• Title/Summary/Keyword: A. fauriei

Search Result 47, Processing Time 0.023 seconds

Production and Isolation of IgY Antibody Raised Against a Lectin Obtained from Maackia fauriei (Maackia fauriei 유래 렉틴에 대한 IgY 항체의 생성 및 분리)

  • Chung Young Yun;Jung Eui Cha;Lee Hyun Jung;Kim HaHyung
    • YAKHAK HOEJI
    • /
    • v.49 no.1
    • /
    • pp.6-10
    • /
    • 2005
  • Immunoglobulin Y (IgY) obtained from chicken as the immunization host brings several advantages to antibody production, such as improved yield, lower cost, longer stability and higher specificity than mammalian immunoglobulin. In the present study, we attempted to produce IgY against a sialic acid-binding lectin, Maackia fauriei agglutinin (MFA), from egg yolk of white Leghorn hens. For the isolation of IgY from egg yolk, we applied a water dilution method. The weekly yield of IgY was determined by enzyme-linked immunosorbent assay, with a final yield of anti-MFA IgY from total IgY of approximately $1\%$. The yielded IgY were used to prepare IgY-affinity column conjugated with CNBr-activated Sepharose 4B, which resulted in the lectin being successfully purified in a single step from Maackia fauriei. This purified lectin exhibited the same hemagglutination activity as lectin purified using conventional purification methods.

Antioxidative Activities and Inhibition Effects on Oxidative DNA Damage of Valeriana fauriei (쥐오줌풀의 항산화 및 산화적 DNA 손상 억제 활성)

  • Park, Jae Ho;Jang, Tae Won;Lee, Seung Hyun
    • Korean Journal of Medicinal Crop Science
    • /
    • v.24 no.6
    • /
    • pp.464-470
    • /
    • 2016
  • Background: Valeriana fauriei (Valerianaceae) has been used to as a traditional medicine to treat a variety of symptoms, including headache, insomnia, hypertension, and menstrual irregularity. However, the present study investigates the species' antioxidant activity and its inhibition of oxidative DNA damage, which have yet to be studied. Methods and Results: The antioxidant activity was assessed using radical scavenging assays with 1,1-diphenyl-2-picryl hydrazyl (DPPH) and, 2, 2'-azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) and a reducing power assay. The total phenol content was also analyzed, and phenolic compounds were detected using HPLC/UV, whereas the inhibitory effect of Valeriana fauriei on oxidative DNA damage was measured using ${\phi}-174$ RF I plasmid DNA cleavage assay. The DPPH and ABTS radical scavenging activity were $75.17{\pm}3.55%$ and $95.83{\pm}0.63%$, repectively, and the reducing power was $93.14{\pm}1.74$ at $200{\mu}g/m{\ell}$. The total phenol content was $10.24{\pm}0.04mg/g$, whereas chlorogenic acid, catechin, caffeic acid and epicatechin were identified using HPLC/UV, and the ${\phi}-174$ RF I plasmid DNA cleavage assay indicated that V. fauriei provided protection against oxidative damage. Conclusions: The results of the present study suggest that V. fauriei has powerful antioxidant activity that can provide protective effects against the oxidative DNA damage caused by free radicals. The species, therefore, provides a valuable resource for the development of natural pharmaceutical to treat aging, cancer, and degenerative diseases.

Identification of Aromatic Components and Physiological Activities of Valeriana fauriei Essential Oil (쥐오줌풀 정유의 향기성분 동정과 생리활성 효과 연구)

  • Ji-Eun Jung;Sook-Heui Jung
    • Journal of the Korean Applied Science and Technology
    • /
    • v.41 no.3
    • /
    • pp.733-744
    • /
    • 2024
  • This study extracted essential oil from the native Korean plant Valeriana fauriei and performed fragrance component analysis, antioxidant (DPPH, ABTS), cell viability (MTS), and anti-inflammatory (Nitric oxide) experiments based on the analysis results. The fragrance component analysis revealed that the major effective component of Valeriana fauriei, bornyl acetate, was present at a high content of 47.88%, compared to other regions. Additionally, patchouli alcohol (18.9%), camphene (11.37%), α-Pinene (5.44%), and D-limonene (1.11%) were identified. The antioxidant activity showed that the DPPH radical scavenging activity was 73.62% at 250 µl/ml, and the ABTS radical scavenging activity was 82.17% at 250 µl/ml. At a concentration of 5 µl/ml, which did not exhibit cytotoxicity, the NO production inhibition rate decreased by 62.02% compared to the control group. Through these findings, the potential for the application of Valeriana fauriei essential oil in functional products has been scientifically validated, contributing to research utilizing Valeriana fauriei essential oil.

Juncus fauriei Lév. & Vaniot (Juncaceae): a new record in Korea (검정납작골풀(골풀과): 한국 미기록 식물)

  • Kim, Sungmin;Cho, Yanghoon;Kim, Jonghwan;Shim, Sangdeug;Kim, Sangtae
    • Korean Journal of Plant Taxonomy
    • /
    • v.42 no.4
    • /
    • pp.330-334
    • /
    • 2012
  • Distribution of Juncus fauriei L$\acute{e}$v. & Vaniot were restricted in Japan and Russia in previous literatures. We found its new distribution in the Korean peninsula: wet meadows of sandy seashore in Ha-Jo-Dae, Kangwon province. The plants have flattened stems (usually coiled) and black-colored margin in tepals. These are key characters to distinguish J. fauriei from J. decipiens, J. setchuensis, J. haenkei, and J. brachyspathus, which are closely related Korean taxa in the section Juncotypus. We provide description, distribution, and detailed photos of J. fauriei, and key for Korean taxa of sect. Juncotypus. The plant is named as "Geom-Jeong-Nap-Jak-Gol-Pul" in Korean.

Two unrecorded fern species from Korean flora: Cyclosorus penangianus (Hook.) Copel. (Thelypteridaceae) and Pteris fauriei Hieron. (Pteridaceae) (한국산 미기록 양치식물 : 큰별고사리 [Cyclosorus penangianus (Hook.) Copel.] (처녀고사리과), 오름깃고사리 [Pteris fauriei Hieron.] (봉의꼬리과))

  • Moon, Myung-Ok;Yun, Na Rae;La, Eun Hwa;Lim, Jina;Kim, Dae-Shin;Kim, Chan-Soo
    • Korean Journal of Plant Taxonomy
    • /
    • v.44 no.4
    • /
    • pp.257-260
    • /
    • 2014
  • While preparing a floristic study of Korean Pteridophytes, we discovered the following two unrecorded species from Jeju Island: Cyclosorus penangianus (Hook.) Copel. and Pteris fauriei Hieron. C. penangianus can be distinguished from other Korean congeners of the genus Cyclosorus by having exindusiate sori, toward with sinus of ultimate segments of veinlets more than 4. P. fauriei can be distinguished from other Korean congeners of the genus Pteris by having ultimate segments with entire margins, veins reaching to leaf margin and membraneous petiolar scales.

Effect of Temperature on the Growth of Korean Valerian (Valeriana fauriei var. dasycarpa HARA) (온도가 한국산 쥐오줌풀의 생육에 미치는 영향)

  • Lee, Jong-Chul
    • Korean Journal of Medicinal Crop Science
    • /
    • v.3 no.2
    • /
    • pp.77-80
    • /
    • 1995
  • This study was carried out to know the effect of air temperature on photosynthesis and growth of Korean Valerian (valeriana fauriei var. dasycarpa HARA). The results are as follows; 1. Photosynthesis of V fauriei had highly significant relation to temperature in a quadratic regression mo­del, from which the optimum temperature for the plant growth were estimated to be $17.7^{\circ}C$. During the day­time in the field condition, the photosynthesis rate was highest at 9 a.m, then reduced to minimum at 2 p.m., and increased again thereafter. 2. Number of stomata was about $25/mm^2$ on the upper side and $85/mm^2$ on the lower side of the leaf, and the size was $21{\sim}30/{\mu}m$ in diameter. 3. A highly significant quadratic regression was noted between temperature and leaf width or root weight of V. fauriei. It was estimated from the regression equation that the optimum temperature for root growth was $20.3^{\circ}C$.

  • PDF

Effects of Light Intensity and Temperature on Growth and Root Yield of Valeriana fauriei var. dasycarpa HARA (쥐오줌풀의 생육 및 뿌리수량에 미치는 광도와 온도의 영향)

  • Lee, Jong-Chul;Cho, Chang-Hwan;Ahn, Tae-Jin;Choi, Young-Hyun
    • Korean Journal of Medicinal Crop Science
    • /
    • v.4 no.1
    • /
    • pp.7-11
    • /
    • 1996
  • This study was conducted to know the effects of light intensity and temperature on growth of V. fauriei plants. Photosynthesis of V. fauriei had highly significant relations to light intensity and temperature in a quadratic regression model, from which the optimum light intensity and temperature for the plant growth were estimated to be 40,000lux and $17.7^{\circ}C.$ Root was produced less by shading at Jinbu where is located in alpine region, but root yield is increased by shading at Umsong where is located in plane region. Roots were produced more in Jinbu than in Umsong. A highly significant quadratic regression was noted between temperature and leaf width or root weight of V. fauriei. It was estimated from the regression equation that the optimum temperature for root growth was $20.3^{\circ}C.$

  • PDF

Differences of Major Compounds in Valerian fauriei var. dasycarpa HARA and Valerian officinalis L. Grown at Different Places in Korea (광릉쥐오줌풀과 넓은잎쥐오줌풀의 산지별 유효성분의 변화)

  • Cho, Chang-Hwan;Choi, Young-Hyun;Kim, Kun-Soo;Ahn, Tae-Jin;Han, Ok-Kyu
    • Korean Journal of Medicinal Crop Science
    • /
    • v.4 no.2
    • /
    • pp.109-113
    • /
    • 1996
  • This study was conducted to know the effect of cultivation area with different sea levels on essential oil content and its composition and content of major active compounds in Valerian fauriei var. dasycarpa HARA and Valerian officinalis L. The total contents of essential oil were not different according to the locality in both valerian, however, its compositions were varied. The major components in V. fauriei were detected bornyl acetate and sesquiterpene alcohol in three cultivation areas. The major compounds were borneol, borney acetate, ${\beta}\;-sesquiohellandrene$ in V. officinalis other. Among the valepotrate components known as major pharaceutically active components in Europe and Nepal valerian, valtrate was detected in a small amount in V. fauriei, but its quantity was not changed according to the cultivation area.

  • PDF

Difference of Essential Oil in Korean Valerian Root (Valeriana fauriei var. dasycarpa Hara) Grown at Various Locality in Korea (광릉쥐오줌풀의 산지별 정유성분 비교)

  • Lee, Jong-Chul;Kim, Young-Hoi
    • Korean Journal of Pharmacognosy
    • /
    • v.26 no.2
    • /
    • pp.175-178
    • /
    • 1995
  • The study was conducted to investigate the effect of cultivation area with different sea levels on essential oil in the root of Valeriana fauriei var. dasycarpa Hara (Valerianaceae). The total content of the essential oil was not different in various cultivation area, however, its composition varied. The major components were bornyl acetate and sesquiterpene alcohol in three cultivation areas. Among the valepotrate components known as major active components in Europe and Nepal valerian, valtrate was detected in a small amount, but the quantity was not changed.

  • PDF