• Korean Journal of Plant Taxonomy
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• v.44 no.4
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• pp.247-249
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• 2014

A newly naturalized plant, Carex scoparia Schkuhr ex Willd. var. scoparia, was found in Mt. Hanseok, Injegun, Gangwon-do, Korea. It belongs to sect. Ovales Kunth and had been known from North America, and was introduced into Europe and New Zealand. This species distinguished from related taxa in Asia, Carex maackii Maxim. and Carex leporina L., by pistillate scales lanceolate and having large perigynia. We propose the new Korean name, 'Han-seok-sa-cho', based on its first collecting place. We also provide descriptions for morphological characters, illustrations, photographs and keys to allied taxa.

• Korean Journal of Plant Taxonomy
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• v.41 no.1
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• pp.1-9
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• 2011

Artemisia capillaris Thunb. and A. scoparia Waldst. & Kit. are classified by their root shape, leaf size and capitulum size. However, these characteristics are not nearly as useful when attempting to identify the two species owing to the continuous variation in them. Another important taxonomic characteristics is the habit, whether it is the subshrubs or perennial herbs of A. capillaris Thunb. or the annual or biennial herbs of A. scoparia Waldst. & Kit.. All observed specimens and living individuals in Korea are subshrubs or perennial herbs, and annual or biennial examples are not found. Therefore, it is considered that A. scoparia Waldst. & Kit. is not distributed in Korea.

• The Korean Journal of Ecology
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• v.19 no.4
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• pp.295-304
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• 1996

KDICical substances from Artemisia scoparia were analyzed by gas chromatography. Seven phenolic compounds and thirty nine terpenoids were identified. Most abundant within each group were cinnamic acid and camphor, respectively. The KDICicals were prepared as aqueous extracts and then used for germination, growth, and chlorophyll content tests. The extracts were inhibitory to germination and seedling growth of the receptor lants. This inhibitory effect was dependent on concentration. When the effect of the aqueous extract on chlorophyll content was assayed, both chlorophylls a and b were shown to be reduced. The reduction in seedling elongation and growth in dry weight paralleled the reduction in chlorophyll concentration. These KDICical substances, including phenolic compounds and terpenoids, from Artemisia scoparia were responsible for the growth inhibition of the selected species.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.323-330
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• 2019

The high expression level of industrial and metabolically important proteins in plants can be achieved by plastid transformation. The CaIA vector, a Capsicum-specific vector harboring aadA (spectinomycin resistance), is a selectable marker controlled by the PsbA promoter, and the terminator is flanked by the trnA and trnI regions of the inverted repeat (IR) region of the plastid. The CaIA vector can introduce foreign genes into the IR region of the plastid genome. The biolistic method was used for chloroplast transformation in Scoparia dulcis with leaf explants followed by antibiotic selection on regeneration medium. Transplastomes were successfully screened, and the transformation efficiency of 3 transgenic lines from 25 bombarded leaf explants was determined. Transplastomic lines were evaluated by PCR and Southern blotting for the confirmation of aadA insertion and its integration into the chloroplast genome. Seeds collected from transplastomes were analyzed on spectinomycin medium with wild types to determine genetic stability. The increased chloroplast transformation efficiency (3 transplastomic lines from 25 bombarded explants) would be useful for expressing therapeutically and industrially important genes in Scoparia dulcis L.

• The Korean Journal of Oral and Maxillofacial Pathology
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• v.42 no.6
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• pp.167-174
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• 2018

Natural products are vastly utilized as a source of chemotherapeutic agents for human cancers. Kochia scopraia is traditionally used for the cure of urological and dermatological diseases. Recently, methanol extract of Kochia scoparia (MEKS) has been shown to have anti-cancer activity to various human cancers. However, there is no report demonstrating the anti-cancer activity of MEKS in human mucoepidermoid carcinoma (MEC) cells. In this study, the authors studied the effects of MEKS on the cell proliferation and underlying mechanism in YD15 human MEC cells. MEKS decreased YD15 cell proliferation proven by trypan blue exclusion assay and induced apoptosis, evidenced by cell cycle analysis and western blotting. Autophagy induction by MEKS was verified by western blotting. In addition, MEKS regulated the expression of phosphorylated Akt, phosphorylated p38 and Nrf2 protein. This results can imply that MEKS might be a potential candidate for the treatment of human MEC cells.

• Korean Journal of Pharmacognosy
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• v.37 no.4 s.147
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• pp.235-240
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• 2006

We investigated the antioxidant activities such as DPPH radical scavenging capacity, xanthine oxidase inhibitory activity, and superoxide radical scavenging capacity of the aqueous EtOH extract and its solvent fractions of Artemisia scoparia. The ethyl acetate fraction showed high antioxidant activity, compared to positive controls such as ascorbic acid, butylated hydroxy anisole (BHA), trolox, and allopurinol in these assay systems. Moreover, we examined the inhibitory effect of solvent fractions of A. scoparia on the production of pro-inflammatory factors that the nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and prostaglandin E2 $(PGE_2)$ production activated with LPS $(1{\mu}g/ml)$ in murine macrophage cell line RAW264.7. The amounts of protein levels were determined by immunoblottting. Tn the sequential fractions of hexane and dichloromethane inhibited the NO and $PGE_2$ production and the protein level of iNOS and COX-2. These results suggest that A. scoparia may have anti-inflammatory activity through the antioxidant activity and inhibition of pro-inflammatory factors.

• Journal of Life Science
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• v.28 no.9
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• pp.999-1006
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• 2018

Obesity is epidemic worldwide and has reportedly been linked to the progression of several metabolic and cardiovascular diseases. The natural products are decreasing the side effects of medicines used for obesity and also have health benefits dut to their numerous bioactive compounds. In this context, Artemisia scoparia is a widespread plant that has been suggested as possessing various types of bioactivity. In this study, the crude extract from A. scoparia (ASE) was tested for its ability to suppress adipogenesis in mouse 3T3-L1 pre-adipocytes. The molecular pathway by which ASE affects differentiation of 3T3-L1 cells was also investigated. The introduction of ASE to differentiating 3T3-L1 pre-adipocytes resulted in suppressed adipogenesis, as confirmed by decreased intracellular lipid accumulation. The differentiating cells treated with 10 and $100{\mu}g/ml$ of ASE showed 21.9 and 29.0% less lipid accumulation, respectively, than untreated adipocytes. In addition, the results indicated that ASE treatment lowered the expression of the adipogenesis-related factors $PPAR{\gamma}$, $C/EBP{\alpha}$, and SREBP-1c. Furthermore, treating with ASE notably decreased levels of phosphorylated p38, ERK, and JNK in 3T3-L1 adipocytes. These results indicate that ASE exhibits significant anti-adipogenesis activity by downregulating the MAPK and $PPAR{\gamma}$ pathways during the differentiation of 3T3-L1 pre-adipocytes. Therefore, A. scoparia may be a potential source of natural products against obesity.

• Korean Journal of Environment and Ecology
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• v.20 no.3
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• pp.311-318
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• 2006

In an estuary, ecological dynamic is modified for a long time and memorized in soils and landscapes. As landscape memory, ecological motif is defined with dominance and rarity of vegetation. The purpose of the study is to investigate the existing vegetation character and to propose the ecological motif of this area. The present salt-water plants, community species composition and constancy degree around the estuary in Buandam watershed Buan-gun, Jeollabuk-do were analyzed. The results are as follows. The flora of the salt-water plants was listed as 16 taxa; 6 families,13 genera,14 species and 2 varieties. 5 taxa were Gramineae, 4 taxa Chenopodiaceae, and 4 taxa Comrositae. The salt-water plant communities are a total of 10 communities as listed; Zoysia sinica-Artemisia scoparia community, Phacelurus latifolius community, Artemisia scoparia Cnidium japonicum community, Limonium tetragonum -Artemisia scoparia community, Artemisia scoparia community, Suaeda japonica community, Elymus dahuricus community, Suaeda asparagoides community, Zoysia sinica community, and Zoysia sinica-Suaeda japonica community. Analyzed by the community classification species class of constancy degree, Phacelurus latifolius, Cnidium japonicum, Limonium tetragonum, Suaeda japonica, Elymus dahuricus and Suaeda asparagoides belong to I, Zoysia sinica and Artemisia scoparia to II. Elymus dahuricus, Setaria viridis var. pachystachys, Echinochloa crusgalli var. oryzicola, Phacelurus latifolius, Atriplex gmelini, Salicornia herbacea, Calystegia soldanella and Aster tripolium belong to the accompaniment species to I: Zoysia sinica, Suaeda asparagoides, Artemisia capillaris to II; Suaeda japonica, Artemisia scoparia to III ; Cnidium japonicum to IV: Limonium tetragonum, Artemisia fukudo to V. And the ecological motif is the Phacelurus latifolius community.

• Archives of Pharmacal Research
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• v.25 no.3
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• pp.336-342
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• 2002

MeOH extract of Kochia scoparia was fractionated into $CHCl_3-$, EtOAc- and BuOH extracts and the last fraction were hydrolyzed by 3%-NaOH ($MeOH-H_2O$) to compare the bioactivities on antinociceptive and anti-inflammatory effects. Silica gel column chromatography of BuOH fraction afforded a large amount of $3-Ο-{\beta}-D-xylopyranosyl {\;}(1{\rightarrow}3)-{\beta}-D-glucuronopyranosyl$ oleanolic acid (momordin Ic, 4) and that of acid hydrolysate of BuOH fraction gave $3-Ο-{\beta}-D-glucuronopyranosyl oleanolic$ acid (momordin Ib, 3), its 6'-Ο-methyl ester (2) and oleanolic acid (1). Silica gel column chromatography of alkaline hydrolysate afforded a large amount of 4. MeOH extract and both EtOAc- and BuOH fractions were active in the rheumatoidal rat induced Freund's complete adjuvant reagent (FCA) whereas $CHCl_3$ fraction was inactive. Compound 1 and 4 showed significant activities in the same assay but oleanolic acid 3-Ο-glucuronopyranoside (3) showed no activity. These fashions were also observed in carrageenan-induced edema of the rat and in the antinociceptive activity tests undertaken in hot plate- and writhing methods. These results suggest that momordin Ic and its aglycone, oleanolic acid, could be active principles for rheumatoid arthritis.

• Journal of Life Science
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• v.31 no.12
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• pp.1100-1109
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• 2021

The halophyte Artemisia scoparia is an edible medicinal plant, with insecticidal, anti-inflammatory, anticholesterol, antipyretic, and antibacterial effects. The aim of this study was to assess the inhibitory effect of crude extract and solvent-partitioned fractions obtained from A. scoparia on MMP-2 and MMP-9 activity in phorbol-12-myristate-13-acetate (PMA)-stimulated human fibrosarcoma HT-1080 cells using four different activity tests: gelatin zymography, MMP enzyme-linked immunosorbent assay (ELISA), wound healing assay, reverse transcription-polymerase chain reaction (RT-PCR), and Western blot assay. A. scoparia samples were extracted twice with methylene chloride (MC) and twice with methanol (MeOH). After the MC and MeOH crude extracts were combined, the combined crude extracts showed a significant inhibitory effect against MMP-2 and MMP-9 enzymes. They were then fractionated into n-hexane, 85% (v/v) aqueous methanol (85% (v/v) aq.MeOH), n-butanol, and water according to solvent polarity. Among the four solvent-partitioned fractions, n-hexane and 85% (v/v) aq. MeOH fractions significantly inhibited MMP-2 and MMP-9 activity and cell mobility. In addition, the n-hexane and 85% (v/v) aq.MeOH fractions effectively inhibited MMP-2 and -9 activity in the gelatin zymography and MMP ELISA assay. In the wound healing assay, RT-PCR, and Western blot assay, all solvent-partitioned fractions, except the H₂O fraction, significantly suppressed cell migration, as well as the expression levels of MMP-2 and -9 mRNA and proteins.