• Journal of Plant Biology
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• v.35 no.1
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• pp.25-36
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• 1992

The effects of amphotericin B ($150\;\mu\textrm{g}/ml$) and cycloheximide ($10\;\mu\textrm{g}/ml$) on the biosynthesis of phospholipid and the composition of fatty acids in chloroplasts isolated from ChZorella were analyzed. The contents of the total lipid and phospholipid (PC, PE, PI) in treatment with antibiotics were lower compared with the control. In the whole cell system, the major fatty acids utilized for biosynthesis of phospholipid were palmitic acid (31.96%) and linoleic acid (16.96%) in control while those were palmitic acid (36.15%) and linolenic acid (16.71%) in treatment with amphotericin B. And in treatment with cycloheximide, palmitic acid (31.90%) and stearic acid (15.32%) were used in phospholipid formation. The major fatty acids in chloroplasts were analyzed as to be palmitic acid and linolenic acid in control (33.75%, 18.90%) and in treatment with amphotericin B (36.75%, 9.46%). However, it was shown that the major fatty acids in chloroplasts treated with cycloheximide were palmitic acid (28.01%) and oleic acid (19.27%).9.27%).

• Culinary science and hospitality research
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• v.4
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• pp.413-436
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• 1998

In order to guarantee the safety of Korean Naeng Myon Broth in summer, pH and micrological quality of iced noodle gravy which is cooked and sold in public restaurant were investigated. And the variations of pH, taste and microorganism were studied with organic acid treatment during storage period. The micrological status of iced noodle gravy showed that average number of total aerobic bacteria was 2.7${\times}$105CFU/$m\ell$ and fecal coliform was 8.3${\times}$103CFU/$m\ell$ respectly. Food poison microorganisms not detected and average of pH was 4.8. pH changed slightly during storage period and deceased with increasement of organic acid added. Sour taste of organic acid increased acetic acid, citric acid, lactic acid order and there was no organic acid having with more sour taste than iced noodle gravy which is cooked and sold in public restaurant. Salmonella was destroyed when treated with 1% of acetic acid and lactic acid and Escherichia coli became extinct at treatment with 0.8% acetic acid and 1% lactic acid. in case of same concentration, inhibition effects to Escherichia coli multiplication show citric acid, lactic acid, acetic acid order. In conclusion, treatment with more than 0.8% acetic acid and above 1.0% lactic acid or more than 1.0% citric acid in case of production and consumption a day can prevent from Escherichia coli pollution in Korean Naeng Myon Broth.

• Korean Journal of Organic Agriculture
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• v.24 no.3
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• pp.453-463
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• 2016

This study was undertaken to investigate the effects of dicarboxylic acid supplementation, as replacement antibiotics, of on in vitro ruminal parameters and milk yield and milk composition in lactating cows. in vitro treatments were 1) Con (4 g of basal diet), 2) CM (4 g of basal diet + 0.05 ml of monensin), 3) CR (4 g of basal diet + 0.1 ml of dicarboxylic acid) and in vivo treatments were 1) Con (25 kg of basal diet/head/day), and 2) CR (25 kg of basal diet + 5 g of dicarboxylic acid/head/day), respectively. A total 10 lactating dairy cows ($649{\pm}19kg$ average body weight, $99{\pm}65$ average milking days) were divided in to two groups according to mean milk yield and number of days of postpartum. The cows fed a basal diet during adaptation (2 wk) and experimental diets during the treatment periods (4 wk). In the first in vitro experiment, there were no statistical differences between treatments in pH, gas production, and ammonia-N and lactic acid concentration during incubation. However, dry matter digestibility was significantly higher in CR treatment compared to control or CM treatment (P<0.05). Total VFA was tended to higher in CR treatment than those of control and CM treatment (P>0.05). In the second experiment, milk yield was significantly higher in treatment (40.39 kg) compared to control (35.19 kg), (P<0.05). Milk composition and MUN were not changed by dietary supplementing dicarboxylic acid. Therefore the present results reporting that supplementation of dicarboxylic acid might enhance the stabilization of ruminal fermentation and increase the milk yield of lactating cows.

• Food Science of Animal Resources
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• v.25 no.2
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• pp.162-167
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• 2005

This study was carried out to investigate the effect of ultrasound treatment on the quality, amino acid and fatty acid composition of fried chicken meat The moisture content of raw chicken meat was higher than fried chicken meat, but the crude protein and fat were lower than those of fried chicken meat The moisture and crude fat of ultrasonic fried chicken meat were higher than those of control. The crude protein of breast meat was higher than leg meat, but the moisture and crude fat were lower than leg meat The ultrasonic treatment did not affect on calorie of the fried chicken meat The pH of leg meat was higher than breast meat, and the fried loss of breast meat was higher than leg meat Frying loss of the leg meat was higher than that of control when ultrasonic treated The Hunters $L^{\ast}$ value of ultrasonic treated breast meat was higher than control, but the leg meat were not significantly different between ultrasonic treatment and control. The $a^{\ast}$ value of leg meat was higher than breast meat, and $b^{\ast}$ value of breast meat was higher than leg meat And the $b^{\ast}$ value of ultrasonic treatment was higher than control. The glutamic acid and aspartic acid were major amino acids in chicken meat The palmitic acid $(C_{16:0})$ and oleic acid $(C_{18:1})$ by fried were decreased, and the linoleic acid $(C_{18:2})$ was increased But the fatty acid composition by ultrasonic treatment were not changed.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.23 no.1
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• pp.65-69
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• 2010

In this study, we compared two methods(an acid treatment in strong acid reflux and a heat treatment in air atmosphere) for hydrophilic surface treatment of multi-walled carbon nanotubes(MWNT) to enhance the capcity of $RuO_2$/MWNT composite electrode materials for ultracapacitor. Both treatments generated a number of defects on the surface of MWNT by the breakage of $\pi$ bond in graphene layer at which carboxyl groups were introduced. However, the degree of hydrophilicity generated by strong acid treatment was higher than that by heat treatment in air, which was revealed by the quantitative measurement of surface carboxyl groups by using Boehm titration. The increased hydrophilicity save rise to an improved dispersity of $RuO_2$ nanoparticles on MWNT. Finally, the improved dispersity resulted in the capacity enhancement of composite electrode materials for ultracapacitor.

• Journal of Plant Biology
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• v.35 no.4
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• pp.371-383
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• 1992

The effects of IAA $(10^{-3}M)\;an;GA_3\;(2{\times}10^{-8}\;M)$ on the biosynthesis of phospholipids and their fatty acid compositions in chloroplast isolated from Chlorella ellipsidea were analyzed. Growth ratio and the levels of total lipids and phospholipids in whole cell system and chloroplast treated with phytohormones were higher than those of control, and when treated with GAl. phosphatidylcholine content was predominantly higher than that of control. It was showed that in whole cell system the fatty acid utilized for biosynthesis of phopholipids was 27.43% for palmitic acid in control, while that was 20.25% for linolenic acid in treatment with 1M. In treatment with $GA_3$, 23.17% for linolenic acid was used in phospholipid formation. The major fatty acid in the chloroplst system was analyzed to be 35.67% for palmitic acid in control and in treatment with 1M, 24.91% for linolenic acid was used in phospholipid formation, while in treatment with $GA_3$, major fatty acid of phospholipids was 22.80% for linoleic acid.c acid.

• Nutrition Research and Practice
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• v.1 no.2
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• pp.105-112
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• 2007

Ascorbic acid has been reported to extend replicative life span of human embryonic fibroblast (HEF). Since the detailed molecular mechanism of this phenomenon has not been investigated, we attempted to elucidate. Continuous treatment of HEF cells with ascorbic acid at ($200{\mu}M$) from 40 population doubling (PD) increased maximum PD numbers by 18% and lowered $SA-{\beta}-gal$ positive staining, an aging marker, by 2.3 folds, indicating that ascorbic acid extends replicative life span of HEF cells. Ascorbic acid treatment lowered DCFH by about 7 folds and Rho123 by about 70%, suggesting that ascorbic acid dramatically decreased ROS formation. Ascorbic acid also increased aconitase activity, a marker of mitochondrial aging, by 41%, indicating that ascorbic acid treatment restores age-related decline of mitochondrial function. Cell cycle analysis by flow cytometry revealed that ascorbic acid treatment decreased G1 population up to 12%. Further western blot analysis showed that ascorbic acid treatment decreased levels of p53, phospho-p53 at ser 15, and p21, indicating that ascorbic acid relieved senescence-related G1 arrest. Analysis of AP (apurinic/apyrimidinic) sites showed that ascorbic acid treatment decreased AP site formation by 35%. We also tested the effect of hydrogen peroxide treatment, as an additional oxidative stress. Continuous treatment of $20{\mu}M$ of hydrogen peroxide from PD 40 of HEF cells resulted in premature senescence due to increased ROS level, and increased AP sites. Taken together, the results suggest that ascorbic acid extends replicative life span of HEF cells by reducing mitochondrial and DNA damages through lowering cellular ROS.

• Journal of Sericultural and Entomological Science
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• v.17 no.1
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• pp.35-39
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• 1975

The acid treatment hatching method has been used practically for about 60 years and a number of investigators have studied about the artificial hatching for silkworm e99s, but the basic theory about the acid treatment hatching is not clarified yet. It is no exaggeration to say that the accidents of non hatching is continued ceaselessly in the silkworm egg by hydrochloric acid treatment. It is believed that the accident is due to the adulterants in HCl lather than inattention of acid treatment. Therefore, the authors mixed hydrochloric acid (analytical grade) with or added it to chemical ingredients which are possible to be included in the process of hydrochloric acid production, and treated it to summer and fall silkworm egg. The metalic adulterants such as iron, mercury, lead and arsenic are appeared not to be worried, but damage of SO$_3$ and free chlorine is seemed to lie considerable. Therefore, before acid treatment for hatching hydrochloric acid was warmed to 50$^{\circ}C$ with shaking to evaporate several injurious gases, by whick the damage due to use of hydrochloric acid for acid treatment hatching is prevented considerably. In conclusion, it is recommended to pretest bioassay with every HCl samples before artificial hatching of silkworm egg.

• Journal of Adhesion and Interface
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• v.12 no.1
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• pp.26-33
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• 2011

Phosphoric acid treatments were conducted to improve the adhesion property of polyketone film to rubber. The effects of phosphoric acid treatments were characterized by using a contact angle analyzer and a XPS (X-ray photoelectron spectroscopy). Morphological changes were observed by using a scanning electron microscope (SEM) and an atomic force microscope (AFM) as the acid treatment condition varied in concentration and time. The contact angle was found to significantly decrease with the acid treatment. According to the XPS, increased wettability was attributed to the inclusion of oxygen containing groups such as hydroxyl, carbonyl and carboxyl by acid treatments. Cracks and pores were produced on the polyketone film surface and thus, roughness increased with the acid treatment. Interfacial adhesion strength between polyketone and natural rubber was largely improved by acid treatment due to the increased wettability and roughness of the polyketone surface. However, the higher level of acid treatment caused the degradation of the polyketone surface, and thus, its interfacial adhesion consequently decreased.

• Journal of Ginseng Research
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• v.35 no.4
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• pp.497-503
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• 2011

In order to enhance bioactive functionalities of ginseng, an acid impregnation processing was applied as a pre-treatment in producing red ginseng. Acid impregnation studies were conducted, and acids (ascorbic, malic, and citric acid) were selected. The optimal concentration of each acid was investigated in this study in terms of ginsenoside contents. The most concerned ginsenoside, $Rg_3$ was increased by ascorbic, malic, and citric acid pre-treated red ginseng up to 1 M acid concentration. In the case of ascorbic acid pre-treated red ginseng, $Rg_2$ concentration was increased depending on acid concentrations. Citric acid pre-treatment enhanced $Rg_2$, $Rg_3$, and $Rh_1+Rh_2$ formation in red ginseng. Therefore, ginsenoside patterns in red ginseng could be changed by acid impregnation pre-treatment depending on acid concentration and acid types. This research is expected to contribute to the development of the ginseng industry via new red ginseng products with selective and intensified functionality.