• 동국한의학연구소논문집
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• 제4권
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• pp.327-338
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• 1995

The purpose of this study is to observe the effect of Nanganjeon on serum reaction in $CCl_4$ treated rats. In this study, the experimental rats divided four group(Control group, $CCl_4$ group, Haeganjeon group, and Sample group) : Under the same condition, control group were administered water, sample group were administered Nanganjeon for 7days. And then, both $CCl_4$ group and sample group were injected to abdomen with $CCl_4$ for 1days. The change of GOT, GPT, ALP, LDH, and bile acid activity in blood serum. The obtained results are summarised as follows : 1. In the change of SGOT, SGPT contents, as compared with control group, sample group was significantly decreased. 2. In the change of serum ALP contents, as compared with control group, sample group was significantly decreased. 3. In the change of serum bile acid contents, as compared with control group, sample group was significantly decreased. 4. In the change of serum LDH contents, as compared with control group, sample group was significantly decreased.

• 동국한의학연구소논문집
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• 제4권
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• pp.313-325
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• 1995

The purpose of this study is to observe the effect of Whaganjeon on serum reaction in $CCl_4$ treated rats. In this study, the experimental rats divided four group(Control group, $CCl_4$ group, Whaganjeon group, and Sample group) : Under the same condition, control group were administered water, sample group were administered Whaganjeon for 7days. And then, both $CCl_4$ group and sample group were injected to abdomen with $CCl_4$ for 1days. The change of GOT, GPT, ALP, LDH, and bile acid activity in blood serum. The obtained results are summarised as follows : 1. In the change of SGOT, SGPT contents, as compared with control group, sample group was significantly decreased. 2. In the change of serum ALP contents, as compared with control group, sample group was significantly decreased. 3. In the change of serum bile acid contents, as compared with control group, sample group was significantly decreased. 4. In the change of serum LDH contents, as compared with control group, sample group was significantly decreased.

• 동국한의학연구소논문집
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• 제5권
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• pp.53-77
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• 1996

The purpose of this study is to observe the effect of Sayeuksan on serum reaction in $CCl^4$ treated rats. In this study the experimental rats divided four group (Control group, $CCl^4$ group, sayeuksan group, and shiho, kamcho group) Under the same condition control group were administered water, sample group were administered Sayeuksan for 8days. and then, both $CCl_4$ group and sample group were injeccted to abdomen with $CCl_4$ for 1days. The change of GOT GPT ${\gamma}$-GTP ALP LDH activity in blood serum. The obtained results are summarised as follows : 1. In the change of GOT GPT contents, as compared with control group sample group was significantly decreased. 2. In the change of ${\gamma}$-GTP contents as compared with control group, sample group was significantly decreased. 3. In the change of ALP contents, as compared with control group, sample group was significantly decreased. 4. In the change of LDH contents, as compared with contol group, sample group was significantly decreased.

• 한국환경과학회지
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• 제21권10호
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• pp.1213-1219
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• 2012

The objective of this study was to investigate the effects of the feeding Cordyceps militaris extract on the improvement of the free fatty acid, lipid peroxide, creatinine and enzyme (creatine phosphokinase; CPK, lactate dehydrogenase; LDH, alkaline phosphatase; ALP, lecithin cholesterol acyltransferase; LCAT) activities in the sera of dietary hypercholesterolemic rats (SD strain, male) fed the experimental diets for 5 weeks. Concentrations of free fatty acid, lipid peroxide and CPK, LDH, ALP activities in sera were fairly reduced in the Cordyceps militaris extract administration group (CHE) than in the hypercholesterolemic diet group (CHD). However, no significance was found in the effect of an creatinine concentration among the groups. The LCAT activity in serum was increased in the Cordyceps militaris extract administration (CHE) than in the hypercholesterolemic diet group (CHD). From these results, Cordyceps militaris extracts were effective on the improvement of the lipid components and metabolic enzyme activities in sera of dietary hypercholesterolemic rats.

• 대한한방내과학회지
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• 제21권2호
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• pp.267-274
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• 2000

Objectives : In order to investigate the curative effect of Daesihotang-sosunggitang-gagambang on the liver injury of rats induced by $CCl_4$ and d-galactosamine. Methods : All animals were divided into 5 groups, those were normal group(untreated), control group(administrated with 0.9% Saline solution), sample I group(65mg/kg administrated), sample II group(130mg/kg administrated), positive control group(administrated with 200mg/kg silymarine). After the liver injury of rats induced by ccl4 and d-galactosamine, and cheked the serum transaminase(GOT, GPT) alkaline phosphatase(ALP), lactic dehydrogenase(LDH) for enzyme activities and triglyceride, total cholesterol amounts for serum component were measured. Result : The inhibitory effects on the serum GOT, GPT, LDH, ALP activities, serum total cholesterol content level in liver injury of rats induced by ccl4 were noted in both sample I group and sample II group. The inhibitory effects on the serum GPT, LDH activities and serum total cholesterol content level in liver injury of rats induced by d-galactosamine were noted in both sample I group and sample II group. The inhivitory effects on the serum GOT activities and triglyceride content level in liver injury of rats induced d-galactosamine were noted in sample I group, but it is not recognizable statistically. In sample II, they were noted. Conclusions : Deesihotang-sosoonggitang-gagambang has treatment effect against liver injury in rats induced by ccl4 and d-galactosamine. So it is required to study about the actions of mutual relation of medicines and path-mechanism by experiment.

• 대한한방내과학회지
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• 제22권3호
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• pp.309-320
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• 2001

Objectives : This study was done to investigate the protective effects of Samgiinjin-tang on liver injury of rats induced by CCI4 and d-galactosamine. Methods: All animals were divided into .5 groups, those were normal group(untreated), control group(treated with 0.9% Saline solution), sample I group(2,250mg/kg administrated), sample II group(4,500mg/kg administrated), Silymarin 200mg/kg administrated group. Liver injury of rats were induced by CCI4 and d-galactosamine, and then the serum transaminases(ALT&AST) alkaline phosphatase(ALP), lactic dehydrogenase(LDH) for enzyme activities, liver weight, lipid peroxidation and catalase, glutathione S-transferase(GST) for enzyme activities were measured. Results : The inhibitory effects on the serum ALT, AST activities in liver injury of rats induced by CCI4 were noted in both sample I and sample II group. The inhibitory effects on the serum ALP, LDH activities and the Lipid peroxidation of Mitochondria & Cytosol were noted in only sample II group. The decreased effects on the GST activities of Homogenate & Cytosol were inhibited in both sample I and sample II groups. The decreased effects on the GST activities of Mitochondria & Microsome were inhibited in sample II group. The inhibitory effects of the serum ALT, AST, LDH activities in liver injury of rats induced by d-galactosamine were noted in both sample I and sample II groups. In serum AST activities, sample II group. Conclusions : Samgiinjin-tang has protective effects against liver injury of rats induced by CCI4 and d-galactosamine. So it is required to study about the actions of mutual relation of medicines and patho-mechanism by experiment.

• 대한한방내과학회지
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• 제24권1호
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• pp.55-67
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• 2003

Objective : In order to investigate the curative effect of Jucha-whan on the protective liver of guinea pigs induced by $CCl_4$ and d-galactosamine, serum transaminase(GOT, GPT), lactic dehydrogenase(LDH), alkaline phosphatase(ALP), glutathione S-transferase(GST), superoxide dismutase(SOD) were used to measure enzyme activities and lipid peroxide level. Method : The subject animals were divided into 5 groups; a control group(untreated), a subject group(administered with 0.9% Saline solution), a sample I (500mg/kg administered), sample II group (1000mg/kg administered), positive control group(administered with 200mg/kg silymarine). Result : The inhibitory effects on the serum GOT, LDH, ALP, SOD and Lipid peroxide level activities in protective liver of mice induced by $CCl_4$ were noted both in the sample I group and sample II. The inhibitory effects on the serum GPT activities in protective liver of guinea pigs induced by $CCl_4$ were noted in sample II group, but it was not noted in the sample I. The inhibitory effects on the GST activities in protective liver of guinea pigs induced by $CCl_4$ were not noted in both sample I and sample group II. The inhibitory effects on the serum GOT, GPT activities in protective liver of guinea pigs induced by d-galactosamine were noted in both sample I and sample II, but it was not recognizable statistically. The inhibitory effects on the serum LDH activities in protective liver of guinea pigs induced by d-galactosamine were noted in sample II, but it was not noted in sample I group. Conclusion : According to the above results, it is considered that Jucha-whan has treatment effects on liver injury in guinea pigs induced by $CCl_4$ and d-galactosamine. So it is required to study about the actions of mutual relation of medicines and patho-mechanism through experiment.

• Applied Microscopy
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• 제40권1호
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• pp.29-35
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• 2010

본 연구는 galactosamine 투여로 인해 유발된 흰쥐의 간독성에 미치는 자초추출물의 효과를 알아보기 위해 자초 추출물을 각각 200 mg/kg과 500 mg/kg을 매일 1회씩 7일간 복강내 주사한 후, 마지막 자초 추출물 투여일에 galactosamine을 주사하여 다음과 같은 결론을 얻었다. AST, ALT, ALP, GGT와 LDH의 활성도에서는 LE200군이 GalN군에 비해 유의적으로 감소하였다. 그리고 조직학적 변화에서, GalN군은 염증세포의 침윤과 국소적인 괴사성 변화가 나타났지만, LE군들에서는 GalN 투여에 의한 변화가 상당히 경감되었다. 또한 미세구조적 변화에서는 GalN군은 미토콘드리아의 변형, 지방적의 증가, 조면소포체의 분절 등 세포소기관의 변형을 유발하였다. 그러나 LE군들에서는 대조군과 유사한 소견을 보여 주었다. 이상의 결과들로 보아, 자초추출물은 galactosamine 투여로 간독성을 유발시킨 흰쥐의 간 조직에 장애를 주지 않으면서 간독성을 완화시키는 효과가 있다고 사료된다.

• 한국응용과학기술학회지
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• 제36권2호
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• pp.383-393
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• 2019

Streptozotocin (STZ, 45 mg/kg body weight)으로 유도된 Sprague Dawley (SD)계 수컷 흰쥐에 5% 울금을 섭취시켰을 때, 혈청 크레아티닌(creatinine) 및 blood urea nitrogen (BUN)의 농도와 alkaline phosphatase (ALP), lactate dehydrogenase (LDH), aminotransferase (AST, ALT), 카탈라아제(catalase), 아밀라아제(amylase) 및 리파아제(lipase) 활성에 미치는 영향을 알아보기 위해 본 실험을 수행한 결과, 기본식이를 급여시킨 대조군(control)인 정상군은 BD군, 기본식이 실험군에 5% 울금 급여군(BT군), 당뇨 유발 실험군(BS군)인 질환 모델 대조군(control-diabetic)과 질환 실험군(diabetic, BS군)에 5% 울금을 급여시킨 실험군(ST군)으로 나누었다. 흰쥐의 혈청 크레아티닌 및 BUN 농도와 ALP, AST 및 ALT 활성은 유사한 경향으로 5% 울금의 급여가 감소시킨 것으로 확인되었다. SD계 수컷 흰쥐의 혈청 LDH 활성은 BS군에서 유의적인 차이를 나타내며 높은 활성을 보였으나(p<0.05), 5% 울금의 급여가 감소 효과를 나타내었다(p<0.05). 카탈라아제 활성은 BS군에서 유의적으로 낮은 활성을 나타내었고, ST군에서 활성을 증가시켰다(p<0.05). 혈청 아밀라아제 및 리파아제의 활성은 5% 울금의 급여가 감소시킨 것으로 확인되었다. 본 연구 결과, SD계 수컷 흰쥐를 통한 in vivo 실험에서 5% 울금의 섭취가 당뇨, 내인성 항산화 효소 및 간 기능 개선에 효과가 있는 것으로 나타났다.

• Environmental Analysis Health and Toxicology
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• 제7권1_2호
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• pp.35-43
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• 1992

Mugwort has been used as a Korean folk medicine in treating liver diseases acting as an analgesics, sedative, diuresis, choleretics. This study was perfomed to evaluate the effect of mugwort extracts on the changes of enzyme activities, lipid accumulation of the serum and liver, when hepatotoxicity was induced by benzo(a)pyrene. The results are as follows: 1. Mugwort water extract administration prevented the increase of serum and liver AST, ALT, LDH, ${\gamma}$-GTP, liver ALP activities and bilirubin content caused by B(a)P injection. 2. The increase of serum and liver ALT, LDH, ${\gamma}$-GTP, serum AST activities and liver bilirubin contents in B(a)P treated group were decreased by mugwort methanol extract treatment. 3. Serum and liver total cholesterol, phospholipid, triglyceride level and serum HDL-cholesterol level were increased by B(a)P treatment. After combined treatment of mugwort water and methanul extracts, these lipid content were significantly decreased. 4. The hepatotropic effect of mugwort water extract and after-treatment against B(a)P induced hepatotoxicity was superior to that of methanol extract and pretreatment.