• Title/Summary/Keyword: A20

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Changes in Ovarian and Placental 20α-hydroxysteroid Dehydrogenase Activity during the Pregnancy in the Rat

  • Seong, H.H.;Min, K.S.;Kang, M.H.;Yoon, J.T.;Jin, H.J.;Chung, H.J.;Chang, W.K.;Yun, S.G.;Shiota, Kunio
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.3
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    • pp.342-347
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    • 2003
  • The enzyme $20{\alpha}$-hydroxysteroid dehydrogenase ($20{\alpha}$-HSD) catabolizes progesterone to $20{\alpha}$-dihydroprogesterone ($20{\alpha}$-OHP), and is appeared in rat corpora luteal and placenta. A polled samples of 10-15 placental and ovarian tissues collected from each individual rat were subjected to measurement of $20{\alpha}$-HSD activity. A $20{\alpha}$-HSD activity in the cytosol fraction was based on the generation of NADPH. In this study, it is designed to study cytosolic $20{\alpha}$-HSD activity in rat ovarian and placenta during pregnancy, and its relationship to embryonic mortality. It was found that from days 5 to 18 of pregnancy the $20{\alpha}$-HSD activities steady by decreased but at parturition time rapidly increased in ovary. On the other hand, placental cytosolic $20{\alpha}$-HSD activities were high detected from days 8 to 10 of pregnancy, not detectable from days 11 to 20 of pregnancy, but again very high at the time of parturition. Analysis of DEAE column chromatography revealed that two different types of $20{\alpha}$-HSD (HSD-1 and HSD-2) were found with similar activity in the placental cytosol on day 10 of pregnancy. The number of fetuses on day 10 of pregnancy was 15.4 and decreased significantly to 12.9 on day 12. The results suggested that expression of $20{\alpha}$-HSD in the placental tissues seems to be related the number of fetal survived in the specific time (days 11 and 12) which spontaneous fetal loss occurs.

An Experiment Study on the Combustion Characteristics with BD20 according to Ultrasonic Energy Irradiation Duration and Injection Delay in a Diesel Engine (초음파 에너지 조사 시간과 분사지연에 따른 BD20의 디젤기관 연소특성에 관한 실험 연구)

  • Im, Seok-Yeon;Lee, Ho-Gil;Ryu, Jeong-In
    • Transactions of the Korean Society of Automotive Engineers
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    • v.16 no.4
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    • pp.38-46
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    • 2008
  • An object of this study is to understand the correlation between the characteristics of an engine performance and combustion characteristics, applying BD20 fuel reformed by ultrasonic energy irradiation to diesel engines. Before conducting the main experiment, an experiment was performed to determine the optimum injection timimg of reformed BD20 by ultrasonic energy irradiation. To control the duration of the ultrasonic energy irradiation, the capacity of an ultrasonic energy fuel supply system was tested with 550cc and 1100cc chambers. As the result of the analysis of the regular BD20 and reformed BD20 by ultrasonic energy irradiation, the BSFC and the Power of the reformed BD20 was improved 3% and 6%, respectively compared to those of non-irradiated BD20. When the fuel injection timing was delayed by $5^{\circ}$, the engine power was improved by 3%, and the BSFC was improved by 2%. The maximum cylinder pressure of reformed BD20 was improved by a maximum of 6% in comparison to that of regular BD20, and demonstrated a synergistic effect of 3% by delaying the injection timing $5^{\circ}$.

Cross-resistance of Colletotrichum acutatum s. lat. to Strobilurin Fungicides and Inhibitory Effect of Fungicides with Other Mechanisms on C. acutatum s. lat. Resistant to Pyraclostrobin (Strobilurin계 살균제에 대한 고추탄저병균의 교차저항성과 Pyraclostrobin 저항성균에 대한 다른 기작 살균제의 억제 효과)

  • Park, Subin;Kim, Heung Tae
    • Research in Plant Disease
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    • v.28 no.3
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    • pp.122-131
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    • 2022
  • Colletotrichum acutatum s. lat. 20JDS8 sensitive and 20CDJ6 resistant to pylaclostrobin were used to investigate the cross-resistance with fungicides belonging to the strobilurins and the characteristics of fungicidal controlling activities with different mechanisms against the isolate resistant to the fungicide. The resistant isolate of 20CDJ6 also showed the resistance to azoxystrobin, trifloxystrobin, and kresoxim-methyl, suggesting that there is a cross-resistance relationship. All fungicides with different action mechanisms inhibited mycelial growth of both susceptible and resistant isolates of C. acutatum s. lat., but their disease control effects in fruits were different according to the fungicides. The disease control effect of isopyrazam against 20JDS8 and 20CDJ6 was very low, and fluazinam showed a control effect of 91.9% and 88.1% against 20JDS8 and 20CDJ6 only when it was treated before inoculation by spraying spore suspensions on pepper fruits without wounds. Tebuconazole and prochloraz effectively inhibited not only the mycelial growth of 20JDS8 and 20CDJ6 on potato dextrose agar medium, but also disease incidence in red pepper fruits. As a result of this study, C. acutatum s. lat. 20CDJ6 resistant to pyraclostrobin showed cross-resistance with other strobilurin fungicides. In addition, we think that fluazinam, tebuconazole, and prochloraz can be recommended as alternative fungicides for the control of red-pepper pyranthracnose pathogens resistant pyraclostrobin. However, fluazinam can be effective only if it is treated protectively before the occurrence of the disease.

Administration Route Dependency of Distribution of Distribution pf PEGylated Recombinant Human Tumor Necrosis Factor Binding Protein (rhTNFbp-PEG20K dimer) following i.v. and s.c. Injection

  • Kim, Dong-Chool;Duane C. Bloedow
    • Archives of Pharmacal Research
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    • v.17 no.5
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    • pp.381-382
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    • 1994
  • Administration route dependency on the distribution of PEGylated recombinant human turor necrosis factor binding protein (rhTNFbp-PEG20K dimer) was observed following a subcutaneous (sc) and an intravenous (iv) administrationin rats. ehTNFbp-PEG20K dimer is composed of two rhTNGbp molecules (molecular weight 18, 278 daltons each) joined by polyethylene glycol 2000(PEG30K). The steady state distribution volume of rhTNFbp-PEG20K was 55 m/kg and 359 ml/kg following the i.v. and s.c. administrations, respectively. These results suggest that the distribution of ehTNFbp-PEG20K is limited within the cpillary space after i.v. administration, while rhTNFbp-PEG20K can distribute into a space (35.9% of body weight) which is between extracellylar space and total body water. A lymphatic absorption may paly a role in the distribution of rhTNFbp-PEF20K dimer following the sc administration. The present study suggests that the administration route of a lartge protein molecule should be determined depedning upon target sites.

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Effect of Bacillus mesonae H20-5 on Fruit Yields and Quality in Protected Cultivation

  • Yoo, Sung-Je;Kim, Jeong Woong;Kim, Sang Tae;Weon, Hang-Yeon;Song, Jaekyeong;Sang, Mee Kyung
    • Research in Plant Disease
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    • v.25 no.2
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    • pp.84-88
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    • 2019
  • A variety of microorganisms in rhizosphere affect plant health by plant growth promotion, mitigation of abiotic stresses as well as protection from pathogen attacks. In our previous study, we selected a bacterium, Bacillus mesonae H20-5, for alleviation of salinity stress in tomato plants. In this study, we verified the effect of a liquid formulation of B. mesonae H20-5 (TP-H20-5) on fruit production and phytochemical accumulation including lycopene and polyphenol in cherry tomato and strawberry fruits in on-farm tests of protected cultivation under salinity stress. When vegetables including tomato, cherry tomato, strawberry, and cucumber were treated with TP-H20-5 by irrigated systems, final marketable yields were increased by 21.4% (cherry tomato), 9.3% (ripen tomato), 120.6% (strawberry), and 14.5% (cucumber) compared to untreated control. Moreover, treatment of TP-H20-5 was showed increase of phytochemicals such as lycopene and total polyphenol compared to untreated control in cherry tomato and strawberry. Therefore, these results indicated that a formulant of B. mesonae H20-5 can be used as a potential biofertilizer for increasing fruit production and quality.

Adult Males' Shoulder and Arm Types for Fitted Sleeve Development - Focusing on Body Mass Index -

  • Su-Joung Cha
    • Journal of the Korea Society of Computer and Information
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    • v.29 no.8
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    • pp.133-145
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    • 2024
  • This study attempted to typify the shoulder and arm morphology of adult males by analyzing the shoulder and arm-related items by obesity level for adult males aged 20 to 69 years. I also examined the typified shoulder and arm characteristics and attempted to analyze the types by obesity level for each age group. The shoulders and arms of adult males were categorized into four types: broad, drooping shoulders with thin, long arms; narrow, drooping shoulders with thick, long arms; broad, rising shoulders with short, thick arms; and narrow, rising shoulders with short, thin arms. The underweight group had a higher percentage of Type 1 in their 20s, 30s, and 60s, and a higher percentage of Type 4 in their 40s. The normal weight group was analyzed as having a higher percentage of type 1 in their 20s and 30s, and a higher percentage of type 4 in their 40s, 50s, and 60s. Obese group was analyzed as having a higher percentage of type 3 in their 20s, 30s, 40s, and a higher percentage of type 4 in their 50s and 60s.

Effect of Daily Protein Allowances during Lay on Egg Production and Feed Cost of Broiler Breeders (육용종계 산란기의 단백질 공급수준이 산란능력과 경제성에 미치는 영향)

  • 함영훈;김상인;이규호
    • Korean Journal of Poultry Science
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    • v.30 no.2
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    • pp.101-106
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    • 2003
  • In order to study the optimum daily protein allowance for broiler breeders of 24 to 64 weeks of age on a control-fed basis, an experiment was carried out with 400 hens of Arbor Acres strain. Four levels of protein allowances were used to supply 18 to 24g of protein per day in 2g increments. As the age of hens increased, a same stepwise increase and decrease in daily energy allotment was used in all treatments. Same amount of calcium, phosphorus, methionine and lysine were supplied in all treatments and throughout laying period. Hen-day egg production was highest in hens receiving 20g protein per day(p<0.05), but there was no significant difference among those fed daily protein ranging 18 to 22g, and increasing the daily protein allotment up to 24g resulted in a significant decrease(p<0.05). Average egg weight showed a trend to increase as the daily protein allowance increase(p<0.05), but no significant difference was found among the hens fed daily protein 20 to 24g. Feed and ME conversion was superior in hens receiving 20g daily protein(p<0.05). CP conversion was increased as the daily protein allowance increase(p<0.05), but there was no significant difference between hens receiving 18 and 20g daily protein. Feed cost required per egg or per kg egg was lowest in hens fed 20g daily protein. It could be concluded that the optimum daily protein allowance was 20g in all performances.

Separation and Purification of Teicoplanin by Diaion HP-20 and Conacnavalin A Chromatography (소수성 Diaion HP-20및 친화성 Concannvalin A 크로마토그래피를 이용한 Glycopeptide계 항생제 Teicoplanin의 분리 및 정제)

  • 김창진;이재찬;박해룡;박동진;김영배
    • Microbiology and Biotechnology Letters
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    • v.31 no.2
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    • pp.201-204
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    • 2003
  • Glycopeptide antibiotics, teicoplanin was purified from a mutant strain of Actinoplanes teichomyceticus ATCC31121, A. teichomyceticus MSL2211. We developed a simple procedure to separate and purify the teicoplanin from the fermentation broth. Teicoplanin was purified by two-step purification system, hydrophobic adsorption and sugar affinity chromatography in combination with HPLC analysis based on the properties of hydrophobic acyl chain and sugar moiety in teicoplanin. Teicoplanin was separated from the culture broth by Diaion HP-20 and further purified by concanavalin A affinity column chromatography. As an adsorbent resin, Diaion HP-20 in broth eliminated toxic effects on growth, reduced feedback repression of teicoplanin production, and assisted In rapid recovery of teicoplanin. The teicoplanin displayed the final yield of 80% and 95% of purity.

The FMRFamide Neuropeptide FLP-20 Acts as a Systemic Signal for Starvation Responses in Caenorhabditis elegans

  • Kang, Chanhee;Avery, Leon
    • Molecules and Cells
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    • v.44 no.7
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    • pp.529-537
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    • 2021
  • Most animals face frequent periods of starvation throughout their entire life and thus need to appropriately adjust their behavior and metabolism during starvation for their survival. Such adaptive responses are regulated by a complex set of systemic signals, including hormones and neuropeptides. While much progress has been made in identifying pathways that regulate nutrient-excessive states, it is still incompletely understood how animals systemically signal their nutrient-deficient states. Here, we showed that the FMRFamide neuropeptide FLP-20 modulates a systemic starvation response in Caenorhabditis elegans. We found that mutation of flp-20 rescued the starvation hypersensitivity of the G protein β-subunit gpb-2 mutants by suppressing excessive autophagy. FLP-20 acted in AIB neurons, where the metabotropic glutamate receptor MGL-2 also functions to modulate a systemic starvation response. Furthermore, FLP-20 modulated starvation-induced fat degradation in a manner dependent on the receptor-type guanylate cyclase GCY-28. Collectively, our results reveal a circuit that senses and signals nutrient-deficient states to modulate a systemic starvation response in multicellular organisms.

A Study on the Aesthetic Consciousness of Black on Contemporary Fashion - Concentrating on the Late Twentieth Century - (현대 패션에서 나타난 블랙의 미의식에 관한 연구 - 20세기 후반을 중심으로 -)

  • Song, Myung-Hee;Cho, Kyu-Hwa
    • Journal of Fashion Business
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    • v.1 no.1
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    • pp.110-126
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    • 1997
  • This dissertation intended to investigate the black color as a fashion color of the late 20th century and the aesthetic consciousness of black fashion. Black usually expressed a negative meaning, and was regarded as a color for a mourning and a sacred dress in western culture. There found several periods of black trend specially after 14th century. Black was considered as a traditional color of men's fashion since Dandyism. By the effect of art d co fashion, the perception toward black changed to a new beauty in 20th century. There continuously found a lot of aspects of black fashion in the late 20th century. In the high fashion, popularity of black, could be seen at five different look and style : The era of line alphabet, pantaloons suit style, folklore style, mannish look and unconstructive design by Japanese, and in street fashion, it showed at four different style and fashion: Beat style, mods and rockers style, punk fashion and fetish fashion. The aesthetic consciousness of black fashion at the late 20th century mentioned above should be summarized as follows. : Minimalism, dandyism, nihilism, asceticism, eroticism. In 1990s, the black appeared under the retro mood, and it should be regarded specially as one fashion trend of inclination of simplicity. Black should be called the representative color of 20th century fashion with the reason of containing the most of aesthetic consciousness in modern fashion.

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