Effects of Gamma Irradiation on Antioxidant, Antimicrobial Activities and Physical Characteristics of Sargassum thunbergii Extract

지충이 추출물의 항산화, 항균 활성 및 물리적 특성에 미치는 감마선 조사의 영향

  • Lee, So-Jeong (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Song, Eu-Jin (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Lee, So-Young (Traditional Food Research Group, Korea Food Research Institute) ;
  • Kim, Koth-Bong-Woo-Ri (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Yoon, So-Young (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Lee, Chung-Jo (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Jung, Ji-Yeon (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Park, Na-Bi (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kwak, Ji-Hee (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Park, Jin-Gyu (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jae-Hun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Choi, Jong-Il (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Ju-Woon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Byun, Myung-Woo (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
  • 이소정 (부경대학교 식품공학과/식품연구소) ;
  • 송유진 (부경대학교 식품공학과/식품연구소) ;
  • 이소영 (한국식품연구원 전통식품연구단) ;
  • 김꽃봉우리 (부경대학교 식품공학과/식품연구소) ;
  • 윤소영 (부경대학교 식품공학과/식품연구소) ;
  • 이청조 (부경대학교 식품공학과/식품연구소) ;
  • 정지연 (부경대학교 식품공학과/식품연구소) ;
  • 박나비 (부경대학교 식품공학과/식품연구소) ;
  • 곽지희 (부경대학교 식품공학과/식품연구소) ;
  • 박진규 (한국원자력연구원 방사선 과학연구소) ;
  • 김재훈 (한국원자력연구원 방사선 과학연구소) ;
  • 최종일 (한국원자력연구원 방사선 과학연구소) ;
  • 이주운 (한국원자력연구원 방사선 과학연구소) ;
  • 변명우 (한국원자력연구원 방사선 과학연구소) ;
  • 안동현 (부경대학교 식품공학과/식품연구소)
  • Received : 2010.01.21
  • Accepted : 2010.03.07
  • Published : 2010.08.31

Abstract

This study was carried out to determine the effect of gamma irradiation (3-20 kGy) on the antioxidant, antimicrobial activities and physical characteristics of Sargassum thunbergii (ST) extracts. When ST powder was treated by gamma irradiation, the yields and total phenolic compounds (TPC) of water extracts were increased, but radical scavenging activities were not changed. When ST extract was irradiated, the TPC and DPPH radical scavenging activities were increased. In addition, gamma irradiation of ST extract decreased viscosity and removed color. These results suggest that gamma irradiation would be a useful method for improving the physical characteristics of ST extract while maintaining native biological activities.

지충이(Sargssum thunbergii) 분말 및 에탄올과 물 추출물에 감마선 조사를 실시한 후, 추출물의 항산화, 항균활성 및 물리적 특성 변화를 알아보았다. 그 결과, 지충이 분말에 감마선 조사를 하였을 때, 조사선량에 따라 물 추출물의 수율과 페놀함량이 증가하였으며, DPPH 라디칼 소거능은 차이를 나타내지 않았다. 감마선 조사한 지충이 추출물의 열과 pH 처리에 따른 페놀함량과 DPPH 라디칼 소거능 변화를 측정한 결과, 열 및 pH 처리에 안정한 항산화 활성을 보였다. 감마선 조사에 따른 항균활성 측정결과, 5종의 그람 양성균에 대해 조사선량에 따른 활성의 차이를 보이지 않았다. 한편, 감마선 조사에 따른 색도 및 점도 변화를 알아본 결과, 색도와 점도는 감마선 조사선량의 증가에 따라 유의적으로 감소하였다. 이상의 결과를 통해 감마선 조사는 지충이 추출물의 항산화 및 항균활성 등의 생리활성은 유지시키면서 수율의 증진과 색도 및 점도의 개선을 통해 추출물의 가공적성을 향상시켜 기능성 식품소재로서 적용 가능성을 보여주었다.

Keywords

References

  1. Jung SJ, Lee JH, Song HN, Seong NS, Lee SE, Back NI. Screening for antioxidant activity of plant medicinal extracts. J. Korean Soc. Appl. Biol. Chem. 47: 135-140 (2004)
  2. Cho SH, Park SY, Choi SW. Effects of Eisenia Bicyclis extracts and pill on blood glucose and lipid profile in streptozotocininduced diabetic mice. Korean J. Nutr. 41: 493-501 (2008)
  3. Heo SJ, Jeon YJ. Antioxidant effect and protecting effect against cell damage by enzymatic hydrolysates from marine algae. Food Indus. Nutr. 10: 31-41 (2005)
  4. Lee NH, O KL. Screening of radical scavenging effects from marine algae. Cheju J. Life Sci. 3: 95-101 (2000)
  5. Lee EH, Ham J, Ahn HR, Kim MC, Kim CY, Pan CH, Um BH, Jun SH. Inhibitory effects of the compounds isolated from Sargassum yezoense on $\alpha$-glucosidase and oxidative stress. Korean J. Pharmacogn. 40: 150-154 (2009)
  6. Bae SJ. Anticarcinogenic effects of Sargassum fulvellum fractions on several human cancer cell lines in vitro. J. Korean Soc. Food Sci. Nutr. 33: 480-486 (2004) https://doi.org/10.3746/jkfn.2004.33.3.480
  7. Choi SY, Kim SY, Hur JM, Shin JH, Choi HG, Sung NJ. A study on the physicochemical properties of the Sargassum thunbergii. Korean J. Food Nutr. 19: 8-13 (2006)
  8. Choi SY, Kim SY, Hur JM, Choi HG, Sung NJ. Antioxidant activity of solvent extracts from Sargassum thunbergii. J. Korean Soc. Food Sci. Nutr. 35: 139-144 (2006) https://doi.org/10.3746/jkfn.2006.35.2.139
  9. Lee SY, Song EJ, Kim KBWR, Yoon SY, Kim SJ, Lee SJ, Hong YK, Lim SM, Ahn DH. Antimicrobial activity of ethanol extract from Sargassum thunbergii. J. Korean Soc. Food Sci. Nutr. 38: 502-508 (2009) https://doi.org/10.3746/jkfn.2009.38.4.502
  10. Kang JY, Khan MNA, Park NH, Cho JY, Lee MC, Fujii H, Hong YK. Antipyretic, analgesic, and anti-inflammatory activities of the seaweed Sargassum fulvellum and Sargassum thunbergii in mice. J. Ethnopharm. 116: 187-190 (2008) https://doi.org/10.1016/j.jep.2007.10.032
  11. Park KE, Kim YA, Hyun AJ, Lee HJ, Ahn JW, Lee BJ, Seo YW. Three norisoprenoids from the brown alga Sargassum thunbergii. J. Korean Chem. Soc. 48: 394-398 (2004) https://doi.org/10.5012/jkcs.2004.48.4.394
  12. Yook HS, Cha BS, Jo SK, Byun MW. Effects of gamma irradiation on microbial decontamination, extraction yields, and physiological effectiveness of Korean medicinal plants. Korean J. Food Sci. Technol. 30: 581-589 (1998)
  13. Kim HJ, Jo C, Kim TH, Kim DS, Park MY, Byun MW. Biological evaluation of the methanolic extract of Eriobotrya japonica and its irradiation effect. Korean J. Food Sci. Technol. 38: 684-690 (2006)
  14. Harrison K, Were LM. Effect of gamma irradiation on total phenolic content yield and antioxidant capacity of almond skin extracts. Food Chem. 102: 932-937 (2007) https://doi.org/10.1016/j.foodchem.2006.06.034
  15. Prasad SV, Ashwini L, Arun S. Radiation-induced enhancement of antioxidant contents of soybean (Glycine max Merrill). J. Agr. Food Chem. 52: 3385-3388 (2004) https://doi.org/10.1021/jf030793j
  16. Kim MS, Kim KH, Yook HS. The effect of gamma irradiation on the microbiological, physicochemical, and sensory quality of peach (Prunus persica L. Batsch cv Dangeumdo). J. Korean Soc. Food Sci. Nutr. 38: 364-371 (2009) https://doi.org/10.3746/jkfn.2009.38.3.364
  17. Kim KH, Lee SA, Yook HS. Effects of gamma irradiation on physicochemical properties of red beet and stability of betalain in the red beet (Beta vulgaris L.). J. Korean Soc. Food Sci. Nutr. 36: 453-457 (2007) https://doi.org/10.3746/jkfn.2007.36.4.453
  18. Swain T, Hillis WE. The phenolic constituents of Prunus domestica. I-The quantitative analysis of phenolic constituents. J. Sci. Food Agr. 10: 63-68 (1959) https://doi.org/10.1002/jsfa.2740100110
  19. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1990-2100 (1958)
  20. Lee KJ, Um BH. Extraction of useful component from natural plants using ultrasound system. Korean J. Biotechnol. Bioeng. 23: 101-108 (2008)
  21. Lee NY, Jo C, Sohn SH, Kim JK, Byun MW. Effects of gamma irradiation on the biological activity of green tea byproduct extracts and a comparison with green tea leaf extracts. J. Food Sci. 71: 269-274 (2006) https://doi.org/10.1111/j.1750-3841.2006.00017.x
  22. Huang SJ, Mau JL. Antioxidant properties of methanolic extracts from Agaricus blazei with various doses of $\gamma$-irradiation. LWT-Food Sci. Technol. 39: 707-716 (2006) https://doi.org/10.1016/j.lwt.2005.06.001
  23. Cho YJ, Lee KH, Cha WS, Ju IS, Yun DH, An BJ, Lee SH, Kim MU, Kim JH, Chun SS. Purification and identification of inhibitory compounds from Cheongmoknosang mulberry leaves (Morus alba L.) on Helicobacter pylori. J. Appl. Biol. Chem. 52: 65-69 (2009) https://doi.org/10.3839/jabc.2009.012
  24. Variyar PS, Bandyopadhyay C, Thomas P. Effect of gamma irradiation on the phenolic acids of some Indian spices. Int. J. Food Sci. Technol. 33: 533-537 (1998) https://doi.org/10.1046/j.1365-2621.1998.00219.x
  25. Bae SM, Kim JH, Cho CW, Jeong TJ, Yook HS, Byun MW, Lee SC. Effect of $\gamma$-irradiation on the antioxidant activity of rice hull, rice bran and barley bran. J. Korean Soc. Food Sci. Nutr. 31: 246-250 (2002) https://doi.org/10.3746/jkfn.2002.31.2.246
  26. Kim AR, Song EJ, Kim MJ, Lee SY, Kim KBWR, Kim JH, Kim SJ, Hong YK, Park JG, Kim JH, Lee JW, Byun MW, Ahn DH. Effects of gamma irradiation on antioxidant properties and physical characteristics of Sargassum siliquastrum water extracts. J. Korean Soc. Food Sci. Nutr. 37: 357-361 (2008) https://doi.org/10.3746/jkfn.2008.37.3.357
  27. Song EJ, Lee SY, Kim KBWR, Park JK, Kim JH, Lee JW, Byun MW, Ahn DH. Effect of gamma irradiation on the physical properties of alginic acid and $\lambda$-carrageenan. J. Korean Soc. Food Sci. Nutr. 36: 902-907 (2007) https://doi.org/10.3746/jkfn.2007.36.7.902
  28. Byun MW, Yook HS, Kim KS, Chung CK. Effects of gamma irradiation on physiological effectiveness of Korean medicinal herbs. Radiat. Phys. Chem. 54: 291-300 (1999) https://doi.org/10.1016/S0969-806X(98)00233-3
  29. Monica BP, Natalia LC, Clara AC. Radiation-induced enhancement of antioxidant activity in extracts of rosemary (Rosmarinus officinalis L.). Food Chem. 104: 585-592 (2007) https://doi.org/10.1016/j.foodchem.2006.12.009
  30. Kwon HJ, Jung UH, Park HR, Shin DH, Jo SK. Effects of gamma irradiation on color changes and antioxidative activities of Caesalpinia sappan L. J. Korean Soc. Food Sci. Nutr. 36: 1055-1061 (2007) https://doi.org/10.3746/jkfn.2007.36.8.1055
  31. Lampart-Szczapaa E, Korczakb J, Nogala-Kaluckaa M, Zawirska-Wojtasiakc R. Antioxidant properties of lupin seed products. Food Chem. 83: 279-285 (2003) https://doi.org/10.1016/S0308-8146(03)00091-8
  32. Kim MJ, Song EJ, Kim MJ, Lee SY, Kim KBWR, Kim SJ, Lee SJ, Yoon SY, Kim AR, Jeon YJ, Park JG, Choi JI, Lee JW, Byun MW, Ahn DH. Effect of $\gamma$-irradiation on antioxidant and physicochemical properties of Ishige ikamurai extracts. J. Korean Soc. Food Sci. Nutr. 37: 1485-1490 (2008) https://doi.org/10.3746/jkfn.2008.37.11.1485
  33. Kim HJ, Jo C, Kim HJ, Shin DH, Son JH, Byun MW. Effects of gamma irradiation on color changes and biological activities of ethanol extract of a mechanically pressed juice of bokbunja (Rubus coreanus Miq.). J. Korean Soc. Food Sci. Nutr. 35: 271-277 (2006) https://doi.org/10.3746/jkfn.2006.35.3.271
  34. Kim KS, Lee MH, Cho SH. Radical degradation of sodium alginate. J. Chitin Chitosan 7: 8-13 (2002)
  35. Lee JH, Chang YI, Chang KS. Effect of gamma irradiations on physical properties of buckwheat starch. Food Eng. Prog. 4: 110-119 (2000)
  36. Lee JW. Use of radiation technology for food industry and safety management. Korean Soc. Food Eng. 3: 20-44 (2005)
  37. Jo C, Son JH, Byun MW. Irradiation application for color removal and purification of green tea leaves extracts. Radiat. Phys. Chem. 66: 179-184 (2003) https://doi.org/10.1016/S0969-806X(02)00273-6
  38. Son SH, Jo C, Oh MJ, Sohn CB, Byun MW. Studies on the changes of biological activity and physicochemical characteristics of gamma irradiated dandelion extracts. Food Eng. Prog. 10: 40-47 (2006)