• Journal of Ecology and Environment
• /
• 제48권2호
• /
• pp.128-136
• /
• 2024

Background: Prasiola japonica is a freshwater green algae species that can only be seen in Korea and Japan. The various conditions necessary for its growth and reproduction have not been fully elucidated. Therefore, in this study, we aimed to investigate conditions related to the growth and reproduction of P. japonica for the purpose of conserving and producing this species. We first examined differences in growth according to various conditions in different habitats to understand the growth environment of P. japonica. Results: The experimental results revealed that the optimal temperature for growth and reproduction of P. japonica was between 10℃-15℃, and the optimal light intensity was 1,000-1,500 lux. Furthermore, when Provasoli enriched seawater with iodine (PESI) agar was used, the growth of P. japonica was found to be at least 1.5-8 times greater than that of the general Sohan Valley water sample, and it also showed 2-4.5 faster growth rate to reach 30 ㎛. These results emphasize the importance of PESI agar in the culture of P. japonica, and are expected to be helpful in suggesting ways to utilize and conserve P. japonica resources. Conclusions: Through these research findings, we suggest new methods for conserving and producing P. japonica, highlight the importance of preserving the P. japonica ecosystem, and explore ways to utilize P. japonica resources. This research promotes the understanding and protection of P. japonica resources in Korea and beyond, and underscores the need for further research and conservation efforts.

• 한국식품저장유통학회:학술대회논문집
• /
• 한국식품저장유통학회 2003년도 제23차 추계총회 및 국제학술심포지움
• /
• pp.203-204
• /
• 2003

Change in Quality Properties of Doenjang Prepared with the Powder of Paecilomyces japonica and extract of P. japonica by different solvents were investigated during 90 days of fermentation at 20$^{\circ}C$. The moisture content was not significantly different, pH of Doenjang made with P. japonica was lower than control group and decreased continuously according to the fermentation time. Amino nitrogen continuously increased till 60 days and decreased slightly on 90 days. Value of L, a, b in Color decreased with proportion to fermentation period and L, a, b value of Doenjang made with P. japonica was lower and in Particular that of Doenjang made with P. japonica powder was lowest. From the result of sensory evaluation test, the color of control group was similar to “yellow”but that of Doenjang made with powder of P. japonica was close to“dark brown”and that of Doenjang made with extract of P. japonica was darker than that of control group and the preference of dark color was low. Texture was“glossy and smooth”in all and preference was high. In salty taste, Doenjang by P. japonica addition was stronger and Doenjang made with extract was stronger than that made with powder. Doenjang made with powder of P. japonica was weaker than other groups in sweet taste. In flavor and overall Preference, Doenjang made with P. japonica was scored lower slightly in than control group and Doenjang made with powder of P. japonica was the lowest in score.

• 한국식품영양학회지
• /
• 제22권2호
• /
• pp.159-165
• /
• 2009

E. japonica is a well-known medicinal plant in Japan. The leaves of E. japonica were reported to have a hypoglycemic action. However, seeds of E. japonica are discarded and not used. To elucidate for anti-diabetic effects of E. japonica, Type 2 diabetic mice were allocated to control group, E. japonica leaf, and seed extract group. Animals were fed a 2018S Teklad global 18% protein rodent diet. Animals were received daily oral injections of E. japonica leaf or seed extract at a dose of 200 mg/kg body weight for 6 weeks. Body weight, food intake and water intake, and total adipose tissue weight of animals were significantly reduced by feeding of E. japonica leaf extract. All E. japonica extract groups significantly decreased fasting blood glucose, glycosylated hemoglobin levels, size of adipocytes and serum adiponectins. However, they did not have a beneficial effect on the serum triglyceride and cholesterol in the diabetic animals. These results suggest that E. japonica seed and leaf extracts have a antidiabetic effect by controlling of blood glucose and decrease of size of adipocytes in db/db mice and seed extract is more effective in hypoglycemic action than leaf extract.

• 생약학회지
• /
• 제52권3호
• /
• pp.143-148
• /
• 2021

This study was performed to elucidate the inhibitory effects of Alveopora japonica extract on melanin synthesis by measuring the levels of cell viability, mRNA expression, tyrosinase activity, and melanin production in the B16F10 cell line. The effects of A. japonica extract on tyrosinase-related protein 1 (TYRP1), TYRP2, tyrosinase (TYR), and microphthalmia-associated transcription factor (MITF) mRNA expression levels and melanin content were determined. Quantitative real-time RT-PCR show that A. japonica extract decrease the mRNA expression levels of TYRP1, TYRP2, TYR, and MITF in B16F10 cell line, resulting in lower levels of melanin production compared to α-MSH-treated B16F10 cells. Tyrosinase activity assays reveal that A. japonica extract decrease melanin production in B16F10 cells. These results demonstrate the whitening effects of A. japonica extract on B16F10 cells; thus, A. japonica extract is a potent ingredient for skin whitening. Further research is needed on the mechanism of action of A. japonica extract. Such research will benefit not only cosmetics, but also the health food and medical industries.

• 아시안잔디학회지
• /
• 제11권4호
• /
• pp.281-288
• /
• 1997

This study was performed to find out the mineral cycles of sulphur in dynamic grassland ecosystems in a steady state condition at the northwest side on Mt. Kwanak. The experimental results may be summarized on the communities of a Zoysia japonica and a Miscanthus sinensis as follows. As compared with some properties of the surface soils among two semi-natural grasslands, sulphur war greater quantity in a Miscanthus sinensis than in a Zoysia japonica on Mt. Kwanak . For the case of steady production and release, the raion of annual mineral production to the amount accumulated on the top of mineral soil in a steady state provides the estimates of release constant k. The release constants on sulphur of the litter were 0.54 in a Zoysia japonica and 0.36 in a Miscanthus sinensis grassland. The half times of S required for the release or accumulation of the litter on the grassland were 1.28 years in a Zoysia japonica and 1.93 years in a Miscauthus sinensis The amounts of annual cycles for sulphur in the grassland ecosystem under the steady-state conditions were 20.65g /$m^2$ in a Zoysia japonica and 26.28g /$m^2$ in a Miacanthus sinensis grassland. Key words: Zoysia japonica Miscanthus sinensis Mt. Kwanak, Sulphur, Mineral cycles.

• 대한수의학회지
• /
• 제54권1호
• /
• pp.31-38
• /
• 2014

Cordyceps is a fungus used as a traditional medicine in China, Japan, and Korea. Paecilomyces (P.) japonica is a new cordyceps that was recently cultivated on silkworm pupae in Korea. The present study evaluated the toxicological effects of P. japonica in rats. Forty rats were treated with oral doses of P. japonica (0, 20, 100, or 500 mg/kg/day) for 4 weeks. Twenty additional rats were treated with 0 or 500 mg/kg/day of P. japonica for 4 weeks and then maintained for 2 weeks without treatment. Clinical signs, body weight, food and water consumption, and organ weight as well as hematology, serum biochemistry, and histopathology data were examined. Body weight gain of the group treated with 500 mg/kg/day was significantly reduced. Microscopically, karyomegaly, single cell necrosis, and mitosis were observed in the renal tubular epithelium of all treated groups. In conclusion, P. japonica caused a reduction of body weight and renal injury in rats. The no observed adverse effect level (NOAEL) of P. japonica was less than 20 mg/kg/day.

• IMMUNE NETWORK
• /
• 제7권2호
• /
• pp.95-100
• /
• 2007

Background: A red seaweed, Callophyllis japonica has been traditionally eaten in the oriental area. In a recent study, it has been demonstrated that the ethanol extract of C. japonica have antioxidant activity. However, there are few studies about the effects of C. japonica on the function of immune cells. We investigated the immunomodulatory effects of C. japonica on the function of dendritic cells, the potent antigen-presenting cells. Methods: Bone marrow-derived dendritic cells (DCs) were used and the viability was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay and trypan blue exclusion test. Cytokine and nitric oxide (NO) levels were determined by using ELISA and Griess reagent, respectively. The expression levels of DC surface markers were measured by flow cytometric analysis. Results: C. japonica ethanol extract did not significantly affect the DCs viability and the IL-12 production from DCs, irrespective of the presence of lipopolysaccharide (LPS). In addition, it did not significantly change the expression of DC surface markers. However, C. japonica ethanol extract significantly inhibited the LPS-induced NO production and also increased the proliferation of allogeneic lymphocytes activated by DCs. Conclusion: Our data suggests that C. japonica ethanol extract enhances the proliferation of allogeneic lymphocytes activated by DCs which is associated with inhibition of NO production from DCs induced by LPS.

• ALGAE
• /
• 제21권3호
• /
• pp.287-294
• /
• 2006

Neosiphonia japonica is a rhodomelacean red alga that occurs in Korea, Japan, China, far-east Russia, northwest America, and New Zealand. Although it is distinguished by a bush-like habit having four pericental cells with cortication and numerous branches on axes, the taxonomy of N. japonica is still problematic. To investigate the taxonomy and phylogeny of the species, we analyzed rbcL and phycoerythrin (cpeA/B) genes from 19 samples of N. japonica and putative relatives. Phylogenetic trees from both genes show that N. japonica from Korea, Japan, New Zealand, and USA is clearly separated from N. decumbens, N. harlandii, and N. flavimarina from the Pacific Ocean. Instead, N. harveyi from the Atlantic Ocean was more related to N. flavimarina than to N. japonica. This result supports morphological and distributional differences between N. japonica and N. harveyi. However, the close relationship between these species suggests that they might have a recent most common ancestor. This is the second report to use the cpeA/B gene for evaluating species diversity in the Rhodophytes.

• 아시안잔디학회지
• /
• 제9권4호
• /
• pp.265-273
• /
• 1995

This investigation is carried out to clarify the cycles of nitrogen in the grassland ecosystems of Zoysia japonica and a Miscanthus sinensis on Mt. Kwanak. The hasic differential equation for the rate of change of nitrogen storage is illustrated hy huild-up and turnover of organic nitrogen, particularly in the ecosystems. The turnover velocity fractions of nitrogen for the Z. japonica and M .sinensis grasslands were k= 0. 181 and k=0. 166, respectively. The times required to reach 50, 95 and 99 percent of the steady state levels and turnover values of nitrogen on the grassland floors were 3.85, 16.67 and 27.78 years in the Z japonica grassland and 4.08, 17.65 and 29.41 years in the M sinensis grassland. The amount of annual cycles of nitrogen are 560.2 g /$m^2$ in the Z.japonica grassland and 654.1 g /$m^2$ in the M. sinensis grassland. Key words : Zsysia japonica Alisca nthus sinensis, Mt. Kivanak, Nitrogen cycle.

• 아시안잔디학회지
• /
• 제11권4호
• /
• pp.289-294
• /
• 1997

The cycles of heavy metals, mercury, lead and cadmium, were investigated on in dynamic grassland ecosystems at a steady state in Zoysia japonica and Miscanthus sinensis in Mt. Kwanak, Korea. Estimates of decay constants of heavy metals based on experimental and methomatical model, were Hg 0.14, Pb 0.17 and Cd 0.41 of Z. japonica grassland, and Hg 0.33, Pb 0.13 and Cd 0.56 of M sinensis grassland. The durations of reaching half of initial amounts in Z. japonica and M. sinensis grassland, were Hg 4.95, Pb 4.07 and Cd 1.69 years, and Hg 2.10, Pb 5.33 and Cd 1.24 years respectively. Times needed for 99% decomposed were longer in Z. japonica than M. sinensis grassland. Decay velocity of constituents of surface soil layers were more rapidly in M. sinensis than Z. japonica grassland. Key words: Cycles of heavy metals, Mercury, Lead, Cadmium, Zoysia japonica. Miscanthus sineusis, Mt. Kwanak, Decay constants.