• Journal of the Korean Magnetic Resonance Society
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• v.19 no.2
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• pp.95-98
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• 2015

Reverse gyrase is a hyperthermophile specific protein which introduces positive supercoils into DNA molecules. Reverse gyrase consists of an N-terminal helicase-like domain and a C-terminal topoisomerase domain. The helicase-like domain shares the three-dimensional structure with two tandem RecA-folds (H1 and H2), in which the subdomain H2 is interrupted by the latch domain (H3). To understand the physical property of the hyperthermophile-specific protein, two subdomains af_H1 and af_H23 have been cloned into E. coli expression vector, pET28a. The $^{15}N$-labeled af_H1 and af_H23 proteins were expressed and purified for heteronuclear NMR study. The af_H1 protein exhibits the well-dispersion of amide signals in its $^1H/^{15}N$-HSQC spectra and thus further NMR study continues to be progressed.

• The Korean Journal of Physiology and Pharmacology
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• v.1 no.1
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• pp.107-115
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• 1997

We studied the effects of bile acids on the induction ofapoptosis in HepG2 human hepatocellular carcinoma cells. Treatment with either ursodeoxycholic acid (UDCA) or lithocholic acid (LCA) resulted in a dose- and time-dependent decrease in cell viability assessed by MTT assay. Both UDCA and LCA also induced genomic DNA fragmentation, a hallmark of apoptosis, indicating that the mechanism by which these bile acids induce cell death was through apoptosis. Cycloheximide, a protein synthesis inhibitor, blocked the apoptosis induced by these bile acids, implying that new protein synthesis may be required for the apoptosis. Intracellular $Ca^{2+}$ release blockers (dantrolene and 3,4,5-trimethoxybenzoic acid-8-(diethylamino)octyl ester) inhibited decreased cell viability and DNA fragmentation induced by these bile acids. Treatment of HepG2 cells with calcium ionophore A23l87 induced DNA fragmentation. These results suggest that UDCA and LCA induce apoptosis in the HepG2 cells and that the activation of intracellular $Ca^{2+}$ signals may play an important role in the apoptosis induced by these bile acids.

• The Korean Journal of Physiology and Pharmacology
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• v.22 no.2
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• pp.193-201
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• 2018

Connective tissue growth factor (CTGF) is a novel fibrotic mediator, which is considered to mediate fibrosis through extracellular matrix (ECM) synthesis in diabetic cardiovascular complications. Statins have significant immunomodulatory effects and reduce vascular injury. We therefore examined whether fluvastatin has anti-fibrotic effects in vascular smooth muscle cells (VSMCs) and elucidated its putative transduction signals. We show that advanced glycation end products (AGEs) stimulated CTGF mRNA and protein expression in a time-dependent manner. AGE-induced CTGF expression was mediated via ERK1/2, JNK, and Egr-1 pathways, but not p38; consequently, cell proliferation and migration and ECM accumulation were regulated by CTGF signaling pathway. AGE-stimulated VSMC proliferation, migration, and ECM accumulation were blocked by fluvastatin. However, the inhibitory effect of fluvastatin was restored by administration of CTGF recombinant protein. AGE-induced VSMC proliferation was dependent on cell cycle arrest, thereby increasing G1/G0 phase. Fluvastatin repressed cell cycle regulatory genes cyclin D1 and Cdk4 and augmented cyclin-dependent kinase inhibitors p27 and p21 in AGE-induced VSMCs. Taken together, fluvastatin suppressed AGE-induced VSMC proliferation, migration, and ECM accumulation by targeting CTGF signaling mechanism. These findings might be evidence for CTGF as a potential therapeutic target in diabetic vasculature complication.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2000.11a
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• pp.888-891
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• 2000

Drilling is one of the most important operations in machining industry and usually the most efficient and economical method of cutting a hole in metal. From automobile parts to aircraft components, almost every manufactured product requires that holes are to be drilled for the purpose of assembly, creation of fluid passages, and so on. It is therefore desirable to monitor drill wear and hole quality changes during the hole drilling process. One important aspect in controlling the drilling process is drill wear status monitoring. With the monitoring, we may decide on optimal timing for tool change. The necessity of the detection of tool wear, fracture and the abnormal tool state has been emphasized in the machining process. Accordingly, this paper deals with the cutting characteristics of the hot-rolled high strength steels using common HSS drill. The performance variables include drill wear data obtained from drilling experiments conducted on the workpiece. The results are obtained from monitoring of the cutting force and Acoustic Emission (AE) signals, and from the detection of the abnormal tool state with the computer vision system.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.03a
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• pp.42-50
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• 2008

To evaluate rock bolt integrity, destructive test such as pull-out test has been commonly carried out. This method is known as time consuming, expensive, and inaccurate procedure. To improve destructive method, non-destructive techniques using transmitted guided ultrasonic waves were suggested. Note for the transmission method, the source for the generation of ultrasonic waves should be installed during the rock bolt construction. The purpose of this study is to investigate the reflection method using reflected guided ultrasonic waves to evaluate the integrity of the rock bolt grouted, and to compare the results evaluated by the reflection and transmission methods. The guided waves are generated by PZT element and received by AE sensor. The measured signals are analyzed by the wavelet transform. The results show that the energy velocities of guided ultrasonic waves increase with the defect ratio in both transmission and reflection method. The reflection method produces the lower velocity in all defect ratio. This research demonstrates that the reflection method may be suitable and easer method for the field tests.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.12 no.6
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• pp.1-7
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• 2003

The experimental research was conducted to find an end mill with an ideal helix angle, which has a superior anti-vibration effect and a low machining tolerance. A conventional endmill which all low blades are $30^{\circ}$ helix angles and a different helix angle endmill which the opposite two blades are $30^{\circ}$ and the other opposites are different helix angles were studied. The cutting farce, machining tolerance and surface roughness were obtained. The AE signals appeared to have low values in up-milling rather than in down-milling. These are also appeared to have low values at low spindle revolutions rates. The cutting force values of Fxy and Fxyz were found to be increased according to the value of helix angle. In up-milling, it was difficult to find a definite tendency in machining tolerance, but in down-milling machining tolerance of the different helix angle end mill was found to be lower than that of the convention end mill. There is a definite tendency that the surface roughness gets better as the RPM increases. In down-milling, Type A($25^{\circ}$＋$30^{\circ}$) appeared to bring the most satisfactory result.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2003.04a
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• pp.196-201
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• 2003

The experimental research was conducted to find an end mill with an ideal helix angle, which has a superior anti-vibration effect and a low machining-tolerance. A conventional endmill which all four blades are $30^\circ$ helix angles and a different helix angle endmill which the opposite two blades are $30^\circ$ and the other opposites are different helix angles were studied. The cutting force, machining tolerance and surface roughness were obtained. The AE signals appeared to have low values in up-milling rather than in down-milling. These are also appeared to have low values at low spindle revolutions rates. The cutting force values of Fxy and Fxyz were found to be increased according to the value of helix angle. In up-milling, it was difficult to find a definite tendency in machining tolerance, but in down-milling, machining tolerance of the different helix angle end mill was found to be lower than that of the convention end mill. There is a definite tendency that the surface roughness gets better as the RPM increases. In down-milling, Type $A(25^\circ+30^\circ)$ appeared to bring the most satisfactory result.

• Bulletin of the Korean Chemical Society
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• v.31 no.6
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• pp.1527-1534
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• 2010

The conventional peptide modification process of guanidination, in which the amino groups of lysine residues are converted to guanidino groups using O-methylisourea to create more basic homoarginine residues, is often used to improve the signal intensity of lysine-containing peptides in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Here, we used three different protease enzymes (trypsin, Lys-C, and Glu-C) to evaluate the effects of guanidination on the MS signals of two enzymatically digested proteins. Horse heart myoglobin and bovine serum albumin were guanidinated either before or after digestion with trypsin, Lys-C, or Glu-C. The resulting peptides were subjected to MALDI-MS, and signal intensities and sequence coverage were systematically evaluated for each digest. Guanidination prior to Glu-C digestion improved sequence coverage for both proteins. For myoglobin, guanidination before enzymatic digestion with trypsin or Lys-C also enhanced sequence coverage, but guanidination after enzymatic digestion enhanced sequence coverage only with Lys-C. For albumin, guanidination either before or after Glu-C digestion increased sequence coverage, whereas pre- or post-digestion guanidination decreased sequence coverage with trypsin and Lys-C. The amino acid composition of a protein appears to be the major factor determining whether guanidination will enhance its MALDI-MS sequence coverage.

• Proceedings of the KAIS Fall Conference
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• 2003.11a
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• pp.141-148
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• 2003

Customer retention is a common concern for many industries and a critical issue for the survival in today's greatly compressed marketplace. Current customer retention models only focus on detection of potential defectors based on the likelihood of defection by using demographic and customer profile information. In this paper, we propose a dynamic procedure for defection detection and prevention using past and current customer behavior by utilizing SOM and Markov chain. The basic idea originates from the observation that a customer has a tendency to change his behavior (i.e. trim-out his usage volumes) before his eventual withdrawal. This gradual pulling out process offers the company the opportunity to detect the defection signals. With this approach, we have two significant benefits compared with existing defection detection studies. First, our procedure can predict when the potential defectors could withdraw and this feature helps to give marketing managers ample lead-time for preparing defection prevention plans. The second benefit is that our approach can provide a procedure for not only defection detection but also defection prevention, which could suggest the desirable behavior state for the next period so as to lower the likelihood of defection. We applied our dynamic procedure for defection detection and prevention to the online gaming industry. Our suggested procedure could predict potential defectors without deterioration of prediction accuracy compared to that of the MLP neural network and DT.

• Geomechanics and Engineering
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• v.18 no.5
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• pp.481-492
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• 2019

Due to the underground openings, the tangentially concentrated stress of the tunnel remains larger at excavation boundary and decreases toward the interior of the surrounding rock with a certain gradient. In order to study the effect of different gradient stress on rockburst, the true-triaxial gradient and hydraulic-pneumatic combined test apparatus were carried out to simulate the rockburst processes. Under the different gradient stress conditions, the rock-like specimen (gypsum) was tested independently through three principal stress directions loading--fast unloading of single surface--top gradient and hydraulic-pneumatic combined loading, which systematically analyzed the macro-mesoscopic damage phenomena, force characteristics and acoustic emission (AE) signals of the specimen during rockburst. The experimental results indicated that the rockburst test under the gradient and hydraulic-pneumatic combined loading conditions could perfectly reflect the rockburst processes and their stress characteristics; Relatively high stress loading could cause specimen failure, but could not determine its mode. The rockburst under the action of gradient stress suggested that the failure mode of specimen mainly depended on the stress gradient. When the stress gradient was lower, progressive and static spalling failure occured and the rockburst grades were relatively slight. On the other hand, shear fractures occurred in rockbursts accounted for increasingly large proportion as the stress gradient increased and the rockburst occurred more intensely and suddenly, the progressive failure process became unconspicuous, and the rockburst grades were moderate or even stronger.