• Plant Resources
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• v.3 no.3
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• pp.179-183
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• 2000

The present study was carried out to clarify the chromosome numbers and karyotype of Atractylodes japonica Koidz. ex Kitam. and A. macrocephala Koidz.. The somatic chromosome numbers of two species were same; basic chromosome number x=12, and somatic chromosome numbers 2n=24. The present result of A. japonica Koidz. ex Kitam. was same to previously reports and that of A. macrocephala Koidz. was reported first in this study. Size and shape of chromosome were some different from A. japonica Koidz. ex Kitam. and A. macrocephala Koidz.. The karyotype of A. japonica Koidz. ex Kitam. was described as follows; 2n : 24 : 8L + 14M +2S : 2 $A^{sm}$ +2 $B^{m}$ +2 $C^{m}$ +2 $D^{st}$ + 2 $E^{m}$ +2 $F^{m}$ +2 $G^{m}$ +2 $H^{sm}$ + 2 $I^{m}$ + 2 $J^{m}$ + 2 $K^{m}$ + 2 $L^{m}$ . And the karyotype of A. macrocephata Koidz. was described as follows; 2n : 24 : 10L +12M +25 : 2 $A^{m}$ +2 $B^{sm}$ +2 $C^{sm}$ +2 $D^{sm}$ +2 $E^{sm}$ +2 $E^{sm}$ +2 $G^{sm}$ +2 $H^{m}$ +2 $I^{m}$ 2 $J^{m}$ +2 $K^{m}$ +2 $L^{m}$ . .

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.2
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• pp.348-352
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• 2002

This study was carried out to evaluate cytotoxic effects of Atractylodes macrocephala Koidz. (A. macrocephala Koidz.) extract on NIH 3T3 fibloblast. SK-MEL-3 (HBT 69) and KB (ATCC No, OCL 17) cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. 10.2 mg/ml Concentration of A. macrocephala Koidz. extracts in SK-MEL-3 showed that their susceptibility (sensitivity) to these compounds decreased in the following order ; adriamycin > H₂O > ethyl acetate > ethyl alcohol > chloroform > n-hexane in SK-MEL-3 cell lines ; 5-FU > H₂O > n-hexane > ethyl acetate > ethyl alcohol > chloroform in KB cell lines. In order to develop an antimicrobial agent, A. macrocephala Koidz. was extracted with solvents. The minimal inhibitory concentrations (MICs) of each solvent extract of A. macrocephala Koidz. against microogranisms were also examined. Antimicrobial activities of ampicillin and ketoconazole as references were compared to those of each solvent extract of A. macrocephala Koidz. The antimicrobial activity of the ethyl acetate soluble extract of A. macrocephala Koidz. had growth inhibition activity against S. mutans and P. putida (MICs. 500 ㎍/ml). These results suggest that the ethyl acetate soluble extract of A. macrocephala Koidz. possessed antitumorous and antimicrobial agents

• Korean Journal of Plant Resources
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• v.13 no.2
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• pp.118-123
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• 2000

This study was conducted to determine the appropriate fertilization level for growing one year old rhizome of Atractylodes macrocephala KOIDZ. According to N and K amounts were increased, the growth of above-ground parts was decreased but P was increased at this time. Based on N-P-K levels, application of 8-9-3 kg/10a was the most effectiveness for the growth of above-ground part, and 8-3-3 kg/10a for that of underground part. It can be estimated that 6-4-6 kg/10a treatment based on N-P-K may be the optimum fertilization level to obtain the highest yield of Atractylodes macrocephala KOIDZ using one year old rhizome.

• The Korean Journal of Food And Nutrition
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• v.14 no.1
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• pp.15-19
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• 2001

An attempt was made in this study to analyze volatile flavor components of A. macrocephala Koidz. and A. lanacea DC. (Atractylodes japonica Koidzumi). Essential oils in A. macrocephala Koidz. and. A. lanacea DC. were isolated by a simultaneous steam distillation and extraction(SDE) method using n-pentane/diethy ether as solvent. A total of 30 and 28 components were identified by GC/MS from the essential oils of A. macrocephala Koidz. (18 hydrocarbons. 2 carbonyls, 5 alcohols, 5 esters) and A. lanacea DC.(14 hydrocarbons, 6 carbonyls, 4 alcohols, 3 esters, 1 acids), respectively. The major volatile flavor components in A. macrocephala Koidz. and A. lanacea DC. were furanodiene(27.9%, 15.7%), $\alpha$-cyperone(8.1%. 22.5% ), alloaromadendrene(2.9%, 4.7% ), (1,1-biphenyl)-4-carbon aldehyde 0%, 8.7% ) were found, respectively. Ten components including limonene, p-cymene, p-hymen-8-ol, (1,1-biphenyl)-4-carbox aldehyde were identified in A. lanacea DC, but not in A. macrocephala Koidz. and eight components including $\alpha$-copanene, isocaryophyllene, $\beta$-himahalene. germacrene B were and identified in A. macrocephala Koidz. but not in A. lanacea DC.

• Korean Journal of Plant Resources
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• v.14 no.3
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• pp.188-195
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• 2001

The anatomical characteristics of rhizome, lateral root, stem, leaf, petiole, ovary and leaf epidermis about Atractylodes japonica Koidz. ex Kitam. and A. macrocephala Koidz. were investigated to estimate these taxonomic values. The cross sections of stem and ovary were very similar to two species, therefore these characters were not useful for delimitation of two species, but the size and distribution numbers per 100${\mu}{\textrm}{m}$$^2$of oil cavites in rhizome, the shape of leaf and petiole in cross section, the size of leaf epidermal cell and distribution numbers per 100 ${\mu}{\textrm}{m}$$^2$of stomata were differ from two species, therefore these were thought to be useful characters for delimitation of two species.

• Korean Journal of Medicinal Crop Science
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• v.27 no.6
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• pp.404-411
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• 2019

Background: In September 2017, wilting and rhizome rot symptoms were observed on Atractylodes macrocephala Koidz. in Jecheon-si and Eumseong-gun. This study was carried out to isolate hitherto unidentified pathogenic fungi from A. macrocephala and to test the pathogenicity of isolated fungi against Atractylodes spp. genus such as A. macrocephala, A. japonica, and their interspecific hybrids. Methods and Results: The diseased plants were washed with running tap water, and the boundary between the healthy area and the diseased area was cut while the pathogens were isolated by growing cultures from the diseased areas on Phytophthora semi-selective medium. The internal transcribed spacer (ITS) region of the isolates was used in this study for identification. Test plants were cultivated in the glasshouse at 20℃ - 30℃ for 4 months and then used for pathogenicity test. The pots with plants inoculated with mycelial plugs and zoospores were placed at 25℃ for 48 h in a dew chamber where relative humidity was above 95%, and then moved into the glasshouse at 20℃ - 30℃. The presence or absence of pathogenicity of the strains was determined by evaluating the symptom of plant wilting. The inoculation test was performed in three replicates with a non-treated control. Conclusions: On the basis of results of ITS sequence analysis, the strains isolated from the diseased plants was identified as Phytophthora sansomeana. Biological assay using test plants confirmed the pathogenicity of P. sansomeana against Atractylodes macrocephala. This is the first report of rhizome rot in A. macrocephala caused by P. sansomeana.

• 한국약용작물학회:학술대회논문집
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• 2011.09a
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• pp.374-375
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• 2011

• Korean Journal of Plant Resources
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• v.14 no.2
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• pp.152-156
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• 2001

This study was conducted to investigate the flower organ removal effect on Atractylodes macrocephala Koidz which was introduced from China. The results were summarized as follows; The plant height of Flower Organ Cutting(F.O.N.C.) treatment short by 1.7∼2.5 cm compared to Flower Organ Non-Cutting(F.O.N.C.) treatment. But number of stem and stem diameter of F.0.C treatment were similar to that of F.O.N.C. treatment. The fresh weight of above-ground part of F.O.N.C. treatment was decreased 48∼60% compare to F.O.C. treatments. The later was period of F.O.C., the higher was fresh weight of above-ground part. The growth of underground part was more F.O.C. at July 15 and Aug. 20 treatments than F.O.N.D treatment. The dry rhizome yield of F.O.C. at July 15 treatment was increased 40% compare to F.O.N.D treatment. Essential oil content of F.O.C. at July 15 treatment was increased 12% compare to F.O.N.D treatment.

• Korean Journal of Medicinal Crop Science
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• v.11 no.4
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• pp.311-315
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• 2003

This study was carried out to compare chromosomal characteristics between Atractylodes japonica and A macrocephala. Cytogenetic analysis was conducted based on karyotype analysis and physical mapping using fluorescence in situ hybridization. As a result of karyotype analysis by feulgen staining, somatic chromosome numbers of A. japonica and A. macrocephala were 2n=24. The length. of the mitotic metaphase chromosomes of A. japonica ranged from $0.70\;to\;1.60{\mu}m$ with a total length. of $12.11{\mu}m$ and the homologous chromosome complement comprised six metacentrics, five submetacentrics and one subtelocentrics. On the other hand, the length of the mitotic metaphase chromosomes of A. macrocephala ranged from $0.90\;to\;2.35{\mu}m$ with a total length of $16.58{\mu}m$ and the homologous chromosome complement comprised seven metacentrics and five submetacentrics. The total length of A. japonica chromosomes was shorter than that of A. macrocephala, but A. japonica had one subtelocentrics (chromosomes 4) different from A. macrocepha1a. chromosomes. The F1SH technique using 17S and 5S rDNA was applied to metaphase chromosomes. The signals for 17S rDNA were detected on the telomeric regions of chromosomes 4 and 5 in both A japonica and A. macrocephala. The 5S rDNA signal was found in the short arm of chromosome 1.

• Korean Journal of Medicinal Crop Science
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• v.14 no.2
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• pp.107-112
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• 2006

Two Atractylodes species, A. japonica Koidz. ex Kitam. (AJ) and A. macrocephala Koidz (AM) were used in this study. AJ population had higher amounts of Sesquiterpenoids and stronger tolerance to root rot but less vigor of root growth than AM population. Two populations (AJ and AM) were crossed to make interspecific hybrid population. A total of 98 lines propagated clonally were selected from a cross of AJ and AM, and evaluated for contents of sesquiterpenoids, atractylon (ATLN) and atractylenolide III (AT3) using high performance liquid chromatography (HPLC), and growth characters such as plant height, stem number and root weight. HPLC profiles of the hybrids were compared with those of parent plants, and it demonstrated the production of introgression hybrid by crossing between AJ and AM. Of 98 clonal lines,10 lines were selected by 10% level based on the growth vigor and tolerance to root rot, and AJM2102-51 line showed the heaviest root weight (117.1 g/plant) among them. A total of 98 hybrid lines contained on average $0.16\;{\pm}\;0.10\;mg/g$ of $AT3,\;2.00\;{\pm}\;1.37\;mg/g$ of ATLN, and $2.16\;{\pm}\;1.40\;mg/g$ of total sesquiterpenoids, showing high coefficients of variation (above 65%). Ten lines having high contents of sesquiterpenoids were selected, and AJM2101-15 had the highest amount (9.83 mg/g) of ATLN, and showed 40.8 g/plant of root weight similar to mean value (39.9 g/plant) of hybrid lines. The result showed that the introgression of both characters of vigorous growth from AM and high sesquiterpenoids content from AJ could be possible to make new hybrid lines by crossing between AJ and AM.