• Title/Summary/Keyword: AMV

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Biological and Molecular Variability of Alfalfa mosaic virus Affecting Alfalfa Crop in Riyadh Region

  • AL-Saleh, Mohammed A.;Amer, Mahmoud A.
    • The Plant Pathology Journal
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    • v.29 no.4
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    • pp.410-417
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    • 2013
  • In 2011-2012, sixty nine samples were collected from alfalfa plants showing viral infection symptoms in Riyadh region. Mechanical inoculation with sap prepared from two collected samples out of twenty five possitive for Alfalfa mosaic virus (AMV) by ELISA were produced systemic mosaic on Vigna unguiculata and Nicotiana tabacum, local lesion on Chenopodium amaranticolor and C. quinoa. Vicia faba indicator plants that induce mosaic and mottle with AMV-Sagir isolate and no infection with AMV-Wadi aldawasser isolate. Approximately 700-bp was formed by RT-PCR using AMV coat protein specific primer. Samples from infected alfalfa gave positive results, while healthy plant gave negative result using dot blot hybridization assay. The nucleotide sequences of the Saudi isolates were compared with corresponding viral nucleotide sequences reported in GenBank. The obtained results showed that the AMV from Australia, Brazil, Puglia and China had the highest similarity with AMV-Sajer isolate. While, the AMV from Spain and New Zealaland had the lowest similarity with AMV-Sajer and Wadi aldawasser isolates. The data obtained in this study has been deposited in the GenBank under the accession numbers KC434083 and KC434084 for AMV-Sajer and AMV-Wadialdawasser respectively. This is the first report regarding the gnetic make up of AMV in Saudi Arabia.

UNCERTAINTIES IN AMV ESTIMATION

  • Sohn, Eun-Ha;Cho, Hee-Je;Ou, Mi-Lim;Kim, Yoon-Jae
    • Proceedings of the KSRS Conference
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    • 2007.10a
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    • pp.153-155
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    • 2007
  • Korea Meteorological Administration (KMA) has operationally produced Atmospheric Motion Vector (AMV) from the consecutive MTSAT-1R satellite image dataset. Comparing with radiosonde data, our current AMV scheme shows more than 10 m/s RMSE. Therefore we need to improve continuously its accuracy. Many AMV producers have stated that the bad performance of the Height Assignment (HA) algorithm is the main reason of degrading the accuracy of AMV. The uncertainties in AMV HA can occur in the algorithm itself, used NWP profiles, and the performance of Radiative Transfer Model (RTM) etc. This study introduces currently operated AMV HA schemes and the impacts of NWP profile data and RTM that these schemes use were investigated. Finally we analyzed the relationship between vectors by vector tracking and heights assigned to each vector by using collocated wind profile dataset with radiosonde data. This study is a preliminary work to improve the accuracy of AMV by removing or decreasing the uncertainties in AMV estimation.

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AMV: A k-anonymization technique minimizing the cloaking region (AMV: 클로킹 영역을 최소화하는 k-익명화 기법)

  • Song, Doohee;Heo, Minjae;Sim, Jongwon;Hwang, Sori;Song, Moonbae;Park, Kwangjin
    • Journal of Internet Computing and Services
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    • v.15 no.6
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    • pp.9-14
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    • 2014
  • In this paper, we propose AMV scheme which supports k-anonymization by using vectors for mobile clients. AMV can produces the minimal cloaking area using motion vector information of users (clients). The main reason for minimizing cloaking area is a server has to send the object information to all users who request the spatial queries. The experimental results show that the proposed AMV has superior performance over existing methods.

Effects of Amomum villosum(AMV) Extract on the Alzheimer's Disease Model (사인(砂仁)이 Alzheimer's Disease 병태 모델에 미치는 영향)

  • Choi Bo-Yun;Jung In-Chul;Lee Sang-Ryong
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.20 no.1
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    • pp.43-51
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    • 2006
  • This experiment was designed to investigate the effect of Amomum villosum(AMV) on the Alzheimer's disease. The effects of AMV extract on amyloid precursor proteins(APP), acetylcholinesterase(AChE), glial fibrillary acidic protein(GFAP) mRNA of PC-12 cell line treated by amyloid $\beta$ protein($A{\beta}$) : IL-$1{\beta}$, IL-6, TNF-$\alpha$ mRNA of THP-1 cell line treated by lipopolysaccharide(LPS) : AChE activity of PC-12 cell lysate treated by $A{\beta}$ : serum glucose, uric acid, AChE activity of memory deficit rats induced by scopolamine : behavior of memory deficit mice induced by scopolamine were investigated, respectively. AMV extract suppressed APP, AChE, GFAP mRNA in PC-12 cell treated by $A{\beta}$ : IL-$1{\beta}$, IL-6, TNF-$\alpha$ mRNA in THP-1 cell treated by LPS , AChE activity in cell lysate of PC-12 cell treated by $A{\beta}$. AMV extract increased glucose, decreased uric acid and AChE significantly in the serum of the memory deficit rats induced by scopolamine. AMV extract group showed significantly inhibitory effect on the memory deficit of mice induced by scopolamine in the experiment of Morris water maze. According to the above results, it is suggested that AMV extract might be usefully applied for prevention and treatment of Alzheimer's disease.

Incidence and Occurrence Pattern of Viruses in Lilies (Lilium spp.) on Jeju Island (제주지역 백합에서의 바이러스 발생 현황)

  • Kim, Hyo Jeong;Song, Jeong Heub;Song, MinA;Lee, Kwang Ju;Ko, Yoon Jeong;Park, Jeong Hoon;Yang, Young Taek;Heo, Tae Hyeon
    • Research in Plant Disease
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    • v.25 no.2
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    • pp.79-83
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    • 2019
  • To investigate the incidence status of lily viruses on Jeju island, lily samples were collected from 2015 to 2018 and examined for virus infection using RT-PCR. Of the viral infections, mixed and single infections were 70.0% and 17.9%, respectively. The incidence of mixed infections was highest for PlAMV and LSV as 43.4% in 2015; PlAMV, LSV 33.1% in 2016; LSV, LMoV 10.2% in 2017; and PlAMV, LSV, LMoV and CMV 15.8% in 2018. The incidence of PlAMV was observed to be 82.0% in 2015, 49.4% in 2016, 13.6% in 2017, and 39.5% in 2018 after the first occurrence of PlAMV in 2013. No symptoms were observed for single infection with LSV. However, in the case of mixed infection with LSV and LMoV, mosaic and leaf malformation symptoms appeared. With mixed infection with LSV and CMV, pale brown necrotic spots appeared, and mosaic and leaf curling were induced. PlAMV was more common in mixed infection than in single infection, and caused necrosis following the development of reddish-brown spots. PlAMV significantly decreased the marketability of lilies owing to the generation of leaf anomalies and curls, and its symptoms were more severe in mixed infections.

Identification of Virus from Azuki Bean Plant (팥에 발생하는 바이러스 분리 동정)

  • 허남기;강문석;하건수;김혜자;최장경
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.2
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    • pp.160-165
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    • 1997
  • Three types of virus disease symptoms were observed in azuki bean plants: yellow mosaic; mosaic; severe mosaic with dwarf. The symptoms developed in the indicator plant inoculated with a virus- infected leaf of azuki bean showed similar host range with those of AMV, CMV and AzMV. In antiserum response, yellow mosaic symptom formed sediments with AMV antiserum, mosaic type with CMV antiserum, respectively, From the electron microscope observation, eclliptic particle (18~58$\times$18nm), isometric particle (30nm), and filamentous(730$\times$12nm) combined with inclusion body were observed in yellow mosaic, mosaic, and severe mosaic with leaf curling symptoms, respectively, The results demonstrate that yellow mosaic, mosaic, and severe mosaic with dwarf are caused by AMV, CMV and AzMV.

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Serological Investigation of Virus Diseases of Pepper Plant (Capsicum annum L.) in Korea (혈청학적 방법에 의한 고추의 바이러스병 감염상 조사)

  • 라용준
    • Journal of Plant Biology
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    • v.15 no.1
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    • pp.23-27
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    • 1972
  • A total of 163 virus infected pepper plants(Capsicum annuum L.) collected from various pepper growing regions in Korea were investigated on the presence of tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), potato virus X(PVX), potato virus Y(PVY) and alfalfa mosaic virus (AMV) by serological methods. Van Slogteren's microprecipitin test was applied for the testing of TMV, PVX and PVY from infected plants, and Ouchterlony agar double diffusion test was used for CMV and AMV. Results obtained are as follows: 1. TMV, CMV, PVX, PVY and AMV were found to occur on the pepper plants growing in Korea. 2. The prevalence of each of these viruses among the 163 pepper plants investigated was in the order of CMV: 93 plants(57.0%)>TMV: 91 plants (55.8%)>AMV: 58 plants (35.6%)>PVY: 40 plants (24.5%)> PVX:6 plants(3.7%). 3. Among the 163 plants investigated, 72 plants (44%) showed infection with one kind of virus and 91 plants (56%) showed mixed infection with more than two different viruses. In general, heavier damage of the plants was observed from mixed infection. 4. The results of serological identification of pepper viruses coincided with those results obtained by sap inoculation experiment conducted at the Horticultural Experiment Station along with present investigation. Thus the serological techniques applied in this experiment proved to be very reliable for the identification of TMV, CMV, PVX, PVY and AMV from pepper plants infected with these viruses.

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Development and Analysis of COMS AMV Target Tracking Algorithm using Gaussian Cluster Analysis (가우시안 군집분석을 이용한 천리안 위성의 대기운동벡터 표적추적 알고리듬 개발 및 분석)

  • Oh, Yurim;Kim, Jae Hwan;Park, Hyungmin;Baek, Kanghyun
    • Korean Journal of Remote Sensing
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    • v.31 no.6
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    • pp.531-548
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    • 2015
  • Atmospheric Motion Vector (AMV) from satellite images have shown Slow Speed Bias (SSB) in comparison with rawinsonde. The causes of SSB are originated from tracking, selection, and height assignment error, which is known to be the leading error. However, recent works have shown that height assignment error cannot be fully explained the cause of SSB. This paper attempts a new approach to examine the possibility of SSB reduction of COMS AMV by using a new target tracking algorithm. Tracking error can be caused by averaging of various wind patterns within a target and changing of cloud shape in searching process over time. To overcome this problem, Gaussian Mixture Model (GMM) has been adopted to extract the coldest cluster as target since the shape of such target is less subject to transformation. Then, an image filtering scheme is applied to weigh more on the selected coldest pixels than the other, which makes it easy to track the target. When AMV derived from our algorithm with sum of squared distance method and current COMS are compared with rawindsonde, our products show noticeable improvement over COMS products in mean wind speed by an increase of $2.7ms^{-1}$ and SSB reduction by 29%. However, the statistics regarding the bias show negative impact for mid/low level with our algorithm, and the number of vectors are reduced by 40% relative to COMS. Therefore, further study is required to improve accuracy for mid/low level winds and increase the number of AMV vectors.

Inhibitory Activities of Tannins against Reverse Transcriptase and HIV-1 Replication (수종 탄닌의 역전사효소와 HIV-1 복제 저해활성)

  • 김영호;이성우;김항섭;이승호;송만기;성영철;이정준
    • YAKHAK HOEJI
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    • v.39 no.5
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    • pp.560-564
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    • 1995
  • Thirty ellagitannins originated from some Euphorbiaceous plants were tested for the inhibitory activities against AMV reverse transcriptase and replication of HIV-1 using syncytia forming assay. Most of ellagitannins showed strong inhibitory activities against AMV reverse transcriptase. Some ellagitannins including geraniin, mallotusinin, elaeocarpusin, euphorscopin and jolkianin, showed significant inhibitory activities of syncytia formation of supT1 cell line.

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Identification of Alfalfa Mosaic Virus from Soybean (대두에서 발생한 알파파 모자이크 바이러스의 분류동정에 관한 연구)

  • Lee S. H.;Choi Y. M.;Kim J. S.;Chung B. J.
    • Korean Journal Plant Pathology
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    • v.1 no.1
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    • pp.33-37
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    • 1985
  • A yellow stripe and bud benting disease of soybean was commonly observed on the field at Suweon area. The causal agent was identified as alfalfa mosaic virus (AMV) by indicator plant reactions, physical properties, serological test and electron microscopy. AMV produced vein clearing, top necrosis, top bent and mottling on the parts of soybean plants. Local lesions were produced on the inoculated leaves of Vigna sesquipedialis, Vicia faba and Tetragonia expansa, while Chenopodium am, anticolor, C. quinoa, Pisum satvium, Petunia hybrida and Nicotiana tabacum 'Bright yellow' were systemically infected. The thermal inactivation point was $60^{\circ}C$, dilution end point was $10^{-3}$, and longevity in vitro was 2 days at room temperature. AMV from soybean was reacted with AMV - antiserum in agar gel diffusion test. Electron microscopy of AMV from soybean exhibited bacilliform particles of 60nm in length.

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