• International Journal of Oral Biology
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• 제45권4호
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• pp.169-178
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• 2020

L-ascorbic acid (L-AA; vitamin C) induces apoptosis in cancer cells. This study aimed to elucidate the molecular mechanisms of L-AA-induced apoptosis in human laryngeal epidermoid carcinoma Hep-2 cells. L-AA suppressed the viability of Hep-2 cells and induced apoptosis, as shown by the cleavage and condensation of nuclear chromatin and increased number of Annexin V-positive cells. L-AA decreased Bcl-2 protein expression but upregulated Bax protein levels. In addition, cytochrome c release from the mitochondria into the cytosol and activation of caspase-9, -8, and -3 were enhanced by L-AA treatment. Furthermore, apoptosis-inducing factor (AIF) and endonuclease G (EndoG) were translocated into the nucleus during apoptosis of L-AA-treated Hep-2 cells. L-AA effectively inhibited the constitutive nuclear factor-κB (NF-κB) activation and attenuated the nuclear expression of the p65 subunit of NF-κB. Interestingly, L-AA treatment of Hep-2 cells markedly activated Akt and mitogen-activated protein kinase (MAPK; extracellular signal-regulated kinase 1/2, p38, and c-Jun N-terminal kinase [JNK]) and and LY294002 (Akt inhibitor), SB203580 (p38 inhibitor) or SP600125 (a JNK inhibitor) decreased the levels of Annexin V-positive cells. These results suggested that L-AA induces the apoptosis of Hep-2 cells via the nuclear translocation of AIF and EndoG by modulating the Bcl-2 family and MAPK/Akt signaling pathways.

• 한국결정학회지
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• 제14권1호
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• pp.32-34
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• 2003

여러 조건하에서 Clonorchis sinensis로부터 helminth glutatione S-transferase(28kDa isozyme)가 결정화되었다. 0.05 M potassium 완충 용액 (pH 7.0) 내에서 10% polyehtylene glycol MME 550(PEG MME 5 K)에서 자라난 결정이 $3.0{\AA}$ 분해능까지 회절하였다. 이 결정의 결정학적 자료는 다음과 같다 : 단사정계 공간 군 C2, $a=95.83{\AA}$, $b=63.82{\AA}$, $c=235.09{\AA}$, ${\beta}=97.2^{\circ}$.

• 대한기계학회논문집B
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• 제40권10호
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• pp.659-666
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• 2016

본 연구는 길이에 따른 사각 기둥 타입을 변수로 선정하여 각각의 길이 패턴을 지닌 고체 표면에서의 물 액적 젖음 특성을 분자 동역학을 이용하여 규명하였다. 기둥의 표면 형상을 정사각형과 직사각형의 형태로 가정하였고, 정해진 형상에서의 표면 면적을 증가시켰다. 정사각형 형상의 경우 각 변의 길이가 $4.24{\AA}$, $8.48{\AA}$, $12.72{\AA}$로 증가하였으며, 직사각형 형상의 경우 고정된 한 변은 $8.48{\AA}$의 길이를 가지며 다른 한 변의 길이는 $4.24{\AA}$, $8.48{\AA}$, $12.72{\AA}$로 증가하였다. 이러한 길이 변화를 통해 사각기둥 표면형상의 변화 및 전체 고체표면의 면적 대비 기둥이 차지하는 면적의 비율에 따른 물 액적의 변화를 살펴보고, 표면과 물 액적과의 접촉각을 측정하여 비교 분석하였다.

• BMB Reports
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• 제55권4호
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• pp.175-180
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• 2022

Peptides are gaining substantial attention as therapeutics for human diseases. However, they have limitations such as low bioavailability and poor pharmacokinetics. Periostin, a matricellular protein, can stimulate the repair of ischemic tissues by promoting angiogenesis. We have previously reported that a novel angiogenic peptide (amino acids 142-151) is responsible for the pro-angiogenic activity of periostin. To improve the in vivo delivery efficiency of periostin peptide (PP), we used proteins self-assembled into a hollow cage-like structure as a drug delivery nanoplatform in the present study. The periostin peptide was genetically inserted into lumazine synthase (isolated from Aquifex aeolicus) consisting of 60 identical subunits with an icosahedral capsid architecture. The periostin peptide-bearing lumazine synthase protein cage nanoparticle with 60 periostin peptides multivalently displayed was expressed in Escherichia coli and purified to homogeneity. Next, we examined angiogenic activities of this periostin peptide-bearing lumazine synthase protein cage nanoparticle. AaLS-periostin peptide (AaLS-PP), but not AaLS, promoted migration, proliferation, and tube formation of human endothelial colony-forming cells in vitro. Intramuscular injection of PP and AaLS-PP increased blood perfusion and attenuated severe limb loss in the ischemic hindlimb. However, AaLS did not increase blood perfusion or alleviate tissue necrosis. Moreover, in vivo administration of AaLS-PP, but not AaLS, stimulated angiogenesis in the ischemic hindlimb. These results suggest that AaLS is a highly useful nanoplatform for delivering pro-angiogenic peptides such as PP.

• 한국자기학회지
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• 제17권1호
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• pp.22-25
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• 2007

본 연구에서는 하지층으로 Mo을 사용한 스핀밸브구조에서 반강자성체 IrMn의 두께 변화에 따른 자기적 특성을 연구하였다. 사용된 스핀밸브는 Si기판/$SiO_2/Mo(17\;{\AA})NiFe(21\;{\AA})/CoFe(28\;{\AA})/Cu(22\;{\AA})/CoFe(18\;{\AA})/IrMn(t\;{\AA})/Ta(25\;{\AA})$ 구조이다. Mo 박막의 비저항은 $600^{\circ}C$에서 $650^{\circ}C$ 열처리 후 급격히 증가하였다. 반강자성체인 IrMn의 두께 변화(130 ${\AA}$까지)에 따른 자기저항비와 교환결합력을 측정하였다. IrMn의 두께가 65 ${\AA}$ 일때 자기저항비와 교환결합력은 9.65%와 337.5 Oe로 최고값을 나타냈다. 그러나 두께를 더욱 증가시킨 97.5 ${\AA}$ 일때 자기저항비와 교환결합력은 8.2%와 285 Oe로 감소하였으며, IrMn의 두께가 130 ${\AA}$ 일때 자기저항비와 교환결합력은 더욱 감소한 7.65%와 257.5 Oe이었다.

• Steel and Composite Structures
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• 제28권6호
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• pp.759-766
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• 2018

Friction Stir Welding (FSW) is a solid-state process, where the objects are joined together without reaching their melting point. It has been shown that this method is a suitable way to join dissimilar aluminium alloys. The current article employed hole drilling technique to measure the residual stress distribution experimentally in different zones of dissimilar aluminium alloys AA6061-T6 and AA7075-T6 Butt welded using FSW. Results are compared with those of similar AA6061-T6 plates joined using a conventional fusion welding method called tungsten inert gas (TIG). Also, the evolution of the residual stresses in the thickness direction was investigated, and it was found that the maximum residual stresses are below the yield strength of the material in the shoulder region. It was also revealed that the longitudinal residual stresses in the joint were much larger than the transverse residual stresses. Meanwhile, Vickers micro hardness measurements were performed in the cross-section of the samples. The largest hardness values were observed in the stir zone (SZ) adjacent to the advancing side whereas low hardness values were measured at the HAZ of both alloys and the SZ adjacent to the retreating side.

• 반도체디스플레이기술학회지
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• 제15권1호
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• pp.12-16
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• 2016

Toluene-free pressure sensitive adhesives were synthesized by using butyl acrylate (BA), 2-hydroxy ethyl acrylate, methyl methacrylate, acrylic acid (AA) as monomers and ethyl acetate as a solvent. The polymerization recipes were designed by changing 1, 3, 5 part per hundreds monomer (phm) of AA content on the basis of 100 BA parts. Two crosslinking agents, ethyl glycol diglycidyl ether (EDGE) and isophorone diisocyanate (IPDI) were added to the synthesized polymers to increase adhesion due to crosslinking. In the measurement of properties, holding power, peel strength, and initial tackiness increased with AA content due to crosslinking between carboxyl group in AA and epoxy group in EDGE and isocyanate group in IPDI. In the comparison of two crosslinking agents, EDGE showed better in the three properties than IPDI by better reaction of epoxy group of EDGE to carboxyl group of AA.

• Corrosion Science and Technology
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• 제19권6호
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• pp.281-287
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• 2020

Chloride ion is one of the most important corrosive agents in atmospheric corrosion, especially in marine environments. It has high adsorption rate and increases the conductivity of electrolytes. Since chloride ions affect the protective properties and the surface composition of the corrosion product, they increase the corrosion rate. A low level of chloride ions leads to uniform corrosion, whereas a high level of chloride ions may induce localized corrosion. However, higher solution temperatures tend to increase the corrosion rate by enhancing the migration of oxygen in the solution. This work focused on the effect of NaCl concentration and temperature on galvanic corrosion between A516Gr.55 carbon steel and AA7075T6 aluminum alloys. When AA7075T6 aluminum alloy was galvanically coupled to A516Gr.55 carbon steel, AA7075T6 was severely corroded regardless of NaCl concentration and solution temperature, unlike the corrosion properties of single specimen. The combined effect of surface treatment involving carbon steel and aluminum alloy on corrosion behavior was also discussed.

• 대한화학회지
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• 제54권3호
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• pp.291-294
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• 2010

본 연구에서는 다양한 농도의 BCzVBi를 청색 형광도판트, DPVBi를 청색 호스트 물질로 적용한 청색OLED 소자를 제작하였다. 최적화된 고효율 청색 OLED 소자의 적층 구조는 NPB (500 ${\AA}$)/DPVBi:BCzVBi-6%(150 ${\AA}$)/$Alq_3$(300 ${\AA}$)/Liq(20 ${\AA}$)/Al (1000 ${\AA}$)으로 구성되었다. 청색 OLED의 최대휘도는 구동전압 13.8V에서13200 cd/$m^2$이고 전류밀도 및 최대효율은 각각 1000 cd/$m^2$의 휘도에서 26.4 mA/$cm^2$, 구동전압 3.9 V에서 4.24 cd/A 이었다. 도핑된 청색 OLED 소자의 발광효율은 도핑되지 않은 소자의 2배에 이른 반면 색좌표는 (0.16, 0.19)로 서로 비슷하였다. BCzVBi가 6% 도핑된 청색 OLED 445 nm와 470 nm에 2개의 EL 스펙트럼의 Peak이 존재하는 반면 도핑되지 않은 순수한 DPVBi 청색OLED 소자는 456 nm에서의 유일한 Peak만을 보여주고 있다. 이는 호스트 물질인 DPVBi의 LUMO와 도판트 물질인 BCzVBI의 LUMO 사이에 분자 진동에 의한 페르스터 에너지 전이에 기인한 것이다.

• Journal of Preventive Medicine and Public Health
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• 제34권1호
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• pp.73-79
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• 2001

Objectives : To examine predictors of successful control for selfishness, dishonesty, resentment, and fear(SDRF) among Korean Alcoholics Anonymous(AA) members. Methods : This study was a cross-sectional study. The study group included members from 18 different AA groups which were enrolled in the Korean AA Association in 1998. 207 Out of 300 self administered questionnaires were completed by AA members (response rate 69.0%). Alcoholics who attended AA meetings were divided into two groups according to their self reported level of success in SDRF control; one with very successful experience after AA participation and the other with little or no success. In this study, the general characteristics, AA activities, relapse experience, and degree of effort exerted for SDRF control were compared between two groups. In order to study predictors of successful SDRF control after joining AA, 4 logistic regression analyses were performed for each of the 4 SDRF indices. Results : The proportion of those reporting a 'very successful' experience was 19.9% for selfishness, 20.7% for dishonesty, 25.5% for resentment, and 24.7% for fear. After control for the effect of general characteristics, the practice of the 12th step(taking alcoholics to an AA meeting after conveying messages) was found to be a significant predictor for the successful control of both selfishness(OR=6.04) and the dishonesty(OR=7.77). And individuals making every effort for SDRF control showed more successful control of selfishness(OR=4.10), dishonesty(OR=4.01, and fear(OR=34.89). Conclusions : Bivariate and multivariate analyses demonstrated that especially practicing the 12th step and making every effort themselves, may help alcoholics to control SDRF successfully after joining AA.