Archives of Pharmacal Research
- Volume 17 Issue 5
- /
- Pages.381-382
- /
- 1994
- /
- 0253-6269(pISSN)
- /
- 1976-3786(eISSN)
Administration Route Dependency of Distribution of Distribution pf PEGylated Recombinant Human Tumor Necrosis Factor Binding Protein (rhTNFbp-PEG20K dimer) following i.v. and s.c. Injection
- Kim, Dong-Chool (Synergen Inc. 1885 33rd Steet Boulder) ;
- Duane C. Bloedow (Synergen Inc. 1885 33rd Steet Boulder)
- Published : 1994.10.01
Abstract
Administration route dependency on the distribution of PEGylated recombinant human turor necrosis factor binding protein (rhTNFbp-PEG20K dimer) was observed following a subcutaneous (sc) and an intravenous (iv) administrationin rats. ehTNFbp-PEG20K dimer is composed of two rhTNGbp molecules (molecular weight 18, 278 daltons each) joined by polyethylene glycol 2000(PEG30K). The steady state distribution volume of rhTNFbp-PEG20K was 55 m/kg and 359 ml/kg following the i.v. and s.c. administrations, respectively. These results suggest that the distribution of ehTNFbp-PEG20K is limited within the cpillary space after i.v. administration, while rhTNFbp-PEG20K can distribute into a space (35.9% of body weight) which is between extracellylar space and total body water. A lymphatic absorption may paly a role in the distribution of rhTNFbp-PEF20K dimer following the sc administration. The present study suggests that the administration route of a lartge protein molecule should be determined depedning upon target sites.
Keywords
- Recombinant human tumor necrosis factor binding protein;
- Administration route dependency;
- Moment analysis;
- Polyethylene glycol;
- Steady state distribution volume