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Development of a Method for Analyzing the Nicotine Content in Synthetic Flavoring Substances as Unauthorized E-cigarette Liquid by Using HPLC (전자담배 액상 충진제와 유사한 합성착향료 중 니코틴의 HPLC 분석법 개발)

  • Kim, Jae-Young;Lee, Sang-Mok;Chang, Moon-Ik;Cho, Yoon-Jae;Lee, Han-Jin;Chae, Young-Sik;Rhee, Gyu-Sik
    • Korean Journal of Food Science and Technology
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    • v.45 no.6
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    • pp.693-699
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    • 2013
  • A simple, sensitive, and specific method for quantifying the nicotine content of synthetic favoring substances (SFS) was developed using high performance liquid chromatography (HPLC) with a photo-diode array detector (PDA). Nicotine was extracted from SFS samples by using an acid-base liquid-liquid extraction method with dichloromethane and distilled water. The nicotine content was quantified by HPLC/PDA (261.9 nm) with a $C_{18}$ column under a gradient of 10% acetonitrile with 20 mM ammonium formate (ammonia solution adjusted to pH 8.7) to 100% acetonitrile. The calibration curve, analyzed from concentration standards between 0.1 to 2 mg/L, presented linearity with a correlation coefficient ($r^2$)>0.9999. The limit of quantitation (LOQ) of nicotine in SFS was 0.4 mg/kg, and the average recoveries ranged from 76.4% to 96.3%. The repeatability of measurements, expressed as the coefficient of variation (CV%), ranged from 1.74 to 5.12%. This newly developed method for nicotine quantification in SFS can be considered an analytical method with an acceptable level of sensitivity and repeatability.

Chemical Properties of Major Tree Barks in Korea -(I) Chemical Composition of Reducing Sugars and Five Essential Elements- (한국산(韓國産) 주요(主要) 수종(樹種) 수피(樹皮)의 화학적(化學的) 성질(性質) -(I) 일반분석(一般分析), 환원당구성(構成), 무기원소(無機元素)-)

  • Lee, Hwa Hyoung
    • Journal of Korean Society of Forest Science
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    • v.40 no.1
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    • pp.63-69
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    • 1978
  • A bark comprises about 10 to 20 percents of a typical log by volume, and is generally considered as an unwanted residue rather than a potential1y valuable resourses, As the world has been confronted with decreasing forest resources, natural resources pressure dictate that a bark should be a raw material instead of a waste. The utilization of the largely wasted bark of genus Pinus, Quercus, and Populus grown in Korea can be enhanced by learning their chemical properties. However, the chemical study of tree bark grown in korea have never been undertaken. In the present paper, the studies on the chemical properties of bark comprise carbohydrates, lignin, and extractives, composition of reducing sugars and five essential elements. The results may be summarized as follows: 1. Bark are much richer in quantity of lignin and extractives than the corresponding wood, and are chiefly consisted of lignin, extractives and carbohydrates orderly. It is the same with ash contents. Alcohol-benzene extractives of populus bark are the highest among three genus. 2. Although glucose constitutes the major sugar in both pine and hardwoods bark, in pinus, arabinose and xylose are the next but in hard woods, the next is xylose. 3. Essential elements, Ca and Kjeldahl-N are higher in the bark than in the wood. Ca content is the highest among others, and N,K followed it. Essential elements are higher in Quercus than in Populus and pinus.

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Ethnobotanical Study on the Traditional Knowledge of Vascular Plant Resources in South Korea (한국의 관속식물 전통지식에 대한 민속식물학적 연구)

  • Chung, Jae-Min;Kim, Hyun-Jun;Park, Gwang-Woo;Jeong, Hye-Ran;Choi, Kyung;Shin, Chang-Ho
    • Korean Journal of Plant Resources
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    • v.29 no.1
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    • pp.62-89
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    • 2016
  • The great part of the traditional knowledge on ethnobotanical plants and their uses is gradually vanishing due to industrialization, therefore ethnobotanical studies that explore and preserve the knowledge are in urgent needed before the knowledge are lost ever. This study was conducted to record and conserve the traditional knowledge of botanical taxa in Korea from 2006 to 2014. According to the survey results, derived from 17,328 sheets of 1,771 residents at 868 places, the ethnobotanical plants in Korea consisted of a total 924 taxa; 130 families, 493 genera, 813 species, 7 subspecies, 92 varieties and 12 form. Of them, herbs were 619 taxa (67.0%) and woody plants were 305 taxa (33.0%). 707 taxa (76.5%) out of 924 taxa grow wild, 44 taxa (4.8%) were cultivated wild species, 145 taxa (15.7%) were introduced for cultivation, and 28 taxa (3.0%) were naturalized plants. The analysis of usage for 924 taxa showed that the edible use was the highest with 58.9%, followed by medicinal with 26.9%, material with 3.7% and ornamental with 2.3%, respectively. The leaf of plant was the most useful part, followed by stem and root. Quantitative analysis of the ethnobotanical plants in Korea was performed by the basic values of FC, NU and UR, and indices of CI, RFC, RI and CV. As a result, The CI place Artemisia princeps in first position, followed by Aster scaber, Kalopanax septemlobus. The RFC place A. scaber in first position, followed by A. princeps, Aralia elata. The RI place A. princeps in first position, followed by Pinus densiflora, Morus alba. The CV place A. princeps in first position, followed by P. densiflora, K. septemlobus.

The Relation between Mother's LOHAS Propensity and Healthy Dietary Life Attitude perceived by Chinese University Students (대학생 자녀가 지각한 어머니의 로하스(LOHAS) 성향과 건강식생활 태도와의 관련성 연구 - 중국대학생을 중심으로 -)

  • Lee, Yeon-Jung;Ahn, Gee-Jung
    • Culinary science and hospitality research
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    • v.21 no.4
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    • pp.101-120
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    • 2015
  • This study was conducted to examine the relation between a mother's LOHAS lifestyle propensity, healthy dietary life, and nutrient intake perceived by Chinese university students. The subjects consisted of 376 Chinese university students. The research results are summarized below. The mother's LOHAS attitude index was 76.02 points out of a perfect score of 100 points. High LOHAS attitude factors of mothers were 'health', whereas 'social welfare' scored low. The highest LOHAS attitude items of mothers that Chinese university students perceived was 'My mother uses health food, organic food, clean food'(4.26 points). Males who lived on their own in a large city and have normal BMI ($18.5{\sim}22.9kg/m^2$) perceived a high mother's LOHAS score. The highest food habit variables are 'eating without spilling food' 'cleaning up after meals around' and 'washing my hands before meals'. The highest healthy dietary life item is 'I try to maintain a pleasant and enjoyable life'(3.83 points). The highest nutrient intake attitude score is 'I tend to eat yellowish-green vegetables every day.'(3.92 points). According to mother's LOHAS lifestyle score, it was verified that the higher mother's LOHAS lifestyle score, the higher children's food habit, healthy dietary life, and nutrient intake score are. Mother's environment-friendly LOHAS attitude index has a meaningful impact on the child's food habits, healthy dietary life, and nutrient intake.

Evaluation of the Perception and Satisfaction of Working and Internship Abroad -By Undergraduates Studying in Culinary and Foodservice Departments- (해외 취업 및 인턴쉽에 대한 인식과 만족도에 관한 연구 -조리 및 외식관련 전공자를 대상으로-)

  • Choi, Young-Hee;Kim, Il-Soon;Kim, Soo-Yeun
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.2
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    • pp.287-294
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    • 2009
  • This study was conducted to evaluate the perception and satisfaction of undergraduates majoring in culinary arts and food service with working and internship abroad. The responses of the participants to 10 questions regarding perception and 13 questions regarding the importance and satisfaction with working and internship abroad were measured on a 5 point Likert scale. The primary results were as follows : 1) The subjects were composed of 50.9% male and 49.1% female students, of which 42.1% were employed and 57.9% experienced an internship abroad. 2) Most students went abroad to gain experience with respect to various foreign cultures in response to recommendations by the western cuisine department. 3) The items "I wish to conduct my affairs continuously"(M=4.21) and "I have good relationships with my colleagues at work"(M=4.11) received the highest points from male and female respondents, respectively. 4) Male students considered "cooperation among divisions"(M=4.11), "language skills"(M=4.38), and "kitchen environment"(M=4.34) to be very important. However, female students believed that "language skills"(M=4.36),"social relationships"(M=4.21), and "wage income"(M=4.18) were most important. Furthermore, male students were most satisfied with "company size" (M=4.28), "kitchen environment"(M=4.21), and "business hours"(M=4.10), while female students were most satisfied with "kitchen environment","incentive"(M=4.14) and "social relationships"(M=4.11).

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Effects of Hysteroscopic Septotomy on Pregnancy in Patients with History of Infertility or Recurrent Spontaneous Abortion (불임 또는 습관성 유산 환자에서 중격자궁의 치료가 임신에 미치는 영향)

  • Koo, Hwa-Seon;Cha, Sun-Hwa;Yang, Kwang-Moon;Bae, Ju-Youn;Ahn, Hyun-Suk;Han, Ae-Ra;Park, Chan-Woo;Kang, Inn-Soo;Koong, Mi-Kyoung;Lee, Kyung-Sang
    • Clinical and Experimental Reproductive Medicine
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    • v.37 no.4
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    • pp.361-368
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    • 2010
  • Objective: The aim of this study was to evaluate the influences of uterine septum and their elimination on the reproductive outcomes in women who have history of recurrent spontaneous abortion (RSA) and/or infertility. Methods: The medical records of reproductive outcomes in patients who have had history of RSA and infertility who were diagnosed with uterine septum only by hysterosalpingogram (HSG) between January 2008 and December 2009 were retrospectively analyzed. The subjects who have had severe male factor, tubal factors, other uterine factors, endocrine abnormalities, peritoneal factors, and abnormal karyotyping among both partners were excluded. In 27 patients, confirmation of diagnosis by laparoscopy and elimination of uterine septum by trans-vaginal hysteroscopy was done. Seventeen patients were strongly suspected to uterine septum on HSG but tried to get pregnancy without any other procedure for evaluation and management of uterine anomaly. Age matched 42 patients who have history of RSA and/or infertility and diagnosed to normal HSG finding at same period were randomly selected as control. The medical records of reproductive outcomes were analyzed and compared between groups. Results: The mean time of observation after diagnosis was 21.8 months (10 to 32). 55.6% (15/27) of patients in patients who received trans-vaginal hysteroscopic uterine septotomy were success to get pregnancies and was significantly higher than that of 17 patients who did not receive proper management (23.5%, 4/17, p<0.05). In control population, 40.5% (17/42) were success to pregnancies and the differences were not statistically significant compared to both two study groups. The live birth rate which was excluded pregnancy loss by abnormal fetal karyotyping and congenital anomaly were 75% (9/12) in treated septated uterus group and 84.6% (11/13) in control group each which have no statistically significant different. In patients with septated uterus who did not receive proper management showed lower delivery rate (50%, 2/4) than that of other groups but was not statistically significant. Conclusion: According to present data, women with a uterine septum have an increased chance of successful pregnancy with improved obstetric outcome after proper management of the uterine cavity. And these results were showed in patients with no regard to their reproductive history. But, in case of failed to receive proper management, uterine septum can affect not only pregnancy ongoing but successful pregnancy too.

Quality characteristics of rice and rice starch-based Yakju (쌀 및 쌀 전분을 활용한 약주의 품질특성)

  • Kang, Ji-Eun;Kim, Jae-Woon;Choi, Han-Seok;Kim, Chan-Woo;Yeo, Soo-Hwan;Jeong, Seok-Tae
    • Food Science and Preservation
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    • v.22 no.3
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    • pp.353-360
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    • 2015
  • Yakju, a Korean traditional alcoholic beverage, is made from rice and Nuruk. In this study, the fermentation characteristics of Yakju was investigated using rice and rice starch. Ingredients was classified into raw material (rice, rice starch) and starter (enzyme supplements, modified Nuruk, traditional Nuruk, and yellow rice koji) for fermentation. The crude protein content of rice, rice starch, and starter were determined as follows (%): rice 6.69, rice starch 0.44, enzyme supplements 7.84, modified Nuruk 15.29, traditional Nuruk 14.28, and yellow rice koji 7.28. The alcohol content of rice with traditional Nuruk ($20.13{\pm}0.12%$) was higher than other Yakju. The concentration of organic acids of rice starch-based Yakju (389.83~538.34 mg%) was higher than that of rice Yakju (259.27~357.70 mg%). The concentration of nitrogen compound of rice Yakju (498.38~5976.93 ppm) was higher than that of rice starch-based Yakju (600.43~4463.79 ppm). In line with these findings, further studies will be necessary for the quality analysis of the rice, rice starch and fermented starter (enzyme supplements, modified Nuruk, traditional Nuruk and yellow rice koji).

Statistical Study on Respiratory Stability Through RPM Signal Analysis according to Patient Position Under Radiation Therapy and Device (방사선 치료 환자의 자세 및 Device에 따른 RPM 신호 분석을 통한 호흡 안정성의 통계적 고찰)

  • Park, Myung-Hwan;Seo, Jeong-Min;Choi, Byeong-Gi;Shin, Eun-Hyeok;Song, Gi-Won
    • The Journal of Korean Society for Radiation Therapy
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    • v.23 no.2
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    • pp.83-90
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    • 2011
  • Purpose: This study statistically analyzed the difference of the stability of maintaining a respiratory period shown according to position and use of a device to search the tendency and usefulness of a device. Materials and Methods: The study obtained respiratory signals which maintained a respiratory period for 20 minutes each supine and prone position for 11 subjects. The study obtained respiratory signals in a state of using a belly board for 7 patients in a bad condition of a respiratory period in a prone position to analyze a change in respiration and the stability before and after the use of a device. Results: The supine part showed 54.5%, better than the prone part of 36.4% in a case that the stability for maintaining a respiratory period was in a good condition as a fixed respiratory period was well maintained according to the position. 6 patients (85%) showed a maintenance pattern of a respiratory period significantly different before the use and 4 patients showed a significantly good change in the stability for maintaining a respiratory period as a result that belly boards were used for 7 patients that the maintenance of a respiratory period was not in a good condition on a prone position. Conclusion: It seemed that this study could contribute to the maintenance of respiratory period and of respiratory stability as the optimal position for maintenance of respiration and the use of a device such as a belly board were decided through statistic analysis of respiratory signals and its application even if patient position and use of device were decided by the beam arrangement a treatment part of a patient, location of a target, and an expected plan.

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Dressing Effect of Phosphorus Fetilizer on the Growth of Soil Improving Species (비료목생장(肥料木生長)에 미치는 인산비료(燐酸肥料)의 시비효과(施肥效果))

  • Ma, Sang Kyu
    • Journal of Korean Society of Forest Science
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    • v.45 no.1
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    • pp.26-36
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    • 1979
  • Through several trials that has done for making the fertilizing-counter plan on the soil improving species, some results have been got as follows; 1. In the non-phosphorus dressing plots soil improving species have very poor survial ratio and show us to be died step by step. It may be resons that root can not make the nodule in case of non-phosphorus dressing and so tree could not absorb the nitrogen nutrient fixed by the nodule. And root competition with the weedy speces for utilizing the nutrient and oxygen in the soil could be reasons when planting in the heavy weedy rooting site. 2. Triple super phosphate, Fused Mg Phosphate and Fused super phosphate have showed the remarkable effects on the growth of soil improving species within 3rd year after planting. But Koreaan tablet fertilizer(9-12-4) for forest purpose have reacted considerably lower effect in comparision with the above powder and grain type phosphorous fertilizer. 3. In case of tablet type fertilizer tree root will have very little phosphorus absorbing surface because phosphorus can be utilized only from the tablet surface and root can not penetrate into the tablet. This my be reson to show the poor dressing reaction of tablet fertilizer but tablet fertilizer has a possibility to be utilized during long years as a sympton in photo 6. So tablet fertilizer can have a recommendation to dress much fertilizer at p]anting year and then tree root can get much more chance for absorbing the phosphorus that could keep the high survival and for utilizing it during many years without after dressing. 4. The granurar and powder type phosphate can develop the dense root mat like photo 8 because of giving the large surface for absorbing the phosphorus and weedy root can approch to the nodule for taking the nitrogen element. So this type seems to present better effect than tablet type to control the soil movement, stem weight as 200g per meter(l meter long${\times}$0.1m width). When added the lime any effect could not be found and rather give the negative effect. So Lespedeza seed sowing and phosphorus dressing system seems us to be very reasonable method for covering the raw material of basket making, fodder and fuel wood supply. 7. Fused Mg phosphate and Fused super phosphate are good fertilizer to the soil improving species and dressing more than 30g per seedling can be recommendable amount. 5. In the unproductive and dry soil with phosphorus fertilizer Robinia pseudoacacia and Alnus firnui can grow more than 2.3m in height at 3rd year and Alnus inokumae have the rapid height growth that is more than 1.8m at 2nd year. Depending on the growth situation like the above example minirotated management has possibilities and rapid covering of erosed land can be done with the soil improving species and phosphorus fertilizer. 6. In the Lespedeza sowing plot with 40g Fused Mg phosphate dressing per meter in the eroded and unproductive forest soil Lespedeza have completely covered this poor land and produced the green.

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A Study on the Utilzation of Two Furrow Combine (2조형(條型) Combine의 이용(利用)에 관(關)한 연구(硏究))

  • Lee, Sang Woo;Kim, Soung Rai
    • Korean Journal of Agricultural Science
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    • v.3 no.1
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    • pp.95-104
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    • 1976
  • This study was conducted to test the harvesting operation of two kinds of rice varieties such as Milyang #15 and Tong-il with a imported two furrow Japanese combine and was performed to find out the operational accuracy of it, the adaptability of this machine, and the feasibility of supplying this machine to rural area in Korea. The results obtained in this study are summarized as follows; 1. The harvesting test of the Milyang #15 was carried out 5 times from the optimum harvesting operation was good regardless of its maturity. The field grain loss ratio and the rate of unthreshed paddy were all about 1 percent. 2. The field grain loss of Tong-il harvested was increased from 5.13% to 10.34% along its maturity as shown in Fig 1. In considering this, it was needed that the combine mechanism should be improved mechanically for harvesting of Tong-il rice variety. 3. The rate of unthreshed paddy of Tong-il rice variety of which stem was short was average 1.6 percent, because the sample combine used in this study was developed on basisof the long stem variety in Japan, therefore some ears owing to the uneven stem of Tong-il rice could nat reach the teeth of the threshing drum. 4. The cracking rates of brown rice depending mostly upon the revolution speed of the threshing drum(240-350 rpm) in harvesting of Tong-il and Milyang #15 were all below 1 percent, and there was no significance between two varieties. 5. Since the ears of Tong-il rice variety covered with its leaves, a lots of trashes was produced, especially when threshed in raw materials, and the cleaning and the trashout mechanisms were clogged with those trashes very often, and so these two mechanisms were needed for being improved. 6. The sample combine of which track pressure was $0.19kg/cm^2$ could drive on the soft ground of which sinking was even 25cm as shown in Fig 3. But in considering the reaping height adjustment, 5cm sinking may be afford to drive the combine on the irregular sinking level ground without any readjustment of the resaping height. 7. The harvesting expenses per ha. by the sample combine of which annual coverage area is 4.7 ha. under conditions that the yearly workable days is 40, percentage of days being good for harvesting operation is 60%, field efficiency is 56%, working speed is 0.273m/sec, and daily workable hours is 8 hrs is reasonable to spread this combine to rural area in Korea, comparing to the expenses by the conventional harvesting expenses, if mechanical improvement is supplemented so as to harvest Tong-il rice. 8. In order to harvest Tong-il rice, the two furrow combine should be needed some mechanical improvements that divider can control not to touch ears of paddy, the space between the feeding chain and the thrshing drum is reduced, trash treatment apparatus must be improved, fore and rear adjust-interval is enlarged, and width of track must be enlarged so as to drive on the soft ground.

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