Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Occurrence of the Phytophthora Blight Caused by Phytophthora sansomeana in Atractylodes macrocephala Koidz.

Phytophthora sansomeana에 의한 큰꽃삽주 역병 발생 보고

  • An, Tae Jin (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Park, Myung Soo (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Jeong, Jin Tae (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Young Guk (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Yong Il (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Eun Song (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Chang, Jae Ki (Department of Herbal Crop Research, NIHHS, RDA)
  • 안태진 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 박명수 (서울대학교 생명과학부) ;
  • 정진태 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 김영국 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 김용일 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 이은송 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 장재기 (국립원예특작과학원 인삼특작부 약용작물과)
  • Received : 2019.10.17
  • Accepted : 2019.11.26
  • Published : 2019.12.31
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Abstract

Background: In September 2017, wilting and rhizome rot symptoms were observed on Atractylodes macrocephala Koidz. in Jecheon-si and Eumseong-gun. This study was carried out to isolate hitherto unidentified pathogenic fungi from A. macrocephala and to test the pathogenicity of isolated fungi against Atractylodes spp. genus such as A. macrocephala, A. japonica, and their interspecific hybrids. Methods and Results: The diseased plants were washed with running tap water, and the boundary between the healthy area and the diseased area was cut while the pathogens were isolated by growing cultures from the diseased areas on Phytophthora semi-selective medium. The internal transcribed spacer (ITS) region of the isolates was used in this study for identification. Test plants were cultivated in the glasshouse at 20℃ - 30℃ for 4 months and then used for pathogenicity test. The pots with plants inoculated with mycelial plugs and zoospores were placed at 25℃ for 48 h in a dew chamber where relative humidity was above 95%, and then moved into the glasshouse at 20℃ - 30℃. The presence or absence of pathogenicity of the strains was determined by evaluating the symptom of plant wilting. The inoculation test was performed in three replicates with a non-treated control. Conclusions: On the basis of results of ITS sequence analysis, the strains isolated from the diseased plants was identified as Phytophthora sansomeana. Biological assay using test plants confirmed the pathogenicity of P. sansomeana against Atractylodes macrocephala. This is the first report of rhizome rot in A. macrocephala caused by P. sansomeana.

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References

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