• BMB Reports
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• 제43권11호
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• pp.711-719
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• 2010

Kinomics is an emerging and promising approach for deciphering kinomes. Chemical kinomics is a discipline of chemical genomics that is also referred to as "chemogenomics", which is derived from chemistry and biology. Chemical kinomics has become a powerful approach to decipher complicated phosphorylation-based cellular signaling networks with the aid of small molecules that modulate kinase functions. Moreover, chemical kinomics has played a pivotal role in the field of kinase drug discovery as it enables identification of new molecular targets of small molecule kinase modulators and/or exploitation of novel functions of known kinases and has also provided novel chemical entities as hit/lead compounds. In this short review, contemporary chemical kinomics technologies such as activity-based protein profiling, T7 kinasetagged phages, kinobeads, three-hybrid systems, fluorescenttagged kinase binding assays, and chemical genomic profiling are discussed along with a novel allosteric Bcr-Abl kinase inhibitor (GNF-2/GNF-5) as a successful application of chemical kinomics approaches.

• Wind and Structures
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• 제16권6호
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• pp.629-660
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• 2013

This paper reviews the current state-of-the-art in the numerical evaluation of wind loads on buildings. Important aspects of numerical modeling including (i) turbulence modeling, (ii) inflow boundary conditions, (iii) ground surface roughness, (iv) near wall treatments, and (vi) quantification of wind loads using the techniques of computational fluid dynamics (CFD) are summarized. Relative advantages of Large Eddy Simulation (LES) over Reynolds Averaged Navier-Stokes (RANS) and hybrid RANS-LES over LES are discussed based on physical realism and ease of application for wind load evaluation. Overall LES based simulations seem suitable for wind load evaluation. A need for computational wind load validations in comparison with experimental or field data is emphasized. A comparative study among numerical and experimental wind load evaluation on buildings demonstrated generally good agreements on the mean values, but more work is imperative for accurate peak design wind load evaluations. Particularly more research is needed on transient inlet boundaries and near wall modeling related issues.

• 국제초고층학회논문집
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• 제11권4호
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• pp.331-346
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• 2022

Reproducing the horizontally homogeneous atmospheric boundary layer in computational wind engineering is essential for predicting the wind loads on structures. One of the important issues is to use fully developed inflow conditions, which will lead to the consistence problem between inflow condition and internal roughness. Thus, by analyzing the previous results of computational fluid dynamic modeling turbulent horizontally homogeneous atmospheric boundary layer, we modify the past hypotheses, detailly derive a new type of inflow condition for standard k-ε turbulence model. A group of remedial approaches including formulation for wall shear stress and fixing the values of turbulent kinetic energy and turbulent dissipation rate in first wall adjacent layer cells, are also derived to realize the consistence of inflow condition and internal roughness. By combing the approaches with four different sets of inflow conditions, the well-maintained atmospheric boundary layer flow verifies the feasibility and capability of the proposed inflow conditions and remedial approaches.

• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3349-3355
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• 2012

Background and objectives: Chromosomal abnormalities play an important role in genesis of acute lymphoblastic leukemia (ALL) and have prognostic implications. Five major risk stratifying fusion genes in ALL are BCR-ABL, MLL-AF4, ETV6-RUNX11, E2A-PBX1 and SIL-TAL1. This work aimed to detect common chromosomal translocations and associated fusion oncogenes in adult ALL patients and study their relationship with clinical features and treatment outcome. Methods: We studied fusion oncogenes in 104 adult ALL patients using RT-PCR and interphase-FISH at diagnosis and their association with clinical characteristics and treatment outcome. Results: Five most common fusion genes i.e. BCR-ABL (t 9; 22), TCF3-PBX1 (t 1; 19), ETV6-RUNX1 (t 12; 21), MLL-AF4 (t 4; 11) and SIL-TAL1 (Del 1p32) were found in 82/104 (79%) patients. TCF3-PBX1 fusion gene was associated with lymphadenopathy, SIL-TAL1 positive patients had frequent organomegaly and usually presented with a platelets count of less than $50{\times}10^9/l$. Survival of patients with fusion gene ETV6-RUNX1 was better when compared to patients harboring other genes. MLL-AF4 and BCR-ABL positivity characterized a subset of adult ALL patients with aggressive clinical behaviour and a poor outcome. Conclusions: This is the first study from Pakistan which investigated the frequency of5 fusion oncogenes in adult ALL patients, and their association with clinical features, treatment response and outcome. Frequencies of some of the oncogenes were different from those reported elsewhere and they appear to be associated with distinct clinical characteristics and treatment outcome. This information will help in the prognostic stratification and risk adapted management of adult ALL patients.

• 대한원격탐사학회지
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• 제37권6_1호
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• pp.1709-1718
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• 2021

항공수심라이다[airborne bathymetric LiDAR (Light Detection And Ranging)] 시스템의 웨이브폼(waveform) 데이터는 기존의 이산반환(discrete-return) 데이터에 비해 정확도, 해상도, 신뢰도가 향상된 데이터를 제공하며, 사용자의 데이터 처리 통제력을 강화하며 반환신호에 대한 추가적인 정보 추출을 가능하게 한다. 웨이브폼 분해(waveform decomposition)는 수면과 해저 반사, 수중산란 및 각종 노이즈가 혼재되어 수신된 웨이브폼으로부터 각각의 에코(echo)를 분리하는 기술로, 지형 포인트 추출을 위해 선행되어야 하는 처리 과정이자 측량 성과를 좌우하는 주요 기술이다. 본 연구에서는 항공수심라이다 원시 데이터로부터의 포인트 추출 성능을 향상시키 위해 새로운 웨이브폼 분해 기술을 개발하였다. 기존의 웨이브폼 분해 기술들이 웨이브폼 피크(peak)들을 가우시안(Gaussian) 분해의 초기값으로 사용하여 분해된 에코의 개수와 분해 성능이 피크 탐지 결과에 좌우되는데 반해, 제안한 기술은 최초 피크들 외에 잠재적인 피크 후보들을 추정하여 추가함으로써, 분해 모델의 근사 정확도를 향상시켰다. 국내 개발된 항공수심라이다 장비인 씨호크(Seahawk)로부터 동해안에서 취득된 웨이브폼 데이터를 이용한 실험 결과, 가우시안 분해 방법 대비 제안한 방법의 모델 근사 적합도(RMSE 기준)가 약 37% 향상된 결과를 얻었다.

• 대한기계학회논문집B
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• 제27권1호
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• pp.105-116
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• 2003

The flow structure around the free end of a finite circular cylinder (FC) embedded in an atmospheric boundary layer (ABL) over open terrain was investigated experimentally with varying the free end shape. The experiments were carried out in a closed-return type subsonic wind tunnel. A finite cylinder with an aspect ratio (L/D) of 6 was mounted vertically on a long flat plate. The Reynolds number based on the cylinder diameter is about Re=7,500. The velocity fields near the FC free end were measured using the single-frame double-exposure PIV method. As a result, for the FC with a right-angled free end, there is a peculiar vortical structure, showing counter-rotating twin vortices near the FC free end. It is caused by the interaction between the entrained irrotational fluids from both sides of FC and the downwash flow from the FC free-end.

• Wind and Structures
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• 제30권6호
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• pp.597-616
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• 2020

The blockage effect on the aerodynamic characteristics of tall buildings is a fundamental issue in wind tunnel test but has rarely been addressed. To evaluate the blockage effects on the aerodynamic forces on a square tall building and flow field peripherally, large eddy simulations (LES) were performed on a 3D square cylinder with an aspect ratio of 6:1 under the uniform smooth inflow and turbulent atmospheric boundary layer (ABL) inflow generated by the narrowband synthesis random flow generator (NSRFG). First, a basic case at a blockage ratio (BR) of 0.8% was conducted to validate the adopted numerical methodology. Subsequently, simulations were systematically performed at 6 different BRs. The simulation results were compared in detail to illustrate the differences induced by the blockage, and the mechanism of the blockage effects under turbulent inflow was emphatically analysed. The results reveal that the pressure coefficients, the aerodynamic forces, and the Strouhal number increase monotonically with BRs. Additionally, the increase of BR leads to more coherence of the turbulent structures and the higher intensity of the vortices in the vicinity of the building. Moreover, the blockage effects on the aerodynamic forces and flow field are more significant under smooth inflow than those under turbulent inflow.

• Wind and Structures
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• 제30권1호
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• pp.99-117
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• 2020

This paper focuses on the processes of wind flow in atmospheric boundary layer, to produce realistic full scale pressures for design of low-rise buildings. CFD with LES turbulence closure is implemented on a scale 1:1 prototype building. A proximity study was executed computationally in CFD with LES that suggests new recommendations on the computational domain size, in front of a building model, apart from common RANS-based guidelines (e.g., COST and AIJ). Our findings suggest a location of the test building, different from existing guidelines, and the inflow boundary proximity influences pressure correlation and reproduction of peak loads. The CFD LES results are compared to corresponding pressures from open jet, full scale, wind tunnel, and the ASCE 7-10 standard for roof Component & Cladding design. The CFD LES shows its adequacy to produce peak pressures/loads on buildings, in agreement with field pressures, due to its capabilities of reproducing the spectral contents of the inflow at 1:1 scale.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6653-6656
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• 2013

Background: The single most common proto-oncogene change in human neoplasms is a point mutation in RAS genes. A wide range of variation in frequency of KRAS mutations has been seen in hematologic malignancies. Despite this, RAS roles in leukemogenesis remain unclear. The frequency of KRAS mutations in CML has been reported to be between zero an 10%. Many attempts have been done to develop an anti-RAS drug as a therapeutic target. Materials and Methods: This cross sectional study was performed in Mashhad University of Medical Sciences, Mashhad, Iran from 2010-2012. In 78 CML patients (diagnosed according to WHO 2008 criteria) in chronic or accelerated phases, KRAS mutations in codons 12 and 13 were analyzed using a modified PCR-restriction fragment length polymorphism (RFLP) method. Results: We did not detect any KRAS mutations in this study. Conclusions: KRAS mutations are overall rare in early phase CML and might be secondary events happening late in leukemogenesis cooperating with initial genetic lesions.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2008년도 춘계학술대회논문집
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• pp.705-706
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• 2008

When heat generated inside a large factory building is not discharged due to a stagnant flow, the working environment of workers becomes worse and the cooling of high-temperature products such as hot-rolling coils is delayed. To investigate the natural ventilation inside a large factory building, experimental studies were carried out using wind-tunnel tests. The scale-down factory building models were placed in an atmospheric boundary layer (ABL) and the mean and fluctuating velocity fields were measured using a particle image velocimetry (PIV) technique. For the prototype factory model, the outdoor air is only entrained into the factory building through the one-third open windward wall, and stagnant flow is formed in the rear part of the target area. In order to improve the indoor ventilation environment of the factory building, three different louver-type ventilators were attached at the upper one-third open windward wall of the factory model. Among the three louver ventilators tested in this study, the ventilator model #3 with the outer louver (${\theta}_o$ = 90$^{\circ}$) and the inner louver (${\theta}_i$ = -70$^{\circ}$) was found to improve the natural ventilation inside the factory building model effectively. The flow rate of the entrained air was increased with aligning the outer louver blades with the oncoming wind and guiding the entrained air down to the ground surface with elongated inner louver blades.