• 제목/요약/키워드: ALP and LDH

검색결과 226건 처리시간 0.029초

기문(期門)에 대한 오공약침(蜈蚣藥鍼)이 D-Galactosamine으로 유발(誘發)된 간손상(肝損傷)에 미치는 영향(影響) (The effect of Scolopendrid Aqua-acupuncture applied to the L14 on Galactosamine-induced liver injury)

  • 최회강;김성철;윤대환;나창수;김성남;임정아;이성용;소기숙;조남근;황우준
    • Journal of Acupuncture Research
    • /
    • 제22권3호
    • /
    • pp.53-67
    • /
    • 2005
  • 기문혈(期門穴)에 대한(對) 오공자약침(蜈蚣雌藥鍼)이 D-Galactosamine에 의하여 유발된 간손상(肝損傷)에 미치는 영향을 실험적으로 규명하고자 흰쥐에게 약침을 처치한 후 혈청내의 G0T GPT, ${\gamma}$-GTP, Total bilirubin, LDH, ALP, Total cholesterol, Triglyceride 및 혈 액액내의 WBC, RBC, HGB, Hct 등을 관찰한 바 다음과 같은 결과를 얻었다. 1. GPT변화에서 대조군에 비하여 SHA-2군과 SHA-3군이 유의한 감소를 보였다. 2. Total bilirubin변화에서 대조군에 비하여 SHA-1군, SHA-2군과 SHA-3군이 유의한 감소를 보였다. 3. ${\gamma}$-GTP변화에서 대조군에 비하여 SHA-1군 과 SHA-2군이 유의한 감소를 보였다. 4. Total cholesterol변화에서 대조군에 비하여 SHA-2 군이 유의한 증가를 보였다. 5. Triglyceride변화에서 대조군에 비하여 SHA-1군, SHA-2군과 SHA-3군이 유의한 감소를 보였다. 6. HDL-cholesterol변화에서 대조군에 비하여 SHA-2군이 유의한 증가를 보였다. 7. 혈액내 변화에서 대조군에 비하여 WBC는 SHA-2군에서 유의한 감소를 보였으며, RBC는 SHA-2군과 SHA-3군에서 유의한 증가를 보였고, HGB는 SHA-2군과 SHA-3군에서 유의한 증가를 보였으며, Hct는 SHA-3군에서 유의한 증가를 보였다. 이상의 실험결과를 종합해보면 오공약침이 D-galactosamine으로 유발된 백서의 간손상에 효과적이라고 생각되며 간손상에 대한 예방, 치료적인 부분에서 향후 지속적인 연구가 필요하리라고 사료된다.

  • PDF

버터, 정어리유 및 홍화유의 혼합 급이가 흰쥐의 혈청 지질성분 및 간기능 효소 활성에 미치는 영향 (Effects of Feeding the Mixed Oils of Butter, Sardine and Safflower on the Lipid Components in Serum and Activities of Hepatic Functional Enzymes in Rats)

  • 김한수;정승용
    • 한국식품영양과학회지
    • /
    • 제21권6호
    • /
    • pp.608-616
    • /
    • 1992
  • n-3계 EPA와 DHA의 함유비율이 높은 정어리유와 n-6계 linoleic acid의 함유비율이 높은 홍화유의 혼합급여가 고지질 식이 흰쥐의 혈청 지질개선작용과 간기능 관련 효소의 활성에 미치는 영향을 구명하기 위해 Sprague Dawley계 숫 흰쥐에게 버터 식이를 대조군으로 하고 정어리유 및 홍화유의 혼합비율을 달리한 유지를 급여하여 4주간 실험 사육한 후 혈청의 지질성분 및 효소활성을 분석 검토한 결과는 다음과 같다. 혈청 aspartate aminotransferase 및 alanine aminotransferase, lactate dehydrogenase 및 alkaline phosphatase 활성은 대조군에 비해 전실험군에서 유의성있게 감소하였으며 특히 ALT, LDH, 활성은 5군 (4% 정어리유+4% 홍화유)에서 더욱 낮았다. 혈청 총콜레스테롤 및 HDL-콜레스테롤 농도는 대조군에 비해 전실험군에서 낮았으며 특히 5군에서 가장 낮았다. 혈청 LDL, LDL-콜레스테롤, 인지질 및 중성지질의 농도는 대조군에 비해 전실험군에서 낮았다. 혈청 유리콜레스테롤 및 콜레스테롤 에스테르 농도는 5군에서 가장 낮았으며, 에스테르 비는 대조군에서 가장 높았고, 2군(8% 올리브유)에서 가장 낮았다. 이상의 결과에서 혈청중 각종 지질성분의 농도 및 효소 활성 등으로 미루어 보아, n-3계 PUFA의 함유비율이 높은 정어리유와 n-6계 PUFA 함유비율이 높은 홍화유를 동량 혼합 급여하였을 때, 혈청의 지질개선 효과가 더욱 높은 것으로 나타났는데, 이는 시험유지의 P/S비 0.85, n-3P비가 2.85로서 적정한 혼합 비율로 인한 것이라 추정된다.

  • PDF

Hepatotoxicity and nephrotoxicity of saponin-enriched extract of Asparagus cochinchinensis in ICR mice

  • Sung, Ji Eun;Choi, Jun Young;Kim, Ji Eun;Lee, Hyun Ah;Yun, Woo Bin;Park, Jin Ju;Kim, Hye Ryeong;Song, Bo Ram;Kim, Dong Seob;Lee, Chung Yeoul;Lee, Hee Seob;Lim, Yong;Hwang, Dae Youn
    • Laboraroty Animal Research
    • /
    • 제33권2호
    • /
    • pp.57-67
    • /
    • 2017
  • The inhibitory effects of Asparagus cochinchinensis against inflammatory response induced by lipopolysaccharide (LPS), substance P and phthalic anhydride (PA) treatment were recently reported for some cell lines and animal models. To evaluate the hepatotoxicity and nephrotoxicity of A. cochinchinensis toward the livers and kidneys of ICR mice, alterations in related markers including body weight, organ weight, urine composition, liver pathology and kidney pathology were analyzed in male and female ICR mice after oral administration of 150, 300 and 600 mg/kg body weight/day saponin-enriched extract of A. cochinchinensis (SEAC) for 14 days. The saponin, total flavonoid and total phenol levels were found to be 57.2, 88.5 and 102.1 mg/g in SEAC, respectively, and the scavenging activity of SEAC gradually increased in a dose-dependent manner. Moreover, body and organ weight, clinical phenotypes, urine parameters and mice mortality did not differ between the vehicle and SEAC treated group. Furthermore, no significant alterations were measured in alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), blood urea nitrogen (BUN) and the serum creatinine (Cr) in the SEAC treated group relative to the vehicle treated group. Moreover, the specific pathological features induced by most toxic compounds were not observed upon liver and kidney histological analysis. Overall, the results of the present study suggest that SEAC does not induce any specific toxicity in the livers and kidneys of male and female ICR mice at doses of 600 mg/kg body weight/day.

인진호탕(茵蔯蒿湯) 추출액이 Diethylnitrosamine과 CCl4로 유발된 흰쥐의 간암(肝癌) 형성과 간경변(肝硬變)에 미치는 영향 (The Effect of Injinho-tang Extract on Hepatocellular Carcinogenesis and Hepatic Cirrhosis Induced by Diethylnitrosamine and CCl4 in Rats)

  • 이종범;허래경;성기문;문구;이종덕;원진희
    • 대한약침학회지
    • /
    • 제12권3호
    • /
    • pp.5-24
    • /
    • 2009
  • Injinho-tang(IJ) has been used for the treatment of hepatobiliary diseases. This study was performed to observe the effect of IJ extract on the hepatocellular carcinogenesis and hepatic cirrhosis induced by Diethylnitrosamine(DENA) and $CCl_4$ in Rats. Experimental groups were divided into two ; 8th and 12th week group, and subdivided into four; normal group(Nor), hepatocellular cancer and hepatic cirrhosis inducing control group(Con), and IJ extract 260mg/kg/day(IJA) or 520mg/kg/day(IJB) administered groups to Con. The results obtained are as follows: The body weight was decreased in the Con, IJA and IJB compared with the Nor from the 2nd week to the 12th week. The weight of liver and the weight of liver/100g body weight were decreased significantly in Con, IJA and IJB compared with the Nor. The activities of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) were significantly increased in the Con compared with Nor, but decreased in the IJA and IJB compared with Con from the 8th week group. The activities of alkaline phosphatase(ALP), lactacte dehydrogenase (LDH) and alpha fetoprotein(AFP) were increased significantly in the Con compared with Nor, but decreased in the IJA and IJB compared with Con. The activities of superoxide dismutase(SOD) were decreased in the IJA and IJB compared with Con, but the activities of catalase were increased in the IJA and IJB compared with Con. According to the light and electron microscopical observation, IJA and IJB improved the morphological and histopathological changes of the liver injured by DENA and $CCl_4$. The number of hepatic p53 positive cells was decreased in the IJA and IJB compared with Con. These results suggest that administration of IJ extract suppress or retard DENA and $CCl_4$-induced hepatocelluar carcinogenesis and hepatic cirrhosis in rats.

보중익기탕(補中益氣湯), 소음인(少陰人) 보중익기탕(補中益氣湯)과 그 구성약물군(構成藥物群)이 alloxan으로 유발(誘發)된 고혈당(高血糖) 백서(白鼠)에 미치는 영향(影響) (Effects of Bojungikgitang, Soeumin bojungikgitang and its component groups on diabetes in alloxan induced diabetic rats)

  • 박선동;고원도;신화석
    • 대한한의학방제학회지
    • /
    • 제9권1호
    • /
    • pp.215-230
    • /
    • 2001
  • The purpose of this study was to investigate the effect of Bojungikgitang(補中益氣湯), Soeumin bojungikgitang(少陰人 補中益氣湯) and its component groups on diabetes in alloxan induced diabetic rats. Sprague-Dawley male rats weighing $200{\pm}10\;gm$ were randomly assigned to one normal, one control and five experimental groups. The experimental groups was classified into to Bojungikgitang(B1 group), Soeumin bojungikgitang(B2 group), Bogihyulyak(B0 group), Siho-sengma(B1+ group) and Gwakhyang- soyeup(B2+ group). Diabetes was experimentally induced by abdomenal injection of 100 mg/kg of body weight of alloxan. Animals were sacrificed at the 6th day and 11th day of diabetic states. The results were obtained as follows: In carbohydrate metabolism, the level of glucose in control group was three-fold higher after alloxan injection than that of normal group. In B1, B2 groups the level of glucose was decreased by 39% and 43% compared with that of control group, and in component groups it was decreased by 73%, 81%, and 80% respectively. In fat metabolism, the level of triglyceride and total cholesterol in serum were lower in B1 and B2 groups with high significant difference and had similar tendency in components groups compared with that of control group. The level of HDL cholesterol on serum were increased in B1 and B2 group with significant difference. And in component group it had similar tendency compared with that of control group. In protein metabolism, the level of total protein and albumin were higher in B1 and B2 group with significant difference, and in component group also increased but with no significancy compared with control group. In kidney function, the level of creatinine group with significant difference, and other compared with control group. In liver function, the level of GOT, GPT, ${\gamma}$-GTP. ALP and bilirubin were lower in B1 and B2 group with significant difference, and in component had similar tendency compared with control group. But in the level of LDH, it was decreased with no significancy. These results indicate that Bojungikgitang(補中益氣湯), Soeumin bojungikgitang(少陰人 補中益氣湯) has strong effect on diabetes and it is useful to prevent diabetes, and in component groups the difference between B1+ and B2+ group was not significant.

  • PDF

Prognostic Factor Analysis of Overall Survival in Gastric Cancer from Two Phase III Studies of Second-line Ramucirumab (REGARD and RAINBOW) Using Pooled Patient Data

  • Fuchs, Charles S.;Muro, Kei;Tomasek, Jiri;Van Cutsem, Eric;Cho, Jae Yong;Oh, Sang-Cheul;Safran, Howard;Bodoky, Gyorgy;Chau, Ian;Shimada, Yasuhiro;Al-Batran, Salah-Eddin;Passalacqua, Rodolfo;Ohtsu, Atsushi;Emig, Michael;Ferry, David;Chandrawansa, Kumari;Hsu, Yanzhi;Sashegyi, Andreas;Liepa, Astra M.;Wilke, Hansjochen
    • Journal of Gastric Cancer
    • /
    • 제17권2호
    • /
    • pp.132-144
    • /
    • 2017
  • Purpose: To identify baseline prognostic factors for survival in patients with disease progression, during or after chemotherapy for the treatment of advanced gastric or gastroesophageal junction (GEJ) cancer. Materials and Methods: We pooled data from patients randomized between 2009 and 2012 in 2 phase III, global double-blind studies of ramucirumab for the treatment of advanced gastric or GEJ adenocarcinoma following disease progression on first-line platinum- and/or fluoropyrimidine-containing therapy (REGARD and RAINBOW). Forty-one key baseline clinical and laboratory factors common in both studies were examined. Model building started with covariate screening using univariate Cox models (significance level=0.05). A stepwise multivariable Cox model identified the final prognostic factors (entry+exit significance level=0.01). Cox models were stratified by treatment and geographic region. The process was repeated to identify baseline prognostic quality of life (QoL) parameters. Results: Of 1,020 randomized patients, 953 (93%) patients without any missing covariates were included in the analysis. We identified 12 independent prognostic factors of poor survival: 1) peritoneal metastases; 2) Eastern Cooperative Oncology Group (ECOG) performance score 1; 3) the presence of a primary tumor; 4) time to progression since prior therapy <6 months; 5) poor/unknown tumor differentiation; abnormally low blood levels of 6) albumin, 7) sodium, and/or 8) lymphocytes; and abnormally high blood levels of 9) neutrophils, 10) aspartate aminotransferase (AST), 11) alkaline phosphatase (ALP), and/or 12) lactate dehydrogenase (LDH). Factors were used to devise a 4-tier prognostic index (median overall survival [OS] by risk [months]: high=3.4, moderate=6.4, medium=9.9, and low=14.5; Harrell's C-index=0.66; 95% confidence interval [CI], 0.64-0.68). Addition of QoL to the model identified patient-reported appetite loss as an independent prognostic factor. Conclusions: The identified prognostic factors and the reported prognostic index may help clinical decision-making, patient stratification, and planning of future clinical studies.

Influence of Dietary Supplementation of Condensed Tannins through Leaf Meal Mixture on Intake, Nutrient Utilization and Performance of Haemonchus contortus Infected Sheep

  • Pathak, A.K.;Dutta, Narayan;Banerjee, P.S.;Pattanaik, A.K.;Sharma, K.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제26권10호
    • /
    • pp.1446-1458
    • /
    • 2013
  • The study assessed the effect of dietary supplementation of leaf meal mixture (LMM) containing condensed tannins (CT) on feed intake, nutrient utilization and performance of sheep infected with Haemonchus contortus. Eighteen adult sheep of similar age and body weight ($25.03{\pm}1.52$) were included in this study and out of these, 12 sheep were infected with single dose of infective third stage larvae of H. contortus at 2,000 larvae per sheep. The experimental sheep were allocated in three different groups' i.e. negative control (NC; no infection), control (C; H. contortus infected) and treatment (T; H. contortus infected+CT at 1.5% of the DM through LMM) and the experiment was conducted for a period of 90 d. The intake of dry matter (DM), organic matter (OM) and digestibility of DM, OM, neutral detergent fibre (NDF) and acid detergent fibre (ADF) were comparable among three animal groups. However, digestibility of crude protein (CP) and ether extract (EE) were significantly (p<0.05) higher in NC group as compared to both C and T groups. Nitrogen (N) retention (g/d or % of N intake) was significantly (p = 0.038) lower in C group as compared to T and NC groups. Daily intake (g/kg $W^{0.75}$) of digestible crude protein (DCP), digestible organic matter (DOM) and total digestible nutrient (TDN) did not differ significantly (p<0.05) in the three groups. Haemoglobin (Hb) and packed cell volume (PCV) were significantly (p<0.001) higher in treatment group as compared to control. The level of Hb and PCV reduced (p<0.001) after 30 days of experimental feeding. CT significantly (p<0.001) reduced serum urea in T group as compared to NC and C groups. Serum proteins differed significantly (p<0.01) among the three groups. The activity of serum enzymes AST, ALT, ALP and LDH were also statistically non significant (p<0.05) among treatments. The weight of abomasal lymph nodes (ALN) in T group was higher (p<0.05) than in C group. Treatment group had lower (p<0.05) total worms and fecal egg count compared to control group. It may be concluded that dietary supplementation of CT through LMM significantly improved the N retention, and inhibited the different developmental stages of Haemonchus contortus in experimental sheep.

Antihepatotoxic effect of Heat-treated Allium victorialis var. platyphyllum in $CCl_4-induced$ Rats and the Gas Chromatographic Analysis of Volatile Sulfur Substances

  • Park, Hee-Juhn;Jung, Hyun-Ju;Lim, Sang-Cheol;Jung, Won-Tae;Kim, Won-Bae;Park, Kwang-Kyun;Lee, Jin-Ha;Choi, Jong-Won
    • Natural Product Sciences
    • /
    • 제11권2호
    • /
    • pp.67-74
    • /
    • 2005
  • The ethanolic extracts of the leaves and bulbs of Allium victorialis var. platyphyllum (Liliaceae) collected from Daegwallyoung (D) and Ullung Island (U) in Korea were obtained using three different extracting methods. The first extracts, DL-1 DB-1, UL-1 and UB-1, were obtained from leaves (L) and bulbs (B) dried at $90^{\circ}C$, respectively, and the second extracts, DL-2, DB-2, UL-2 and UB-2, were obtained by extracting the leaves and bulbs of fresh plant parts. The third extracts DL-3, DB-3, UL-3 and UB-3 were obtained by incubating leaves and bulbs at $36^{\circ}C$. The six extracts obtained from A. victorialis var. platyphyllum at Daegwanllyoung (cultivated site) were orally administered to examine for a possible antihepatotoxic effect in $CCl_4-induced$ rats. DL-1 exhibited the most pronounced effect. The extracts inhibited serum ALT, AST, SDH, ${\gamma}-GT$, ALP and LDH activities elevated by $CCl_4$ injection and attenuated decreased glutathione S-transferase, glutatione reductase and ${\gamma}-glutamylcysteine$ synthetase activities and a decreased hepatic glutathione. However, the extracts obtained from Ullung Is. (native site) were less active than the extracts from Daegwallyoung, suggesting that A. victorialis var. platyphyllum from the cultivated site is more useful for functional food than of native site. These results also suggest that the antihepatotoxic effect is due to a higher content of hepatic glutathione. Gas chromatography of the twelve extracts showed significantly different sulfides, disulfides or trisulfides contents belonging to volatile sulfur substances (VSS). Nine components were identified on the basis of their mass spectra, namely, dimethyl disulfide, dimethyl trisulfide, diallyl disulfide, dipropyl disulfide, allyl methyl sulfide, allyl methyl trisulfide, 2-vinyl-4H-1,3-dithiin, 3,4-dihydro-3-vinyl-1,2-dithiin, and allithiamine. Extract DL-1 had the highest VSS content. Dried plant materials contained larger amounts of the VSSs than other extracts, and the leaves contained larger amount than the bulbs. These results suggest that heat treatment increases the antiheaptotoxic ability of A. victorialis var. platyphyllum by increasing the proportion of VSSs.

왕부류행(王不留行)과 경엽여루채(硬葉女婁菜)가 알콜 투여로 유발된 흰쥐의 고지혈증과 간 손상의 예방에 미치는 영향 (Preventive effects of Saponariae Vaccariae Semen and Melandryi Firmi Herba on hyperlipidemia and liver damage induced by alcohol)

  • 최재영;서부일;오달석;박지하;노성수
    • 대한본초학회지
    • /
    • 제27권1호
    • /
    • pp.27-34
    • /
    • 2012
  • Objectives : This study was performed to compare the preventive effect of water extracts from Saponariae Vaccariae Semen(SVS) and Melandryi Firmi Herba(MFH) on hyperlipidemia and liver damage induced by alcohol. Methods : Except for the normal group, I fed rat on 25% alcohol for 55 days. And each extract was administrated for the same period. I measured the serum component in rat's blood, body weight and weight of liver. Results : At first, I observed effects of SVS and MFH on hyperlipidemia induced by alcohol. SVS and MFH group didn't show the significant decrease of total cholesterol in comparison with those of the control group. SVS and MFH group showed the significant increase of HDL-cholesterol in comparison with those of the control group. SVS group showed the significant decrease of triglyceride in comparison with those of the control group, but MFH group didn't show the significant decrease of triglyceride in comparison with those of the control group. SVS group showed the significant increase of body weight in comparison with those of the control group at 4weeks and 8weeks. But, MFH group didn't show the significant changes of body weight in comparison with those of the control group at 4weeks and 8weeks. By the way, SVS group showed the significant decrease of triglyceride in comparison with those of MFH group. SVS group showed the significant increase of body weight in comparison with those of MFH group at 8weeks. At second, I observed effects of SVS and MFH on liver damage induced by alcohol. SVS and MFH group showed the significant decrease of AST(aspartate aminotransferase), ALT(alanine aminotransferase), ALP(alkaline phosphatase) and LDH(lactate dehydrogenase) in comparison with those of the control group. SVS group showed the significant increase of liver weight in comparison with those of the control group, but MFH group didn't show the significant increase of liver weight in comparison with those of the control group. By the way, SVS group showed the significant increase of liver weight in comparison with those of MFH group. Conclusions : Reviewing these experimental results, it suggests that water extracts from SVS and MFH have pharmaceutical preventive efficacy on hyperlipidemia and liver damage induced by alcohol. Therefore further additional study should be conducted to elucidate the pharmaceutical efficacy of these in depth.

오적산가미방(五積散加味方) 추출물이 $CCl_4$ 중독 Rat의 간기능 회복에 미치는 영향 (Effects of Ojeoksangamibang Extract on the Recovery of Liver Function in $CCl_4$-exposed Rats)

  • 이수연;박원형;차윤엽;이은
    • 한방재활의학과학회지
    • /
    • 제23권3호
    • /
    • pp.45-53
    • /
    • 2013
  • Objectives The objective of present study was to investigate the effect of Ojeoksangamibang ($W\check{u}j\bar{i}s\check{a}nji\bar{a}w\grave{e}if\bar{a}ng$) extract on recovery of liver function in carbontetrachloride ($CCl_4$)-exposed rat. Methods Male rats weighing $230{\pm}7.21g$ fed experimental diet for 1 week and 28 rats were divided into 4 groups. Each of 7 rats was devided into a control group and experimental groups. We fed a control group of rats a basal diet and administered normal saline (100 mg/kg, 1 time/1 day) for 3 weeks. And we fed each experimental group of rats basal diet and administered an extract of Ojeoksangamibang extracts (100 mg/kg, 200 mg/kg, 300 mg/kg, 1 time/1 day) for 3 weeks. We measured lipid of plasma and liver, concentration of proinflammatory cytokines ($IL-1{\beta}$, IL-6 and IL-10). Statistical analysis was done by one-way analysis of variance (ANOVA) and Duncan's multiple range test with significance level at p<0.05. Results Plasma a-fetoprotein (AFP) and total protein concentration showed a tendency to decrease in Ojeoksangamibang extract-treated groups. However, plasma albumin concentration showed no significant differences in all treatment groups. Activity of plasma Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) in the Ojeoksangamibang extract-treated groups, increased addition amount of Ojeoksangamibang extracts tended to decline. Alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and ${\gamma}$-glutamyl transferase (${\gamma}$-GT) activities showed a tendency to decrease in Ojeoksangamibang extract-treated groups, increased addition amount of Ojeoksangamibang extracts tended to decline. Concentration of plasma triglyceride and total cholesterol showed a lower value than that of control group. The liver $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ concentration were decreased, and IL-10 was increased in Ojeoksangamibang extract groups, compared to control group. Plasma $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ concentration were decreased, and IL-10 was increased in Ojeoksangamibang extract groups, compared to control group. Conclusions This study suggested that Ojeoksangamibang may alleviate liver inflammatory reaction induced by liver toxicity.