100\%$ treatment with half of CF (CF $50\%$), Full of CF treatment (CF $100\%$). The experiment was designed as a randomized complete block. Each treatment was replicated three times. Rices are customarily gown under tropical korean condition as annuals. The results obtained from this study were summarized as fellows. Panicle length of rice was shorter in PMS $100\%$ than other treatments. Ripening grain ratio was the lower in CF treatment. In treatment of PMS $100\%$ and PMS $200\%$, 1000 grain weight was a downward trend as compared to that of full of CF treatments, whereas number of grains per $m^2$ was increased as compared to other treatments. Number of panicles reveals that there is an increase in order; CF $50\% + PMS\;100\%>\;PMS\;100\%\;>\;CF\;100\%\;>\;PMS\;200\%$. Yield of milled rice showed hi인 in CF $100\%$ and $CF 50\%+ PMS 100\%$, but were was no significant difference among treatments. There were did not a regular tendency in the ratio of leaf sheath bright, however the ratio of lodging area was higher in $CF50\%+PMS\;100\%$ treatment than other treatments. Based on the results of this experiment, $CF 50\%+PMS\;100\%$ may be the most effective in rice productivity under application of PMS for rye cultivation in Paddy-land.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.25 no.4
• /
• pp.307-318
• /
• 2005

This study was conducted to examine effects of brewery meal-based fermented feedstuff supplemented with Aspergillus oryzae(AO) or Saccharomyces cerevisiae(SC) on luminal micro-organism of Korean native cattle. Two cows equipped with luminal cannulas were used as experimental animals. Experiment was done with three treatment groups: $71.5\%$ of commercial feed and $28.5\%$ of com silage(control): $45.0\%$ of commercial feed, $26.5\%$ of fermented feedstuff supplemented with AO and $28.5\%$ of corn silage(TAO): $45.0\%$ of commercial feed, $26.5\%$ of fermented ffedstuff supplemented with SC and $28.5\%$ of corn silage(TSC). The number of total viable bacteria (p<0.05), anaerobic fungi and protozoa(p<0.05) was higher in TAO and TSC than in control. The number of proteolytic bacteria(p<0.05), cellulolytic bacteria and xylan fermenters tended to be higher in TAO and TSC than in control. The dry matter recovery (DMR) of protozoa was higher in TAO and TSC than in control(p<0.05). The crude protein (CP) content of total microbes and protozoa was higher in TSC than in control and TAO (p<0.05). The CP content of bacteria was higher in TAO and TSC than in control(p<0.05). The ether extract(EE) content of the total microbes was higher in TAO than in control and TSC(p<0.05), and the EE of protozoa and bacteria were higher in TSC than in control and TAO(p<0.05). The ratio of essential amino acids of total microbe was higher in control than in TAO and TSC(p<0.05). The ratio of methionine and alanine of bacteria was higher in TAO and TSC than in control(p<0.05). The results suggested that the feeding of fermented feedstuff supplemented with AO or SC had an influence on the numbers of ruminal microorganism and the changes of microbial body composition.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.22 no.1
• /
• pp.69-76
• /
• 2002

This pot experiment was conducted to find out the forage productivity and contents of mineral nutrients in an orchardgrass sward as affected by the compost lime, and phosphorus applications on the newly reclaimed and arable pasture soils. This 1st part was concerned with the effect of compost application. The results obtained are summarized as follows: 1. There were considerable differences between the newly reclaimed and arable pasture soils in the soil chemical properties as fellows(newly reclaimed soil ↔ arable pasture soil): poor ↔common in pH and contents of Ca and Mg; Poor ↔ good in content of available P$_2$O$_{5}$, base saturation, and mutual balance of Ca : Mg : K; common ↔ good in K content; good ↔ good in absorption coefficient of P$_2$O$_{5}$; poor ↔ somewhat poor in CEC. The contents of available P$_2$O$_{5}$ in both soils after experiment were relatively higher in the compost block than non-compost block. It was recognized that the compost application was resulted in the CEC increase in the newly reclaimed soil. 2. The vigour at early growth of orchardgrass was better in the arable pasture soil then newly reclaimed soil. In the newly reclaimed soil, the vigour at early growth of orchardgrass was remarkedly enhanced by compost application. 3. Under the non-compost application, the orchardgrass yield was higher in the arable pasture soil by 50.9% than newly reclaimed soil. Compared with the non-compost application, the orchardgrass yields were increased in the newly reclaimed soil by 17.1% and the arable pasture soil by 7.6% by the compost application. 4. The concentrations of Ca, Mg, and P in orchardgrass were relatively higher in the arable pasture soil than the newly reclaimed soil, whereas there was no difference in the K concentration. It was recognized that the concentrations of Mg and P in orchardgrass were higher in the compost block than the non-compost block.block.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.20 no.1
• /
• pp.67-76
• /
• 2000

An experiment was conducted to evaluate the effects of lime, zeolite, and rock phosphate as coating materials and coated seed size on germination, establishment, and early growth of pasture species at the experimental livestock farm, Yonam College of Agriculture in 1997. Germinating energy and germination of coated tall fescue and orchardgrass seeds were lower than those of non-coated seeds. Coated seed of tall fescue with 70% lime and 15% rock phosphate was the best in germination. The germination of small size seed (< ${\varnothing}2$ mm) was better than those of medium (> ${\varnothing}2$ mm and < ${\varnothing}2$ mm) and large size seeds (> ${\varnothing}2$ mm). Germination of coated orchardgrass seed with 55% lime and 30% rock phosphate treatment was higher than that of other coated seeds, and germination of small size seed was higher than those of medium and large size seeds (p<0.05). Germination energy and germination of coated alfalfa and birdsfoot trefoil seeds were lower than those of non-coated seeds. Germination of coated alfalfa seed with 70% lime and 15% rock phosphate, and 45% lime and 55% rock phosphate was higher compared with other coated seeds. Germination of medium size seed was higher than those of small and large size seeds (p<0.05). Among coated birdsfoot trefoil seed, 45% lime and 40% rock phosphate treatment resulted in higher germinating energy and germination than other treatments. There was no significant difference in establishment and early growth of coated tall fescue and orchardgrass seeds. However, tall fescue and orchardgrass coated with 45 % lime and 40% phosphate rock showed higher establishment and early growth. There was also no significant difference in establishment and early growth of coated alfalfa among the treatments. Among coated treatments, establishment and early growth of alfalfa coated with 75% lime and 10% rock phosphate were the highest and showed 46.4% and $72.6\;g/\textrm{m}^2$, respectively. Establishment and early growth of coated birdsfoot trefoil with 55% lime and 30% rock phosphate were the highest among coated birdsfoot trefoil seed (p<0.05) and recorded 46.4% and $44.6\;g/\textrm{m}^2$, respectively. Results of this study indicate that the germination of coated grass and legume seed may be improved by increasing the percentage of rock phosphate and decreasing that of lime. With regard to seed size, the germination of pasture species with small seed size (< ${\varnothing}2$ mm) may be better than medium and large size seeds except alfalfa. Establishment and early growth of grasses can be also improved by increasing the percentage of rock phosphate and decreasing that of lime.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.6 no.3
• /
• pp.157-163
• /
• 1986

Synthesis and accumulation pattern of Weender components in orchardgrass (Dactylis glomerata L.) cv. Potomac and Baraula, perennial ryegrass (Lolium perenne L.) cv. Reveille and Semperweide and meadow fescue (Festuca pratensis Huds.) cv. Cosmos 11 and N.F.G. were studied under different growth environments and cutting managements. The field experiments were conducted as a split plot design with three cutting regimes of 6-7 cuts at grazing stage, 4-5 cuts at silage and 3 cuts at hay stage from 1975 to 1979 in Korea and West Germany. The results obtained are summarized as follows: 1. Air temperature, rainfalls and solar radiation were found to be an important meteorological factors influenced to synthesis and accumulation of Weender components. Under high temperature and strong solar radiation during summer season in Korea, accumulation of crude fiber and cell-wall constituents (NDF) in the plants, as average of all grass species and cutting regimes, were increased to about 30.1% and 48.7% from 27,9% and 42.9% in spring, respectively, while total nonstructural carbohydrates (TNC) were decreased to 1.52% in summer from 4.01% in spring. In West Germany, the concentration of Weeder components showed little seasonal variation. 2. Crude fiber and neutral detergent fiber (NDF) were shown higher concentration in orchardgrass than those of perennial ryegrass and meadow fescue, but N-free extractions and TNC as well as net energy value were less accumulated in orchardgrass. Orchardgrass contained lower net energy contents with 534 StE. 431 StE and 575 StE/kg for Suweon, Cheju and Freising, respectively, as compared with 624 StE (Suweon), 491 StE (Cheju) and 657 StE/kg (Freising) in meadow fescue.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.6 no.3
• /
• pp.145-150
• /
• 1986

The experiments were conducted to study the influence of growth location and cutting microelements macro-and on managements in temperate grasses in Korea and West Germany from 1975 to 1979. The field trials were designed as split plot with three grass species of Dactylis glomerata L., Lolium perenne L. and Festuca pratensis Huds under three cutting regimes at grazing stage, silage stage and hay stage. The results obtained are summarized as follows: 1. Concentrations of macro-and microelements in temperate grasses showed a different response to growth location and growing season. P concentration in the plants was decreased under hot stress in summer, whereas Mg and Na tended to be increased. The seasonal changes in K and Zn were not significant. 2. Morphological growth stage was to be found as an important factors influenced to mineral components. P and K contents in temperate grasses tended to be decreased as morphological development especially under high temperature in Suweon and Cheju. Ca and Mg were less affected by morphological stage and cutting managements. 3. Mean value of Ca/P ratio in the plants were 1.58, 1.33 and 1.21 for meadow fescue, perennial ryegrass and orchardgrass, respectively. Ca/P ratio in grasses tended to be increased as morphological development. 4. Zn deficiency in the plants occured in all grass species and experimental sites. Mean Zn concentration of the plant were 34.2%, 31.2% and 37.8% for Suweon, Cheju and Taekwalyong, respectivelly. Na deficiency occured in orchardgrass and meadow fescue, especially in taekwalyong. Cool temperature resulted in a decrease of Na absorption and accumulation.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.9 no.1
• /
• pp.26-33
• /
• 1989

This pot experiments were conducted to find out the optimal fertilization ratios of the N:S:P anions and K:Ca:Mg cations of major nutrients in an orchardgrass/ladino clover mixed sward. The optimum ratios in equivalent basis were computed by the Homes systematic variations technique. The soil samples were collected from the newly reclaimed soils, which are located on colluvial hilly area with a good consideration for the hilly pasture development. The results were summarized as follows; 1, the optimum fertilization ratios of major nutrients for the high yields by the forage species in a mixed sward were obtained (Table 4 in detail); N:S:P = 3:l: 1 and K:Ca:Mg = 1:l: 1 for grass and grass plus legume, and N:S:P = 1:6:43 and K:Ca:Mg = 1: 3: 1 for legume in general. 2. The yield increases from the systematic variations in this mixed sward were laid in following order; N > P >S-group and K >Mg >Ca-group for grass and grass plus legume, and P >S >N-group and Ca >Mg > K-group for legume. Especially, the yields were greatly increased at the N-group for grass, and at the Pand Ca-groups for legume. 3. Soil pH-value was descreased at the Sgroup, and somewhat increased at the Ca- and Mg-groups. The content of available $P_2O_5$, CEC and base saturation were greatest with the Ca-group. 4. At the N-group, the N-contents were highest and the P-contents were lowest in grass and grass plus legume, which resulted in the highest Ca/P ratio of 2.15 among the anion groups. Whereas the highest Ca/P ratio of 9.20 in legume was obtained at the Ca-group. Legume showed in general higher Ca/P ratio and lower K/(Ca+Mg) ratio than these in grass. 5 . There were differences in the effects of systematic variations of major nutrients on the dry matter yields and the mineral yields. The optimum fertilization ratios of anions and cations for the high mineral yields were obtained (Table E), which showed differences comparing with the ratios for the high dry matter yields. The antagonis between the cations K and Mg was known from the point of mineral yields of mixed forages.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.9 no.1
• /
• pp.7-14
• /
• 1989

This experiment was carried out to determine the seasonal herbage production and utilization during the growing season of pasture on the forest (shading 30%). Plant height, leaf area index(LAl), dry matter(DM) production and distribution, chemical composition, in vitro dry matter digestibility(IVDMD), herbage utilization percentage and chewing efficiency were investigated using the Corridale sheep. Experimental field was treated by one plot design(3 rep.) and performed from 1987 to 1988 at Chungnam National University, Daejon. The results obtained are summarized as follows: 1. The highest plant height and LA1 were observed in May(35.0 cm, 4.89), followed by April(28.0 cm, 4.23), while the plant height and LA1 in October (13.0 cm, 0.49) showed very low. 2. During the growing season, about 58.3 % of annual DM production (7240 kg/ha) was produced during the spring (April, May and June) and the highest DM production was obtained in May (2040 kg/ha), which was more than 28.2 % of total DM production. However, DM production in July and August was about 24.2 % and those in September and October (17.5 %) was very low, but the difference of DM production from June to September was small. 3. The maximum DM production per day (65.8 kg/ha) was observed in May, followed by June (28.7 kglha), while DM production per day in October (16.5 kg/ha) showed very low (p <0.01). 4. Crude protein content and IVDMD of herbage samples during the spring (April, May and June) were higher, while crude fiber, ADF, and NDF content were lower in an summer growth herbage samples (July and August), but autumn growth herbage samples was intermediate. Crude ash content and IVDMD of collected herbage samples were slightly more, while crude fiber, ADF and NDF content were slightly less than offered and residued herbage samples during the growing season. 5. The maximum DM intake per metabolic body size was observed in May(68.9 g), followed by October (66.7 g), while very low in August (52.5 g). Significant positive correlation (p <0.05) was found between DM intake and IVDMD. 6. Herbage utilization percentage was very high in April (83.4 %), while very low in August (64.0 %). The percentage of annual herbage utilization was about 75.5 %. 7. The maximum ruminating and chewing efficiency of herbage samples were observed in May, followed by October, while very low in August.