• 제목/요약/키워드: ADAM 10

검색결과 238건 처리시간 0.025초

A New Protein of ${\alpha}$-Amylase Activity from Lactococcus lactis

  • Wasko, Adam;Polak-Berecka, Magdalena;Targonski, Zdzislaw
    • Journal of Microbiology and Biotechnology
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    • 제20권9호
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    • pp.1307-1313
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    • 2010
  • An extracellular ${\alpha}$-amylase from Lactococcus lactis IBB500 was purified and characterized. The optimum conditions for the enzyme activity were a pH of 4.5, temperature of $35^{\circ}C$, and enzyme molecular mass of 121 kDa. The genome analysis and a plasmid curing experiment indicated that $amy^+$ genes were located in a plasmid of 30 kb. An analysis of the phylogenetic relationships strongly supported a hypothesis of horizontal gene transfer. A strong homology was found for the peptides with the sequence of ${\alpha}$-amylases from Ralstonia pikettii and Ralstonia solanacearum. The protein with ${\alpha}$-amylase activity purified in this study is the first one described for the Lactococcus lactis species, and this paper is the first report on a Lactococcus lactis strain belonging to the amylolytic lactic acid bacteria (ALAB).

High-Resolution 3-D Refractive Index Tomography and 2-D Synthetic Aperture Imaging of Live Phytoplankton

  • Lee, SangYun;Kim, Kyoohyun;Mubarok, Adam;Panduwirawan, Adisetyo;Lee, KyeoReh;Lee, Shinhwa;Park, HyunJoo;Park, YongKeun
    • Journal of the Optical Society of Korea
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    • 제18권6호
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    • pp.691-697
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    • 2014
  • Optical measurements of the morphological and biochemical imaging of phytoplankton are presented. Employing quantitative phase imaging techniques, 3-D refractive index maps and high-resolution 2-D quantitative phase images of individual live phytoplankton are simultaneously obtained without exogenous labeling agents. In addition, biochemical information of individual phytoplankton including volume, mass, and density of individual phytoplankton are also quantitatively obtained from the measured refractive index distributions. We expect the present method to become a powerful tool for the study of phytoplankton.

Interaction between thyroglobulin and ADAMTS16 in premature ovarian failure

  • Pyun, Jung-A;Kim, Sunshin;Kwack, KyuBum
    • Clinical and Experimental Reproductive Medicine
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    • 제41권3호
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    • pp.120-124
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    • 2014
  • Objective: The aim of the present study was to examine whether interactions between polymorphisms in the thyroglobulin and ADAM metallopeptidase with thrombospondin type 1 motif, 16 (ADAMTS16) genes are associated with the development of premature ovarian failure (POF). Methods: A total of 75 patients with POF and 196 controls were involved in this study. We used a GoldenGate assay to genotype single nucleotide polymorphisms (SNPs). Logistic regression analysis was performed to identify POF-associated polymorphisms and synergistic interactions between polymorphisms in the thyroglobulin and ADAMTS16 genes. Results: Single gene analyses using logistic regression analysis showed no significant association between polymorphisms in the two genes and POF. In the results from interaction analyses, we found seven synergistic interactions between the polymorphisms in thyroglobulin and ADAMTS16, although there was no combination showing p-values lower than the significant threshold using the Bonferroni correction. When the AG genotype was present at the rs853326 missense SNP, the A and G alleles at the tagging SNPs rs16875268 and rs13168665 showed significant interactions (odds ratios=5.318 and 16.2 respectively; 95% confidence intervals, 1.64-17.28 and 2.08-126.4; p=0.0054 and 0.0079). Conclusion: Synergistic interactions between polymorphisms in the thyroglobulin and ADAMTS16 genes were associated with an increased risk of POF development in Korean women.

복합재료를 적용한 항공기용 카메라 구조 경량화 설계 및 최적조건 선정에 관한 연구 (A Study on the Vibration Analysis and Optimization for the Composite Optical Structure of an Aircraft)

  • 김병준;이준호;이행복;정대윤;전성식
    • Composites Research
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    • 제25권6호
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    • pp.230-235
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    • 2012
  • 본 연구에서는 항공기용 카메라구조의 진동특성 파악하고 최적조건을 선정하여 구조의 경량화 설계 방안을 제시하였다. 다물체 동력학 프로그램인 ADAMS를 이용하여 모드해석을 진행하고, 해석 결과를 가지고 직교법을 이용하여 항공기용 카메라 구조의 경량화 및 최적조건을 선정하여 이전의 항공기용 카메라 구조와 모드해석 결과를 서로 비교하였다. 또한, 기존에 제시 되어진 베어링의 차원의 값을 무차원의 값으로 전환하여 보다 효과적인 베어링의 기준을 선정하였으며, 비교된 결과를 토대로 최적화된 항공기용 카메라 구조가 제안되었다.

Effects of the Jinan Red Ginseng Extract Treatment on Poloxamer 407-induced Hyperlipidemia in Rabbits

  • Choi, Kyung-Min;Lee, Jeong Ho;Adam, Gareeballah Osman;Kim, Shang-Jin;Kang, Hyung-Sub;Yang, Yeong-Seok;Kim, Gi-Beum
    • 한국자원식물학회지
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    • 제30권6호
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    • pp.601-611
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    • 2017
  • Hyperlipidemia is an increase in one or more of the plasma lipids, including triglycerides, cholesterol. Ginseng has been used as a valuable tonic and for the treatment of various diseases. The objective of this study was to evaluate the effects of Jinan red ginseng (JRG) water extract on the blood and serum in rabbits with hyperlipidemia induced by poloxamer 407 when supplied in drinking water. JRG treatment was performed for 20 weeks. We evaluated the effects of the JRG treatment on diabetes through hematological and biochemical analysis before and after JRG treatment were performed. Our results indicate that LDL, total cholesterol, and triglycerides were significantly decreased compared prior JRG supply. CRE, BUN, CK and UA levels indicating renal functions are significantly reduced when compared to those prior to the JRG supply. In addition, AST, ALT, ALP, and LDH were significantly reduced indicating hepatoprotective effect. Blood electrolytes deteriorated in HL rabbits were improved when JRG supplied. In conclusion, Biochemical and hematological analysis demonstrate that the JRG is effective to alleviate the hyperlipidemia signs.

Robust Nonlinear Control of AC Brushless Motor for Electric Vehicles Application

  • Langarica-Cordoba, Diego;Guerrero-Ramirez, Gerardo V.;Claudio-Sanchez, Abraham;Duran-Fonseca, Miguel A.;Adam-Medina, Manuel;Astorga-Zaragoza, Carlos-Manuel
    • Journal of Power Electronics
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    • 제11권4호
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    • pp.430-438
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    • 2011
  • This article proposes a robust nonlinear control based on Lyapunov's redesign, whose purpose is to deal with parametric uncertainty in the resistance of the motor windings. The robust controller design is based on the passivity properties of the motor, as well as energy shaping and damping injection. The application of this control technique is focused on electric vehicles mainly formed by a battery bank, a power inverter, an AC brushless motor and the mechanical transmission. The sine PWM technique is used to trigger the switching devices of inverter. The results were obtained from simulation, where is shown that robust control makes a proper tracking of electromagnetic torque.

윤활제의 분자구조가 Base Wax-pigment Composite의 UV-absorption특성에 미치는 영향에 관한 연구 (Influence of Molecular structure of Lubricants on the UV-absorption Characteristics of the Base Wax-pigment Composites)

  • 김승환
    • 한국응용과학기술학회지
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    • 제2권2호
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    • pp.47-53
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    • 1985
  • Correlation among the wax-pigment composites which is base vehicles for the crepas was investigated in terms of fadeness. The base wax synthesized and pigments are compounded with petroleum lubricant and exposed under carbon arc individually. The yellowing phenomenon was appeared on the reference papers coated with the spindle oil which was then exposed. The papers were again extracted with distilled water and pH of them were ranged between 6.2-6.5. Color difference from Adam-Nickerson equation, ${\vartriangle}E$ of base wax is 0.15 and that of spindle oil are varied from 0.66 to 15.62. Since the main components of the petroleum lubricant are aromatic hydrocarbons which have absorptions characteric of UV ranging from 240 to 280 nm, fadeness characteristics of the composites are largely depend upon the change of molecular structure of spindle oil by absorbing UV. Thus the spindle oil having the following physical properties has the better resistance of fadness and is recommended to use in compounding the base wax-pigment composites: ${\cdot}$ main component: paraffinic hydrocarbon ${\cdot}$ pour point: below - $15^{\circ}C$ ${\cdot}$ UV absorption characteristics: ${\lambda}max.$ : 268-290nm ${\cdot}$ absorbancy: below 0.1(0.03ml of sample/50ml of $CHCl_3$)

Behaviour of axially loaded RC columns strengthened by steel angles and strips

  • Adam, J.M.;Ivorra, S.;Gimenez, E.;Moragues, J.J.;Miguel, P.;Miragall, C.;Calderon, P.A.
    • Steel and Composite Structures
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    • 제7권5호
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    • pp.405-419
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    • 2007
  • This paper presents the development of some numerical models based on the results of laboratory tests performed on axially loaded RC columns strengthened with steel angles and strips. These numerical models consider the nonlinearity of the building materials and the effects of the contact interfaces between different materials. The results of the finite element models accurately describe the general behaviour of the strengthened columns. This study allows engineers to assess the relative importance of the mechanisms acting on the strengthened RC columns. Constructive recommendations are also provided in this paper.

Phytogenic silver nanoparticles (Alstonia scholaris) incorporated with epoxy coating on PVC materials and their biofilm degradation studies

  • Supraja, Nookala;Tollamadugu, Naga Venkata Krishna Vara Prasad;Adam, S.
    • Advances in nano research
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    • 제4권4호
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    • pp.281-294
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    • 2016
  • The advantages of nano-scale materials (size 1-99 nm in at least in one dimension) could be realized with their potential applications in diversified avenues. Herein, we report for the first time on the successful synthesis of homogeneous epoxy coatings containing phytogenic silver nanoparticles (Ag) on PVC and glass substrates by room-temperature curing of fully mixed epoxy slurry diluted by acetone. Alstonia scholaris bark extract was used to reduce and stabilize the silver ions. The surface morphology and mechanical properties of these coatings were characterized using the techniques like, UV-Vis (UV-Visible) spectrophotometry, X-ray diffraction (XRD), Fourier transform infrared spectrophotometry (FT-IR), Epifluorescence microscopy and scanning electron microscopy (SEM). The effect of incorporating Ag nanoparticles on the biofilm (scale) resistant epoxy-coated PVC was investigated by total viable counts ($CFU/cm^2$) from epoxy coating from (Initial) $1^{st}$ day to $25^{th}$ days. The phytogenic Ag nanoparticles were found to be significantly improving the microstructure of the coating matrix and thus enhanced the anti-biofilm performance of the epoxy coating. In addition, the antimicrobial mechanism of Ag nanoparticles played an important role in improving the anti-biofilm performance of these epoxy coatings.

Ethnobotany of Wild Baobab (Adansonia digitata L.): A Way Forward for Species Domestication and Conservation in Sudan

  • Gurashi, N.A.;Kordofani, M.A.Y.;Adam, Y.O.
    • Journal of Forest and Environmental Science
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    • 제33권4호
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    • pp.270-280
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    • 2017
  • Selection of superior phenotypes of fruit trees and products based on established criteria by local people is a prerequisite for future species domestication and conservation. Thus the study objective was to identify the local people's perceptions and preferences on baobab trees and products. A sample of 142 respondents was randomly selected using structured interviews in Blue Nile and North Kordofan, Sudan in 2013. Descriptive analysis was employed using SPSS and Excel programs. The study results indicated that local people use the morphological characteristics of the tree (leaves, fruits, seeds, kernels and bark) to differentiate individual trees. Based on the perceptions, local people recorded trees with delicious leaves, white pulp color, big fruit size and mature capsule size, and high pulp yield as criteria for differentiating between baobab trees in the study areas. In contrast, the undesirable traits were connected to trees with acidic pulp, slimy pulp, bitter leaves, and low pulp yield. The study concluded that the ethnobotanical knowledge of the baobab tree and its products may play an important role in tree domestication and improvement in Sudan. However, further research on tree genetics is needed to complement the ethnobotanical knowledge for baobab resources domestication and conservation.