• 한국미생물·생명공학회지
• /
• 제44권3호
• /
• pp.341-348
• /
• 2016

본 연구는 폐글리세롤을 탄소원으로 사용하여 생장저해 없이 아미노레블린산(ALA)을 생산하는 미생물 공정을 개발하는데 그 목적이 있다. 폐글리세롤이 첨가된 배지에서 ALA 생산균주(E. coli/pH-hemA)를 배양하여 얻은 세포밀도와 ALA 생산량은 순수 글리세롤이 첨가된 배지에서 얻은 값보다 각각 1.8배와 1.2배 낮았다. 트레할로스를 첨가(30 g/l 또는 100 g/l) 함에 따라 세포생장과 ALA 생산성이 향상되었다. otsBA를 공동 발현하는 엔지니어링 균주(E. coli/pH-hemA/pS-otsBA)를 제작하여 순수 또는 폐글리세롤이 첨가된 배지에서 그 성능을 평가하였다. 폐글리세롤이 함유된 배지에서 엔지니어링 균주의 세포생장 및 생산성을 향상시키기 위하여 IPTG의 최적 첨가농도 및 시기를 결정하였다. 지수생장 초기에 0.6 mM의 IPTG를 첨가한 배양에서 세포 생장과 ALA 생산량이 최대치를 나타내었다. 이때 얻은 OD₆₀₀(세포 밀도)와 ALA 농도는 각각 16과 2,121 mg/l였는데, 이는 순수 글리세롤이 첨가된 배지에서 얻은 생산량과 비슷한 값이다. 본 결과는 ALA 생산균주에서 트레할로스 생합성 오페론의 최적 공동 발현을 통해 폐글리세롤에 대한 저항성을 높임으로써 추가적인 전처리 없이 폐글리세롤을 탄소원으로 직접 사용할 수 있음을 보여준다.

• Nutrition Research and Practice
• /
• 제12권2호
• /
• pp.93-100
• /
• 2018

BACKGROUND/OBJECTIVE: Oxidative stress plays a key role in neuronal cell damage, which is associated with neurodegenerative disease. The aim of present study was to investigate the neuroprotective effects of perilla oil (PO) and its active component, alpha-linolenic acid (ALA), against hydrogen peroxide $(H_2O_2)$-induced oxidative stress in SH-SY5Y neuronal cells. MATERIALS/METHODS: The SH-SY5Y human neuroblastoma cells exposed to $250{\mu}M$ $H_2O_2$ for 24 h were treated with different concentrations of PO (25, 125, 250 and $500{\mu}g/mL$) and its major fatty acid, ALA (1, 2.5, 5 and $25{\mu}g/mL$). We examined the effects of PO and ALA on $H_2O_2$-induced cell viability, lactate dehydrogenase (LDH) release, and nuclear condensation. Moreover, we determined whether PO and ALA regulated the apoptosis-related protein expressions, such as cleaved-poly ADP ribose polymerase (PARP), cleaved caspase-9 and -3, BCL-2 and BAX. RESULTS: Treatment of $H_2O_2$ resulted in decreased cell viability, increased LDH release, and increase in the nuclei condensation as indicated by Hoechst 33342 staining. However, PO and ALA treatment significantly attenuated the neuronal cell death, indicating that PO and ALA potently blocked the $H_2O_2$-induced neuronal apoptosis. Furthermore, cleaved-PARP, cleaved caspase-9 and -3 activations were significantly decreased in the presence of PO and ALA, and the $H_2O_2$-induced up-regulated BAX/BCL-2 ratio was blocked after treatment with PO and ALA. CONCLUSIONS: PO and its main fatty acid, ALA, exerted the protective activity from neuronal oxidative stress induced by $H_2O_2$. They regulated apoptotic pathway in neuronal cell death by alleviation of BAX/BCL-2 ratio, and down-regulation of cleaved-PARP and cleaved caspase-9 and -3. Although further studies are required to verify the protective mechanisms of PO and ALA from neuronal damage, PO and ALA are the promising agent against oxidative stress-induced apoptotic neuronal cell death.

• 경영정보학연구
• /
• 제21권2호
• /
• pp.117-137
• /
• 2019

빅데이터 시대를 맞아 다양한 도메인에서 수없이 많은 데이터들이 생산되면서 데이터 사이언스가 대중화 되었고, 데이터의 힘이 곧 경쟁력인 시대가 되었다. 특히 전 세계 데이터의 80% 이상을 차지하는 비정형 데이터에 대한 관심이 부각되고 있다. 소셜 미디어의 발전과 더불어 비정형 데이터의 대부분은 텍스트 데이터의 형태로 발생하고 있으며, 마케팅, 금융, 유통 등 다양한 분야에서 중요한 역할을 하고 있다. 하지만 이러한 소셜 미디어를 활용한 텍스트 마이닝은 수치형 데이터를 활용한 데이터 마이닝 분야에 비해 접근이 어렵고 복잡해 기대에 비해 그 활용도가 높지 못한 실정이다. 이에 본 연구는 프로그래밍 언어나 고사양 하드웨어나 솔루션에 의존하지 않고, 쉽고 간편한 소셜 미디어 텍스트 마이닝을 위한 통합 애플리케이션으로 Korean Natural Language Application(KoALA)을 개발하고자 한다. KoALA는 소셜 미디어 텍스트 마이닝에 특화된 애플리케이션으로, 한글, 영문을 가리지 않고 분석 가능한 통합 애플리케이션이다. 데이터 수집에서 전처리, 분석, 그리고 시각화에 이르는 전 과정을 처리해준다. 본 논문에서는 디자인 사이언스(design science) 방법론을 활용해 KoALA 애플리케이션을 디자인, 구현, 적용하는 과정에 대해서 다룬다. 마지막으로 블록체인 비즈니스 관련 사례를 들어 KoALA의 실제 활용방안에 대해서 다룬다. 본 논문을 통해 소셜 미디어 텍스트 마이닝의 대중화와 다양한 도메인에서 텍스트 마이닝의 실무적, 학술적 활용을 기대해 본다.

• 대한화학회지
• /
• 제42권2호
• /
• pp.214-219
• /
• 1998

Dynorphin B유도체들인 $[Arg^{11},\;D-Ala^{12}]$ dynorphin B, $[D-Ala^2,\;Ala^6,\;Arg^{11},\;D-Ala^{12}]$ dynorphin B 및 dynorphin B (1-11)를 고상법으로 합성하였다.에탄올 용액에서 2%의 디비닐벤젠으로 교차결합된 파라-염화메틸폴리스티렌 수지와 Thr을 반응시켜 수지 1g당 1.20 mmol의 Thr을 치환시켰다. 모든 아미노산의 아미노기는 t-Boc기로 보호하였으며 Tyr과Arg의 곁사슬은 2,6-디클로로벤질기와 니트로기로 각각 보호하였다. 각 유도체는 DCC와HOBT를 짝지음 시약으로 사용하여 단계적 합성법으로 합성하였으며 생성물은 MeOH/MeCN (3/1)을 전개용매로 하여 Sephadex LH-20 column (2${\times}$50 cm)으로 정제하였으며 HPLC와 아미노산 분석기로 확인하였다.

• 대한치과보철학회지
• /
• 제14권1호
• /
• pp.35-40
• /
• 1976

The author observed the positional relationship of maxillary cuspid to ala-lacrimal duct line in the 594 Koreans(329 male, 265 female) who had relatively normal teeth arrangement, aged 16 to 70. The relationship of ala-lacrimal duct line to the distal angie of maxilly cuspid of the subjects were classified as follows; Type A : Ala-lacrimal duct line was lotated more medially than the distal angle of maxillary cuspid. Type B : Ala-lacrimal duct line coincided with the distal angle of maxillary cuspid. Type C: Ala-lacrimal duct line was located more distally than the distal angle of maxillary cuspid. The occlusal relationship of the maxillary first molar to the mandibular first molar of the subjects were classified as follows; Group I : Group of neutrocclusion relationship. Group II : Group of distocclusion relationship. Group III : Group of mesiocclusion relationship. The obtained results were as follows; 1) The positional relationship of maxillary cuspid to ala-lacrimal duct line were as follows; (Male) Type A: $68.5%{\pm}1.58m%$, Type B: $22.5%{\pm}2.30m%$, Type C: $9.1%{\pm}\1.58m%$ (Female) Type A : $64.5%{\pm}2.94m%$, Type B: $26.5%{\pm}2.71m%$, TypeC: $8.9%{\pm}1.73m%$ 2) Ala-lacrimal duct line located more distally in group II and group III than group I at the point of the positional relationship of upper and lower first molar. 3) Average distance and standard deviation in type A were as follows; (Male) Right: $2.17mm.{\pm}1.01mm.$ Left: $2.27mm.{\pm}1.02mm.$ (Female) Right: $2.06mm.{\pm}0.70mm.$ Left: $2.24mm.{\pm}0.80mm.$ 4) Average distance and standard deviation in type C were as follows; (Male), Right: $1.47mm.{\pm}0.85mm.$ Left: $1.52mm.{\pm}0.97mm.$ (Female) Right: $1.04mm.{\pm}0.44mm.$ Left: $1.26mm.{\pm}0.89mm.$ The results of 3) and 4) had no significant difference between the male and female.

• Reproductive and Developmental Biology
• /
• 제40권4호
• /
• pp.33-37
• /
• 2016

This study was conducted to evaluate effect of ${\alpha}$-linolenic acid (ALA) and bovine serum albumin (BSA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-hand method and cryopreserved using freezing extender containing 3 ng/mL ALA and/or $20\;{\mu}g/mL$ BSA. Cryo-preserved boar sperms were thawed in $37^{\circ}C$ water-bath for 45 sec to analysis. Viability, acrosome reaction, and mitochondrial intact were analyzed using flow cytometry. In results, viability of frozen-thawed boar sperm was significantly higher in only ALA+BSA supplement group than control group (p<0.05), whereas there was no difference either in ALA or BSA supplement. However, acrosome reacted sperm in both of live and all sperm population were significantly decreased in all treatment groups than control (p<0.05). Interestingly, mitochondrial intact of boar sperm was enhanced in ALA and ALA+BSA groups compared with control (p<0.05). In this study, we showed that supplementation of ALA and BSA in freezing extender enhanced the sperm viability, mitochondrial intact and decrease acrosomal membrane damage. In conclusion, our findings suggest that quality of frozen-thawed sperm in mammalians could improve by using of ALA and BSA.

• International Journal of Oral Biology
• /
• 제46권1호
• /
• pp.15-22
• /
• 2021

Alpha-lipoic acid (ALA) is a naturally occurring antioxidant and has been previously used to treat diabetes and cardiovascular disease. However, the autophagy effects of ALA against oxidative stress-induced dopaminergic neuronal cell injury remain unclear. The aim of this study was to investigate the role of ALA in autophagy and apoptosis against oxidative stress in the SH-SY5Y human dopaminergic neuronal cell line. We examined SH-SY5Y phenotypes using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay (cell viability/proliferation), 4′,6-diamidino-2-phenylindole dihydrochloride nuclear staining, Live/Dead cell assay, cellular reactive oxygen species (ROS) assay, immunoblotting, and immunocytochemistry. Our data showed ALA attenuated hydrogen peroxide (H2O2)-induced ROS generation and cell death. ALA effectively suppressed Bax up-regulation and Bcl-2 and Bcl-xL down-regulation. Furthermore, ALA increased the expression of the antioxidant enzyme, heme oxygenase-1. Moreover, the expression of Beclin-1 and LC-3 autophagy biomarkers was decreased by ALA in our cell model. Combined, these data suggest ALA protects human dopaminergic neuronal cells against H2O2-induced cell injury by inhibiting autophagy and apoptosis.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XII)
• /
• pp.87-90
• /
• 2003

ALA is an essential intermediate in the tetrapyrrole biosynthesis pathway and has applications as a biodegradable herbicide and insecticide as well as medical applications including photodynamic therapy of cancers. For the development of mass production process of ALA it is necessary to on-line monitor some metabolites such as glycine, succinate, LA and ALA. In this work, medium compositions, pH conditions, induction and addition of LA were investigated to increase the production of ALA by recombinant E. coli. A 2-dimensional fluorescence sensor was used to monitor the processe and the fluorescence spectra were correlated with on- and off-line data like ALAS, ALAD, cell mass etc.

• Bulletin of the Korean Chemical Society
• /
• 제35권4호
• /
• pp.1087-1090
• /
• 2014

A simple and general method that selectively introduces metal binding sites into a protein can greatly increase the ability to design and biosynthesize artificial metalloproteins. Here, we report the incorporation of a phenanthroline-containing amino acid (Phen-Ala) into proteins in Escherichia coli by using the $tRNA{^{Tyr}}_{CUA}$ and tyrosyl aminoacyl-tRNA synthetase pair (BpyRS) from Methanococcus jannaschii, which was originally developed for a bipyridine-containing amino acid (Bpy-Ala). The incorporation efficiency of BpyRS for Phen-Ala was comparable to that for Bpy-Ala. Because of its high metal-binding ability and characteristic spectral properties, Phen-Ala can be a useful alternative to the existing metal-chelating amino acids for the design and synthesis of artificial metalloproteins.

• BMB Reports
• /
• 제43권5호
• /
• pp.330-336
• /
• 2010

5-Aminolevulinate (ALA) is well-known as an essential biosynthetic precursor of all tetrapyrrole compounds, which has been suggested to improve plant salt tolerance by exogenous application. In this work, the gene encoding aminolevulinate synthase (ALA-S) in yeast (Saccharomyces cerevisiae Hem1) was introduced into the genome of Arabidopsis controlled by the Arabidopsis thaliana HemA1 gene promoter. All transgenic lines were able to transcribe the YHem1 gene, especially under light condition. The chimeric protein (YHem1-EGFP) was found co-localizing with the mitochondria in onion epidermal cells. The transgenic Arabidopsis plants could synthesize more endogenous ALA with higher levels of metabolites including chlorophyll and heme. When the $T_2$ homozygous seeds were cultured under NaCl stress, their germination and seedling growth were much better than the wild type. Therefore, introduction of ALA-S gene led to higher level of ALA metabolism with more salt tolerance in higher plants.