• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.151-157
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• 2000

This study was carried out to isolate and identify the maysin and related flavonoid analogues in corn silks. Silks were covered with silk bag to prevent pollination and were sampled at 3-5 days after silking. The silks were filled with 100% MeOH and stored at $0^{\circ}C$ until analysis. The MeOH extracts of corn silks were filtered and concentrated at 35-4$0^{\circ}C$. The ${CH}_2$${Cl}_2$ was added on the concentrated aqueous solution to remove the chlorophyll and lipids. The Cis open column (25mm$\times$54 cm) was washed and activated with serial treatment of 500$m\ell$ of 100% MeOH(twice)longrightarrow75% MeOH longrightarrow50% MeOHlongrightarrow30% MeOHlongrightarrow100% $H_2$O(2 times). The concentrated aqueous solution was applied to the $C_{18}$ column and washed with $H_2O$ several times to remove the sugars and water soluble pigments. Neochlorogenic acid, chlorogenic acid and 4-caffeoylquinic acid were eluted with 10% MeOH, and rhamosyl isoorientin was eluted with 30% MeOH, but maysin was eluted with 50% MeOH from the $C_18$ open column. Collected fractions were analyzed with HPLC by using revers-phase Ultras-phere $C_{18}$ column (4.6$\times$250mm, 5$\mu\textrm{m}$) and $H_2$O (10% MeOH containing 0.1% $H_3$${PO}_4$)/MeOH (100% MeOH containing 0.1% H$_3$PO$_4$) linear gradient from 20% to 90% MeOH for 35 minutes, a flow rate of 1 $m\ell$/min and detection at 340nm. The selected fractions were concentrated and applied to the silicic acid column. Maysin was eluted with 500$m\ell$ of 100% ethyl acetate from the silicic acid column for the first purification, and the purity of collected fractions was about 75%, but the purity from the second purification with the Cis column (1/2 $\times$ 43") was greater than 95%. FAB-MS spectral data was obtained with VG7O-VSEQ VG analytical fast atom bombardment mass (UK). $^1$H-NMR and $^{13}$ C-NMR data were obtained with Bruker DPX 400 MHz NMR spectrometers (German) in DMSO-d$_{6}$ at 400 and 100 MHz, respectively.vely.

• Journal of Korean Ophthalmic Optics Society
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• v.9 no.2
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• pp.431-437
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• 2004

This research reviewed the objects of 384 persons (male:86, female:298) from 6 to 50 years old visited the S. Optical shop at Mokpo over two times from Mar. 2002 to Feb. 2004. We obtained the following results by analyzing the visual acuity prescription to keeping at S. Optical shop. 1. The abnormal refraction eyes were 191 persons(49.74%) for simple myopia, 2 persons(0.52%) for myopic simple astigmatism, 180 persons(46.88%) for myopic compound astigmatism, and 1 person(0.26%) for simple hyperopia, 2 persons(0.52%) for hyperopic simple astigmatism, 8 persons(2.08%) for hyperopic compound astigmatism, respectively. 2. Classifying of correction power for 373 persons (male:84, female:289) myopia, so that showed 166 persons(male:36, female) between $$0.25D{\leq_-}2.00D$$, 194 persons(male:46, female:148) between $$2.25D{\leq_-}6.00D$$, 13 persons(male:2, female:11) for over 6.250 respectively. 3. According to the kinds of 192 persons astigmatism subjects(male:53, female:148), direct astigmatism was 145 persons(male:32, female:113), oblique astigmatism 33 persons (male:12, female:21), reverse astigmatism 14 persons(male:5, female:9). 4. The variation of spherical power for myopia showed 299 persons(male:71, female:228) between $$0.00D{\leq_-}0.50D$$, 64 persons(male:11, female:53) between $$0.51D{\leq_-}1.00D$$, 9 persons(male:2, female:7) between $$1.01D{\leq_-}1.50D$$, 1 person(male:0, female:1) between $$1.51D{\leq_-}2.00D$$ variation respectively. Hyperopia showed 8 persons(male:1, female:7) between $$0.00D{\leq_-}0.50D$$, 3 persons(male:1, female:2) between $$0.51D{\leq_-}1.00D$$ variation respectively. 5. The variation of astigmatism power showed 181 persons(male:48 female:113) between $$0.00D{\leq_-}0.25D$$, 25 persons(male:9, female:16) between $$0.26D{\leq_-}0.50D$$, 6 persons(male:0, female:6) between $$0.51D{\leq_-}0.75D$$ astigmatism variation respectively.

• Economic and Environmental Geology
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• v.30 no.5
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• pp.417-431
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• 1997

Characteristics of sedimentary rocks and enrichment of toxic elements in shale and coal from the Chungnam coal field were investigated based upon geochemistry of major, trace and rare earth elements. Shale and coal of the area are interbedded along the Traissic to the Jurassic Daedong Supergroup, which can be subdivided into grey shale, black shale and coal. The coal had been mined, however all the mines are abandonded due to the economic problems. The shale and coal are characterized by relatively low contents of $SiO_2$, and $Al_2O_3$ and high levels of loss-on-ignition (LOI), CaO and $Na_2O$ in comparison with the North American Shale Composite (NASC). Light rare earth elements (La, Ce, Yb and Lu) are highly enriched with the coal. Ratios of $Al_2O_3/Na_2O$ and $K_2O/Na_2O$ in shale and coal range from 30.0 to 351.8 and from 4.2 to 106.8, which have partly negative correlations against $SiO_2/Al_2O_3$ (1.24 to 6.06), respectively. Those are suggested that controls of mineral compositions in shale and coal can be due to substitution and migration of those elements by diagenesis and metamorphism. Shale and coal of the area may be deposited in terrestrial basin deduced from high C/S (39 to 895) and variable composition of organic carbon (0.39 to 18.40 wt.%) and low contents of reduced sulfur (0.01 to 0.05 wt.%). These shale and coal were originated from the high grade metamorphic and/or igneous rocks, and the rare earth elements of those rocks are slightly influenced with diagenesis and metamorphism on the basis of $Al_2O_3$ versus La, La against Ce, Zr versus Yb, the ratios of La/Ce (0.38 to 0.85) and Th/U (3.6 to 14.6). Characteristics of trace and rare earth elements as Co/Th (0.07 to 0.86), La/Sc (0.31 to 11.05), Se/Th (0.28 to 1.06), V/Ni (1.14 to 3.97), Cr/V (1.4 to 28.3), Ni/Co (2.12 to 8.00) and Zr/Hf (22.6~45.1) in the shale and coal argue for inefficient mixing of the simple source lithologies during sedimentation. These rocks also show much variation in $La_N/Yb_N$ (1.36 to 21.68), Th/Yb (3.5 to 20.0) and La/Th (0.31 to 7.89), and their origin is explained by derivation from a mixture of mainly acidic igneous and metamorphic rocks. Average concentrations in the shale and coal are As=7.2 and 7.5, Ba=913 and 974, Cr=500 and 145, Cu=20 and 26, Ni=38 and 35, Pb=30 and 36, and Zn=77 and 92 ppm, respectively, which are similar to those in the NASC. Average enrichment indices for major elements in the shale (0.79) and coal (0.77) are lower than those in the NASC. In addition, average enrichment index for rare earth elements in coal (2.39) is enriched rather than the shale (1.55). On the basis of the NASC, concentrations of minor and/or environmental toxic elements in the shale and coal were depleted of all the elements examined, excepting Cr, Pb, Rb and Th. Average enrichment indices of trace and/or potentially toxic elements (As, Cr, Cu, Ni, Pb, U and Zn) are 1.23 to 1.24 for shale and 1.06 to 1.22 for coal, respectively.

• Journal of Environmental Impact Assessment
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• v.25 no.6
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• pp.414-431
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• 2016

There are numerous closed and abandoned mines in Korea, from which diverse heavy metals (e.g., As, Cd, Cu, Pb, Zn) are released into the surrounding soil, groundwater, surface water, and crops, potentially resulting in detrimental effects on the health of nearby residents. Therefore, we performed human risk assessments of two abandoned metal mines, Yanggok (YG) and Samsanjeil (SJ). The exposure parameters used in this assessment were specific to residents near mines and the included exposure pathways were relevant to areas around metal mines. The computed total excess carcinogenic risks for both areas exceeded the acceptable carcinogenic risk ($1{\times}10^{-6}$), indicating that these areas are likely unsafe due to a carcinogenic hazard. In contrast, the non-carcinogenic risks of the two areas differed among the studied receptors. The hazard indices were higher than the unit risk (=1.0) for male and female adults in YG and male adults in SJ, suggesting that there are non-carcinogenic risks for these groups in the study areas. However, the hazard indices for children in YG and female adults and children in SJ were lower than the unit risk. Consumption of groundwater and crops grown in the area were identified as major exposure pathways for carcinogenic and non-carcinogenic hazards in both areas. Finally, the dominant metals contributing to carcinogenic and non-carcinogenic risks were As and As, Cu, and Pb, respectively. In addition, the carcinogenic and non-carcinogenic risks of YG were evaluated to be 10 and 4 times higher than those of SJ, respectively, resulted from the relatively higher exposure concentration of As in groundwater within SJ area. Because of lacking of several exposure parameters, some of average daily dose (ADD) could not be computed in this study. Furthermore, it is likely that the ADDs of crop-intake pathway included some errors because they were calculated using soil exposure concentrations and bioconcentration factor (BCF) rather than using crop exposure concentrations.

• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.424-431
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• 2017

This study was conducted to set the optimum nutrient solution concentration by growth stage for new strawberry cultivars 'Berrystar' and 'Jukhyang'(Fragaria ${\times}$ ananassa Duch. cvs. 'Berrystar', 'Jukhyang') grown through hydroponics to improve the quality and yield. Three different EC levels were applied to the nutrient solution. The treatment levels were 0.7, 1.0 and 1.3 times higher than the nutrient concentration standard for 'Seolhyang' based on the 'Manual for strawberry cultivation' of Rural Development Administration. Based on the results, there were no significant differences in growth of 'Berrystar' by EC level. 'Jukhyang' showed the most vigorous growth grown in 1.3 times higher nutrient concentration. While the growth of 'Berrystar' and 'Jukhyang' grown in higher EC level has leaves with more chlorophyll concentration. However the quantum yield of leaves was not affected by the treatments. On the treatment with 1.3 times higher EC level, the weight, length, width and firmness of 'Berrystar' and 'Jukhyang' were significantly high. The sugar contents of the harvest analyzed by HPLC did not differed particularly, but the percentage composition of reducing sugar and non-reducing sugar were presented differently depending on the treatments. Marketable fruit yield increased as nutrient concentration increases. However, there were no large differences by treatments. Meanwhile, 'Jukhyang' showed significant difference by nutrient concentration and had the largest yield for a treatment grown in 1.3 times higher EC level. Based on these results, it is recommended to provide the same nutrient solution concentration level to the nutrient concentration standard of 'Seolhyang' for 'Berrystar', and the 1.3 times higher level for 'Jukhyang'.

• Food Science of Animal Resources
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• v.26 no.4
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• pp.431-440
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• 2006

This study was carried out to compare of yield, physico-chemical and sensory characteristics for chicken surimi manufactured by alkaline (pH 11) adjustment with different raw materials. Four experimental groups were surimi with chicken breast (T1) and chicken leg (T2) by spent hen, SF-MDCM (T3) and JY-MDCM (T4). Yield was higher in order of T1>T2>T3>T4 (p<0.05). The yield, physico-chemical and sensory characteristics of T1 were significantly higher than those of other treatments. Especially, $L^*$ and W value, shear force, textural properties, folding test, breaking force, gel strength, breaking $force{\times}deformation$, flavor, color and overall acceptability were higher in T1 but ar value, cooking loss, collagen and myoglobin content of T1 were lower than those of other treatments (p<0.05). Deformation, aroma, juiciness, tenderness were higher but met-myoglobin and yield of T4 were lower than those of T2 and T3 (p<0.05). Crude fat cooking loss and met-myoglobin content were higher in T2 but $b^*$ value, brittleness, hardness, gumminess, chewiness, folding test, breaking $force{\times}deformation$ and aroma of T2 were lower than those of other treatments (p<0.05). pH, collagen and moisture content and br value were higher but crude protein, folding test, $L^*$ and W value, cohesiveness, tenderness of T3 were lower than those of other treatmene (p<0.05). Correlation coefficients (r>0.8) between folding test and other items was positive in crude protein $L^*$ value, shear force and cohesiveness but negative in moisture content (p<0.05).

• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2009.12a
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• pp.287-287
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• 2009

우리나라의 시설재배면적은 51,237ha에 이르며 수경재배 면적을 제외하면 대부분 경운재배를 하고 있는 실정이다. 경운 작업은 대형 농기구를 이용하여 경운하기 때문에 시설내 토양 입자가 단립으로 잘게 부서지며 대형농기구로 인하여 토양이 답압되고 경운층 아래 경반층이 형성되어 뿌리 발육과 배수 불량의 원인을 제공하게 된다. 경운은 기존에 설치되어 있는 관수시설과 지주, 멀칭을 제거한 후 유기물을 투입하고 2~3회 경운작업 후 다시 소형농기구를 이용하여 이랑을 조성한다. 그리고 제거된 관수시설, 멀칭, 지주를 다시 설치하여야 하기 때문에 시설의 경운재배는 가장 고통스러운 악성노동을 수반하게 된다. 따라서 본 시험에서는 시설 고추 유기재배 시 무경운재배 효과에 대하여 검토하고자 전남 나주시 남평읍 평산리 "JD 중동통 미사질양토"에서 시험을 수행하였다. 2009년 3월 11일 "녹광" 고추 품종을 재식 거리 135${\times}$38cm로 정식하여 7월 27일까지 재배한 후, 시험포를 1/2로 나누어 트렉터를 이용하여 경운작업을 실시하고 2009년 8월 1일 억제작형으로 "녹광" 고추 품종을 정식하였다. 그리고 나머지 시험포 1/2은 전작물을 재배한 이랑에 경운작업을 생략하고 전작물 고추의 식물체는 지제부를 절단하여 분해되도록 고랑을 피복하였으며, 고추 포기와 포기 사이에 무경운으로 고추를 정식하여 시험을 수행하였다. 고추 정식 전 7월 30일에 경운 시험포에 10a당 29톤의 물을 관수하였으며, 무경운 시험포에는 10a당 48톤의 물을 관수하고, 관수 2일 후 시험포의 습도와 이랑에 발생되는 균열수를 조사하였다. 이랑의 수분함량은 표토 0.23%, 토양 30cm 깊이의 수분함량은 0.18%로 경운방법에 따라서 차이가 없었으나 토양 20cm 깊이의 수분함량은 경운은 0.25%, 무경운은 0.14%로 경운재배 토양에 비하여 뿌리의 주요 분포지역인 작토층의 수분함량이 44% 정도 낮았다. 그리고 경운재배 이랑에서는 7~35cm 정도 되는 균열이 m당 22개정도 발생되었으나 무경운에서는 관찰되지 않았다. 시설 토양의 생물다양성 지표가 되는 미소동물의 분포를 조사한(9월 16일) 결과 경운재배 토양에서는 보리톡톡기 등 8종 97개체가, 무경운 토양에서는 10종 101개체가 포획되었다. 경운 방법에 따른 시설 유기재배 고추의 수확과수는 주당 139~141과, 수량은 1,367~1,431g/주가 수확되고 10a당 수량은 경운재배가 2,790kg, 무경운재배는 2,666kg이 생산되어 경운방법에 따른 수량 차는 인정되지 않았다. 경운방법에 따른 경영비를 분석한 결과 무경운은 경운재배에 비하여 10a당 유기질 비료비 178천원, 대농구상각비 211천원, 노력비 261천원의 절감으로 경운재배 경영비 4,815천원/10a 대비 약 14%의 경영비가 절감되었다. 그리고 작기마다 반복되는 경운작업과 관수시설, 멀칭, 지주설치 작업을 1회 설치 작업으로 연속사용이 가능하여 시설재배에서 가장 고통스러운 악성노동의 회피가 가능하였다.

• Korean Journal of Food Science and Technology
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• v.42 no.4
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• pp.431-437
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• 2010

This study was carried out to determine the effect of gamma irradiation (3-20 kGy) on the antioxidant, antimicrobial activities and physical characteristics of Sargassum thunbergii (ST) extracts. When ST powder was treated by gamma irradiation, the yields and total phenolic compounds (TPC) of water extracts were increased, but radical scavenging activities were not changed. When ST extract was irradiated, the TPC and DPPH radical scavenging activities were increased. In addition, gamma irradiation of ST extract decreased viscosity and removed color. These results suggest that gamma irradiation would be a useful method for improving the physical characteristics of ST extract while maintaining native biological activities.

• Journal of agricultural medicine and community health
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• v.22 no.1
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• pp.1-18
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• 1997

This study was carried out to reveal the status of health examination among farmers and to attract more attention to the health care system for farmers. Ten pre-trained medical students interviewed the rural residents 18 years of age and older in eight villages which were randomly selected from a county near Taegu city in Korea, in August 1996. Finally 751 persons were interviewed of whom the percentages of male and female were 41.8%, 58.2% respectively. Among the subjects, 361(48.3%) were fully engaged in farming, 184(24.4%) were partly engaged, and the remaining 206(27.3%) were not engaged in farming at all. The overall prevalence of farmer's disease was 23.0% and there was no significant difference between the group of fully engaged in farming(23.3%) and the group of not-fully engaged(22.9%). But the prevalence of farmer's disease in female subjects(27.8%) was significantly higher than that in male(16.2%)(p<0.01). Among the 288 farmer engaged in spraying pesticide, 113(39.2%) had experienced one or more pesticide related symptoms during last one year, but only 18(15.9%) of them had visited medical facilities due to their symptoms. The experience of receiving education about pesticide was significantly correlated with the degree of wearing protectors during pesticide spraying(p<0.001). Among the 736 persons excluding non-respondents, 281(38.2%) received health examination during last one year ; 176(62.6%) of them received free health examination, and 105(37.4%) received charged one. Among the 533 persons 40 years age and older, only 124(23.3%) had received the 'health examination for the elderly' during last one year, which is provided for the 40 years age and older by Korea medical insurance corporation and medical insurance societies. Most of all beneficiaries of self-employed medical insurance thought the imposed contributions as very expensive(77.4%) or moderately expensive(13.2%). The great majority of farmers are exposed to various health risk factors including pesticide, high temperature, overwork etc. comparable to industrial workers. But farmers are excluded from the regular yearly worker's health examination because of not belonging to a company despite they pay relatively more medical insurance contributions compared with the industrial workers and the urban self-employed medical insureds. It is necessary to develop special health management program for farmers such as the special health examination for the industrial workers exposed harmful agents.

• Economic and Environmental Geology
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• v.44 no.5
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• pp.413-431
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• 2011

An Epithemal Au-Ag mineralized zone is developed in the Moisan area of Hwangsan-myeon, Haenam-gun, Jeol-lanam-do, Korea, which is located in the southwestern part of the Ogcheon metamorphic zone. It is hosted in the Hwangsan volcaniclastics of the Haenam Formation of the Late Cretaceous Yucheon Group. This research investigated the characteristics of bedding arrangement, fold, fault, fracture system, quartz vein and the time-relationship of the fracture system to understand the geological structure related to the formation of the mineralized zone. On the basis of this result, the tectonic environment at the time of the mineralization was considered. Beds mainly trend east-northeast and gently dip into north-northwest or south-southeast. Their poles have been rearranged by subhorizontal-upright open fold of (east)-northeast trend as well as dip-slip fault. Fracture system was formed through at least 6~7 different deformation events. D1 event; formation phase of the main fracture set of EW (D1-1) and NS (D1-2) trends with a good extensity, D2 event; that of the extension fracture of NW trend, and conjugate shear fracturing of the EW (dextral) and NS (sinistral) trends, D3 event; that of the extension fracture of NE trend, and conjugate shear refracturing of the EW (sinistral) and NS (dextral) trends, D4 event; that of the extension fracture of NS trend showing a poor extensity, D5 event; that of the extension fracture of NW trend, and conjugate shear refracturing of the EW (dextral) and NS (sinistral) trends, D6 event; that of the extension fracture of EW trend showing a poor extensity. Frequency distribution of fracture sets of each deformation event is D1-1 (19.73 %)> D1-2 (16.44 %)> D3=D5 (14.79 %)> D2 (13.70 %)> D4 (12.33 %)> D6 (8.22 %) in descending order. The average number of fracture sets within 1 meter at each deformation event is D6 (5.00)> D5 = D4 (4.67)> D2 (4.60)> D3 (4.13)> D1-1 (3.33)> D1-2 (2.83) in descending order. The average density of all fractures shows 4.20 fractures/1 m, that is, the average spacing of all fractures is more than 23.8 cm. The frequency distribution of quartz veins at each orientation is as follows: EW (52 %)> NW (28 %)> NS (12 %)> NE (8 %) trends in descending order. The average density of all quartz veins shows 4.14 veins/1 m, that is, the average spacing of all quartz veins is more than 24.2 cm. Microstructural data on the quartz veins indicate that the epithermal Au-Ag mineralization (ca. 77.9~73.1 Ma) in the Moisan area seems to occur mainly along the existing D1 fracture sets of EW and NS trends with a good extensity not under tectonic stress but non-deformational environment directly after epithermal rupture fracturing. The D1 fracturing is considered to occur under the unstable tectonic environment which alternates compression and tension of NS trend due to the oblique northward subduction of the Izanagi plate resulting in the igneous activity and deformation of the Yucheon Group and the Bulguksa igneous rocks during Late Cretaceous time.