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Metabolism of Lactate Dehydrogenase in Tissues from Ldh-C Expressed Fish at Starved State (기아상태에서 Ldh-C가 발현된 어류 조직의 젖산탈수소효소의 대사)

  • Yum, Jung Joo;Kim, Gyu Dong
    • Journal of Life Science
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    • v.26 no.2
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    • pp.155-163
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    • 2016
  • Metabolism of lactate dehydrogenase (EC 1.1.1.27, LDH) was studied to identify the function of LDH-C. Tissues of LDH liver-specific Ldh-C expressed Carassius auratus and eye-specific Ldh-C expressed Lepomis macrochirus after starvation were studied. LDH activity in liver tissue from C. auratus was increased after starvation. And LDH specific activity (units/mg) and LDH/CS were increased in tissues. It means the anaerobic metabolism was taking place in C. auratus after starvation. LDH B4 isozyme was decreased in skeletal muscle and increased in heart tissue. LDH C4 isozymes those showed in eye and brain tissues were identified as liver-specific C4 isozymes and disappeared after starvation. And C hybrid in eye, A4 isozyme in brain, and both C hybrid and C4 isozyme in liver tissue were increased, respectively. In L. macrochirus, the level of variation of LDH activities was low but greatly increased especially in eye tissue and LDH A4 and AC hybrid were increased in brain tissue. The LDH activities in tissues from C. auratus and L. macrochirus remained 30.30-18.64% and 25-18.75%, respectively, as a result of the inhibition by 10 mM of pyruvate. The KmPYR values of LDH in C. auratus were increased. As a result, LDH liver-specific C4 isozyme was expressed in liver, brain and eye tissues during starvation. It seems metabolism of lactate was predominant in brain tissue. After starvation, the liver-specific LDH-C was affected more than eye-specific LDH-C.

The characteristics of polycrystalline 3C-SiC microhotplates for high temperature M/NEMS (고온 M/NEMS용 3C-SiC 마이크로 히터 특성)

  • Jeong, Jae-Min;Chung, Gwiy-Sang
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.11a
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    • pp.252-252
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    • 2008
  • The microhotplates consisting of a Pt-ased heating element on AlN/poly 3C-SiC layers were fabricated. The microhotplate has a $600{\mu}m{\times}600{\mu}m$ square shaped membrane which made of $1{\mu}m$ thick ploycrystalline 3C-SiC suspended by four legs. 3C-SiC is known for excellent chemical durability, mechanical strength and sustaining of high temperature. The membrane is fabricated by surface micromachining using oxidized Si sacrificial layer. The Pt thin film is used for heating material and resist temperature sensor. The fabrication methodology allows intergration of an array of heating material and resist temperature detector. For reasons of a short response time and a high sensitivity a uniform temperature profile is desired. The dissipation of microhotplate was examined by a IR thermoviewer and the power consumption was measured. Measured and simulated results are compared and analyzed. Thermal characterization of the microhotplates shows that significant reduction in power consumption was achieved using suspended structure.

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A Study on the Prediction of the Permanent Wilting Point in Woody Plant by Cambial Electrical Resistance (목본식물의 형성층 전기저항에 의한 영구위조점 예측에 관한 연구)

  • 김민수
    • Journal of the Korean Institute of Landscape Architecture
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    • v.22 no.4
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    • pp.75-80
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    • 1995
  • It is important to estimate the possibility of recovery in physiologically damaged woody plant. It is suggested that C.E.R(cambial electrical resistance) might be a useful method to predict the permanent wilting point. D/A and A/D converter can be used to measure the C.E.R and it took only 10-20 msec for a measurement and the values were stable during this study. A computer could be used for the continual measurement of C.E.R. There were very big daily changes of C.E.R. was changed according to the changes of indoor temperature, but the phase was slightly different. It is reasoned that daily changes in C.E.R. is induced by the changes of water potential and cambial thickness. It was difficult to detect the changes of C.E.R. caused by changes in soil moisture under high soil water potential. Under low soil water potential, the changes in soil moisture under high soil water potential. Under low soil water potential, the changes of C.E.R. can be detected. After wilting, C.E.R. is increased very rapidly. When C.E.R. is not decreased by watering, it will be permanent wilting point. But it takes several days to confirm the permanent wilting point. To predict the possibility of recovery from wilting, the values of C.E.R. have no meaning. But the changes of C.E.R. are significant. Therefore we can predict the permant wilting point in woody plant by monitoring the change of C.E.R. by the computer.

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A study on Properties of YbBaCuO Superconductor with various calcination conditions (하소 조건 변화에 따른 YbBaCuO 초전도체 의 특성 연구)

  • 이영매;박정철;소대화
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1997.11a
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    • pp.68-72
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    • 1997
  • In this paper, to obtain the YbB $a_2$C $u_3$ $O_{x}$ superconductor, the mixed Powders of Y $b_2$ $O_3$, BaC $O_3$, CuO and Y $b_2$BaCu $O_{5}$, BaCu $O_2$ were used and the various calcining conditions were applied for the 123 phase of YbB $a_2$C $u_3$ $O_{x}$. Samples were prepared by the mixed oxide method and calcined with various temperatures of 88$0^{\circ}C$ ~91$0^{\circ}C$ . It was observed that the distribution of YbB $a_2$C $u_3$ $O_{x}$ phase which was calcined at 90$0^{\circ}C$ for 12 hours and 99 hours. But the result of long time calcination(99 hrs), the 123 phase of YbB $a_2$C $u_3$ $O_{x}$ was existed between 89$0^{\circ}C$ and 91$0^{\circ}C$ . And the best case could be obtained at the calcination temp. of 90$0^{\circ}C$ from the mixed Powder of YbB $a_2$C $u_3$ $O_{5}$ and Bacu $O_2$ which were prepared individually.idually.

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ON A NEW CLASS OF DOUBLE INTEGRALS INVOLVING GENERALIZED HYPERGEOMETRIC FUNCTION 3F2

  • Kim, Insuk
    • Honam Mathematical Journal
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    • v.40 no.4
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    • pp.809-816
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    • 2018
  • The aim of this research paper is to evaluate fifty double integrals invoving generalized hypergeometric function (25 each) in the form of $${{\int}^1_0}{{\int}^1_0}\;x^{{\gamma}-1}y^{{\gamma}+c-1}(1-x)^{c-1}(1-y)^{c+{\ell}}(1-xy)^{{\delta}-2c-{\ell}-1}{\times}_3F_2\[{^{a,\;b,\;2c+{\ell}+1}_{\frac{1}{2}(a+b+i+1),\;2c+j}}\;;{\frac{(1-x)y}{1-xy}}\]dxdy$$ and $${{\int}^1_0}{{\int}^1_0}\;x^{{\gamma}-1}y^{{\gamma}+c+{\ell}}(1-x)^{c+{\ell}}(1-y)^{c-1}(1-xy)^{{\delta}-2c-{\ell}-1}{\times}_3F_2\[{^{a,\;b,\;2c+{\ell}+1}_{\frac{1}{2}(a+b+i+1),\;2c+j}}\;;{\frac{1-y}{1-xy}}\]dxdy$$ in the most general form for any ${\ell}{\in}{\mathbb{Z}}$ and i, j = 0, ${\pm}1$, ${\pm}2$. The results are derived with the help of generalization of Edwards's well known double integral due to Kim, et al. and generalized classical Watson's summation theorem obtained earlier by Lavoie, et al. More than one hundred ineteresting special cases have also been obtained.

Updated Meta-analysis of the Association Between CYP2E1 RsaI/PstI Polymorphisms and Lung Cancer Risk in Chinese Population

  • Wang, Ya-Dong;Yang, Hai-Yan;Liu, Jing;Wang, Hai-Yu
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.13
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    • pp.5411-5416
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    • 2014
  • Background: A number of studies have reported relationships of CYP2E1 RsaI/PstI polymorphisms with susceptibility to lung cancer in Chinese population. However, the epidemiologic results have been conflictive rather than conclusive. The purpose of this study was to address the associations of CYP2E1 RsaI/PstI polymorphisms with lung cancer risk in Chinese population comprehensively. Materials and Methods: Systematic searches were conducted in the PubMed, Science Direct, Elsevier, CNKI and Chinese Biomedical Literature Databases. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to estimate the strength of association. Results: Overall, we observed a decreased lung cancer risk among subjects carrying CYP2E1 RsaI/PstI c1/c2 and c1/c2+c2/c2 genotypes (OR=0.76, 95%CI: 0.64-0.90 and OR=0.78, 95%CI: 0.66-0.93, respectively), as compared with subjects carrying the c1/c1 genotype. In subgroup analysis, we observed a decreased lung cancer risk among c1/c2 carriers in hospital-based studies (OR=0.81, 95%CI: 0.68-0.98) and among carriers with c1/c2 and c1/c2+c2/c2 genotypes in population-based studies(OR=0.57, 95%CI: 0.42-0.79 and OR=0.58, 95%CI: 0.43-0.79, respectively), as compared with subjects carrying the c1/c1 genotype. Limiting the analysis to studies with controls in Hardy-Weinberg equilibrium (HWE), we similarly observed a decreased lung cancer risk among c1/c2 and c1/c2+c2/c2 carriers (OR=0.73, 95%CI: 0.60-0.88 and OR=0.73, 95%CI: 0.60-0.88, respectively), as compared with c1/c1. Conclusions: Our results suggested that CYP2E1 RsaI/PstI c1/c2 and c1/c2+c2/c2 variants might be a protective factor for developing lung cancer in Chinese population. Further well-designed studies with larger sample size are required to verify our findings.

Nectandrin A Enhances the BMP-Induced Osteoblastic Differentiation and Mineralization by Activation of p38 MAPK-Smad Signaling Pathway

  • Kim, Do Yeon;Kim, Go Woon;Chung, Sung Hyun
    • The Korean Journal of Physiology and Pharmacology
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    • v.17 no.5
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    • pp.447-453
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    • 2013
  • Osteoblastic activity of nectandrin A was examined in C2C12 cells. Nectandrin A enhances the BMP-induced osteoblastic differentiation and mineralization, manifested by the up-regulation of differentiation markers (alkaline phosphatase and osteogenic genes) and increased calcium contents. In C2C12 cells co-transfected with expression vector encoding Smad4 and Id1-Luc reporter, nectandrin A increased Id1 luciferase activity in a concentration-dependent manner, when compared to that in BMP-2 treated cells, indicating that Smad signaling pathway is associated with nectandrin A-enhanced osteoblastic differentiation in C2C12 cells. In addition, nectandrin A activated p38 mitogen-activated protein kinase (MAPK) in time- and concentration-dependent manners, and phosphorylated form of pSmad1/5/8 and alkaline phosphatase activity were both decreased when the cells were pretreated with SB203580, a p38 MAPK inhibitor, suggesting that p38 MAPK might be an upstream kinase for Smad signaling pathway. Taken together, nectandrin A enhances the BMP-induced osteoblastic differentiation and mineralization of C2C12 cells via activation of p38 MAPK-Smad signaling pathway, and it has a therapeutic potential for osteoporosis by promoting bone formation.

External Condensation Heat Transfer Coefficients of R22 Alternative Refrigerants and R134a According to the Saturated Vapor Temperature Change on a Smooth Tube (수평관에서 R22 대체냉매 및 R134a의 포화증기 온도변화에 따른 외부 응축 열전달계수에 관한 연구)

  • Yoo Gil-Sang;Hwang Ji-Hwan;Park Ki-Jung;Jung Dongsoo
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.17 no.8
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    • pp.729-735
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    • 2005
  • In this study, external condensation heat transfer coefficients (HTCs) were measured on a horizontal smooth tube at the saturated vapor temperature of $30^{\circ}C,\;39{\circ}C,\;and\;50^{\circ}C$ for R22, R410A, R407C, and R134a with the wall subcooling of $3\~8^{\circ}C$. The HTCs of all refrigerants are the highest at $30^{\circ}C,\;39{\circ}C,\;and\;50^{\circ}C$ in order. This trend is due to its excellent thermodynamic properties of the liquid phase. The measured data of HTCs were compared with the calculated ones by Nusselt's equation for a smooth tube. Measured HTCs of R22, R134a, R410A are $4.2\~7.5\%$ higher than prediction respectively while those of R407C are $15.6\~28.9\%$ lower than the prediction.

HOMOGENEOUS $C^*$-ALGEBRAS OVER A SPHERE

  • Park, Chun-Gil
    • Journal of the Korean Mathematical Society
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    • v.34 no.4
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    • pp.859-869
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    • 1997
  • It is shown that for $A_{k, m}$ a k-homogeneous $C^*$-algebra over $S^{2n - 1} \times S^1$ such that no non-trivial matrix algebra can be factored out of $A_{k, m}$ and $A_{k, m} \otimes M_l(C)$ has a non-trivial bundle structure for any positive integer l, we construct an $A_{k, m^-} C(S^{2n - 1} \times S^1) \otimes M_k(C)$-equivalence bimodule to show that every k-homogeneous $C^*$-algebra over $S^{2n - 1} \times S^1)$. Moreover, we prove that the tensor product of the k-homogeneous $C^*$-algebra $A_{k, m}$ with a UHF-algebra of type $p^\infty$ has the tribial bundle structure if and only if the set of prime factors of k is a subset of the set of prime factors of pp.

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repABC- Type Replicator Region of Megaplasmid pAtC58 in Agrobacterium tumefaciens C58

  • LEE KO-EUN;PARK DAE-KYUN;BAEK CHANG-HO;HWANG WON;KIM KUN-SOO
    • Journal of Microbiology and Biotechnology
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    • v.16 no.1
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    • pp.118-125
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    • 2006
  • The region responsible for replication of the megaplasmid pAtC58 in the nopaline-type Agrobacterium tumefaciens strain C58 was determined. A derivative ofa Co1E1 vector, pBluscript SK-, incapable of autonomous replication in Agrobacterium spp, was cloned with a 7.6-kb Bg1II-HindIII fragment from a cosmid clone of pAtC58, which contains a region adjacent to the operon for the utilization of deoxyfructosyl glutamine (DFG). The resulting plasmid conferred resistance to carbenicillin on the A. tumefaciens strain UIA5 that is a plasmidfree derivative of C58. The plasmid was stably maintained in the strain even after consecutive cultures for generations. Analysis of nested deletions of the 7.6-kb fragment showed that a 4.3-kb BglII-XhoI region sufficiently confers replication of the derivative of the ColE1 vector on UIA5. The region comprises three ORFs, which have high homologies with repA, repB, and repC of plasm ids in virulent Agrobacterium spp. including pTiC58, pTiB6S3, pTi-SAKURA, and pRiA4b as well as those of symbiotic plasmids from Rhizobium spp. Phylogenie analysis showed that rep genes in pAtC58 are more closely related to those in pRiA4 than to pTi plasmids including pTiC58, suggesting that the two inborn plasmids, pTiC58 and pAtC58, harbored in C58 evolved from distinct origins.