• 대한본초학회지
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• 제26권4호
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• pp.49-57
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• 2011

Objective : Allergic asthma is a chronic airway disease that affects millions of people in the developed world. The disease is characterized by concurring airway inflammation, Th2 cytokine production, increased mucus secretion, airway hyperresponsiveness (AHR) to inhaled antigen, and pulmonary fibrosis. To investigate the therapeutic and anti-asthmatic effects of Drynariae Rhizoma (DR), we examined the influence of DR on the development of pulmonary eosinophilic inflammation and airway hyperresponsiveness in a mouse model of allergic asthma. Methods : In this study, BALB/c mice were systemically sensitized to ovalbumin (OVA) followed intratracheally, intraperitoneally, and by aerosol allergen challenges. We investigated the effect of DR on airway hyperresponsiveness, pulmonary eosinophilic infiltration, various immune cell phenotypes, Th2 cytokine production and OVA specific IgE production in a mouse model of asthma. Results : In asthmatic mice, we found that DR.treated groups had suppressed eosinophil infiltration, allergic airway inflammation and AHR by suppressing the production of IL-5, IL-13 and OVA specific IgE. Conclusions : Our data suggest that the therapeutic mechanism by which DR effectively treats asthma is based on reductions of Th2 cytokines (IL-5), eotaxin, OVA-specific IgE production and eosinophil infiltration.

• 대한의생명과학회지
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• 제16권2호
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• pp.83-90
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• 2010

Hesperidin, a member of the flavanone group of flavonoids, can be isolated in large amounts from the rinds of some citrus species [e.g., Citrus aurantium L. (bitter orange), Citrus sinensis L. (sweet orange) and Citrus unshiu Marcov. (satsuma mandarin)], and has been reported to have anticarcinogenic, antihypotensive and antimicrobial properties. Despite the efficacy of these polyphenolic compounds as immune modulators, the effects of the flavonoids are poorly understood about allergic effect. In this study, we investigated whether hesperidin could influence on Th1 and Th2 balance. Allergic reactions included an increase in the number of eosinophils in bronchoalveolar lavage (BAL) fluid, an increase in inflammatory cell infiltration into the lung tissue around blood vessels and airways, airway luminal narrowing, the development of airway hyper-responsiveness (AHR). The administration of hesperidin before the last airway OVA challenge resulted in a significant inhibition of all asthmatic reactions. Accordingly, this study may provide evidence that hesperidin plays a critical role in the amelioration of the pathogenetic process of asthma in mice. These findings provide new insight into the immunopharmacological role of hesperidin in terms of its effects in a murine model of asthma, and also broaden current perspectives in our understanding of the immunopharmacological functions of hesperidin.

• 한방안이비인후피부과학회지
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• 제20권1호통권32호
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• pp.177-194
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• 2007

Fructus of Rubus coreanus $M_{IQ.}$. (FRCM) has been used for stuttering urination, prostate gland disease, and impotence in Korean traditional medicine. Water extract of FRCM (WFRCM) treatment significantly attenuated airway hyperreactivity (AHR). Airway recruitment of leukocytes and eosinophils was also markedly reduced by WFRCM administration, suggesting that WFRCM can alleviate the airway inflammation. However, the level of cytokine (IL-4, IL-5, and IL-13) in bronchoalveolar lavage fluid (BALF) and lung was not different compared with positive control. WFRCM reduced the number of draining lymph node cells during OVA-induced allergic asthma. I further examined transcription level of cytokine in lung. WFRCM treatment reduced IL-4 and IL-13 mRNA in lung and inhibited IgE and IgG1 but not IgG2a. My data suggest that WFRCM attenuates OVA-induced allergic asthma through inhibition of Th2 cell response.

• 한방안이비인후피부과학회지
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• 제19권2호
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• pp.1-18
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• 2006

Objective : Although management of asthma has become increasingly effective, its cure remains elusive, necessitating a new modality to prevent or eliminate causes triggering clinical progress. Based in the clinical experiences, a novel decoration Cheongsangbiyeum (CSB), has been developed to treat asthma, which consists of Polyporus, Semen Myristicae, Pericarpium citri Reticulatae, Rhizoma Cimicifugae, Cortex Albizziae, Fructus Rubi, Rhizoma Zedoariae, and Rhizoma Rhei. In the current study, its anti-asthmatic efficacy was evaluated using a mouse model of asthma. Methods : Experimental allergic asthma was induced by repeated intraperitioneal sensitization and intranasal challenge of ovalbumin (OVA). Water extract of CSB (1 mg/mouse/day) was administrated orally whereas control mice on given with identical volume of phosphate-buffered saline (PBS) for 5 days during the course of antigen challenge. When airway hyperreactivity(AHR) measured by ${\bata}-methacoline-induced$ airflow obstruction was compared, AHR of CSB-treated mice was significantly lower than those of control mice, indicating that CM extract can attenuate an asthmatic symptom. Airway recruitment of leukocytes and eosinophils was also markedly reduced by CSB treatment suggesting that oral treatment of CSB can alleviate the airway inflammation. For a better understanding of possible mechanisms underlying anti-asthmatic effet of CSB, cytokine (IL-4, IL-5, IL-13 and $IFN{\gamma}$ levels in bronchoalveola lavage fluid (BALF) and lung tissues were determined. Results : The results showed that cytokine levels were significantly lowered by CSB treatment. Additionally, number of draining lymph node cells was significantly lower than those of control mice. These data indicate that CSB suppress in vivo allergen-specific response. However, notably, levels of type 2 cytokines such as IL-5 and IL-13 were more profoundly influenced. Moreover, in vitro OVA-specific proliferative response and type 2 cytokine (IL-4, IL-5 and IL-13) production lymph node cells was markedly decreased in CSB-treated mice, whereas their $IFN{\gamma}$ production was not significantly altered Thrse data clearly showed a preferential inhibition of type 2 T cell (Th2) response by CSB treatment. This finding was also supported by serum antibody data showing that levels of OVA-specific type 2 antibodies, IgE and IgG1, in CSB-treated mice were significantly lower than in control mice, while type 1 antibody, IgG2a level m rather higher than controls, although the difference was in significant. Conclusions : In conclusion, oral administration of CSB attenuates asthmatic manifestations including AHR ad airway recruitment of eosinophils in a mouse model which possibly results from selective inhibition of Th2 cell response to allergen. Our data suggest a potential clinical application of CSB for control of allergic asthma.

• 한국식품영양과학회지
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• 제43권9호
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• pp.1415-1422
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• 2014

항산화 활성이 강화된 발효유의 개발 가능성을 확인하고자 삼채 뿌리의 열수 추출조건을 설정한 후 열수 농축물을 첨가한 발효유를 제조하여 품질특성을 조사하였다. 추출 시간에 따른 열수 추출액의 항산화 활성은 $95^{\circ}C$에서 4시간 추출했을 때 DPPH radical 소거능이 54%, ABTS radical cation 소거능이 52%, reducing power가 0.79, metal chelating 활성이 62%로 가장 높게 나타났으며, 이들의 항산화 활성은 총 페놀성 화합물 및 총 플라보노이드 함량과 높은 상관계수(R)를 나타내었다. $95^{\circ}C$에서 4시간 추출한 열수 추출물을 1/10배로 농축한 농축물을 0~10%(w/w) 농도 범위로 첨가하여 발효한 발효유의 품질특성을 비교한 결과, 발효가 완료된 발효유의 pH는 6.57~6.60에서 4.34~4.51로 감소한 반면에 적정산도는 0.22~0.23%에서 1.01~1.10%로 증가되었고, 생균수가 6.40~6.80 log CFU/mL에서 8.60~9.20 log CFU/mL로 증가되었다. 삼채 뿌리 열수 농축물을 첨가한 발효유에 매실향을 첨가한 관능평가에서는 된 정도와 신맛의 강도를 제외한 갈색, 향 및 당도의 강도에서 삼채뿌리 열수 농축물의 첨가량이 증가함에 따라 각각 유의적 차이가 나타났으나, 2.5% 첨가구에서는 색, 향, 단맛 및 신맛의 강도뿐만 아니라 전반적 기호도에서도 대조구와 유의적 차이를 보이지 않았다.

• 한국식품영양과학회지
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• 제44권3호
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• pp.412-417
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• 2015

증포 처리가 삼채 뿌리의 품질 특성 및 항산화 활성에 미치는 영향을 조사하였다. 증포 처리한 삼채 뿌리는 생 삼채 뿌리보다 황 함량이 각각 2.0배와 2.1배 감소하였다. pH와 총당은 증포 처리에 의해 감소하였으나 적정산도와 갈변도는 증가하였다. 색도의 경우 L과 b 값은 감소하였으나 a 값은 증가하였다. 열수 추출물의 항산화 활성은 증포 4회가 DPPH radical assay, ABTS radical assay 및 reducing power에서 $EC_{50}$ 값이 각각 0.44 mg/mL, 9.01 mg/mL 및 0.48 mg/mL로 가장 낮게 나타났으나 TPC($34.47{\mu}g/mg$)와 갈변도(2.05 at 280 nm와 0.20 at 420 nm)는 가장 높게 나타났다. 이들 성분과 각 항산화 활성에 대한 $EC_{50}$ 값의 $R^2$는 0.87 이상의 높은 값을 나타내었다. 결과적으로 증포 처리는 삼채 뿌리의 항산화 활성을 증가시키는 데 효과적인 가공 방법으로 판단된다.

• 한국항해항만학회지
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• 제37권6호
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• pp.575-580
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• 2013

IMU(Inertial Measurement Unit)는 선박, 잠수함, 항공기 및 군용장비 응용분야에서 적용되어 자세 측정 영역에 주로 사용되고 있지만, 이런 IMU는 고가의 장비이기 때문에 특수 분야에서만 한정적으로 이용되어 왔다. 그러나 MEMS AHRS(MEMS : Micro Electro Mechanical System, AHRS : Attitude and Heading Reference System)의 현 기술 상황은 지능형 MEMS AHRS가 채택된 응용분야에서 가격이 높은 IMU를 대체 할 수 있는 수준에 이르고 있다. 본 논문에서는 자이로 센서, 가속도 센서, 지자기 센서를 사용한 AHRS를 이용하여 선박의 주요 운동 요소인 횡동요, 종동요, 선수동요 값을 측정할 수 있는 무선 선체 운동 측정 시스템을 개발하였다. 또한 AHRS 센서가 발생시키는 오차인 순간 가속도, 지자기의 영향 및 진동에 대응하기 위하여 칼만 필터링 기능이 탑재된 센서를 적용함으로서 최적의 성능을 실현하고자 하였다. 본 연구에서 구현한 AHRS 센서를 이용한 무선 선체 운동 측정 시스템을 이용하여 실선 실험을 실행하였으며, 선박의 제한적인 설치 상황에서도 편리하게 적용할 수 있을 것으로 보여진다. 향후 이 시스템이 선박에서 INS(Integrated Navigation System) 및 VDR(Voyage Data Recorder)과 같은 선박 장비와 호환되어 활용될 경우 항해 안전과 해양사고 분석에 유용하게 사용될 수 있을 것으로 판단된다.

• 한국측량학회:학술대회논문집
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• 한국측량학회 2004년도 춘계학술발표회논문집
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• pp.59-64
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• 2004

In this paper, the error compensation method of the low-cost IMU is proposed. In general, the position and attitude error calculated by accelerometers and gyros grows with time. Therefore the additional information is required to compensate the drift. The attitude angles can be bound accelerometer mixing algorithm and the heading angle can be aided by single antenna GPS velocity. The Kalman filter is used for error compensation. The result is verified by comparing with the attitude calculated by Attitude Heading Reference System with Micro Electro Mechanical System for a basis

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1992년도 한국자동제어학술회의논문집(국내학술편); KOEX, Seoul; 19-21 Oct. 1992
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• pp.135-140
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• 1992

This paper presents the configuration, HILS procedure and performance simulation results of the RPV autopilot including a strapdown AHRS. Real time hardware-in-the-loop simulation was performed by using a 3 axis flight motion simulator alonged assumed flight trajectory of the RPV. Being compared with the result of the 6 DOF simulation, the HILS results showed that the performance of the autopilot was satisfactory.

• 대한화학회지
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• 제55권2호
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• pp.308-312
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• 2011