The objectives or this study were to improve duck meat qualify during storage period in onion-red ducks which contained antioxidants. Experimental animals (1 day old, Cherry Valley $F_{1}$, 360 chicks) were randomly assigned in eight treatment group. Treatments were control, 3%, 6% of chopped onion-fed, 5%, 10% of onion extract fed, 6% of onion meal, 3%, 6% of fermented onion-fed. pH was significantly high (P<0.05) in treatment group after 3 day storage in pectoralis muscle. Meat color was low in r* and a* value. TBA value was not different in day 1, was significantly low (P<0.05) 6% chopped and 6% fermented group after day 3 storage. Also, TBA value was significantly high (P<0.05) in control group and was significantly low in 3% chopped, 5%, 10% extract after day 6 storage. VBN value was lower in all treatment groups except 5% ferment group than control group. Meat order was high score in 3% and 6% chopped group and duck specific smell was reduced in all treatment groups. Meat appearance was higher score in treatment groups than in control group during 9 day storage. In result, onion feeding was reduce duck specific smell and improve meat quality during long period storage in ducks.
Four experiments were conducted to investigate the association of yolk cholesterol level with egg trails, serum cholesterol level, body weight (BW) and breed differences and other variations. Athens Canadian Randombred (ACRB), a commercial layer breed (CL) and two commercial broiler breeds (BR1 and BR2) were used for this studies. Egg weight of CL was heavier by 8.7g per egg than that of ACRB, but the yolk percentage and yolk cholesterol were lower by 7.8% per egg and 5.5mg per g yolk than those of ACRB, respectively. Yolk cholesterol content had no significant relation with the average egg weight or yolk weight in ACRB, but was significantly related with yolk fat level. However, yolk cholesterol level in CL showed inverse relationship with other egg traits compared to that of ACRB. In the CL female group, the association of yolk cholesterol level with BW was significant(P$\leq$0.05) at 25 weeks of age, but was not significant different at 26 wks of age. The relationship of yolk cholesterol with serum was not significantly different at 25 weeks of age, whereas it showed significant negative(-) correlation coefficient(P$\leq$0.01) at 26 wks of age. The association of yolk cholesterol level with egg age in CL population was not high comared to that of other treatments. The serum cholesterol content of CL was not significantly different with BR1 and BR2 populations at 25 weeks of age, but was significantly(P$\leq$0.05) higher than that of BR1 or BR2 at 26 weeks old.
The purpose of this study was to assess the genetic variation and establish the relationship amongst breeds and strains using 7 chicken specific microsatellite markers. A total of 317 DNA samples from four Korean native chicken (KNC) strains (KR: Korean Native Red chicken strain, KY: Korean Native Yellow chicken strain, KL: Korean Native Black chicken strain, KO: Ogol chicken strain) and three introduced endemic chicken breeds (LE: Leghorn chicken breed, RI: Rhode Island Red chicken breed, CO: Cornish chicken breed). The size of microsatellite markers was decided using GeneMapper Software (v.4.0) after being analyzed using an ABI 3130 Genetic Analyzer. Frequencies of microsatellites markers were used to estimate heterozygosities and genetic distances. The lowest distance (0.074) was observed between the KY and KL breeds and the highest distance (0.779) between the KL and LE breeds. The KNC strains (KR, KY, KL) have comparatively near genetic distance each other. On the other side, each individual was not ramified to different groups and were spread evenly in phylogenetic dendrogram about all the KNC of each strain populations. But the endemic breed populations (LE, RI, CO) were ramified to different groups. The microsatellite polymorphism data were shown to be useful for assessing the genetic relationship between Korean native strains and other foreign breeds.
The objective of the present study was to compare of the effects of four molting methods on performance of laying hens. In total, 120 White Leghorn layers (62 weeks of age) were used. The four molting methods consisted of 10 days of feeding and the first 3 days of water withdrawal (FW1, 2) followed by ad libitum access to a layer diet 1 and 2 from day 11; 28 days of restricted feeding (NFW1); 28 days without withdrawing their feed (NFW2). Egg production of postmolt decreased until $3{\sim}4$ days at FW1 and FW2 treatments and until $16{\sim}18$ days at NFW1 and NFW2. 50% recovery of egg production were 7, 6, 7 and 5 week at FW1, FW2, NFW1, and NFW2 treatments, respectively. At day 14 of postmolt, the weight of livers, hearts, and oviducts decreased at molting treatments (P<0.05). Egg quality was improved on egg weight, eggshell thickness, eggshell break strength, and Haugh unit except egg yolk color (P<0.05).
To predict the influence of volcano eruption on agriculture in South Korea we evaluated the dispersal ranges of the volcanic ashes toward the South Korea based on the possibilities of volcano eruption in Mt. Baekdu. The possibilities of volcano eruption in Mt. Baekdu have been still being intensified by the signals including magmatic unrest of the volcano and the frequency of volcanic earthquakes swarm, the horizontal displacement and vertical uplift around the Mt. Baekdu, the temperature rises of hot springs, high ratios of $N_2/O_2$ and $_3He/_4He$ in volcanic gases. The dispersal direction and ranges and the predicted amount of volcanic ash can be significantly influenced by Volcanic Explosivity Index (VEI) and the trend of seasonal wind. The prediction of volcanic ash dispersion by the model showed that the ash cloud extended to Ulleung Island and Japan within 9 hours and 24 hours by the northwestern monsoon wind in winter while the ash cloud extended to northern side by the south-east monsoon wind during June and September. However, the ash cloud may extent to Seoul and southwest coast within 9 hours and 15 hours by northern wind in winter, leading to severe ash deposits over the whole area of South Korea, although the thickness of the ash deposits generally decreases exponentially with increasing distance from a volcano. In case of VEI 7, the ash deposits of Daejeon and Gangneung are $1.31{\times}10^4g\;m^{-2}$ and $1.80{\times}10^5g\;m^{-2}$, respectively. In addition, ash particles may compact close together after they fall to the ground, resulting in increase of the bulk density that can alter the soil physical and chemical properties detrimental to agricultural practices and crop growth.
This study was performed to validate the procedure of transarterial embolization of the renal artery (TAE-RA) using iohexol-ethanol solution in dogs with unilateral experimental hydronephrosis and to evaluate the embolized kidney using B-mode ultrasonography and selective angiography. Experimental hydronephrosis was induced by ligation of unilateral ureter in 12 dogs. Ultrasonographic findings revealed that size of the kidney was significantly increased at 9 days and 17 days and the length of renal cortex was significantly decreased at 17 days after ligation of the unilateral ureter and it was in accordance with dilation of ipsilateral renal pelvis. No significant change of BUN, creatinine, ALT, calcium, and phosphorus was found immediately after unilateral experimental hydronephrosis. Therefore, it was concluded that unilateral hydronephrosis was established in 12 dogs at 17 days after ligation of ureter. Renal artery embolization was performed using selective catheterization in the hydronephrotic kidney of seven dogs and EKG, $SpO_2$, body temperature, pulse, and respiratory rate were within normal ranges during procedures. Iohexol-ethanol solution was used as embolic material. Average ethanol dose for renal artery embolization was $1.94{\pm}1.24ml/kg$. There were no dogs expired after TAE-RA and no side effects associated with regurgitation of iohexol-ethanol solution. Revascularization of renal artery was not found in angiography in dogs treated by TAE-RA at immediately after TAE-RA and 14 days after TAE-RA. Ultrasonographically, the mean longitudinal length of the embolized kidney decreased significantly at 2 and 3 months after TAE-RA compared to that of contralateral normal kidney. In summary, marked shrinkage of the embolized kidney was observed in dogs with unilateral experimental hydronephrosis treated by TAE-RA with iohexol-ethanol and no adverse effects were observed throughout the observation period. It is concluded that TAE-RA with iohexol-ethanol solution is a viable alternative to nephrectomy in dogs with unilateral hydronephrosis.
To estimate the possible amount of weed-crop competitions under the various cropping patterns of two rice cultivars of different type, comparisons between weedy check and weed-free plots in plant height, tillering number, and plant weight were investigated. Fluctuations in Importance Value, competition value, and net productivities were used to discuss the crop-weed competition, respectively. As a result, differences in dominant weed species, competitive crop variety, most effective cropping pattern, and crop yielding characteristics under the different weed competitions were investigated, respectively.
Background: Patients with cardiac diseases who have structural defects in their heart bring about metabolic insult such as preoperative acid-base imbalance. Cardiac operation requires many nonphysiologic procedures such as extracorporeal circulation, hypothermia, and hemodilution. We studied the acid-base status of surgical heart diseases pre-operatively, during extracorporeal circulation, and post-operatively and researched the treatment indications of acid-base disturbances. Material and Method: From January 1997 to May 1999, fifty two cases of open heart surgery were carried out under extracorporeal circulation, which divided into a set of pediatric and adult groups, congenital and acquired groups, non-cyanotic and cyanotic groups, The $\alpha$ -stat arterial blood gas analysis was done in each group during the preoperative period, during the operation with extracorporeal circulation, and during the postoperative period. Result: Before surgery, all patients present metabolic acidosis, PaO2 was low in adult group and acquired group and compensatory respiratory alkalosis was noted in cyanotic group. During extracorporeal circulation, adult group revealed alkalosis and normal in acquired group. Pediatric group presents low Pa$CO_2$, metabolic acidosis and respiratory alkalosis. Congenital group and non-cyanotic group showed non-compensatory alkalosis trend and non-compensatory respiratory acidosis were observed in cyanotic group during extracorporeal circulation. Postoperative acid-base status of adult group was recovered to normal and the standard bicarbonate was increased in the acquired group. All of the pediatric, congenital non-cyanotic, and cyanotic groups revealed the lack of buffer base.
These studies were carried out to improve the reproductive efficiency through embryos transfer of Hanwoo IVM/IVF embryos. Following routine IVM/IVF procedure, oocytes and zygotes were cultured far 40 to 44 h in CRlaa medium with BSA. Then 2 to 8-cell embryos were removed the cumulus cell and were cultured in CRlaa medium containing 10% fatal bovine serum and 2.5 mM taurine in 5% $O_2$ and 5% $CO_2$ at 38.5$^{\circ}C$. The fresh embryos of the morulae and blastocysts cultured for 6 to 9 days in vitro or the frozen-thawed embryos were transferred into recipients. The pregnancy rates of the blastocyst produced for 6, 7, 8, and 9 days in vitro culture were 59.4, 68.2, 66.0 and 100%, respectively. In the developmental stage, pregnacy rates of early blastocysts (61.1%), blastocysts(64.7%) and expanded blastocysts(69.5%) were higher than that of morulae stage(20.0%). The pregnancy rates according to the corpus luteum grades of A, B and C in recipients were 73.6, 62.9 and 50.0%, respectively. Effects of donor-recipients synchrony of after day 2, 1 and 0, before day 1 and 2 on the pregnancy rates were 35.7, 65.5, 72.6, 67.9 and 60.0%, respectively. Pregnancy rates of the body condition score of recipients $\leq$2(71.3%) were higher than those of $\geq$3.0 score(40.0%). The pregnancy rates according to the parity of recipients when embryo was transferred to cow(70.6%) was higher than in heifer(59.1%). The pregnancy rates according to hormone treatment before embryo transfer were 69.9% in hCG + GnRH administration group and 63.0% in control group. Fresh and frozen-thawed embryos on the pregnancy rates were 70.6 and 36.4%, respectively. Pregnancy rates in single and AI+single was 90.0% and 64.8%. Pregnancy rates in twin induction was better than in single. These results indicate that pregnancy rates after transfer were affected on the embryo ages, donor-recipient synchrony, body condition score of recipients, corpus luteum status, parity and hormone treatment to recipients.
A total of 1,200 eggs obtained from 312-day-old Hy-line Brown laying breeder hens and 319-day-old Hy-line Brown commercial laying hens (600 eggs obtained from each one) at same day were used to investigate the effects of egg storage, storage temperature, and insemination of hens on the change of albumen height, Haugh unit(HU), albumen pH, shell strength, and yolk color. Eggs were stored up to 14 days after lay at $3^{\circ}C$ or $10^{\circ}C$ and sampled one day after stored and then 24 hours interval. Longer periods of storage resulted in lower albumen height and HU at both storage temperatures, but in higher albumen pH. The eggs stored at $3^{\circ}C$ were significantly (P<0.05) higher in HU and lower in albumen pH than the ones stored at $10^{\circ}C$. The eggs obtained from the non-inseminated-hens were significantly (P<0.05) higher in albumen height, HU, and albumen pH than the eggs obtained from the inseminated-hens. Whereas, the eggs obtained from the non-inseminated-hens in the mean albumen pH of eggs stored at $3^{\circ}C$ were significantly (P<0.05) higher than the ones obtained from the inseminated-hens, but the mean albumen pH of eggs stored at 10^{\circ}C did not differ each other. The mean shell strength of the eggs obtained from the inseminated-hens was significantly (P<0.05) stronger than that of the eggs obtained from the non-inseminated-hens at both storage temperatures. Albumen height and albumen pH were negatively correlated $(P<0.01\~0.001)$ in both inseminated and non-inseminated-hen's egg groups. The degree of yolk colors was not significantly changed overall of the experimental periods in both storage temperatures. The study suggests that the change of egg freshness such as albumen height and HU is relatively more associated with storage period and storage temperature than insemination or non-insemination of hens.
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