• The Journal of Advanced Prosthodontics
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• 제15권3호
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• pp.101-113
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• 2023

PURPOSE. This randomized controlled trial aimed to evaluate the effect of implants' two different diameters and cantilever lengths on the marginal bone loss and stability of mplants supporting maxillary prostheses. MATERIALS AND METHODS. Ninety-six implants were placed in sixteen completely edentulous maxillary ridges. Patients were randomly divided into two groups: Group A, implants were placed with a cantilever to anterior-posterior AP spread length (CL:AP) at a ratio of 1:3; Group B, implants were placed with a CL:AP at a ratio of 1:2. Patients were further divided into four sub-groups: Groups A1, A2, B1, and B2. Groups A1 and B1 received small diameter implants while Groups A2 and B2 received standard diameter implants. Bone height and stability measurements around each implant were performed at 0, 4, 8 and 24 months after definitive prostheses delivery. RESULTS. Statistical analysis of the mean implant stability and height values revealed an insignificant difference between Group A1 and Group A2 at all the different time intervals while significantly higher values in Group B1 in comparison with Group B2. Results also showed significantly higher values in Group A1 in comparison with Group B1 and an insignificant difference between Group A2 and Group B2 at all the different time intervals. CONCLUSION. It can be concluded that the use of small diameter implants placed with a CL:AP at a ratio of 1:3 provided predictable results and that the 1:2 CL:AP significantly induced more critical bone loss in the small diameter implants group, which can significantly reduce long term success and survival of implants

• Journal of Microbiology and Biotechnology
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• 제32권7호
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• pp.911-917
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• 2022

As valuable antibiotics, microbial natural products have been in use for decades in various fields. Among them are polyene compounds including nystatin, amphotericin, and nystatin-like Pseudonocardia polyenes (NPPs). Polyene macrolides are known to possess various biological effects, such as antifungal and antiviral activities. NPP A1, which is produced by Pseudonocardia autotrophica, contains a unique disaccharide moiety in the tetraene macrolide backbone. NPP B1, with a heptane structure and improved antifungal activity, was then developed via genetic manipulation of the NPP A1 biosynthetic gene cluster (BGC). Here, we generated a Streptomyces artificial chromosomal DNA library to isolate a large-sized NPP B1 BGC. The NPP B1 BGC was successfully isolated from P. autotrophica chromosome through the construction and screening of a bacterial artificial chromosome (BAC) library, even though the isolated 140-kb BAC clone (named pNPPB1s) lacked approximately 8 kb of the right-end portion of the NPP B1 BGC. The additional introduction of the pNPPB1s as well as co-expression of the 32-kb portion including the missing 8 kb led to a 7.3-fold increase in the production level of NPP B1 in P. autotrophica. The qRT-PCR confirmed that the transcription level of NPP B1 BGC was significantly increased in the P. autotrophica strain containing two copies of the NPP B1 BGCs. Interestingly, the NPP B1 exhibited a previously unidentified SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) inhibition activity in vitro. These results suggest that the Streptomyces BAC cloning of a large-sized, natural product BGC is a valuable approach for titer improvement and biological activity screening of natural products in actinomycetes.

• Journal of the Korean Data and Information Science Society
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• 제27권1호
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• pp.121-130
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• 2016

N명의 게임자들이 둥글게 둘러앉아 공간의존 파론도 게임 B를 실시한다. 게임 B는 여러 명의 게임자들 중에서 한 명을 임의로 선택하고, 선택된 게임자는 양 옆에 있는 두 명의 게임자들의 상태에 따라 앞면이 나올 확률이 달라지는 동전을 던져서 앞면이 나오면 1원을 얻고 뒷면이 나오면 1원을 잃는다. 게임 A'은 임의로 선택된 게임자가 나머지 N - 1명의 게임자들 중에서 한 명을 임의로 선택하여 본인의 상금 1원을 전달하는 게임으로 전체 게임자들의 총 상금에는 변함이 없으므로 전체 게임자들에게는 항상 공정한 게임이다. 만약 게임 B가 지는 게임인 반면에 두 게임 A'와 B를 결합한 혼합게임 C는 이기는 게임이 되면 파론도 효과가 존재하고, 게임 B가 이기는 게임이고 혼합게임 C는 지는 게임이면 역파론도 효과가 존재한다고 한다. 먼저 마코프 체인의 상태공간의 축소를 위한 lumpability 조건이 게임 A', B 그리고 혼합게임 C에 대해 만족함을 보이고, 축소된 상태공간에서 게임 B와 C의 기대상금을 계산한다. 이를 이용하여 파론도 효과와 역파론도 효과의 존재를 확인하고, 특별히 $3{\leq}N{\leq}6$의 경우에는 파론도 효과와 역파론도 효과가 존재하는 확률 모수의 영역을 도식화 한다.

• 말소리와 음성과학
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• 제7권1호
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• pp.21-26
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• 2015

The aim of this study is to synthesize pathological breathy voice and to make a cepstral peak prominence (CPP) table following breathiness ranks by cepstral analysis to supplement reliability of the perceptual auditory judgment task. KlattGrid synthesizer included in Praat was used. Synthesis parameters consist of two groups, i.e., constants and variables. Constant parameters are pitch, amplitude, flutter, open phase, oral formant and bandwidth. Variable parameters are breathiness (BR), aspiration amplitude (AH), and spectral tilt (TL). Five hundred sixty samples of synthetic breathy vowel /a/ for male were created. Three raters participated in ranking of the breathiness. 217 were proved to be inadequate samples from perceptual judgment and cepstral analysis. Finally, 343 samples were selected. These CPP values and other related parameters from cepstral analysis are classified under four breathiness ranks (B0~B3). The mean and standard deviation of CPP is $16.10{\pm}1.15$ dB(B0), $13.68{\pm}1.34$ dB(B1), $10.97{\pm}1.41$ dB(B2), and $3.03{\pm}4.07$ dB(B3). The value of CPP decreases toward the severe group of breathiness because there is a lot of noise and a small quantity of harmonics.

• 대한기계학회논문집B
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• 제23권3호
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• pp.311-319
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• 1999

The thermal conductivity ${\lambda}_b$, thermal diffusivity ${\alpha}_b$ of the thin black paint layer sprayed on solid surface and absorptivity a for laser beam are measured by applying a non-contact measurement method of the thermophysical properties of solids. The values of a=0.67, ${\lambda}_b=1.45W/mK$ and ${\alpha}_b=1.24{\times}10^{-6}m^2/s$ are obtained for the sprayed lay~ thickness $z_b{\fallingdotseq}40\;{\mu}m$. Furthermore, for the $z_b{\fallingdotseq}24\;{\mu}m$ thick layers which arc formed by rubbing with a glass rod after spraying, the values of a=0.73, ${\lambda}_b=1.85W/mK$. and ${\alpha}_b=1.09{\times}10^{-6}m^2/s$ are obtained. It is also shown that the present thermal diffusivity ${\alpha}_b$ for $z_b{\fallingdotseq}40\;{\mu}m$ Is about 30~80% larger than those obtained by Araki et al. for the thicker layer $z_b{\fallingdotseq}150{\sim}248\;{\mu}m$. This method could be applied to the measurement of thermophysical properties of thin layer on solids.

• 한국자기학회지
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• 제5권1호
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• pp.38-41
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• 1995

$\alpha$-Fe를 주상으로 하는 새로운 Nd-Fe-B계 합금을 개발하기 위하여 Nd 함유량을 4at.%로 고정시킨 Nd-Fe-B 초미세결정립합금의 제조 및 자기특성이 조사되었다. 급속응고법으로 제조된 $Nd_{4}Fe_{85.5}B_{10.5}$ 비정질합금은 결정화하여 $\alpha$-Fe 기지상에 $Nd_{2}Fe_{14}B$이 형성되나 자기특성${_{i}H_{c}=95.5kA/m(1.2kOe),\;Br=1.2T}$은 열화된다. Nb 및 Cu를 첨가한 $Nd_{4}Fe_{82}B_{10}Nb_{3}Cu_{1}$ 합금은 $\alpha$-Fe 결정립미세화(<30nm)로 보자력이 207kA/m(2.6 kOe)로 증가하나 잔류자화는 개선되지 않았다. 이 합금조성에 8at.% Co 첨가는 결정립을 더욱 미세화시키며 자기특성을 개선 시킨다. 최적열처리조건에서 $Nd_{4}Fe_{74}Co_{8}B_{10}Nb_{3}Cu_{1}$ 합금의 잔류자화, 보자력 및 최대에너지적이 각각 1.34 T, 219 kA/m (2.75kOe) 및 $95.5kJ/m^{3}$(12MGOe) 이다.

• 한국동물생명공학회지
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• 제37권2호
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• pp.121-129
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• 2022

Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) is an N6-methyladenosine (m⁶A) RNA modification regulator and a key determinant of prem-RNA processing, mRNA metabolism and transportation in cells. Currently, m⁶A reader proteins such as hnRNPA2/B1 and YTHDF2 has functional roles in mice embryo. However, the role of hnRNPA2/B1 in porcine embryogenic development are unclear. Here, we investigated the developmental competence and mRNA expression levels in porcine parthenogenetic embryos after hnRNPA2/B1 knock-down. HhnRNPA2/B1 was localized in the nucleus during subsequent embryonic development since zygote stage. After hnRNPA2/B1 knock-down using double stranded RNA injection, blastocyst formation rate decreased than that in the control group. Moreover, hnRNPA2/B1 knock-down embryos show developmental delay after compaction. In blastocyste stage, total cell number was decreased. Interestingly, gene expression patterns revealed that transcription of Pou5f1, Sox2, TRFP2C, Cdx2 and PARD6B decreased without changing the junction protein, ZO1, OCLN, and CDH1. Thus, hnRNPA2/B1 is necessary for porcine early embryo development by regulating gene expression through epigenetic RNA modification.

• 한국안광학회지
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• 제8권1호
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• pp.53-57
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• 2003

5분 간격으로 착색시간을 증가시켜 R, G, Y, B칼라렌즈를 제작하였다. 색 좌표 분석은 분광측색계와 CIE $L^*a^*b^*$ color system를 사용하였다. 착색 시간에 따른 색좌표 이동 형태는 $b^*(Y-B)={\beta}a^*+{\alpha}$이며, 거의 선형적으로 변하였다. 적색의 경우 착색시간이 길수록 $G{\rightarrow}R$, $B{\rightarrow}Y$ 형태로 이동하고, Parameter ${\alpha}$, ${\beta}$ 값은 각각 -3.49, 0.90을 얻었다. 녹색의 경우 색좌표 이동은 $R{\rightarrow}G$, $B{\rightarrow}Y$ 형태이다. 10분의 착색 시간에서 가장 순수한 녹색 칼라를 얻었다. Parameter ${\alpha}$, ${\beta}$값은 각각 -0.72, -0.55을 얻었다. 황색과 청색의 경우 모두 착색 시간이 길수록 순수한 색이 된다. 황색에서 Parameter ${\alpha}$, ${\beta}$값은 각각 14.11, 1.58이고, 청색에서 Parameter ${\alpha}$, ${\beta}$값은 각각 -11.62, 1.30을 얻었다.

• Bulletin of the Korean Chemical Society
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• 제17권10호
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• pp.909-918
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• 1996

Syntheses of carbapenam skeletons were achieved from 1,3-propanediol through 1,3-dipolar cycloaddition. 3-(Tetrahydropyran-2-yloxy)-(10) and 3-(t-butyldimethylsilyloxy) propanal (13) were obtained from 1,3-propanediol. 3-Hydroxypropanals (10, 13, 14) were reacted with N-hydroxyglycine esters to give C-(2-hydroxyethyl)-N-alkoxycarbonylmethylnitrones (15a-15d). 1,3-Dipolar cycloaddition of the nitrones with methyl acrylate or ethyl crotonate gave 3-(2-hydroxyethyl)isoxazolidines (16a-16b, 17a-17b, 18, 19a-19b). 3-(2-Hydroxyethyl)isoxazolidines (17a, 17c, 19a, 19b) were converted to 3-(2-iodoethyl)isoxazolidines (21a-21d) or 3-phenylthiocarbonylmethylisoxazolidines (25a-25d) which were cyclized to give 2-oxa-1-azabicyclo[3.3.0]octanedicarboxylates (22a-22d, 26a-26d). 2-Oxa-1-azabicyclo[3.3.0]octane-4,8-dicarboxylates (22c-22d, 26c-26d) were transformed to 6-(l-hydroxyethyl)carbapenam-3-carboxylates (30a-30b, 31a-31b).

• 약학회지
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• 제39권2호
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• pp.205-209
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• 1995

Methylene protons of benzyl and ethyl ester in ethyl 1-benzyl-2, 3-dioxo-cyclopenta[b]-pyrrole-3a-carboxylate (A) exhibited opposite$^{1}$H-NMR spectral patterns mutually between magnetic equivalence and nonequivalence depending on concentration and temperature. The diastereotopic spectral data of compound A were reported with brief interpretation.