• Title/Summary/Keyword: A4 locus

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Analysis of Journal Locus in a Connecting Rod Bearing (엔진 연결봉 베어링의 운동 궤적 해석)

  • 조명래;정진영;한동철
    • Transactions of the Korean Society of Automotive Engineers
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    • v.6 no.4
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    • pp.17-23
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    • 1998
  • This paper presents the motion of dynamically loaded journal in the connecting rod bearing of reciprocation internal combustion engine. Journal motions in engine bearings have been composed of two components, which was rotational and translational motion. Early study of journal locus in engine bearing had been performed on each motion. This paper has been considered two motions simultaneously. Reynolds equation including the squeeze effect has been analyzed using the ADI method, and real engine bearing and crankshaft system has been considered to calculate the cyclic external force. The equations are performed by 4th order Runge-Kutta method. This paper gives various journal orbits in connecting rod bearing depending on cyclic external forces, rotation speeds, and bearing parameters.

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Studies on the Comparative Analysis of Mating Locus (Y-region) of Schizophyllum commune (치마버섯 Mating Locus(Y-region)의 비교분석에 관한 연구)

  • 이인선;박동철
    • Journal of Life Science
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    • v.12 no.2
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    • pp.173-181
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    • 2002
  • This study was conducted to do the comparative analysis of mating type locus controlling the direct formation of fruiting body in Schizophyllum commune which is indigenous to North America with that of other identified mating locus. The 3120 bp Y-region nucleotide of A $\alpha$ 3 mating locus activating a developmental pathway in S. commune was determined, and appeared to have about 96% homology to S. commune 1-71 $A\alpha$3 allele indigenous to South America, showing strongly a conservative feature. This nucleotide analysis also showed above 96% homology highly in the seven presumed exons, and about 97% in the acidic rich region (AR), about 99% in homeodomain (H7), about 97% in the basic rich region (BR), about 95% in the serine rich region (Ser) respectively. In the comparative analysis to the translated polypeptide sequence, S. commune A $\alpha$ 3 mating locus containing Y-region also showed about 97% homology to the region of S. commune indigenous to North America, but the identity ratio to Y1 including Y4, Y5, Y6 different allele types was declined to about 41~49%. In the analysis of functional loci controlling mating activity, it is assumed to have a highly conservative feature showing about 98% homology in homeodomain polypeptide. Especially, it is notable that the homology ratio of above 85% in homeodomain motif between mating type alleles was higher than in the AR, BR, Ser showing about 10~50% homology.

Depression, Self-esteem, Type A Behavior Pattern, and Locus of Control in Middle School Students (중학생의 우울과 자존감, A형 행동특성, 통제위의 관계)

  • Choi, Mi-Kyoung
    • Korean Journal of Health Education and Promotion
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    • v.28 no.4
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    • pp.51-61
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    • 2011
  • Objectives: The main purpose of this study was to examine factors associated with depression of middle school students in relation to self-esteem, type A behavior pattern, and locus of control. Methods: A survey was administered to a convenience sample of 309 middle school students. The data analysis procedure included frequency distribution, t-test, ANOVA, Pearson correlation coefficients, and stepwise multiple regression using depression as the dependent variable. Results: The prevalence of depression was 27.2%. Stepwise multiple regression revealed that the factors such as self-esteem(${\beta}$=0.422, p<0.001), type A behavior pattern(${\beta}$=0.166, p<0.001), and locus of control(${\beta}$=-0.165, p<0.001) turned out to be significant affecting factors. Forty nine percent of variance in depression was explained by these factors(40% of variance by self-esteem). Conclusions: The findings suggest that personal internalizing variables should be considered when developing mental health education program to prevent the occurrence of depression for middle school students.

Identification and Functional Analysis of Mating Type Loci in the Pleurotus eryngii

  • Ryu, Jae San;Kim, Min-Keun;Park, Bokyung;Ali, Asjad;Joung, Wan-Kyu
    • 한국균학회소식:학술대회논문집
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    • 2015.11a
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    • pp.35-35
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    • 2015
  • Pleurotus eryngii has recently become a major cultivated mushroom; it uses tetrapolar heterothallism as a part of its reproductive process. Sexual development progresses only when the A and B mating types are compatible. Such mating incompatibility occasionally limits the efficiency of breeding programs in which crossing within loci-shared strains or backcrossing strategies are employed. Therefore, understanding the mating system in edible mushroom fungi will help provide a short cut in the development of new strains. We isolated and identified pheromone and receptor genes in the B3 locus of P. eryngii and performed a functional analysis of the genes in the mating process by transformation. A genomic DNA library was constructed to map the entire mating-type locus. The B3 locus was found to contain four pheromone precursor genes and four receptor genes. Remarkably, receptor PESTE3.3.1 has just 34 amino acid residues in its C-terminal cytoplasmic region; therefore, it seems likely to be a receptor-like gene. Real-time quantitative RT-PCR (real-time qRT-PCR) revealed that most pheromone and receptor genes showed significantly higher expression in monokaryotic cells than dikaryotic cells. The pheromone genes PEphb3.1 and PEphb3.3 and the receptor gene PESTE3.3.1 were transformed into P5 (A3B4). The transformants were mated with a tester strain (A4B4), and the progeny showed clamp connections and a normal fruiting body, which indicates the proposed role of these genes in mating and fruiting processes. This result also confirms that PESTE3.3.1 is a receptor gene. In this study, we identified pheromone and receptor genes in the B3 locus of P. eryngii and found that some of those genes appear to play a role in the mating and fruiting processes. These results might help elucidate the mechanism of fruiting differentiation and improve breeding efficiency.

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A Study of Dissatisfaction of Consumers and/or Complaining Behavior Due to Locus of Control (소비자 불만족 및 통제소재에 의한 불평행동에 관한 연구 -냉장고 구매후 행동을 중심으로-)

  • 문숙재
    • Journal of the Korean Home Economics Association
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    • v.26 no.1
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    • pp.137-149
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    • 1988
  • In this study a series of approaches have been made to find out how complaining behavior would show according to the conviction in the locus of control as to where the general contents dissatisfied by consumers and the dissatisfied consumers would place the responsibility for the consequence of behavior and to test the difference of complaining behavior pursuant to demographic variables and the complaining behavior. The results of this study are described as follows: 1. The consumers have largely attributed the locus of responsibility for their dissatisfaction following the purchase of refrigerators to the relevant manufacturers, advertizers, consumer(herself) and distributors, and as for the dissatisfaction by an attribute of refrigerator, a high level of dissatisfaction was revealed on the price and items of properties. 2. There was no difference in the complaining behavior of consumers pursuant to the demographic features. 3. It was revealed that there wee many consumers, among those respondents to questionnaires, who have actually shown their complaing behavior, while most of them would act inthe form o private behavior, because the cases of acting positive way of public behavior, even though they were apt to show complaining behavior, appears to be extremely rare. 4. A significant difference was shown in the complaing behavior pursuant to the conviction in the locus of control.

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DNA Replication is not Required in Re-establishment of HMRE Silencer Function at the HSP82 Yeast Heat Shock Locus

  • Lee, See-Woo;Gross, David S.
    • Journal of Microbiology
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    • v.34 no.1
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    • pp.30-36
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    • 1996
  • We have exmained the re-establishment of HIMRE mediated silencing function on the transcriptional activity of yeast heast shock gene HSP82. To test whether the onset of SIR repression can occur in growing cells in the rpesence of a potent inhibitor of DNA replication, HMRa/HSP82 strains with SIR4- and SIR4S$^{+}$ genetic backgrounds were arrested in S phase by incubation of a culture in 200 mM hydroxyurea for 120 min. It was clear that following a 20 minute heat shock, silencing of the HMRa/HSP82 allele in cells pretreated with hydroxyurea does occur in a SIR4-dependen fashion, even though the kinetics of repression appears to be substantially delayed. We also have tested whether re- establishement of silencing at the HMR/hsp82 locus can occur in G1-arrested cells. Cell cycle arrest at G1 phase was achieved by treatment of early log a cell cultures with .alpha.-factor mating pheromone, which induces G1 arrest. The result suggests that passage through S phase (and therefore DNA replication) is nor required for re-establishing silencer-mediated repression at the HMNRa/HSP82 locus. Finally, to test whether de nono protein synthesis is required for re-establishment of silencer-mediated repression, cells were pretreated with cycloheximide (500 /.mu.g/ml) 120 min. It was apparent that inhibiting protein synthesis delays, but does not prevent, re-establishment of silencer-mediated repression. Altogether, these results indicate that re-establishment of silencer-mediated repression is not dependent on the DNA replication and has no requirement for protein synthesis.s.

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A study on the relationship of family support and personality with quality of life in the Patients receiving radiotherapy for cervix cancer (방사선 치료를 받는 자궁경부암 환자의 가족지지와 성격이 삶의 질에 미치는 영향)

  • Noh, Young-Hee
    • The Journal of Korean Society for Radiation Therapy
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    • v.8 no.1
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    • pp.149-159
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    • 1996
  • This descriptive correlational study was undertaken in order to investigate the relationship of family support and personality with quality of life in patients receiving radiotherapy for cervix cancer and to provide basic data to help them improve a better quality of life. The subjects for this study Were 80 out-patients undergoing a radiation therapy at C university hospital in K-city, from April, 1992 to October, 1994. The data were obtained using a convenience sampling technique. The tools used for this study were Ro's quality of life scale, Kang's family support scale and Wallston & others health locus of control scale. The collected data were analyzed by the SAS program using percentage, mean, standard deviation, pearson's correlation coefficients. The result were as follows : 1. The total average score of the quality of life of the subjects was 138.95(minimum score 121-maximum score 164), item mean score(range 1-5) was 2.95. The total average score of the family support of the subjects was 32.55 (minimum score 16-maximum score 47), item mean score(range 1-5) was 2.95 The total average score of the health locus of control of the subjects was 37.00 (minimum score 24-maximum score 49), item mean score (range 1-6) was 3.36. 2 The results of the analysis of the relationship between the quality of life scale and the health locus of control were as follows : the total average score in the quality of life of internal locus of control scale was 136.97, the total average score in the quality of life of external locus of control scale was 144.90. 3. There was a significant positive correlation between the health locus of control and the quality of life(r = 0.2927, p<0.01). The result of the analysis of the relationship between the each factor in the quality of life and health locus of control were as follows : There were significant differences between the health locus of control and emotional state factor(r=0.1514, p<0.01), economic life factor(r=0.2560, p<0.05), self-esteem factor(r=0.2289, p<0.05), physical state and function factor(r=0.1455, p<0.05), relationship with neighbors factor(r=0.0754, p<0.05), relationship with family factor (r=0.3324, p<0.01). 4. There was a significant positive correlation between the family support and the quality of life(r=0.459, p<0.001). The result of the analysis of the relationship between the each factor in the quality of life and family support were as follows : there were significant differences between family support and emotional state factor (r=0.3891, p<0.01), self-esteem factor(r=0.2661, p<0.05), relationship with family factor (r=0.4353, p<0.001).

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Investigation of gene-gene interactions of clock genes for chronotype in a healthy Korean population

  • Park, Mira;Kim, Soon Ae;Shin, Jieun;Joo, Eun-Jeong
    • Genomics & Informatics
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    • v.18 no.4
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    • pp.38.1-38.9
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    • 2020
  • Chronotype is an important moderator of psychiatric illnesses, which seems to be controlled in some part by genetic factors. Clock genes are the most relevant genes for chronotype. In addition to the roles of individual genes, gene-gene interactions of clock genes substantially contribute to chronotype. We investigated genetic associations and gene-gene interactions of the clock genes BHLHB2, CLOCK, CSNK1E, NR1D1, PER1, PER2, PER3, and TIMELESS for chronotype in 1,293 healthy Korean individuals. Regression analysis was conducted to find associations between single nucleotide polymorphism (SNP) and chronotype. For gene-gene interaction analyses, the quantitative multifactor dimensionality reduction (QMDR) method, a nonparametric model-free method for quantitative phenotypes, were performed. No individual SNP or haplotype showed a significant association with chronotype by both regression analysis and single-locus model of QMDR. QMDR analysis identified NR1D1 rs2314339 and TIMELESS rs4630333 as the best SNP pairs among two-locus interaction models associated with chronotype (cross-validation consistency [CVC] = 8/10, p = 0.041). For the three-locus interaction model, the SNP combination of NR1D1 rs2314339, TIMELESS rs4630333, and PER3 rs228669 showed the best results (CVC = 4/10, p < 0.001). However, because the mean differences between genotype combinations were minor, the clinical roles of clock gene interactions are unlikely to be critical.

Molecular Identification of Cryptosporidium viatorum Infection in a Patient Suffering from Unusual Cryptosporidiosis in West Bengal, India

  • Sardar, Sanjib Kumar;Ghosal, Ajanta;Saito-Nakano, Yumiko;Dutta, Shanta;Nozaki, Tomoyoshi;Ganguly, Sandipan
    • Parasites, Hosts and Diseases
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    • v.59 no.4
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    • pp.409-413
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    • 2021
  • In this study, we have collected and screened a total of 268 stool samples from diarrheal patients admitted to an Infectious disease hospital in Kolkata for the presence of Cryptosporidium spp. The initial diagnosis was carried out by microscopy followed by genus specific polymerase chain reaction assays based on 70 kDa heat shock proteins (HSP70). DNA sequencing of the amplified locus has been employed for determination of genetic diversity of the local isolates. Out of 268 collected samples, 12 (4.48%) were positive for Cryptosporidium spp. Sequences analysis of 70 kDa heat shock proteins locus in 12 Cryptosporidium local isolates revealed that 2.24% and 1.86% of samples were showing 99% to 100% identity with C. parvum and C. hominis. Along with the other 2 major species one recently described globally distributed pathogenic species Cryptosporidium viatorum has been identified. The HSP70 locus sequence of the isolate showed 100% similarity with a previously described isolate of C. viatorum (Accession No. JX978274.1, JX978273.1, and JN846706.1) present in GenBank.

A Relations of Bone Mass Promoting Behaviors for Prevention of Osteoporosis and Multidimensional Health Locus of Control Scale Cluster (골다공증 예방을 위한 골량증진행위와 건강통제위 유형과의 관계)

  • Yeoum, Soon-Gyo
    • Women's Health Nursing
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    • v.3 no.2
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    • pp.208-223
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    • 1997
  • This study was made to suggest the nursing strategies for promoting the behaviors about bone mass health behaviors in order to prevent middle aged women's osteoporosis. This study was a descriptive-correlational design that also concerned to the types which improve bone mass promoting behaviors by inspecting patterns of health locus of control method out of recognizable variables of health improving models influencing on these bone mass promoting behaviors. For these purpose, data were collected by self reported questionnaire in middle school, from 158 women living in Seoul. The measuring tools used in this study about bone mass promoting behaviors and multidimensional health locus of control, were developed by author on the basis of literature review and analyzed by SPSS-PC window, into pearson's correlation, ANOVA, multiple regression, cluster analysis. Data was analyzed as follows. 1. 6 Multidimensional health locus of control scale clusters were existed. : a)cluster I (pure internal), b)cluster II(pure chance), c) cluster III(Believer in control), d), cluster IV(Type VI), e)cluster V(yea sayer), f) cluster VI(nay sayer). There were no findings of the powerful others external cluster and double external cluster. 2. The higher the value of internal health locus of control was, the better the bone mass promoting behaviors were(r=.2891, $p=.00^{**}$). The higher the value of chance external health locus of control was, the worse the bone mass promoting behaviors were(r=-.1367, $p=.00^{**}$). 3. On the basis of these relationships, 6 clusters were significantly different in the bone mass promoting behaviors(F=2.27, $p=.05^*$). The value of bone mass promoting behaviors was ranked the order of type VI>believer in control>pure internal>yea sayer>nay sayer>pure chance external highly. 4. Bone mass promoting behaviors were not significantly different as to age. Suggestion. Based on the results from the study, I would like to make some suggestions as follows. 1) To delay the loss of bone mass in middle aged women, the study on the cluster of the multidimensional health locus of control should be conducted repeatedly. 2) The tool of multidimensional health locus of control should be developed through a qualitative method adjusted on Korean' health culture.

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