• Title/Summary/Keyword: ABTS+ radical

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Chemical Components and Anti-oxidant Activities of Black Currant (블랙 커런트의 화학성분 및 항산화 활성)

  • Jeong, Chang-Ho;Jang, Chi-Weon;Lee, Koo-Yul;Kim, Il-Hun;Shim, Ki-Hwan
    • Food Science and Preservation
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    • v.19 no.2
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    • pp.263-270
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    • 2012
  • The chemical components and anti-oxidant activities of black currant were investigated. The pH, soluble solid and total acidity values were 3.36, 15.11 $^{\circ}Brix$, and 1.65%, respectively. The Hunter L, a, and b values were 18.20, 5.13, and 1.08, respectively. The proximate compositions were as follows; moisture, 77.64%; nitrogen free extract, 17.41%; crude fiber, 3.08%; crude protein, 1.28%; crude ash, 0.31%; and crude lipid, 0.28%, respectively. The mineral elements were K (177.36 mg/100 g), P (54.74 mg/100 g), and Ca (26.45 mg/100 g). The free sugar components were glucose (7.71%) and fructose (5.88%). The amino acid contents of the black currant were very rich in glutamic acid (105.73 mg/100 g) and deficient in cystine (5.29 mg/100 g). The ascorbic acid and total phenolic contents were 112.19 mg/100 g and 34.48 mg GAE/g, respectively. The ABTS and DPPH radical scavenging activity levels were 99.48% and 89.03% at the 10 and 1.25 mg/mL concentrations. The reducing power and FRAP of the black currant were dose-dependent. Thus, black currant can be an effective source of functional food substances, i.e., natural anti-oxidants.

Physicochemical of burdock (Arctium lappa L) tea depending on steaming and roasting treatment (증숙 및 볶음처리에 따른 우엉차의 품질특성)

  • Kwon, Yu-Ri;Youn, Kwang-Sup
    • Food Science and Preservation
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    • v.21 no.5
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    • pp.646-651
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    • 2014
  • To evaluate the processing adaptability of burdock (Arctium lappa L) tea, the physicochemical properties of burdock tea treated with steaming and roasting were investigated. The tea treatments included hot-air drying after roasting (HDR, drying at $60^{\circ}C$ for 6 hr), steaming after hot-air drying (SHD, steaming at $100^{\circ}C$ for 10 min), and steaming and then hot-air drying followed by roasting (SHDR, roasting at $250^{\circ}C$ for 3 min). The physicochemical properties, antioxidant activities, and sensory properties of the HDR, SHD, and SHDR teas were measured in hot water, and the tea was extracted from each sample. The color values of lightness (L) and yellowness (b) were lower in the HDR and SHDR teas, and the total sugar content of the SHDR tea was significantly higher than that of the other teas. The ABTS and DPPH radical scavenging abilities of the SHDR tea with a 500 mg% concentration were significantly higher than those of the SHD tea. In the sensory evaluation, the SHDR tea was ranked significantly higher than the other samples as to its color, flavor, taste, and overall acceptability. The results suggest that SHDR can be applied to burdock tea to achieve high quality and functionality.

Functional evaluation of marine micro-algae Amphidinium carterae extract (해양 미세조류 Amphidinium carterae 추출물의 기능성 평가)

  • Kim, Hae-Mi;Oh, Hyeonhwa;Jeong, Jong Hoon;Lee, Sang-Cheon;Moon, Hye-Jung;Jeong, Yong-Seob
    • Food Science and Preservation
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    • v.24 no.5
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    • pp.673-679
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    • 2017
  • In this study, the antimicrobial, antioxidant activities and ${\alpha}$-glucosidase inhibitory activities of Amphidinium carterae ethanol extract (AE) was evaluated for using as a functional food ingredient. Chlorella ethanol extract (CE) was used to the comparison as a control. Anticancer activities of the AE and CE were analyzed by HepG2 and HT-29 human cancer cell. The AE showed antimicrobial activities for all tested bacterial strains. Whereas, CE showed antimicrobial activities for several tested bacterial strains only. The CE showed higher total phenolics contents, DPPH and ABTS radical-scavenging activities (47.36 mg/g, 22.42% and 28.58%, respectively) than those of AE (8.88 mg/g, 20.16% and 17.69%, respectively). AE showed anti-diabetic effect on ${\alpha}$-glucosidase inhibitory activity with dose-dependantly manner. The cell viability of AE ($125{\mu}g/mL$) on HepG2 and HT-29 human cancer cells were 38.12% and 11.27%, respectively. It was demonstrated that ethanol was efficient solvent for extracting functional components from A. carterae. These results indicated that AE can be described as a good candidate for using as a functional food ingredient.

Effect of Germanium Treatment on Growth and Production of Organic Germanium in Oplopanax elatus (게르마늄 처리에 따른 땃두릅나무의 생육 증진 효과 및 유기게르마늄 생산)

  • Kim, Hee Young;Seong, Eun Soo;Yoo, Ji Hye;Choi, Jae Hoo;Kang, Byeong Ju;Jeon, Mi Ran;Kim, Myong Jo;Yu, Chang Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.3
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    • pp.214-221
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    • 2016
  • Background: This study was conducted to investigate the effects of germanium treatment on the growth and organic germanium production in the roots of Oplopanax elatus plantlets. Methods and Results: O. elatus plantlets were cultured in Murashige and Skoog (MS) medium with different concentrations of germanium dioxide ($GeO_2$) to analyze optimum growth conditions. Exogenous treatment of $10mg/{\ell}\;GeO_2$ promoted growth and an increase in the contents of chlorophyll a, b and carotenoid in O. elatus. The germanium accumulation and production in roots of O. elatus plantlets treated with organic germanium reached the highest levels. The growth of the aerial and underground portion of O. elatus with organic germanium was greater than that of the control. The accumulation and production of organic germanium reached the highest level ($40.89{\mu}g/plantlet$) with the treatment of $50mg/{\ell}\;GeO_2$. Antioxidant activity measured by DPPH and ABTS assays also increased with the germanium treatment and improved the DPPH and ABTS radical activity by 200% compared with that in the control. In addition, the total phenol and flavonoid contents of the plantlets with a treatment of $50mg/{\ell}\;GeO_2$ were higher than in the control. Conclusions: Taken together, the growth of O. elatus was increased with the treatment of $50mg/{\ell}\;GeO_2$ germanium and the biological references improved, with increased antioxidant activity and organic germanium production.

Biological Activities of Yellow Garlic Extract (황마늘 추출물의 생리활성)

  • Kang, Jae Ran;Hwang, Cho Rong;Sim, Hye Jin;Kang, Min Jung;Kang, Sang Tae;Shin, Jung Hye
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.7
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    • pp.983-992
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    • 2015
  • This study investigated the quality characteristics and biological activities, such as antioxidant, whitening, anti-diabetes, and anti-inflammatory activities, of yellow garlic, by simplify processing time and manufacturing process compared with black garlic. Extracts were prepared various ratios of water and ethanol solvent [water : ethanol (v/v)=100:0, 70:30, 50:50, 30:70, 0:100] from yellow garlic. Alliin content of yellow garlic showed no difference compared with fresh garlic, whereas S-allyl cysteine content of yellow garlic was higher than that of fresh garlic. Alliin content of yellow garlic extracts increased in an ethanol concentration-dependent manner. Total phenol and flavonoid contents were highest in 100% ethanol extract. DPPH and ABTS radical scavenging abilities did not show significant differences among 0~70% ethanol extracts, whereas 100% ethanol extract showed the highest contents of 93.45% and 91.46%, respectively. Tyrosinase and ${\alpha}$-glucosidase inhibitory activities were also highest in 100% ethanol extract, but did not show significant differences among the extract solvents. Water and ethanol extracts from yellow garlic showed anti-inflammatory effects by modulating production of NO and cytokines at a concentration of $100{\mu}g/mL$. We suggest that yellow garlic has antioxidant, whitening, anti-diabetes, and anti-inflammatory activities and can be used as a functional material similar to black garlic.

Antioxidant activity and anti-inflammatory effects of ethanol extract from Allium schoenoprasum (향부추 에탄올 추출물의 항산화 활성 및 항염증 효과)

  • Lim, Sang-ran;Lee, Ji-An
    • Journal of Convergence for Information Technology
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    • v.10 no.7
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    • pp.232-239
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    • 2020
  • This study evaluated the antioxidant and anti-inflammatory activity of ethanol extracts using three parts of the chives plant: the bulb, the leaf, and the flower. As a result of DPPH and ABTS radical scavenging ability, the scavenging activity of the flower extract was higher than that of the bulb and leaf. In addition, as a result of FRAP analysis, antioxidant activity increased in all extracts depending on the extract concentration. The total polyphenol content was high in the following order: flower (11.29±0.37 mgGAE/g) > leaf (6.61±0.14 mgGAE/g) > bulb (5.7±0.67 mgGAE/g) extract. The cytotoxicity of the three extracts against rat macrophage RAW264.7 cells and HaCaT cells, both of which are human cutaneous keratinocyte cell lines, was minimal. NO by LPS was generated as a result of examining the anti-inflammatory activity of each extract through the NO colorimetric analysis method and ELISA. TNF-α secretion was decreased to a significant level in the flower ethanol extract. Therefore, these results indicate that there is a high possibility that the ethanol extract of chives, a natural plant resource, can be used as a cosmetic raw material.

Antioxidant and ACE Inhibitory Activities of Styela clava according to Harvesting Time (채취시기에 따른 미더덕의 부위별 항산화 활성 및 ACE 저해 활성)

  • Lee, Dong-Won;You, Dong-Hyun;Yang, Eun-Kyung;Jang, In-Cheol;Bae, Myung-Suk;Jeon, Yuo-Jin;Kim, Suk-Ju;Lee, Seung-Cheol
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.3
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    • pp.331-336
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    • 2010
  • The antioxidant and angiotensin converting enzyme (ACE) inhibitory harvesting times were evaluated. During February and July of 2008, Styela clava were harvested once per month, and divided into flesh and tunic parts. Each collected part was extracted with water and 70% ethanol. DPPH radical scavenging activity (RSA) for flesh part was higher than that of tunic part, and water extract of flesh harvested at April showed the highest value (53.02% at 10 mg/mL). The highest ABTS RSA was found at water extract of flesh part harvested at March. Water extracts of flesh parts harvested from March to May showed relatively higher ACE inhibitory activity, and freezing did not affect ACE inhibitory activity. The results indicated that antioxidant and ACE inhibitory activity of S. clava were variable depending on harvesting time and parts.

Physicochemical Property and Antioxidative Activity of Hot-Water Extracts from Enzyme Hydrolysate of Astragalus membranaceus (황기 효소분해물 열수추출액의 이화학적 특성 및 항산화 활성)

  • Kwon, Sang-Chul;Choi, Goo-Hee;Hwang, Jong-Hyun;Lee, Kyung-Haeng
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.3
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    • pp.406-413
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    • 2010
  • To enhance the yield and bioactivity of hot-water extract from herbal medicine, Astragalus membranaceus was hydrolyzed with carbohydrases, such as ClariSEB and Fungamyl. After hot-water extracts were prepared from each hydrolysate (HW-C/F), physicochemical property, antioxidant activity and sensory property were evaluated. The solid content ($^{\circ}Brix$) of HW-C/F was higher than hot-water extract from A. membranaceus no treated enzyme (control). Although pH of HW-C/F was lower than that of the control, the acidity was higher. Lightness of Hunter's color values was increased in HW-C/F whereas redness and yellowness were decreased. The contents of reducing sugar, flavonoid and polyphenol of HW-C/F were higher than the control but the content of ascorbic acid was not different from control. The inhibitory activity of HW-C/F against lipid peroxidation was slightly higher than control, but DPPH radical scavenging, ABTS reducing, metal chelating activities were significantly increased by HW-C/F. The sensory evaluation also revealed that the sensory panelists preferred HW-C/F to that of control. Therefore, hydrolysis by carbohydrases for preparation of hot-water extract from A. membranaceus is one of the good methods to improve antioxidative activity and sensory property of hot-water extract.

Antioxidant Compounds and Activities as well as Caffeine Content of Aronia melanocarpa Leaf Tea according to Pan-Roasting Conditions (아로니아잎차의 덖음조건에 따른 항산화 성분과 항산화 활성 및 카페인 함량)

  • Park, Soojin;Jung, Sunghoon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.5
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    • pp.639-645
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    • 2017
  • Differences in bioactive compounds and antioxidant activities of aronia leaf (AL) extracts according to manufacturing conditions such as different number of pan-roasting and different temperatures were investigated. Both total polyphenolic compounds and total flavonoids contents were the highest in six time-pan-roasted AL tea extract ($37.96{\pm}0.48mg$ catechin equivalent/g and $19.96{\pm}0.44mg$ quercetin equivalent/g, respectively) among four tea samples. Antioxidative activities were also the highest in six time-pan-roasted AL tea extract with $IC_{50}$ 0.43 mg/mL and $IC_{50}$ 0.27 mg/mL based on DPPH and ABTS radical scavenging activities, respectively. HPLC analysis revealed that AL tea infusion did not have caffeine regardless of manufacturing conditions, whereas green tea infusion had 3.8 mg/g caffeine. Results demonstrated that AL tea can be expected as caffeine free leaf tea containing antioxidant benefits. Moreover, specific pan-roasting conditions of AL tea would be very important for its functional and sensory attributes.

Antioxidative Activities and Whitening Effects of Ethanol Extract from Crataegus pinnatifida Bunge Fruit (아가위 열매 에탄올 추출물의 항산화 및 미백 효능)

  • Park, Sung-Jin;Kwon, Sung-Pil;Rha, Young-Ah
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.10
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    • pp.1158-1163
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    • 2017
  • This study investigated the antioxidative activity and whitening efficacy of Crataegus pinnatifida Bunge fruit 70% ethanol extract (CFE). The total polyphenol contents of CFE was 61.31 mg/g, and the total flavonoid contents was 25.42 mg/g. The electron donating ability of CFE at a concentration of $1,000{\mu}g/mL$ was 85.80%. The ABTS radical scavenging activity, and reducing power of CFE at a concentration of $1,000{\mu}g/mL$ were 17.3% and 0.31, relatively. The maximum permissible levels of CFE in melanoma cells were $100{\mu}g/mL$. CFE at $50{\mu}g/mL$ reduced melanin contents by 8.5%. CFE at $1,000{\mu}g/mL$ reduced intracellular tyrosinase activity by 46.83%. Collectively, the results of this study suggest that CFE effectively inhibited tyrosinase activity, whereas melanin synthesis was weak. These results suggest that Crataegus pinnatifida Bunge fruit could be used as a whitening agent and antioxidant resources for functional foodstuffs, cosmetics, and beauty industrial materials.