• Journal of Microbiology and Biotechnology
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• 제20권6호
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• pp.1042-1052
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• 2010

Forty-seven Salmonella Typhimurium (33 zoonotic, 14 clinical) strains were tested for antimicrobial resistance using the standard disk diffusion method. The presence of relevant resistance genes and class 1 integrons were investigated by using PCR. Pulsed-field gel electrophoresis (PFGE) and plasmid profiling were carried out to determine the genomic diversity of Salmonella Typhimurium. Approximately 57.4% of the S. Typhimurium strains were multidrug resistant (MDR) and showed high resistance rates to tetracycline (70.2%), sulfonamides (57.4%), streptomycin (53.1%), ampicillin (29.7%), nalidixic acid (27.6%), kanamycin (23.4%), chloramphenicol (21.2%), and trimethoprim (19.1%). Resistance towards cephalosporins was noted for cephalothin (27.6%), cephradine (21.2%), amoxicillin clavulanic acid (17.0%), and cephalexin (17.0%). Resistance genes, $bla_{TEM}$, strA, aadA, sul1, sul2, tetA, tetB, and tetC, were detected among the drug-resistant strains. Thirtythree strains (70.2%) carried class 1 integrons, which were grouped in 9 different profiles. DNA sequencing identified sat, aadA, pse-1, and dfrA genes in variable regions on class 1 integrons. Thirty-five strains (74.4%) were subtyped to 22 different plasmid profiles, each with 1-6 plasmids (2.0 to 95 kb). PFGE subtyped the 47 strains into 39 profiles. In conclusion, high rates of multidrug resistance were found among the Malaysian Salmonella Typhimurium strains. The emergence of multidrug-resistant Salmonella Typhimurium to cephalosporin antibiotics was also observed. The strains were very diverse and no persistent clone was observed. The emergence of MDR Salmonella Typhimurium is a worldwide problem, and this report provides information for the better understanding of the prevalence and epidemiology of MDR S. Typhimurium in Malaysia.

• 한국자원식물학회지
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• 제18권3호
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• pp.441-449
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• 2005

광계II(PSII)는 고등식물의 chloroplast에서 두 개의 광합성 반응중심 중의 하나이다. Chlorophyll a/b 광수확 복합체는 광계II를 위한 안테나 역할을 수행한다. 본 연구에서는 인삼의 잎조직을 제작한 cDNA library로부터 chlorophyll a/b-binding protein (Cab) 유전자를 분리하였다. 인삼 Cab유전자는 935 bp의 염기와 265개의 아미노산 잔기(pI 5.63)로 구성된 한 개의 ORF를 포함하고 있으며, 단백질의 분자량은 28.6 kDa으로 추정되었다. 인삼에서 분리한 Cab 유전자는 기존에 식물에서 보고된 유전자들과 유사성을 나타내었으며, 유사도는 $68-92\%$로 나타났다. 아미노산 서열을 비교하여 유연관계를 분석한 결과 인삼의 Cab 유전자는 비교된 P. persica (AAC34983), A.thaliana (AAD28771), G. hirsutum (CAA38025), G. max (AAL29886), V. radiata (AAF89205) 등과 동일한 그룹으로 분리되었다.

• 해양환경안전학회지
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• 제18권1호
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• pp.49-54
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• 2012

본 연구의 목적은 지진발생 시 발생할 수 있는 초장대교량의 관리기준을 기반으로 하여 비상대응절차를 정의하고, 지진의 레벨(Level)별 비상대응 알고리즘을 개발하여 구조물의 센서와 연동하는 교량재난관리시스템인 BDMS(Bridge Disaster Management System) 개발이다. 지금까지의 초장대교량의 지진에 대한 방재시스템은 메뉴얼(Manual) 중심의 방식이며 패쇄적인 시스템을 활용하였으나 본 연구에서는 IT 기술을 접목하고 인터넷 기반의 개방적 시스템을 활용하여 보다 실용적인 시스템으로 개발하였다. 또한 교량관리자별로 업무를 할당하고 그 절차마다 수행해야할 임무를 AAD(Activity Action Diagram)을 통하여 명확하게 규정하였다. 3D 상황판 기능을 제공하여 지진재난 뿐 아니라 다른 자연재난의 중복 발생 시에도 적절한 초기 대응이 가능하도록 설계하였다. 시나리오를 기반으로 비상대응 주체별로 행동요령을 정의 하고 비상대응절차를 구축하여 이를 시스템화한 BDMS을 개발 활용한다면 기존의 경험적, 매뉴얼 중심의 대처방식에서 신속성, 효율성을 가진 지진 재난 방제시스템을 갖게 될 것이다.

• 한국동물위생학회지
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• 제36권2호
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• pp.73-78
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• 2013

This study was conducted to investigate antibiotic resistance among Salmonella Typhimurium isolates from diseased pigs in Gyeongbuk province during the period 1998~2011. One hundred forty one isolates were tested for antibiotic resistance using the standard disk diffusion method and were examined for presence of resistance gene by PCR method. S. Typhimurium showed high drug resistance rates to tetracycline (95.7%), streptomycin (93.6%), ampicillin (86.5%), cephalothin (80.1%), gentamicin (79.4%), and trimethoprim/sulfamethoxazole (72.3%). Resistance gene, blaTEM, blaPSE1, tetA, tetB, tetG, sul1, sul2, aadA, strA, grm, and temA were detected among the antibiotic resistance isolates and temB, tetC, aadB gene were not detected. One hundred twenty one (89.6%) tetA, two (1.5%) tetB and one (0.7%) tetG gene were detected in the 135 tetracycline resistant isolates. Two (1.6%) temA gene were detected in one hundred twenty two ampicillin resistance isolates and temB was not detected.

• 정보처리학회논문지C
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• 제13C권4호
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• pp.461-470
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• 2006

최근, 트랜스포트 계층 이동성 지원 프로토콜로 mSCTP (mobile SCTP)가 제안되었다. mSCTP는 종단 간 이동성 지원은 가능하나 위치관리 메커니즘이 없기 때문에 교신단말이 이동단말일 경우 mSCTP 어소시에이션을 설립하고자 하는 단말(이후, 어소시에이션 설립시작단말이라고 부름)은 외부 망에 위치한 교신단말의 현재위치정보를 알 수 없기 때문에 mSCTP 어소시에이션을 설립할 수 없다. 이러한 문제를 해결하기 위해 어소시에이션 설립시작단말에서 mSCTP와 SIP (Session Initiation Protocol) INVITE 메소드를 연동하여 사용하는 방안이 제안되었으나, 어소시에이션 설립시작단말이 교신단말의 현재위치정보들 획득하는데까지 걸리는 지연시간(이후, 주소획득 지연시간이라 부름)이 길다는 문제가 있다. 이에 본 논문에서는 교신단말의 현재위치정보를 획득하는 것을 목적으로 하는 새로운 SIP 메소드들을 정의하고, 이를 이용하여 주소 획득 지연시간을 최소화하는 연동 방안을 제안한다. 수학적 분석과 시뮬레이션 결과를 통하여 제안하는 방안이 주소획득 지연시간을 감소시킴으로써 모든 유형의 SIP 환경에서 기존의 INVITE 에소드를 사용하는 방안보다 상대적으로 좋은 성능을 보임을 알 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제31권8호
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• pp.1109-1114
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• 2021

Chlamydia pneumoniae is a type of pathogenic gram-negative bacteria that causes various respiratory tract infections including asthma. Chlamydia species infect humans and cause respiratory infection by rupturing the lining of the respiratory which includes the throat, lungs and windpipe. Meanwhile, the function of interleukin-4 (IL-4) in Ch. pneumoniae respiratory infection and its association with the development of airway hyperresponsiveness (AHR) in adulthood and causing allergic airway disease (AAD) are not understood properly. We therefore investigated the role of IL-4 in respiratory infection and allergy caused by early life Chlamydia infection. In this study, Ch. pneumonia strain was propagated and cultured in HEp-2 cells according to standard protocol and infant C57BL/6 mice around 3-4 weeks old were infected to study the role of IL-4 in respiratory infection and allergy caused by early life Chlamydia infection. We observed that IL-4 is linked with Chlamydia respiratory infection and its absence lowers respiratory infection. IL-4R α2 is also responsible for controlling the IL-4 signaling pathway and averts the progression of infection and inflammation. Furthermore, the IL-4 signaling pathway also influences infection-induced AHR and aids in increasing AAD severity. STAT6 also promotes respiratory infection caused by Ch. pneumoniae and further enhanced its downstream process. Our study concluded that IL-4 is a potential target for preventing infection-induced AHR and severe asthma.

• Journal of Plant Biotechnology
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• 제3권1호
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• pp.39-44
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• 2001

Plastid transformation was attempted with soybean [Glycine max (L.) Merr.] leaves and photoautotrophic and embryogenic cultures by particle bombardment using the transforming vector pZVII that carries the coding sequences for both subunits of Chlamydomonas reinhardtii Rubisco and a spectinomycin resistance gene (aadA). Spectinomycin resistant calli were selected from the bombarded leaves but the transgene was not present, indicating that the resistance was due to mutations. The Chlamydomonas rbcL and rbcS genes were shown to be site-specifically integrated into the plastid genome of the embryogenic cells with a very low transformation efficiency. None of the transformed embryogenic lines survived the plant regeneration process so no whole plants were recovered. This result does indicate that it should be possible to insert genes into the plastid genome of the important crop soybean if the overall methods are improved.

• Tribology and Lubricants
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• 제12권4호
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• pp.35-42
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• 1996

The labyrinth seal is one of the widely used non-contact type mechanical seal. Current work was emphasized on the investigation of the air jet effect on the labyrinth seal. To improve the sealing capability of conventional labyrinth seal, air jet was injected against through the leakage flow. In this study, both of the numerical analysis by CFD (Computational Fluid Dynamics) and the experimental measurement were carried out. Both of the turbulence aad the compressible flow model were introduced in CFD analysis. The sealing effect of the leakage clearance and the air jet magnitude were studied in the experiment. The reason of the enhanced sealing was explained as a reduction of effective clearance by jetting air. As a result, the air jet could reduce the effective clearance with a wide range of leakage clearance.

• East Asian Economic Review
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• 제20권1호
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• pp.37-65
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• 2016

This research intends to investigate the progress made in East Asian bond market integration thus far. Price-based measures (AAD indicator and beta-convergence measure), quantity-based measures and econometric techniques (co-integration test, error correction model based Granger causality test) are employed in the analysis. Even though East Asian government bond markets have become more integrated since 2001, the differentials among the markets still remain significantly high. The bond market integration process seems slow. The convergence of bond markets sped up in 2003 and after the 2008 world financial crisis, implying the important role of government policies in integrating the regional bond markets. East Asian bond market integration may need more government-directed measures.

• 한국수소및신에너지학회논문집
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• 제14권4호
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• pp.305-312
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• 2003

A study is presented of the adsorption capacity of a number of different activated carbons for hydrogen at 100 bar aad 298 K. The hydrogen adsorption isotherm was measured by isothermal gravimetric analysis, using a microbalance. The effect of activated carbon's porosity on hydrogen adsorption capacity is surveyed. It is concluded that hydrogen adsorption capacity of activated carbon is lineally increased according to the increase of specific surface area and total pore volume, It seems that microporosity is more contributive than mesoporosity. Most of the adsorbed quantity is due to physical adsorption and chemisorption is negligible, In this work, 0.79 wt.% of hydrogen adsorption capacity is reached.