Effects of Adulterants in HCl on Artificial Hatching in the Silkworm Eggs

염산의 협잡물이 잠종의 인공부화에 미치는 영향

The acid treatment hatching method has been used practically for about 60 years and a number of investigators have studied about the artificial hatching for silkworm e99s, but the basic theory about the acid treatment hatching is not clarified yet. It is no exaggeration to say that the accidents of non hatching is continued ceaselessly in the silkworm egg by hydrochloric acid treatment. It is believed that the accident is due to the adulterants in HCl lather than inattention of acid treatment. Therefore, the authors mixed hydrochloric acid (analytical grade) with or added it to chemical ingredients which are possible to be included in the process of hydrochloric acid production, and treated it to summer and fall silkworm egg. The metalic adulterants such as iron, mercury, lead and arsenic are appeared not to be worried, but damage of SO$_3$ and free chlorine is seemed to lie considerable. Therefore, before acid treatment for hatching hydrochloric acid was warmed to 50$^{\circ}C$ with shaking to evaporate several injurious gases, by whick the damage due to use of hydrochloric acid for acid treatment hatching is prevented considerably. In conclusion, it is recommended to pretest bioassay with every HCl samples before artificial hatching of silkworm egg.

염산 제조과정에서 생성될 가능성이 있는 협잡물이나 침산과정에서 혼합될 수 있는 혼합물이 침산방법에 의한 가잠의 인공부화에 어떠한 영향을 미치는 가를 조사한 결과 철, 수은, 연, 비색 등은 부화에 별다른 영향이 없었으나 아황산(SO$_3$)과 유리염소(Cl)는 대단히 유해한 것으로 밝혀졌다. 따라서 침산시 염산을 5$0^{\circ}C$로 가열하여 각종 유해 개스를 제거할 후 사용하는 것이 좋으며 보다 안전한 인공부과를 기하기 위해서는 여러가지 염산표준액을 만들어 예비침산을 행한 후 본침산을 하는 것이 바람직하다.