• BMB Reports
• /
• v.54 no.2
• /
• pp.118-123
• /
• 2021

The bacterial effector protein RavZ from a pathogen can impair autophagy in the host by delipidating the mammalian autophagy-related gene 8 (mATG8)-phosphatidylethanolamine (PE) on autophagic membranes. In RavZ, the membrane-targeting (MT) domain is an essential function. However, the molecular mechanism of this domain in regulating the intracellular localization of RavZ in cells is unclear. In this study, we found that the fusion of the green fluorescent protein (GFP) to the MT domain of RavZ (GFP-MT) resulted in localization primarily to the cytosol and nucleus, whereas the GFP-fused duplicated-MT domain (GFP-2xMT) localized to Rab5- or Rab7-positive endosomes. Similarly, GFP fusion to the catalytic domain (CA) of RavZ (GFP-CA) resulted in localization primarily to the cytosol and nucleus, even in autophagy-induced cells. However, by adding the MT domain to GFP-CA (GFP-CA-MT), the cooperation of MT and CA led to localization on the Rab5-positive endosomal membranes in a wortmannin-sensitive manner under nutrient-rich conditions, and to autophagic membranes in autophagy-induced cells. In autophagic membranes, GFP-CA-MT delipidated overexpressed or endogenous mATG8-PE. Furthermore, GFP-CA△α3-MT, an α3 helix deletion within the CA domain, failed to localize to the endosomal or autophagic membranes and could not delipidate overexpressed mATG8-PE. Thus, the CA or MT domain alone is insufficient for stable membrane localization in cells, but the cooperation of MT and CA leads to localization to the endosomal and autophagic membranes. In autophagic membranes, the CA domain can delipidate mATG8-PE without requiring substrate recognition mediated by LC3-interacting region (LIR) motifs.

• Earthquakes and Structures
• /
• v.5 no.2
• /
• pp.129-142
• /
• 2013

Linear and nonlinear time history analyses have been becoming more common in seismic analysis and design of structures with advances in computer technology and earthquake engineering. One of the most important issues for such analyses is the selection of appropriate acceleration time histories and matching these histories to a code design acceleration spectrum. In literature, there are three sources of acceleration time histories: artificial records, synthetic records obtained from seismological models and accelerograms recorded in real earthquakes. Because of the increase of the number of strong ground motion database, using and scaling real earthquake records for seismic analysis has been becoming one of the most popular research issues in earthquake engineering. In general, two methods are used for scaling actual earthquake records: scaling in time domain and frequency domain. The objective of this study is twofold: the first is to discuss and summarize basic methodologies and criteria for selecting and scaling ground motion time histories. The second is to analyze scaling results of time domain method according to ASCE 7-05 and Eurocode 8 (1998-1:2004) criteria. Differences between time domain method and frequency domain method are mentioned briefly. The time domain scaling procedure is utilized to scale the available real records obtained from near fault motions and far fault motions to match the proposed elastic design acceleration spectrum given in the Eurocode 8. Why the time domain method is preferred in this study is stated. The best fitted ground motion time histories are selected and these histories are analyzed according to Eurocode 8 (1998-1:2004) and ASCE 7-05 criteria. Also, characteristics of both near fault ground motions and far fault ground motions are presented by the help of figures. Hence, we can compare the effects of near fault ground motions on structures with far fault ground motions' effects.

• Current Optics and Photonics
• /
• v.1 no.2
• /
• pp.157-162
• /
• 2017

In large liquid crystal displays, the image quality in an oblique viewing direction is a crucial issue. From this perspective, 8-domain polymer-stabilized vertical alignment (PS-VA) mode has been developed to suppress the color shift in oblique viewing directions, compared to that in 4-domain PS-VA mode. To realize the 8-domain PS-VA, the four domains in a pixel are each divided into two regions, such that applying different electric potentials result in different tilt angles in these two regions, while keeping four azimuthal directions in each domain. However, applying different voltages in a pixel causes drawbacks, such as requiring additional processes to construct a capacitor and a transistor, which will further reduce the aperture ratio. Here we propose a different approach to form the 8-domain, by controlling surface polar anchoring energy and the width of patterned electrodes in two regions of a pixel. As a result, the gamma-distortion index (GDI), measured at an azimuthal angle of $0^{\circ}$, is reduced by about 23% and 8%, compared to that of a conventional 4-domain at polar angles of $30^{\circ}$ and $60^{\circ}$ respectively.

• Molecules and Cells
• /
• v.43 no.7
• /
• pp.662-670
• /
• 2020

We have investigated the involvement of the pre-mRNA processing factor 4B (PRP4) kinase domain in mediating drug resistance. HCT116 cells were treated with curcumin, and apoptosis was assessed based on flow cytometry and the generation of reactive oxygen species (ROS). Cells were then transfected with PRP4 or pre-mRNA-processing-splicing factor 8 (PRP8), and drug resistance was analyzed both in vitro and in vivo. Furthermore, we deleted the kinase domain in PRP4 using Gateway™ technology. Curcumin induced cell death through the production of ROS and decreased the activation of survival signals, but PRP4 overexpression reversed the curcumin-induced oxidative stress and apoptosis. PRP8 failed to reverse the curcumin-induced apoptosis in the HCT116 colon cancer cell line. In xenograft mouse model experiments, curcumin effectively reduced tumour size whereas PRP4 conferred resistance to curcumin, which was evident from increasing tumour size, while PRP8 failed to regulate the curcumin action. PRP4 overexpression altered the morphology, rearranged the actin cytoskeleton, triggered epithelial-mesenchymal transition (EMT), and decreased the invasiveness of HCT116 cells. The loss of E-cadherin, a hallmark of EMT, was observed in HCT116 cells overexpressing PRP4. Moreover, we observed that the EMT-inducing potential of PRP4 was aborted after the deletion of its kinase domain. Collectively, our investigations suggest that the PRP4 kinase domain is responsible for promoting drug resistance to curcumin by inducing EMT. Further evaluation of PRP4-induced inhibition of cell death and PRP4 kinase domain interactions with various other proteins might lead to the development of novel approaches for overcoming drug resistance in patients with colon cancer.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.44 no.3 s.357
• /
• pp.20-25
• /
• 2007

The periodic domain-inversion in the selective areas of $Ti:LiNbO_3$ Mach-Zender waveguides was performed and band-pass modulators and single sideband (SSB) modulators were fabricated by using domain-reversal. The domain wall velocity was precisely controlled by real-time analysis of a poling-induced response current under an applied voltage. The domain wall velocity was significantly affected by the crystal orientation of the domain wall propagation which influenced the final domain geometry. In a certain case, the decomposition of $LiNbO_3$ crystal was observed, for example, under the condition of too fast domain wall propagation. The fabricated band-pass modulator with a periodic domain-inversion structure showed the maximum modulation efficiency at 30.3 GHz with 5.1 GHz 3dB-bandwidth, and SSB modulator was measured to show 33 dB USB suppression over LSB at 5.8 GHz RF.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.12 no.8
• /
• pp.3384-3390
• /
• 2011

This paper describes a domain decomposition method of parallel finite element analysis for elasto-plastic structural problems. As a parallel numeral algorithm for the finite element analysis, the authors have utilized the domain decomposition method combined with an iterative solver such as the conjugate gradient method. Here the domain decomposition method algorithm was applied directly to elasto-plastic problem. The present system was successfully applied to three-dimensional elasto-plastic structural problems.

• Journal of the Korean Crystal Growth and Crystal Technology
• /
• v.12 no.1
• /
• pp.50-55
• /
• 2002

$Nd_2O_3$0.2~0.5 wt.% doped $LiNbO_3$single crystals were grown by the Czochralski method. The ZnO doping by 2~8 mole% can improve the resistance of optical damage. In this study, Nd : LiNbO$_3$ single crystals with the periodical domain structure were obtained by CZ method.

• Medical Lasers
• /
• v.8 no.1
• /
• pp.32-34
• /
• 2019

Postinflammatory hyperpigmentation (PIH) is a reactive hypermelanosis of the skin that occurs as a result of variable inflammatory processes, such as trauma, and many inflammatory conditions. Although a range of modalities of managing PIH have been reported, the treatment of PIH is challenging. In this report, a patient with PIH was treated using picosecond-domain Nd:YAG lasers. After three sessions of 1,064-nm picosecond-domain Nd:YAG laser treatment, the patient showed almost complete improvement with no remarkable side effects or recurrence over the duration of six months. Overall, 1,064-nm picosecond-domain Nd:YAG laser can be used effectively and safely for treating pigmented lesions in the dermis, particularly PIH, in Asian patients.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2015.10a
• /
• pp.681-684
• /
• 2015

Magnetization is very important in the ferro-magnetic physics and provides useful informations in the application field of magnetic devices. Generally, the only first acquired domain pattern is not helpful to recognize domain pattern. Many images are needed to visualize domain pattern through image processing. These images were obtained a 8-bit digital camera. The operation was the subtraction of pixel values of multi domain imanges from the images with 255 of pixel value, which was obtained in the saturated state of magnetic materials. The magnetic domain images was visualized gradually with increasing the number of subtracion operation. LABVIEW was used as an image processing tool and the optic microscope with a polarizer was used in this experiment.

• Journal of the Korean Mathematical Society
• /
• v.53 no.5
• /
• pp.1057-1075
• /
• 2016

Let R be a ring in which Nil(R) is a divided prime ideal of R. Then, for a suitable property X of integral domains, we can define a ${\phi}$-X-ring if R/Nil(R) is an X-domain. This device was introduced by Badawi [8] to study rings with zero divisors with a homomorphic image a particular type of domain. We use it to introduce and study a number of concepts such as ${\phi}$-Schreier rings, ${\phi}$-quasi-Schreier rings, ${\phi}$-almost-rings, ${\phi}$-almost-quasi-Schreier rings, ${\phi}$-GCD rings, ${\phi}$-generalized GCD rings and ${\phi}$-almost GCD rings as rings R with Nil(R) a divided prime ideal of R such that R/Nil(R) is a Schreier domain, quasi-Schreier domain, almost domain, almost-quasi-Schreier domain, GCD domain, generalized GCD domain and almost GCD domain, respectively. We study some generalizations of these concepts, in light of generalizations of these concepts in the domain case, as well. Here a domain D is pre-Schreier if for all $x,y,z{\in}D{\backslash}0$, x | yz in D implies that x = rs where r | y and s | z. An integrally closed pre-Schreier domain was initially called a Schreier domain by Cohn in [15] where it was shown that a GCD domain is a Schreier domain.