• Journal of Acupuncture Research
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• v.26 no.6
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• pp.67-76
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• 2009

Objectives : The purpose of this study is to examine if Radix Aconiti herbal acupuncture may be effective to the neuropathic pain(mechanical allodynia, cold allodynia) in a rat model of neuropathic pain. Methods : To produce the model of neuropathic pain, under isoflurane 2.5% anesthesia, L5 spinal nerve was ligated by 6-0 silk thread. After neurophathic surgery, the author examined if the animals exhibited the behavioral sign of allodynia. The allodynia was assessed by stimulating the medial malleolus with von frey filament and acetone. Three days after the neuropathic surgery, Radix Aconiti herbal acupuncture was injected at Hwando($GB_{30}$) one time per two days for 2 weeks. After that, the author examined the withdrawal response of neuropathic rats' legs by von frey filament and acetone stimulation. And also the author examined c-Fos in the midbrain central gray of neuropathic rats and the change of WBC, RBC, HGB, HCT count in the blood of neuropathic rats. Results : The Radix Aconiti herbal acupuncture injected Hwando($GB_{30}$) decreased the withdrawal response of mechanical allodynia that assessed with von frey filament in RA1-$GB_{30}$, RA2-$GB_{30}$ group as compared with control group. The Radix Aconiti herbal acupuncture injected Hwando($GB_{30}$) decreased the withdrawal response of chemical allodynia(cold allodynia) that assessed with acetone in RA1-$GB_{30}$, RA2-$GB_{30}$ group as compared with control group. The Radix Aconiti herbal acupuncture injected Hwando($GB_{30}$) showed the significant between control group and RA2-$GB_{30}$ group in the c-Fos expression. The Radix Aconiti herbal acupuncture injected Hwando($GB_{30}$) did not show the significant between control group and RA1-$GB_{30}$ group, RA2-$GB_{30}$ group and RA3-$GB_{30}$ group in the WBC, RBC, HGB, HCT count. Conclusions : We have noticed that Radix Aconiti herbal acupuncture at Hwando($GB_{30}$) decreased mechanical allodynia and cold allodynia in the model of neuropathic pain compared with the control group. C-fos expression in the central gray of that group was also decreased compared with the control group. This study can be used as a basic resource on a study and a treatment of pain.

• Biomedical Science Letters
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• v.10 no.2
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• pp.171-177
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• 2004

This study was performed to investigate the effects of liquid culture of Agaricus blazei Murill on the weight gains, food efficiency ratios, serum protein and mineral levels, and serum enzyme activities in growing male rats. Sprague-Dawley rats (7 weeks old) were given four different types of diets for a succeeding period of 6 weeks, respectively: a normal diet group, a control diet group (normal diet + 15％ lard + 0.5％ cholesterol), a 30％ or 40％ A. blazei diet groups (control diet + 30％ or 40％ A. blazei in water) according to the levels of A. blazei supplementation. The body weight gains, food efficiency ratios, and the liver of the rats fed control diet, 30％ or 40％ A. blazei diets were significantly increased compared to rats fed the normal diet, but those of rats fed the 30％ and 40％ A. blazei diets were similar to those of rats fed the control diet. The concentrations of total protein, albumin, glucose, and hematocrit value in serum of rats fed the control diet, 30％ or 40％ A. blazei diets were similar to those of rats fed the normal diet. The concentrations of urea and creatinine in serum of rats fed the 30％ or 40％ A. blazei diets were similar to those of rats fed the control diet. but the urea of rats fed the 30％ and 40％ A. blazei diets were significantly decreased compared to rats fed the normal diet. The concentrations of Ca, P, Fe and Mg, and $Ca^{++}$, $K^+$, $Na^+$ and $Cl^-$ of rats fed the control diet, 30％ or 40％ A. blazei diets were similar to those of rats fed the normal diet. There were no differences in the activities of aspartate aminotransferase, alanine aminotransferase, $\gamma$-glutamyltranspeptidase and alkaline phosphatase in the serum among the experimental groups. In conclusion, the rats fed the A. blazei maintained normal protein and mineral levels, and enzyme activities of serum. But the A. blazei feeding could not decrease the body and liver weights in the rats fed high cholesterol diets.

• Food Science of Animal Resources
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• v.37 no.6
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• pp.884-888
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• 2017

In this study, we report the morphogenetic analysis and genome sequence of a new WCP30 phage of Weissella cibaria, isolated from a fermented food. Based on its morphology, as observed by transmission electron microscopy, WCP30 phage belongs to the family Siphoviridae. Genomic analysis of WCP30 phage showed that it had a 33,697-bp double-stranded DNA genome with 41.2% G+C content. Bioinformatics analysis of the genome revealed 35 open reading frames. A BLASTN search showed that WCP30 phage had low sequence similarity compared to other phages infecting lactic acid bacteria. This is the first report of the morphological features and complete genome sequence of WCP30 phage, which may be useful for controlling the fermentation of dairy foods.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.403-409
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• 2010

Saccharomyces cerevisiae Hsp30 is a plasma membrane heat shock protein that is induced by various environmental stress conditions. However, the functional role of Hsp30 during diverse environmental stressors is not presently known. To gain insight into its function during thermal stress, we have constructed and characterized a ${\Delta}hsp30$ strain during heat stress. $BY4741{\Delta}hsp30$ cells were found to be more sensitive compared with BY4741 cells, when exposed to a lethal heat stress at $50^{\circ}C$. When budding yeast is exposed to either heat shock or weak organic acid, it inhibits Pma1p activity. In this study, we measured the levels of Pma1p in mutant and Wt cells both during optimal temperature and heat shock temperature. We observed that $BY4741{\Delta}hsp30$ cells showed constitutive reduction of Pma1p. To gain further insights into the role of Hsp30 during heat stress, we compared the total protein profile by 2D gel electrophoresis followed by identification of differentially expressed spots by LC-MS. We observed that contrary to that expected from thermal-stress-induced changes in gene expression, the ${\Delta}hsp30$ mutant maintained elevated levels of Pdc1p, Trx1p, and Nbp35p and reduced levels of Atp2p and Sod1p during heat shock. In conclusion, Hsp30 is necessary during lethal heat stress, for the maintenance of Pma1p and a set of thermal stress response functions.

• Korean Journal of Organic Agriculture
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• v.24 no.1
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• pp.99-114
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• 2016

This study was conducted to evaluate effects of various eco light sources with various lighting distance in 'Viking' rose (Rosa spp.) on the growth and flowering quality to be applied for farm sites. Treatment included 10-, 20-, and 30-RL (-BL, -RBL, -FL, and -IL), which referred to red LED (blue LED, red+blue LED, fluorescent, and incandescent) lighting at 10 cm, 20 cm, 30 cm respectively, apart from flowers. NL referred to natural light as a control. Growth and flowering of 'Viking' rose were non-destructively measured at 4, 6, and 8 weeks after treatment (WAT). FL treatment increased plant height at 4, 6, and 8 WAT, regardless of lighting distance, with the shortest height observed for the NL-treated flowers. 30 RL treatment also increased plant height at 6 and 8 WAT. Stem diameter and number of leaves were not significantly different for all the treatments at 8 WAT, with the lowest values observed for RBL treated-flowers among the light source treatments. Number of root was the greatest for the 30 BL-treated flowers (10.0) but the fewest for the 30 FL (4.7). Length of flower neck at 6 WAT was the extended by 6~7 cm in the 10 FL and 20 FL treatments as well as by 5~6 cm in the 20 RL and 30 RL treatments, inducing 100% of flowering. NL increased $a^*$ (29) of flower color, with the lowest value (10) observed for 20 RL. All things considered, 30 RL would be the best interaction treatment of source and distance of eco light to improve plant height and flowering quality of 'Viking' rose.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.5
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• pp.2705-2710
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• 2016

Recent evidence haas indicated that meningioma-associate protein (MAC30) exhibits different expression patterns in various tumors. However, little is known about the value of MAC30 in human squamous cell carcinoma of lung (SQCLC). The purpose of our study was to investigate the expression of MAC30 and to explore its clinical significance in SQCLC patients. A total of 156 Chinese patients diagnosed with SQCLC were selected for this study. The expression of MAC30 in all tissues was confirmed by immunohistochemical staining. Quantitative real-time PCR was performed to analyze MAC30 mRNA expression in 32 cases of SQCLC patients with corresponding non-tumor lung tissues. We observed enhanced mRNA expression of MAC30 in SQCLC as compared to control samples. Further, elevated MAC30 protein expression was strongly associated with poor tumor differentiation, TNM stage, and lymph node metastasis. In addition, we observed that patients with increased MAC30 expression demonstrated poor overall survival. Multivariate analysis explicated that increased MAC30 expression was a valuable independent predictable factor for poor tumor differentiation and short survival in SQCLC patients. Our present study suggests that MAC30 may serve as a biomarker for poor tumor differentiation and outcomes of patients with SQCLC.

• Journal of the Korean Geotechnical Society
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• v.36 no.3
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• pp.15-23
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• 2020

Ground motion models predicting intensity measures on surface use a time-averaged shear wave velocity, V_S30, as a key variable simulating site effect. The V_S30 can be directly estimated from V_S profiles if the profile depth (z) is greater than or equal to 30 m. However, some sites have V_S profiles with z < 30 m. In this case V_S30 can be predicted using extension models. This study proposes new coefficient sets for existing prediction equations using 297 Korea V_S profiles. We have collected V_S profiles from KMA and Geoinfo database. Fitting six existing methods to data, we suggest new coefficients for each method and evaluate their performance. It turns out that if z ≥ 15 m, the standard deviation (σ) of residual in log₁₀ is 0.061, which indicates that the estimated V_S30 is nearly accurate. If z < 15 m, the σ keeps increasing up to 0.1 for z = 5 m, so we caution the use of models at very low z. Nonetheless, we recommend investigating up to 30 m depth for V_S30 calculation if possible.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.05a
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• pp.38-44
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• 2000

To determine the optimum sowing date and nursery period in Alisma piantago in the southern area of Korea, Alisma plantago local cultivar was grown under three different sowing dates and nursery periods. The plant height, leaf width and leaf length at the area sown on the seedbed in June 30 and conducted the growing seedling in 30 days is 12.1cm, 2.5cm and 3.1cm respectively and it shows tess decrease than that of plant height, leaf width and leaf length with 12.3cm, 2.6cm and 3.2cm respectively which was sown on the seedbed in June 20 and carried out the growing seedling in 30 days, and it also shows less decrease than that of plant height, leaf width and leaf length with 15.2cm, 3.1cm and 5.2cm respectively at the area sown on the seedbed in July 10 and condected the growing seedling in the same date. The stem length, number of stems and diameter of root at the area sown on the seedbed in June 30 and conducted the growing seedling in 30 days is 31cm, 11 and 3.7cm respectively and it shows more increase than that of stem length, number of stem and diameter of and on the other hand shows tess decrease than that of stem length, number of stems and diameter of root with 30cm, 10cm, 35cm respectively which was sown on the seedbed in 30 days, and on the other hand shows less decrease than that of stem length, number of stems and diameter of root with 32cm,13cm,3.9cm respectively at the area sown on the seedbed in July 10 and conducted the growing seedling in the same date, The fresh yield of roots at the area sown on the seedbed in June 30 and conducted the growing seedling in 30 days is 431.4Kg/10a and it shows more increase of 26.9Kg than that of fresh yield of root with 410.59g110a which was sown on the seedbed in lune 20an4 carried out the growing seedling in 30 days, and it also shows more increase of 7Kg than that of fresh yield of root with 430.4Kg/10a at the area sown on the seedbed in July 10 and conducted the growing seedling in the same date. The dry yield of roots at the area on the seedbed in June 30 and conducted the growing seedling in 30 days is 351.9Kg110a which was sown on the seedbed in June 20 and carried out the growing seedling in 30 days, and it also shows more increase of 16.4Kg than that of dry yield of root with 335.4Kg110a at the area sown on the seedbed in July 10 and conducted the growing seedling in the same date. Therefore, the seedling period of proper seedbed for high yield bumper crop of Alisma plantago for exporting to Japan is June 30 and the number of days for seedling is 30 days.

• Journal of Korean Society for Atmospheric Environment
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• v.24 no.3
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• pp.357-366
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• 2008

In this study, the analytical performance of trimethylamine (TMA) were investigated with respect to headspace-solid phase microextraction (HS-SPME) method. In order to induce the elution of aqueous TMA to headspace, NaOH was added as a decomposition reagent to aqueous TMA standard. By controlling the combination of three major variables for TMA extraction, the extent of extraction was compared between the two contrasting conditions for each variable (i.e., reaction time (long (L) vs short (S)), exposure temperature (30 vs $50^{\circ}C$), and exposure time (10 vs 30 min)). The results of this comparative analysis showed that the extraction efficiency for all eight types of HS-SPME combinations decreased on the order: L-30-30>L-50-10>L-30-10>L-50-30>S-30-30>S-50-30>S-50-10>S-30-10. The effect of reaction time appeared to exert significant influences on the relative recovery rate of HS-SPME at 90% confidence level. However, the effects of exposure temperature or exposure time were not so significant as reaction time. When the recovery rate of HS-SPME is compared against the direct injection of liquid standard into GC injector, it recorded as 2%. According to this comparative study, the reaction conditions for HS-SPME application can exert significant influences on the analysis of TMA.

• Restorative Dentistry and Endodontics
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• v.6 no.1
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• pp.37-50
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• 1980

Scanning Electron Microscopic (SEM) examination on the labial surface of 91 permanent upper incisors were made after etching procedure with phosphoric acid, sulfuric acid, nitric acid, hydro chloric acid, oxalic acid, formic acid, citric acid and zinc phosphate liquid for 2 minutes. Following results were obtained. 1. In the surfaces etched by 10%. 50% phosphoric acid, 50% sulfuric acid, 10%. 30% nitric acid, 10%. 50% oxalic acid, 10%. 30%. 50% formic acid, 30%. 50% citric acid and zinc phosphate liquid, there appeared to be a preferential removal of prism cores, but in the surfaces etched by 10% phosphoric acid, 50% nitric acid, 10%. 30% hydrochloric acid and 30% oxalic acid, the prism peripheries were removed preferentially. 2. According to Silverstone classification on enamel etching pattern the surface treated by zinc phosphate liquid, 30. 50% citric acid, 10%. 30%. 50% formic acid, 10%. 50% oxalic acid, 10%. 30% nitric acid, 50% sulfuric acid and 10%. 50%. phosphoric acid showed Type 1, and etched by 30% oxalic acid, 10%. 30% hydrochloric acid, 50% nitric acid and 10% phosphoric acid showed Type II. Etching of prism cores was by far the most common occurence. The changes produced could be related to intrinsic differences in histology and / or solubility of enamel.